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Literature summary for 1.1.1.132 extracted from

  • Preiss, J.
    GDP-mannose dehydrogenase from Arthrobacter (1966), Methods Enzymol., 8, 285-287.
No PubMed abstract available
Search for more literature for 1.1.1.132

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 GDP-mannose
-
 Arthrobacter sp.
0.084
-
 deoxy-GDP-D-mannose
-
 Arthrobacter sp.
0.26
-
 NAD+ cosubstrate 0.3 mM GDP-mannose Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 NRRL B1973
-
Arthrobacter sp. NRRL B1973
-
 NRRL B1973
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate fractionation Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.39
-
-
 Arthrobacter sp.

Storage Stability

Storage Stability Organism
-15°C, protein concentration above 1 mg/ml, at least 1 month Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Deoxy-GDP-D-mannose + 2 NAD+ + H2O
-
 Arthrobacter sp. deoxy-GDP-D-mannuronate + 2 NADH + 2 H+
-
 r
Deoxy-GDP-D-mannose + 2 NAD+ + H2O
-
 Arthrobacter sp. NRRL B1973 deoxy-GDP-D-mannuronate + 2 NADH + 2 H+
-
 r
deoxy-GDP-D-mannose + NAD+ + H2O
-
 Arthrobacter sp. deoxy-GDP-D-mannuronate + NADH
-
 ?
deoxy-GDP-D-mannose + NAD+ + H2O
-
 Arthrobacter sp. NRRL B1973 deoxy-GDP-D-mannuronate + NADH
-
 ?
GDP-mannose + NAD+ + H2O
-
 Arthrobacter sp. GDP-mannuronate + NADH
-
 ?
GDP-mannose + NAD+ + H2O
-
 Arthrobacter sp. NRRL B1973 GDP-mannuronate + NADH
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 Tris buffer Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arthrobacter sp.