Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E9K/V118A | KA1-4 variant, improved cleavage of D-2-dehydro-deoxygluconate | Escherichia coli |
G40A/V188A | KA1-1 variant, improved cleavage of D-2-dehydro-deoxygluconate | Escherichia coli |
T84A/I92F | KA1-2 variant, improved cleavage of D-2-dehydro-deoxygluconate | Escherichia coli |
T84A/I92F/V118A | KA2 variant, improved kcat, Km and thermal stability | Escherichia coli |
T84A/I92F/V118A/E138V | KA3 variant, small change of melting temperature | Escherichia coli |
T84A/I92F/V118A/G141S/T105I | KA3-L2 variant, slightly less stable than wild-type | Escherichia coli |
T84A/I92F/V118A/T161A | KA3-L1 variant, enhanced activity towards negatively charged glyoxylate and glyceraldehyde-3-phosphate | Escherichia coli |
V118A | KA1-3 variant, improved cleavage of D-2-dehydro-deoxygluconate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA3-L1 variant, pH 7.5, 25°C | Escherichia coli | |
0.03 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA2 variant, pH 7.5, 25°C | Escherichia coli | |
0.03 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA3 variant, pH 7.5, 25°C | Escherichia coli | |
0.15 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA1-2 variant, pH 7.5, 25°C | Escherichia coli | |
0.21 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA1-3 variant, pH 7.5, 25°C | Escherichia coli | |
0.35 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
0.46 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA3-L2 variant, pH 7.5, 25°C | Escherichia coli | |
2 | - |
2-dehydro-3-deoxy-D-gluconate | KA3-L1 variant, pH 7.5, 25°C | Escherichia coli | |
12 | - |
2-dehydro-3-deoxy-D-gluconate | KA3-L2 variant, pH 7.5, 25°C | Escherichia coli | |
13 | - |
2-dehydro-3-deoxy-D-gluconate | KA3 variant, pH 7.5, 25°C | Escherichia coli | |
14 | - |
2-dehydro-3-deoxy-D-gluconate | KA2 variant, pH 7.5, 25°C | Escherichia coli | |
25 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-2 variant, pH 7.5, 25°C | Escherichia coli | |
49 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-3 variant, pH 7.5, 25°C | Escherichia coli | |
55 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-4 variant, pH 7.5, 25°C | Escherichia coli | |
65 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-1 variant, pH 7.5, 25°C | Escherichia coli | |
285 | - |
2-dehydro-3-deoxy-D-gluconate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate 6-phosphate | Escherichia coli | key enzyme in Entner-Doudoroff pathway | pyruvate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate | - |
Escherichia coli | pyruvate + D-glyceraldehyde | - |
? | |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | - |
Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
r | |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | key enzyme in Entner-Doudoroff pathway | Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
r | |
D-2-dehydro-3-deoxygluconate | poor substrate | Escherichia coli | pyruvate + D-glyceraldehyde | - |
? | |
pyruvate + D-lactaldehyde | - |
Escherichia coli | ? | - |
? | |
pyruvate + DL-3-fluoro-2-hydroxypropanal | - |
Escherichia coli | ? | - |
? | |
pyruvate + DL-glyceraldehyde 3-phosphate | - |
Escherichia coli | 2-dehydro-3-deoxy-D-gluconate | - |
? | |
pyruvate + glycoaldehyde | - |
Escherichia coli | ? | - |
? | |
Pyruvate + glyoxylate | - |
Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
2-dehydro-3-deoxy-D-gluconate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli | |
0.021 | - |
2-dehydro-3-deoxy-D-gluconate | KA3-L1 variant, pH 7.5, 25°C | Escherichia coli | |
0.1 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-1 variant, pH 7.5, 25°C | Escherichia coli | |
0.11 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-4 variant, pH 7.5, 25°C | Escherichia coli | |
0.13 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-3 variant, pH 7.5, 25°C | Escherichia coli | |
0.16 | - |
2-dehydro-3-deoxy-D-gluconate | KA1-2 variant, pH 7.5, 25°C | Escherichia coli | |
0.43 | - |
2-dehydro-3-deoxy-D-gluconate | KA3-L2 variant, pH 7.5, 25°C | Escherichia coli | |
0.58 | - |
2-dehydro-3-deoxy-D-gluconate | KA2 variant, pH 7.5, 25°C | Escherichia coli | |
1.09 | - |
2-dehydro-3-deoxy-D-gluconate | KA3 variant, pH 7.5, 25°C | Escherichia coli | |
2.5 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA3 variant, pH 7.5, 25°C | Escherichia coli | |
6.08 | - |
2-dehydro-3-deoxy-D-gluconate | KA2 variant, pH 7.5, 25°C | Escherichia coli | |
6.08 | - |
2-dehydro-3-deoxy-D-gluconate | KA3 variant, pH 7.5, 25°C | Escherichia coli | |
7.8 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA3-L1 variant, pH 7.5, 25°C | Escherichia coli | |
11.2 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA3-L2 variant, pH 7.5, 25°C | Escherichia coli | |
52 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA2 variant, pH 7.5, 25°C | Escherichia coli | |
93 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA1-2 variant, pH 7.5, 25°C | Escherichia coli | |
108 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | KA1-3 variant, pH 7.5, 25°C | Escherichia coli | |
284 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild-type enzyme, pH 7.5, 25°C | Escherichia coli |