Information on EC 3.4.24.B9 - ADAM9 endopeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.24.B9
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
ADAM9 endopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
proteolytic degradation of proteins
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
252565-22-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Irish Glen of Imaal terrier
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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silencing of ADAM-9 in melanoma cells significantly reduces cell adhesion to fibroblasts. Ablation of ADAM-9 in fibroblasts almost completely abolishes these cellular interactions and melanoma cell invasion in vitro
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-methoxycoumarin-4-yl)acetyl-PLAQV-dansylphenyl-dinitrophenyl-RSSSR-NH2 + H2O
(7-methoxycoumarin-4-yl)acetyl-PLAQV-dansylphenol + dinitrophenyl-RSSSR-NH2
show the reaction diagram
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quenched fluorescent substrate
-
?
ADAM10 + H2O
?
show the reaction diagram
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shedding of the ectodomain of ADAM10
-
-
?
amyloid precursor protein + H2O
90-100 kDa non-amyloid N-terminal protein fragment
show the reaction diagram
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alpha-secretase activity
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?
amyloid precursor protein + H2O
?
show the reaction diagram
beta-casein + H2O
?
show the reaction diagram
EVHHQKLVFFAE + H2O
EVHH + QKLVFFAE
show the reaction diagram
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peptides derived from beta-amyloid precursor protein, comprises the predicted cleavage site
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?
Fibronectin + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
heparin-binding EGF-like growth factor + H2O
heparin-binding EGF-like growth factor fragment
show the reaction diagram
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cooperative action with bound protein kinase C, membrane-anchored substrate, ectodomain shedding
soluble product
?
insulin B chain + H2O
3 peptides
show the reaction diagram
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cleavage sites are Tyr16-Leu17 and Tyr26-Thr27
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?
insulin B chain + H2O
?
show the reaction diagram
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-
-
-
?
Laminin + H2O
?
show the reaction diagram
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substrate of secreted form of enzyme
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-
?
Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 + H2O
?
show the reaction diagram
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-
-
-
?
pro-heparin-binding EGF-like growth factor + H2O
?
show the reaction diagram
proteins + H2O
peptides
show the reaction diagram
SMAPGAVHLPQP + H2O
SMAPGAVH + LPQP
show the reaction diagram
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peptides derived from P75 TNF-receptor, comprises the predicted cleavage site
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?
SPLAQAVRSSSR + H2O
SPLA + QAVRSSSR
show the reaction diagram
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peptides derived from TNF-alpha, comprises the predicted cleavage site
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?
SPLAQAVRSSSR + H2O
SPLAQAVRS + SSR
show the reaction diagram
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peptides derived from TNF-alpha, comprises the predicted cleavage site
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?
transmembrane collagen XVII + H2O
soluble collagen
show the reaction diagram
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-
-
?
transmembrane collagen XVII + H2O
soluble collagen ectodomain
show the reaction diagram
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substrate protein is an endothelial adhesion molecule on the surface of keratinocytes, protein is shed from the cell surface
cells show altered motility
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
amyloid precursor protein + H2O
?
show the reaction diagram
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enzyme shows alpha-secretase activity, the alpha-secretase might be composed of several ADAM enzymes in a complexe
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?
heparin-binding EGF-like growth factor + H2O
heparin-binding EGF-like growth factor fragment
show the reaction diagram
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cooperative action with bound protein kinase C, membrane-anchored substrate, ectodomain shedding
soluble product
?
pro-heparin-binding EGF-like growth factor + H2O
?
show the reaction diagram
-
cooperative action with bound protein kinase C, membrane-anchored substrate, ectodomain shedding
-
?
proteins + H2O
peptides
show the reaction diagram
transmembrane collagen XVII + H2O
soluble collagen ectodomain
show the reaction diagram
-
substrate protein is an endothelial adhesion molecule on the surface of keratinocytes, protein is shed from the cell surface
cells show altered motility
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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metalloprotease
Zn2+
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mealloprotease
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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Aprotinin
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11% inhibition at 0.025 mM
batimastat
benzamidine
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14% inhibition at 1 mM
brefeldin A
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blocks processing of the enzyme precursor by blocking the vesicle budding in the endoplasmic reticulum
CGS 27023
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coban
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complete blockage of enzyme processing at 0.025 mg/ml
EDTA
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complete inhibition
Hydroxamates
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metalloprotease inhibitors, derivatives FN-439, BB3103, BB 3241, and IC-3
marimastat
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metalloprotease inhibitor SI-27
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o-phenanthroline
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91% inhibition at 0.1 mM
phosphoramidon
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4% inhibition at 0.01 mM
PKVCGYLK
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inhibition less tan 20% at 0.1 mM
PLKCGVSN
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inhibition less tan 20% at 0.1 mM
PLRCGVSN
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inhibition less tan 20% at 0.1 mM
QGGCADHS
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inhibition less tan 20% at 0.1 mM
QGLCGSQH
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inhibition less tan 20% at 0.1 mM
TIMP-1
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metalloprotease inhibitor, 11% inhibition at 0.0005 mM
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TIMP-3
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metalloprotease inhibitor, 15% inhibition at 0.00015 mM
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TNF-alpha protease inhibitor
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additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Phorbol esters
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phorbol myristate acetate ester
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Protein kinase C
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stanolone
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upregulation, 1.5fold increase in activity at 0.000001 mM, 1.7fold at 0.00001 mM
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000014
batimastat
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pH 7.5
0.000054
CGS 27023
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pH 7.5
0.00027
marimastat
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pH 7.5
0.004
PKVCGYLK
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pH 7.5
0.037
PLKCGVSN
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pH 7.5
0.01
PLRCGVSN
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pH 7.5
0.15
QGGCADHS
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pH 7.5
0.15
QGLCGSQH
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pH 7.5
0.000017
TNF-alpha protease inhibitor
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pH 7.5
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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androgen-dependent prostate cancer cell line
Manually annotated by BRENDA team
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lymphoblastoid cell
Manually annotated by BRENDA team
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carotid and femoral arteries, only weak ADAM-9 expression from thyroid artery without atherosclerosis
Manually annotated by BRENDA team
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prostate cancer cell line
Manually annotated by BRENDA team
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grade 3, CIN lesions are premalignant changes
Manually annotated by BRENDA team
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androgen-independent prostate cancer cell line
Manually annotated by BRENDA team
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lung cancer cell line, enzyme mRNA levels are significantly higher in highly brain-metastatic sublines than in the parent or highly bone-metastatic sublines
Manually annotated by BRENDA team
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endothelium cell line
Manually annotated by BRENDA team
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adenocarcinoma cell line
Manually annotated by BRENDA team
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squamous and glandular epithelium of the cervix
Manually annotated by BRENDA team
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synovial membrane-like interface tissue is collected from the proximal bone-cement or bone-stem interfaces around aseptically loosened femoral stems
Manually annotated by BRENDA team
expression of full length and short enzyme form
Manually annotated by BRENDA team
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cells secrete a alternatively spliced variant of enzyme promoting carcinoma invasion
Manually annotated by BRENDA team
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lymphoblastoid cell
Manually annotated by BRENDA team
expression of full length and short enzyme form, with higher level of the short form than in other tissues
Manually annotated by BRENDA team
expression of full length and short enzyme form
Manually annotated by BRENDA team
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in advanced human atherosclerotic plaques of aorta and carotid and femoral arteries, high expression level
Manually annotated by BRENDA team
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ADAM-9 is expressed in human melanoma at the tumor-stroma border where direct or indirect interactions between tumor cells and fibroblasts occur
Manually annotated by BRENDA team
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stromal liver myofibroblast, enzyme expression particularly by cells localized within the tumor stroma at the invasive front
Manually annotated by BRENDA team
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recombinant enzyme promotes a fivefold increase in interleukin IL-6, but not IL-1beta mRNA. Enzyme binds directly to alphabeta5 integrin
Manually annotated by BRENDA team
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androgen-independent prostate cancer cell line
Manually annotated by BRENDA team
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cell culture
Manually annotated by BRENDA team
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elevated level of enzyme in malignant compared to benign tissue. Increase in enzyme protein and mRNA upon exposure of prostate cells to stress conditions. Inhibition of stress-induction of enzyme by actinomycin D and cycloheximide
Manually annotated by BRENDA team
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myeloma cell
Manually annotated by BRENDA team
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osteosarcoma cell line
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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chondrocyte cell line
Manually annotated by BRENDA team
expression of full length and short enzyme form
Manually annotated by BRENDA team
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myeloma cell
Manually annotated by BRENDA team
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in normal epithelium, CIN3 lesions, and squamous cell carcinomas
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12000
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x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE
50000
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SDS-PAGE
68000
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x * 68000, secreted form of ADAM9, SDS-PAGE
79000
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x * 79000, processed mature enzyme, SDS-PAGE, x * 110000, enzyme proform, SDS-PAGE
110000
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x * 79000, processed mature enzyme, SDS-PAGE, x * 110000, enzyme proform, SDS-PAGE
115000
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pro-form, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant secreted metalloprotease domain from Pichia pastoris
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recombinant soluble enzyme from COS-7 cells
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recombinant wild-type and mutant from COS-7 cells
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ADAM-9 quantitative real-time PCR expression analysis
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ADAM9 gene, DNA and amino acid sequence determination and analysis, screening and analysis of the promoter region, expression of mutants iin SH-SY5Y and HeLa cells
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DNA sequence determination and analysis, expression of the short enzyme form, lacking the transmembrane and the cytoplasmic domains due to a deletion in the EGF-like domain, in COS cells, coexpression of amyloid precursor protein
expressed in COS cells
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expressed in EBNA cells
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expression in COS-7 cells and in human glioblastoma A172 cells
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expression of FLAG-tagged enzyme in COS-7 cells
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expression of GST-enzyme disintegrin domain fusion protein Escherichia coli strain BL21
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expression of GST-fusion cytoplasmic enzyme domain in a two-hybrid system in Saccharomyces cerevisiae strain HF7c
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expression of GST-linked cytoplasmic region in Escherichia coli, coeexpression of the enzyme and protein kinase C in COS-7 cells, overexpression of wild-type and deletion mutants in Vero-H cells
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expression of Sec-tagged wild-type and metalloprotease domain deletion mutant in COS-7 cells
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expression of soluble enzyme form, nucleotides 1-1253, with a Myc-epitope in COS-7 cells
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expression of the metalloprotease domain in Pichia pastoris, processing and secretion to the medium
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gene ADAM9, DNA and amino acid sequence determination and analysis, located on chromosome 16, associated with the gPRA candidate locus for generalized progressive retinal atrophy, a group of inherited retinal diseases which are associated with gradual vision loss in various dog breeds, determined by genome-wide homozygosity mapping using SNP arrays and fine mapping of candidate regions. Quantitative real-time RT-PCR expression ADAM9 analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
3 h exposure to 1 mM H2O2 induces ADAM9 expression and ADAM-specific metalloprotease activity in A549 cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine