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Literature summary for 3.4.24.B9 extracted from
- Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin (2001), Protein Expr. Purif., 21, 65-70.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the metalloprotease domain in Pichia pastoris, processing and secretion to the medium | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| batimastat | complete inhibition | Homo sapiens |  |
| EDTA | complete inhibition | Homo sapiens | |
| additional information | no inhibition by the metalloprotease inhibitors TIMP-1, -2, and -4 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | mealloprotease | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
x * 84000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
| proteolytic modification | cleavage by furin-like endopeptidase, also the recombinant enzyme is cleaved in Pichia pastoris | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant secreted metalloprotease domain from Pichia pastoris | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| BM-1604 cell | prostate cancer cell line | Homo sapiens | - |
| ECV-304 cell | endothelium cell line | Homo sapiens | - |
| EFO-27 cell | adenocarcinoma cell line | Homo sapiens | - |
| SAOS-2 cell | osteosarcoma cell line | Homo sapiens | - |
| SW-1353 cell | chondrocyte cell line | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-casein + H2O | recombinant metalloprotease domain is catalytically active against beta-casein | Homo sapiens | ? | - |
? | |
| Fibronectin + H2O | recombinant metalloprotease domain is catalytically active against fibronectin | Homo sapiens | ? | - |
? | |
| Gelatin + H2O | recombinant metalloprotease domain is catalytically active gelatin | Homo sapiens | ? | - |
? | |
| Mca-PLAQAV-Dpa-RSSSR-NH2 + H2O | quenched fluorescent substrate | Homo sapiens | Mca-PLAQA + V-Dpa-RSSSR-NH2 | - |
? | |
| proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| a disintegrin and metalloproteinase domain 9 | - |
Homo sapiens |
| ADAM9 | - |
Homo sapiens |
| M12.209 | Merops-ID | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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