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Literature summary for 1.1.1.132 extracted from

  • Roychoudhury, S.; Mmay, T.B.; Gill, J.F.; Singh, S.K.; Feingold, D.S.; Chakrabarty, A.M.
    Purification and characterization of guanosine diphospho-D-mannose dehydrogenase. A key enzyme in the biosynthesis of alginate by Pseudomonas aeruginosa (1989), J. Biol. Chem., 264, 9380-9385.
    View publication on PubMed
Search for more literature for 1.1.1.132

Inhibitors

Inhibitors Comment Organism Structure
ATP 26% inhibition at 1 mM Pseudomonas aeruginosa
D-maltose 17% inhibition at 1 mM Pseudomonas aeruginosa
GDP-D-glucose 25% inhibition at 1 mM Pseudomonas aeruginosa
GMP competitive, 52% inhibition at 1 mM; competitive, most potent Pseudomonas aeruginosa
iodacetamide 40% inactivation within 30 min, complete reactivation by dithiothreitol Pseudomonas aeruginosa
iodoacetamide 40% inactivation after 30 min preincubation Pseudomonas aeruginosa
maltose
-
 Pseudomonas aeruginosa
additional information full reactivation by dithiothreitol (25 mM) after p-hydroxymercuribenzoate or iodoacetamide inactivation Pseudomonas aeruginosa
p-hydroxymercuribenzoate 96% inactivation after 5 min preincubation; 96% inactivation within 5 min, complete reactivation by dithiothreitol Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0149
-
 GDP-D-mannose
-
 Pseudomonas aeruginosa
0.0149
-
 GDP-D-mannose 24°C, pH 8.0 Pseudomonas aeruginosa
0.185
-
 NAD+ cosubstrate GDP-D-mannose Pseudomonas aeruginosa
0.185
-
 NAD+ 24°C, pH 8.0 Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 6 * 48000, SDS-PAGE Pseudomonas aeruginosa
290000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa key enzyme in alginate biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 mucoid strain 8821
-
Pseudomonas aeruginosa
-
 enzyme overproduced using a plasmid vector containing algD under control of the tac promoter
-
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pH/heat treatment acetone precipitation HPLC gel filtration chromatography Pseudomonas aeruginosa

Renatured (Commentary)

Renatured (Comment) Organism
inactivation by iodacetamide or p-hydroxymercuribenzoate is completely reversible Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.068
-
-
 Pseudomonas aeruginosa
1068
-
 24°C, pH 8.0 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-D-mannose + NAD+ + H2O
-
 Pseudomonas aeruginosa GDP-D-mannuronate + NADH
-
 ?
GDP-mannose + NAD+ + H2O
-
 Pseudomonas aeruginosa GDP-mannuronate + NADH
-
 ?
additional information key enzyme in alginate biosynthesis Pseudomonas aeruginosa ?
-
 ?
additional information no substrate: D-mannose, UDP-D-mannose, UDP-D-glucose, TDP-D-glucose, CDP-D-glucose Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 48000, SDS-PAGE Pseudomonas aeruginosa
additional information N-terminal amino acid sequence Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 above, 2-3 min, loss of activity Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0227
-
 GMP 24°C, pH 8.0 Pseudomonas aeruginosa