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Literature summary for 4.1.2.14 extracted from

  • Pauluhn, A.; Ahmed, H.; Lorentzen, E.; Buchinger, S.; Schomburg, D.; Siebers, B.; Pohl, E.
    Crystal structure and stereochemical studies of KD(P)G aldolase from Thermoproteus tenax (2008), Proteins, 72, 35-43.
    View publication on PubMed

Application

Application Comment Organism
synthesis stereospecific formation of carbon-carbon bonds is one of the major interests in organic synthetic chemistry, aldolases are part of the most important group of asymmetric C-C-bonding enzymes Saccharolobus solfataricus
synthesis stereospecific formation of carbon-carbon bonds is one of the major interests in organic synthetic chemistry, aldolases are part of the most important group of asymmetric C-C-bonding enzymes Thermoproteus tenax

Cloned(Commentary)

Cloned (Comment) Organism
using the pET expression system Saccharolobus solfataricus
using the pET expression system Thermoproteus tenax

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of the apoprotein is determined to a resolution of 2.0 A, the structure of the protein covalently linked to pyruvate is determined to 2.2 A Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
-
Thermoproteus tenax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-6-phospho-D-gluconate Saccharolobus solfataricus
-
pyruvate + D-glyceraldehyde 3-phosphate
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate Thermoproteus tenax
-
pyruvate + D-glyceraldehyde 3-phosphate
-
r
2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus
-
pyruvate + D-glyceraldehyde
-
?
2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus
-
pyruvate + D-glyceraldehyde
-
r
2-dehydro-3-deoxy-D-gluconate Thermoproteus tenax
-
pyruvate + D-glyceraldehyde
-
r
2-keto-3-deoxy-6-phosphogalactonate Saccharolobus solfataricus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Thermoproteus tenax Q704D1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus
-
Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
r
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
Thermoproteus tenax pyruvate + D-glyceraldehyde 3-phosphate
-
r
2-dehydro-3-deoxy-D-galactonate
-
Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
?
2-dehydro-3-deoxy-D-gluconate
-
Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
r
2-dehydro-3-deoxy-D-gluconate
-
Thermoproteus tenax pyruvate + D-glyceraldehyde
-
r
2-keto-3-deoxy-6-phosphogalactonate
-
Saccharolobus solfataricus ?
-
?

Synonyms

Synonyms Comment Organism
2-keto-3-deoxy-(6-phospho)-gluconate aldolase
-
Saccharolobus solfataricus
2-keto-3-deoxy-(6-phospho)-gluconate aldolase
-
Thermoproteus tenax
KD(P)G aldolase
-
Saccharolobus solfataricus
KD(P)G aldolase
-
Thermoproteus tenax