Cloned (Comment) | Organism |
---|---|
gene algD, overexpression of wild-type and mutant enzymes in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C213A | site-directed mutagenesis, 1.8fold increased Vmax compared to the wild-type enzyme | Pseudomonas aeruginosa |
C268A | site-directed mutagenesis, 250fold reduced Vmax, 5fold increased KM, and reduced sensitivity to penicillic acid inactivation compared to the wild-type enzyme | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
penicillic acid | irreversible inactivation | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
GDP-D-mannose | pH 8.0, 5°C, recombinant wild-type enzyme | Pseudomonas aeruginosa | |
0.023 | - |
GDP-D-mannose | pH 8.0, 5°C, recombinant mutant C213A | Pseudomonas aeruginosa | |
0.045 | - |
GDP-D-mannose | pH 8.0, 5°C, recombinant mutant C268A | Pseudomonas aeruginosa | |
0.19 | - |
NAD+ | pH 8.0, 5°C, recombinant mutant C213A | Pseudomonas aeruginosa | |
0.21 | - |
NAD+ | pH 8.0, 5°C, recombinant wild-type enzyme | Pseudomonas aeruginosa | |
0.51 | - |
NAD+ | pH 8.0, 5°C, recombinant mutant C268A | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-D-mannose + NAD+ + H2O | Pseudomonas aeruginosa | the enzyme catalyzes the first committed step in alginate biosynthesis | GDP-D-mannunorate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene algD | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli by gel filtration of enzyme with tightly bound GDP-mannuronic acid and NAD+, dialysis and ion exchange chromatography | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-D-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-D-mannunorate + NADH | - |
? | |
GDP-D-mannose + NAD+ + H2O | the enzyme catalyzes the first committed step in alginate biosynthesis | Pseudomonas aeruginosa | GDP-D-mannunorate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | peptide mapping of recombinant wild-type and mutant enzymes with MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
GDP-mannose dehydrogenase | - |
Pseudomonas aeruginosa |
GMD | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inactivation kinetics of recombinant wild-type and mutant enzymes with penicillic acid | Pseudomonas aeruginosa |