Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.14 extracted from

  • Zhang, Q.; Gao, F.; Qi, J.; Cheng, H.; Liu, Y.; Gao, G.F.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Streptococcus suis serotype 2 (2008), Acta Crystallogr. Sect. F, 64, 997-999.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine 2-keto-3-deoxy-6-phosphogluconate aldolase is an attractive target for drug therapy in the case of human Streptococcus suis infections Streptococcus suis

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET21a for expression in Escherichia coli BL21DE3 cells Streptococcus suis

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure of 2-keto-3-deoxy-6-phosphogluconate aldolase is solved at a resolution of 2.8 A Streptococcus suis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22920
-
calculated Streptococcus suis
23000
-
determined by SDS-PAGE Streptococcus suis
60000
-
determined by gel filtration, trimer Streptococcus suis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate 6-phosphate Streptococcus suis
-
pyruvate + D-glyceraldehyde 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Streptococcus suis A4VVI7 serotype 2
-

Purification (Commentary)

Purification (Comment) Organism
using a nickel-affinity and a Superdex 200 column Streptococcus suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
Streptococcus suis pyruvate + D-glyceraldehyde 3-phosphate
-
r

Subunits

Subunits Comment Organism
homotrimer
-
Streptococcus suis

Synonyms

Synonyms Comment Organism
2-keto-3-deoxy-6-phosphogluconate aldolase
-
Streptococcus suis
KDPG aldolase
-
Streptococcus suis