Protein Variants | Comment | Organism |
---|---|---|
T161A | strongly reduced activity | Escherichia coli |
T161V | strongly reduced activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 20 mM HEPES, pH 7.5, at 23°C | Escherichia coli | |
0.2 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme T161A, in 20 mM HEPES, pH 7.5, at 23°C | Escherichia coli | |
0.3 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme T161V, in 20 mM HEPES, pH 7.5, at 23°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-gluconate 6-phosphate | - |
Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
additional information | 2-dehydro-3-deoxy-D-galactonate 6-phosphate is a weak substrate | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxy-6-phosphogluconate aldolase | - |
Escherichia coli |
KDPG aldolase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme T161V, in 20 mM HEPES, pH 7.5, at 23°C | Escherichia coli | |
9.8 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme T161A, in 20 mM HEPES, pH 7.5, at 23°C | Escherichia coli | |
80 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 20 mM HEPES, pH 7.5, at 23°C | Escherichia coli |