Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli K-12 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
S184A | the mutation decreases the efficiency of KDPG retro-aldol cleavage only modestly | Escherichia coli |
S184D | the mutation leads to strongly reduced enzymatic activity | Escherichia coli |
S184L | the mutation is located in the substrate-binding pocket, interacts with the phosphate moiety of KDPG, and improves the catalytic efficiency for the synthesis of a precursor for nikkomycin by 40fold | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.16 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.35 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.95 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
1.2 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
1.5 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
1.8 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
1.9 | - |
2-dehydro-3-deoxy-D-gluconate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
2.3 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
3 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
3 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
3 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
26 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
32 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
51 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
56 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
150 | - |
2-dehydro-4-hydroxyoctonate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-charged chelating Sepharose Fast Flow column chromatography | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, HEPES (100 mM, pH 7.5), NaCl (100 mM), 10% glycerol, at concentrations ranging from 1 to 16 mM, up to 1 year, remains stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-galactonate 6-phosphate | - |
Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
2-dehydro-3-deoxy-D-gluconate | - |
Escherichia coli | ? | - |
? | |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | - |
Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | - |
Escherichia coli | ? | - |
? | |
2-dehydro-4-hydroxyoctonate | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxy-6-phosphogluconate aldolase | - |
Escherichia coli |
KDPG aldolase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.0026 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.0063 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.019 | - |
2-dehydro-3-deoxy-D-gluconate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.04 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.08 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
0.11 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
1.8 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
2 | - |
2-dehydro-4-hydroxyoctonate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
2.6 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
2.9 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
3.2 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
6 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
11 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
27 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
40 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
74 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
83 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
97 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli |