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(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
?
-
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-galactonate
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-D-galactonate
pyruvate + D-glyceraldehyde
-
-
-
-
?
2-dehydro-3-deoxy-D-galactonate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
?
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + ?
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
?
-
-
-
-
?
2-dehydro-4-hydroxyoctonate
?
-
-
-
-
?
2-keto-3-deoxy-6-phosphogalactonate
?
-
-
-
-
?
2-oxobutyrate + 2-pyridine carboxaldehyde
?
weak substrate for wild-type enzyme, good substrate for K133Q/T161K mutant
-
?
2-oxobutyrate + D-glyceraldehyde
?
-
weak substrate
-
?
2-pyridinecarboxyaldehyde + pyruvate
(S)-4-hydroxy-2-keto-4-(2'-pyridyl)butyrate
-
typical isolated yield of product is 80-90% after reverse-phase C18 purification. Reaction is also doable with substrates other than 2-pyridinecarboxyaldehyde. Enantiomeric excess greater than 99.7%
-
-
?
3-fluoropyruvate + 2-pyridine carboxaldehyde
?
weak substrate for wild-type enzyme, good substrate for K133Q/T161K mutant
-
?
3-fluoropyruvate + D-glyceraldehyde
?
-
weak substrate
-
?
4-hydroxy-2-oxooctonate
?
-
-
-
-
r
D-2-dehydro-3-deoxygluconate
pyruvate + D-glyceraldehyde
-
poor substrate
-
?
pyruvate + 2-pyridine carboxaldehyde
?
pyruvate + 3-pyridine carboxaldehyde
?
-
wild-type, 0.5% of activity with D-glyceraldehyde 3-phosphate, mutant T161S/S184L, 1% of wild-type activity with D-glyceraldehyde 3-phosphate
-
-
r
pyruvate + 4-pyridine carboxaldehyde
?
-
wild-type, 3% of activity with D-glyceraldehyde 3-phosphate, mutant T161S/S184L, 2.5% of wild-type activity with D-glyceraldehyde 3-phosphate
-
-
r
pyruvate + benzaldehyde
?
-
-
?
Pyruvate + D-erythrose 4-phosphate
3-Deoxy-D-riboheptulosonic acid 7-phosphate
-
28% of the activity with D-glyceraldehyde 3-phosphate
-
?
pyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-gluconate
Pyruvate + D-glyceraldehyde
?
-
35% of the activity with D-glyceraldehyde 3-phosphate
-
-
?
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate
Pyruvate + D-glyceraldehyde 3-phosphate
6-Phospho-2-dehydro-3-deoxy-D-gluconate
pyruvate + D-lactaldehyde
?
-
-
-
?
pyruvate + D-threose
?
-
weak substrate
-
?
pyruvate + DL-3-fluoro-2-hydroxypropanal
?
-
-
-
?
pyruvate + DL-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-D-gluconate
-
-
-
?
pyruvate + glycoaldehyde
?
pyruvate + glyoxylate
(4S)-4-hydroxy-2-oxoglutarate
-
-
-
r
Pyruvate + glyoxylate
?
-
-
-
?
Pyruvate + glyoxylic acid
?
-
93% of the activity with D-glyceraldehyde 3-phosphate
-
-
?
pyruvate + L-erythrose
?
-
weak substrate
-
?
pyruvate + L-glyceraldehyde 3-phosphate
?
-
weak substrate
-
?
pyruvate + L-threose
?
-
weak substrate
-
?
additional information
?
-
2-dehydro-3-deoxy-6-phospho-D-galactonate
?
low activity
-
-
?
2-dehydro-3-deoxy-6-phospho-D-galactonate
?
low activity
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
100% activity
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
100% activity
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
preferred substrate
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
-
-
-
-
r
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
formation of Schiff base intermediate
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
key enzyme in Entner-Doudoroff pathway
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
highly specific in direction of cleavage
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
formation of Schiff base intermediate
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
cleavage reaction is favoured
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
?
pyruvate + 2-pyridine carboxaldehyde
?
-
wild-type, 25% of activity with D-glyceraldehyde 3-phosphate, mutant T161S/S184L, 89% of wild-type activity with D-glyceraldehyde 3-phosphate
-
-
r
pyruvate + 2-pyridine carboxaldehyde
?
-
weak substrate
-
?
pyruvate + D-erythrose
?
weak substrate
-
?
pyruvate + D-erythrose
?
-
weak substrate
-
?
pyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-gluconate
-
-
?
pyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-gluconate
-
wild-type, 4% of activity with D-glyceraldehyde 3-phosphate, mutant T161S/S184L, 1% of wild-type activity with D-glyceraldehyde 3-phosphate
-
-
r
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
-
?
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
-
r
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
-
r
Pyruvate + D-glyceraldehyde 3-phosphate
6-Phospho-2-dehydro-3-deoxy-D-gluconate
-
mutant T161S/S184L, 15% of wild-type activity
-
-
r
Pyruvate + D-glyceraldehyde 3-phosphate
6-Phospho-2-dehydro-3-deoxy-D-gluconate
-
-
-
?
Pyruvate + D-glyceraldehyde 3-phosphate
6-Phospho-2-dehydro-3-deoxy-D-gluconate
-
-
-
?
Pyruvate + D-glyceraldehyde 3-phosphate
6-Phospho-2-dehydro-3-deoxy-D-gluconate
-
-
-
-
?
pyruvate + glycoaldehyde
?
-
-
-
?
pyruvate + glycoaldehyde
?
-
-
-
?
additional information
?
-
-
key enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
2-dehydro-3-deoxy-D-galactonate 6-phosphate is a weak substrate
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
catalyzes exchange of the methyl hydrogen of pyruvate with protons of water
-
-
?
additional information
?
-
-
catalyzes exchange of the methyl hydrogen of pyruvate with protons of water
-
-
?
additional information
?
-
-
constitutive enzyme
-
-
?
additional information
?
-
-
catalyzes exchange of the methyl hydrogen of pyruvate with protons of water
-
-
?
additional information
?
-
-
induced in cells grown on glucose or gluconate
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
catalyzes decarboxylation of oxaloacetate at much slower rate than it cleaves
-
-
?
additional information
?
-
-
catalyzes exchange of the methyl hydrogen of pyruvate with protons of water
-
-
?
additional information
?
-
-
key enzyme in Entner-Doudoroff pathway
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-D-galactonate
pyruvate + D-glyceraldehyde
-
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
2-keto-3-deoxy-6-phosphogalactonate
?
-
-
-
-
?
pyruvate + D-glyceraldehyde 3-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
-
-
?
additional information
?
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
preferred substrate
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-6-phospho-D-gluconate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
-
-
-
-
r
2-dehydro-3-deoxy-D-gluconate
pyruvate + D-glyceraldehyde
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
key enzyme in Entner-Doudoroff pathway
-
r
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
r
additional information
?
-
-
key enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
constitutive enzyme
-
-
?
additional information
?
-
-
induced in cells grown on glucose or gluconate
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
key enzyme in Entner-Doudoroff pathway
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
enzyme of the Entner-Doudoroff pathway
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1.6 - 43
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
0.05 - 1.7
2-dehydro-3-deoxy-6-phospho-D-gluconate
1.5 - 285
2-dehydro-3-deoxy-D-gluconate
0.006 - 1.2
2-dehydro-3-deoxy-D-gluconate 6-phosphate
3 - 32
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
26 - 150
2-dehydro-4-hydroxyoctonate
0.1
2-keto-3-deoxy-6-phosphogluconate
-
-
2 - 146
4-hydroxy-2-oxooctonate
0.038 - 10.5
6-phospho-2-dehydro-3-deoxy-D-gluconate
26.3
benzaldehyde
pH 7.5, K133Q/T161K mutant
38
D-glyceraldehyde 3-phosphate
at pH 7.0 and 42°C
10
pyruvate
pH 7.5, wild-type and K133Q/T161K mutant
1.6
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant T161S/S18L, pH 7.5, 25°C
3.5
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant T161S/S184F, pH 7.5, 25°C
5.7
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant S184F, pH 7.5, 25°C
32
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
wild-type, pH 7.5, 25°C
43
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant T161S, pH 7.5, 25°C
0.05
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant T161S, pH 7.5, 25°C
0.1
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
wild-type, pH 7.5, 25°C
0.24
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant T161S/S18L, pH 7.5, 25°C
0.29
2-dehydro-3-deoxy-6-phospho-D-gluconate
at pH 7.0 and 42°C
0.69
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant T161S/S184F, pH 7.5, 25°C
1.7
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant S184F, pH 7.5, 25°C
1.5
2-dehydro-3-deoxy-D-gluconate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
1.8
2-dehydro-3-deoxy-D-gluconate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
1.9
2-dehydro-3-deoxy-D-gluconate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
2
2-dehydro-3-deoxy-D-gluconate
-
KA3-L1 variant, pH 7.5, 25°C
2.3
2-dehydro-3-deoxy-D-gluconate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
12
2-dehydro-3-deoxy-D-gluconate
-
KA3-L2 variant, pH 7.5, 25°C
13
2-dehydro-3-deoxy-D-gluconate
-
KA3 variant, pH 7.5, 25°C
14
2-dehydro-3-deoxy-D-gluconate
-
KA2 variant, pH 7.5, 25°C
25
2-dehydro-3-deoxy-D-gluconate
-
KA1-2 variant, pH 7.5, 25°C
49
2-dehydro-3-deoxy-D-gluconate
-
KA1-3 variant, pH 7.5, 25°C
55
2-dehydro-3-deoxy-D-gluconate
-
KA1-4 variant, pH 7.5, 25°C
65
2-dehydro-3-deoxy-D-gluconate
-
KA1-1 variant, pH 7.5, 25°C
285
2-dehydro-3-deoxy-D-gluconate
-
wild-type enzyme, pH 7.5, 25°C
0.006
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA3-L1 variant, pH 7.5, 25°C
0.03
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA2 variant, pH 7.5, 25°C
0.03
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA3 variant, pH 7.5, 25°C
0.06
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
pH 7.6, 25°C
0.1
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
0.1
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
wild type enzyme, in 20 mM HEPES, pH 7.5, at 23°C
0.15
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA1-2 variant, pH 7.5, 25°C
0.16
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
0.2
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
-
0.2
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme T161A, in 20 mM HEPES, pH 7.5, at 23°C
0.21
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA1-3 variant, pH 7.5, 25°C
0.22
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
pH 7.5, 40°C
0.3
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme T161V, in 20 mM HEPES, pH 7.5, at 23°C
0.35
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
wild-type enzyme, pH 7.5, 25°C
0.35
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
0.46
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA3-L2 variant, pH 7.5, 25°C
0.9
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
pH 7.5, 70°C
0.95
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
1.2
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
3
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
3
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
3
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
32
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
26
2-dehydro-4-hydroxyoctonate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
51
2-dehydro-4-hydroxyoctonate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
56
2-dehydro-4-hydroxyoctonate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
150
2-dehydro-4-hydroxyoctonate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
2 - 5
4-hydroxy-2-oxooctonate
-
mutant S184F, pH 7.5, 25°C
4.3
4-hydroxy-2-oxooctonate
-
mutant T161S/S18L, pH 7.5, 25°C
15
4-hydroxy-2-oxooctonate
-
mutant T161S, pH 7.5, 25°C
146
4-hydroxy-2-oxooctonate
-
wild-type, pH 7.5, 25°C
0.038
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
0.053
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
0.073
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
0.1
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
0.11
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
0.25
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
10.5
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11 - 240
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
27 - 83
2-dehydro-3-deoxy-6-phospho-D-gluconate
0.016 - 6.08
2-dehydro-3-deoxy-D-gluconate
0.00029 - 284
2-dehydro-3-deoxy-D-gluconate 6-phosphate
6 - 40
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
1.8 - 2.9
2-dehydro-4-hydroxyoctonate
80
2-keto-3-deoxy-6-phosphogluconate
-
-
1.5 - 3.1
4-hydroxy-2-oxooctonate
533
6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
0.000001
benzaldehyde
pH 7.5, K133Q/T161K mutant
0.0000046 - 0.0121
pyruvate
additional information
additional information
-
11
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
wild-type, pH 7.5, 25°C
19
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant S184F, pH 7.5, 25°C
175
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant T161S, pH 7.5, 25°C
212
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant T161S/S184F, pH 7.5, 25°C
240
(4S)-2-oxo-4-hydroxy-4-(2'-pyridyl)butanoate
-
mutant T161S/S18L, pH 7.5, 25°C
27
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant T161S, pH 7.5, 25°C
31
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant T161S/S184F, pH 7.5, 25°C
40
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant S184F, pH 7.5, 25°C
41
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
mutant T161S/S18L, pH 7.5, 25°C
83
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
wild-type, pH 7.5, 25°C
0.016
2-dehydro-3-deoxy-D-gluconate
-
wild-type enzyme, pH 7.5, 25°C
0.019
2-dehydro-3-deoxy-D-gluconate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
0.021
2-dehydro-3-deoxy-D-gluconate
-
KA3-L1 variant, pH 7.5, 25°C
0.04
2-dehydro-3-deoxy-D-gluconate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
0.08
2-dehydro-3-deoxy-D-gluconate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
0.1
2-dehydro-3-deoxy-D-gluconate
-
KA1-1 variant, pH 7.5, 25°C
0.11
2-dehydro-3-deoxy-D-gluconate
-
KA1-4 variant, pH 7.5, 25°C
0.11
2-dehydro-3-deoxy-D-gluconate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
0.13
2-dehydro-3-deoxy-D-gluconate
-
KA1-3 variant, pH 7.5, 25°C
0.16
2-dehydro-3-deoxy-D-gluconate
-
KA1-2 variant, pH 7.5, 25°C
0.43
2-dehydro-3-deoxy-D-gluconate
-
KA3-L2 variant, pH 7.5, 25°C
0.58
2-dehydro-3-deoxy-D-gluconate
-
KA2 variant, pH 7.5, 25°C
1.09
2-dehydro-3-deoxy-D-gluconate
-
KA3 variant, pH 7.5, 25°C
6.08
2-dehydro-3-deoxy-D-gluconate
-
KA2 variant, pH 7.5, 25°C
6.08
2-dehydro-3-deoxy-D-gluconate
-
KA3 variant, pH 7.5, 25°C
0.00029
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
0.0026
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
0.0063
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
0.02
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme T161V, in 20 mM HEPES, pH 7.5, at 23°C
0.0333
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
-
2
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
pH 7.5, 40°C
2.5
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA3 variant, pH 7.5, 25°C
3.2
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
3.9
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
pH 7.5, 70°C
7.8
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA3-L1 variant, pH 7.5, 25°C
9.8
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme T161A, in 20 mM HEPES, pH 7.5, at 23°C
11.2
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA3-L2 variant, pH 7.5, 25°C
52
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA2 variant, pH 7.5, 25°C
74
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
80
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
wild type enzyme, in 20 mM HEPES, pH 7.5, at 23°C
83
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
93
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA1-2 variant, pH 7.5, 25°C
97
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
108
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
KA1-3 variant, pH 7.5, 25°C
284
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
wild-type enzyme, pH 7.5, 25°C
6
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
11
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
27
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
40
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
1.8
2-dehydro-4-hydroxyoctonate
-
mutant enzyme S184D, in 100 mM HEPES, pH 7.5
2
2-dehydro-4-hydroxyoctonate
-
wild type enzyme, in 100 mM HEPES, pH 7.5
2.6
2-dehydro-4-hydroxyoctonate
-
mutant enzyme S184A, in 100 mM HEPES, pH 7.5
2.9
2-dehydro-4-hydroxyoctonate
-
mutant enzyme S184L, in 100 mM HEPES, pH 7.5
1.5
4-hydroxy-2-oxooctonate
-
mutant S184F, pH 7.5, 25°C
1.7
4-hydroxy-2-oxooctonate
-
mutant T161S, pH 7.5, 25°C
2
4-hydroxy-2-oxooctonate
-
wild-type, pH 7.5, 25°C
3.1
4-hydroxy-2-oxooctonate
-
mutant T161S/S18L, pH 7.5, 25°C
0.0000046
pyruvate
pH 7.5, K133Q/T161K mutant
0.0121
pyruvate
pH 7.5, wild-type
additional information
additional information
-
-
-
additional information
additional information
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Meloche, H.P.; Wood, W.A.
Crystallization and characteristics of 2-keto-3-deoxy-6-phosphogluconic aldolase
J. Biol. Chem.
239
3515-3518
1964
Pelomonas saccharophila, Pseudomonas fluorescens
brenda
Meloche, H.P.; Ingram, J.M.; Wood, W.A.
2-Keto-3-deoxy-6-phosphogluconic aldolase (crystalline)
Methods Enzymol.
9
520-524
1966
Pseudomonas fluorescens
-
brenda
Meloche, H.P.
Bromopyruvate inactivation of 2-keto-3-deoxy-6-phosphogluconic aldolase. I. Kinetic evidence for active site specificity
Biochemistry
6
2273-2280
1967
Pelomonas saccharophila, Pelomonas saccharophila A 3.12
brenda
Hammerstedt, R.H.; Mhler, H.; Decker, K.A.; Wood, W.A.
Structure of 2-keto-3-deoxy-6-phosphogluconate aldolase. I. Physical evidence for a three-subunit molecule
J. Biol. Chem.
246
2069-2074
1971
Pseudomonas putida
brenda
Robertson, D.C.; Hammerstedt, R.H.; Wood, W.A.
Structure of 2-keto-3-deoxy-6-phosphogluconate aldolase. II. Chemical evidence for a three-subunit molecule
J. Biol. Chem.
246
2075-2083
1971
Pseudomonas putida
brenda
Vandlen, R.L.; Ersfeld, D.L.; Tulinsky, A.; Wood, W.A.
Confirmation of a trimeric subunit arrangement for 2-keto-3-deoxy-6-phosphogluconic aldolase using X-ray crystallographic methods
J. Biol. Chem.
248
2251-2253
1973
Pseudomonas putida
brenda
Wood, W.A.
2-Keto-3-deoxy-6-phosphogluconic and related aldolases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
281-302
1972
Pseudomonas putida, Pelomonas saccharophila
-
brenda
Hammerstedt, R.H.; Mhler, H.; Decker, K.A.; Ehrsfeld, D.; Wood, W.A.
2-Keto-3-deoxy-6-phosphogluconic aldolase
Methods Enzymol.
42C
258-264
1975
Pseudomonas putida
-
brenda
Meloche, H.P.; Mehler, L.; Wurster, J.M.
The stereochemistry at carbon 3 of pyruvate lyase condensation products. 2-Keto-3-deoxygluconate-6-phosphate aldolase
J. Biol. Chem.
250
6870-6874
1975
Pseudomonas putida
brenda
Meloche, H.P.; Monti, C.T.
The stereochemistry, at carbon 3 of pyruvate lyase condensation products. 2-Keto-3-deoxygluconate-6-phosphate and 2-keto-3-deoxygalactonate-6-phosphate aldolase of Pseudomonas saccharophila
J. Biol. Chem.
250
6875-6879
1975
Pelomonas saccharophila
brenda
Monti, C.T.; Waterbor, J.W.; Meloche, H.P.
Interaction of the chiral pyruvate analog, 2-keto-3-bromobutyrate with pyruvate lyases. 2-Keto-3-deoxygluconate-6-phosphate aldolase of Pseudomonas putida
J. Biol. Chem.
254
5862-5865
1979
Pseudomonas putida
brenda
Suzuki, N.; Wood, W.A.
Complete primary structure of 2-keto-3-deoxy-6-phosphogluconate aldolase
J. Biol. Chem.
255
3427-3435
1980
Pseudomonas putida
brenda
Mavridis, I.M.; Hatada, M.H.; Tulinski, A.; Lebioda, L.
Structure of 2-keto-3-deoxy-6-phosphogluconate aldolase at 2.8 A resolution
J. Mol. Biol.
162
419-444
1982
Pseudomonas putida
brenda
Scopes, R.K.
Use of differential dye-ligand chromatography with affinity elution for enzyme purification: 2-keto-3-deoxy-6-phosphogluconate aldolase from Zymomonas mobilis
Anal. Biochem.
136
525-529
1984
Zymomonas mobilis
brenda
Knappmann, B.R.; Steigel, A.; Kula, M.R.
Investigation of 2-oxo-3-deoxy-5-phosphogluconate aldolase from Zymomonas mobilis as a catalyst in organic synthesis
Biotechnol. Appl. Biochem.
22
107-120
1995
Zymomonas mobilis
-
brenda
Shuttleworth, K.L.; Unz, R.F.; Wichlacz, P.L.
Glucose catabolism in strains of acidophilic, heterotrophic bacteria
Appl. Environ. Microbiol.
50
573-579
1985
Acidiphilium cryptum
brenda
Chakrabarti, S.K.; Mishra, A.K.; Chakrabartty, P.K.
Metabolism of glucose and gluconate in fast- and slow-growing rhizobia
Phytochemistry
26
85-87
1987
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Zymomonas mobilis subsp. mobilis (Q00384), Zymomonas mobilis subsp. mobilis ATCC 31821 (Q00384)
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Identification of 2-keto-3-deoxy-D-gluconate kinase and 2-keto-3-deoxy-D-phosphogluconate aldolase in an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01
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