Literature summary for 1.1.1.132 extracted from

Martins, L.O.; Sa-Correia, I.
Alginate biosynthesis in mucoid recombinants of Pseudomonas aeruginosa overproducing GDP-mannose dehydrogenase (1991), Enzyme Microb. Technol., 13, 385-389.

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Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	mucoid strains of cystic fibrosis patients 8821 and its high-alginate-producing spontaneously variant 8821M, enzyme overproduced using 2 different plasmid vectors containing algD under control of the tac promoter mobilized into both strains	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	increase of activity has only limited effect on the stimulation of alginate synthesis, existance of other rate-limiting enzymatic step suggested	Pseudomonas aeruginosa
0.0058	-	+/-0.002, 8821/pMMB24, not IPTG induced	Pseudomonas aeruginosa
0.0128	-	+/-0.0018, 8821/pVD211, not IPTG induced	Pseudomonas aeruginosa
0.0226	-	+/-0.0025, 8821/pVD211, induced by 0.5 mM IPTG	Pseudomonas aeruginosa
0.0265	-	+/-0.003, 8821/pVD211, induced by 3 mM IPTG	Pseudomonas aeruginosa
0.028	-	+/-0.0029, 8821M/pMMB24, not IPTG induced	Pseudomonas aeruginosa
0.0579	-	+/-0.0029, 8821/pVD211, not IPTG induced	Pseudomonas aeruginosa
0.084	-	+/-0.0019, 8821/pVD211, induced by 0.5 mM IPTG	Pseudomonas aeruginosa
0.0942	-	+/-0.004, 8821/pVD211, induced by 3 mM IPTG	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-mannose + NAD+ + H2O	-	Pseudomonas aeruginosa	GDP-mannuronate + NADH	-	ir

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)