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Literature summary for 1.1.1.132 extracted from

  • Roychoudhury, S.; Chakrabarty, K.; Ho, Y.K.; Chakrabarty, A.M.
    Characterization of guanosine diphospho-D-mannose dehydrogenase from Pseudomonas aeruginosa. Structural analysis by limited proteolyses (1992), J. Biol. Chem., 267, 990-996.
    View publication on PubMed
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General Stability

General Stability Organism
repeated freezing/thawing leads to decrease of activity Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
GMP
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 algD gene overproduced in strain 8822 using a plasmid vector containing algD under control of the tac promoter
-

Reaction

Reaction Comment Organism Reaction ID
GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH + 2 H+ class of 4-electron transfer dehydrogenases Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 presence of 1 mM GDP-mannose, GDP-glucose, GMP, GDP, GTP or cGMP retards tryptic digest of enzyme and protects subsequent loss of enzyme activity, amino-terminal domain contains substrate and cofactor binding sites, carboxy-terminal domain is essential for catalytic activity Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
-70°C, 1-3 mg/ml purified protein, 50 mM triethanolamine acetate, pH 7.6, 1 mM DTT Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + NAD+ + H2O
-
 Pseudomonas aeruginosa GDP-mannuronate + NADH
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas aeruginosa