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Literature summary for 4.1.2.14 extracted from

  • Selby, C.P.; Sancar, A.
    A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity (2006), Proc. Natl. Acad. Sci. USA, 103, 17696-17700.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged KDPG aldolase is cloned in Escherichia coli BL21 Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
2-keto-3-deoxy-6-phosphogluconate
-
Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified by using Ni2+ affinity column Thermotoga maritima

Storage Stability

Storage Stability Organism
protein can be stored at 4°C for up to 6 months without a significant loss in activity. Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
Thermotoga maritima pyruvate + D-glyceraldehyde 3-phosphate
-
?
2-pyridinecarboxyaldehyde + pyruvate typical isolated yield of product is 80-90% after reverse-phase C18 purification. Reaction is also doable with substrates other than 2-pyridinecarboxyaldehyde. Enantiomeric excess greater than 99.7% Thermotoga maritima (S)-4-hydroxy-2-keto-4-(2'-pyridyl)butyrate
-
?

Synonyms

Synonyms Comment Organism
2-keto-3-deoxy-6-phosphogluconate aldolase
-
Thermotoga maritima
KDPG aldolase
-
Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
80
-
2-keto-3-deoxy-6-phosphogluconate
-
Thermotoga maritima