Information on EC 3.4.17.1 - carboxypeptidase A

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.17.1
-
RECOMMENDED NAME
GeneOntology No.
carboxypeptidase A
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
11075-17-5
-
9031-98-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Cephalopina titillator
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
banana weevil
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
dogfish
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
C57B6 mice
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Manually annotated by BRENDA team
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1004067 A+, 1011852 A+, 1013135 A+, 1025514 A+, 1115557 A+, 1139541 A+, 1205212 A+, 1230785 A+, 1261203 A+, 1261376 A+, 1263459 A+, 1264621 A+, 1268955 A+, 1269922 A+, 1270408 A+, 1401265 A+, 1401270 A+, 1401278 A+, 1401305 A+, 1401307 A+, 1401318 A+, 1401345 A+, 1401347 A+, 1401363 A+, 1401400 A+, 1401405 A+, 1401406 A+, 1401410 A+, 1401416 A+, 1401418 A+, 1401420 A+, 1401421 A+, 1401424 A+, 1401428 A+, 1401434 A+, 1401437 A+, 1401439 A+, 1401444 A+, 1401455 A+, 1401487 A+, 1401494 A+, 1401532 A+, 1401533 A+, 1401561 A+, 1401564 A+, 1401573 A+, 1401583 A+, 1401592 A+, 1401596 A+, 1401606 A+, 1401608 A+, 1401610 A+, 1401631 A+, 1401633 A+, 1401656 A+, 1401753 A+, 1401781 A+, 1401800 A+, 1401813 A+, 1401817 A+, 1401836 A+, 1401864 A+, 1401908 A+, 1401954 A+, 1401958 A+, 1402010 A+, 1402024 A+, 1402036 A+, 1402071 A+, 1402077 A+, 1402089 A+, 1402090 A+, 1402164 A+, 1402299 A+, 1402312 A+, 1402314 A+, 1402343 A+, 1402382 A+, 1402418 A+, 1402443 A+, 1402470 A+, 1402472 A+, 1402487 A+, 1402493 A+, 1402522 A+, 1402532 A+, 1402554 A+, 1402569 A+, 1402608 A+, 1402645 A+, 1402678 A+, 1402695 A+, 1402733 A+, 1402736 A+, 1402752 A+, 1402759 A+, 1402768 A+, 1402789 A+, 1402797 A+, 1402812 A+, 1402827 A+, 1402837 A+, 1402847 A+, 1402897 A+, 1402941 A+, 1402946 A+, 1402964 A+, 1402969 A+, 1402981 A+, 1402995 A+, 1402997 A+, 1403006 A+, 1403022 A+, 1403032 A+, 1403057 A+, 1403101 A+, 2107523 A+, 2244394 A+, 2250811 A+, 2343240 A+, 2416106 A+, 2488724 A+, 2526789 A+, 2526836 A+, 2526865 A+, 2526885 A+, 2526938 A+, 2526950 A+, 2526961 A+, 2526982 A+, 2526994 A+, 2527086 A+, 2527096 A+, 2527209 A+, 2527277 A+, 2527302 A+, 2527322 A+, 2527363 A+, 2527409 A+, 2527414 A+, 2527447 A+, 2527488 A+, 2527491 A+, 2527575 A+, 2527603 A+, 2527728 A+, 2527742 A+, 2527744 A+, 2527787 A+, 2527839 A+, 2527867 A+, 2527907 A+, 2527918 A+, 2527941 A+, 2528031 A+, 2528053 A+, 2528062 A+, 2528097 A+, 2528122 A+, 2528146 A+, 2528162 A+, 2528170 A+, 686638 A+, 749758 A+, 780064 A+, 792740 A+, 846293 A+, 913088 A+, 915537 A+, 977590 A+, 984609 A+, 1279149 A++, 1402403 A++, 2148144 A++, 669583 A++, 717909 A++, 822125 A++, 822208 A++, 822219 A++, 1399965 A++, 1401560 A++, 1401792 A++, 2147963 A++, 2148119 A++, 2148135 A++, 2527961 A++, 653991 A++, 668995 A++, 679706 A++, 684017 A++, 745049 A++, 745107 A++, 822222 A++, 822124 A+++
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
preprocarboxypeptidase A1; Japanese flounder, female
SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
protozoa
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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1009028 A+, 1011852 A+, 1012261 A+, 1012551 A+, 1015516 A+, 1016293 A+, 1082758 A+, 1088155 A+, 1136784 A+, 1149291 A+, 1203098 A+, 1260692 A+, 1264795 A+, 1273927 A+, 1384830 A+, 1385266 A+, 1391508 A+, 1401275 A+, 1401297 A+, 1401303 A+, 1401322 A+, 1401324 A+, 1401335 A+, 1401342 A+, 1401352 A+, 1401354 A+, 1401366 A+, 1401376 A+, 1401386 A+, 1401388 A+, 1401395 A+, 1401399 A+, 1401409 A+, 1401412 A+, 1401415 A+, 1401448 A+, 1401449 A+, 1401466 A+, 1401503 A+, 1401529 A+, 1401542 A+, 1401545 A+, 1401580 A+, 1401584 A+, 1401609 A+, 1401617 A+, 1401618 A+, 1401634 A+, 1401639 A+, 1401641 A+, 1401642 A+, 1401646 A+, 1401661 A+, 1401670 A+, 1401671 A+, 1401695 A+, 1401697 A+, 1401698 A+, 1401699 A+, 1401701 A+, 1401703 A+, 1401724 A+, 1401732 A+, 1401740 A+, 1401771 A+, 1401772 A+, 1401774 A+, 1401776 A+, 1401783 A+, 1401788 A+, 1401804 A+, 1401806 A+, 1401809 A+, 1401818 A+, 1401824 A+, 1401826 A+, 1401831 A+, 1401833 A+, 1401836 A+, 1401855 A+, 1401857 A+, 1401872 A+, 1401926 A+, 1401929 A+, 1401931 A+, 1401940 A+, 1401949 A+, 1401963 A+, 1401966 A+, 1401969 A+, 1401970 A+, 1401972 A+, 1401981 A+, 1401990 A+, 1401991 A+, 1401998 A+, 1402016 A+, 1402017 A+, 1402067 A+, 1402079 A+, 1402108 A+, 1402113 A+, 1402120 A+, 1402125 A+, 1402128 A+, 1402136 A+, 1402137 A+, 1402139 A+, 1402146 A+, 1402148 A+, 1402151 A+, 1402154 A+, 1402170 A+, 1402174 A+, 1402180 A+, 1402188 A+, 1402196 A+, 1402216 A+, 1402217 A+, 1402219 A+, 1402243 A+, 1402267 A+, 1402272 A+, 1402273 A+, 1402290 A+, 1402293 A+, 1402331 A+, 1402337 A+, 1402359 A+, 1402364 A+, 1402377 A+, 1402417 A+, 1402444 A+, 1402471 A+, 1402522 A+, 1402523 A+, 1402535 A+, 1402537 A+, 1402552 A+, 1402563 A+, 1402588 A+, 1402595 A+, 1402599 A+, 1402600 A+, 1402602 A+, 1402615 A+, 1402621 A+, 1402628 A+, 1402651 A+, 1402653 A+, 1402665 A+, 1402668 A+, 1402704 A+, 1402708 A+, 1402721 A+, 1402723 A+, 1402755 A+, 1402773 A+, 1402778 A+, 1402784 A+, 1402787 A+, 1402801 A+, 1402842 A+, 1402849 A+, 1402854 A+, 1402933 A+, 1402939 A+, 1402990 A+, 1403015 A+, 1403023 A+, 1403034 A+, 1403035 A+, 1403077 A+, 1403083 A+, 1403087 A+, 2147980 A+, 2184565 A+, 2189260 A+, 2223142 A+, 2223311 A+, 2244328 A+, 2248961 A+, 2249301 A+, 2249914 A+, 2289580 A+, 2455843 A+, 2464509 A+, 2498266 A+, 2503132 A+, 2512733 A+, 2515402 A+, 2526800 A+, 2526808 A+, 2526860 A+, 2526934 A+, 2526935 A+, 2526985 A+, 2527023 A+, 2527026 A+, 2527031 A+, 2527032 A+, 2527039 A+, 2527056 A+, 2527096 A+, 2527116 A+, 2527117 A+, 2527119 A+, 2527145 A+, 2527159 A+, 2527178 A+, 2527232 A+, 2527239 A+, 2527270 A+, 2527291 A+, 2527317 A+, 2527342 A+, 2527346 A+, 2527355 A+, 2527369 A+, 2527403 A+, 2527445 A+, 2527449 A+, 2527457 A+, 2527487 A+, 2527494 A+, 2527496 A+, 2527514 A+, 2527515 A+, 2527534 A+, 2527588 A+, 2527603 A+, 2527624 A+, 2527640 A+, 2527642 A+, 2527727 A+, 2527732 A+, 2527752 A+, 2527760 A+, 2527793 A+, 2527892 A+, 2527912 A+, 2527963 A+, 2528029 A+, 2528094 A+, 2528165 A+, 2528174 A+, 2528227 A+, 2528251 A+, 2528253 A+, 667298 A+, 820299 A+, 821315 A+, 821418 A+, 822112 A+, 822234 A+, 967998 A+, 980182 A+, 1254335 A++, 1264786 A++, 1267455 A++, 1367843 A++, 1401367 A++, 1401470 A++, 1401481 A++, 1401497 A++, 1401515 A++, 1401560 A++, 1401604 A++, 1401619 A++, 1401659 A++, 1401678 A++, 1401907 A++, 2148114 A++, 2148134 A++, 2148136 A++, 2422020 A++, 2519710 A++, 2526874 A++, 668122 A++, 670684 A++, 745049 A++, 785047 A++, 821320 A++, 821336 A++, 821337 A++, 822117 A++, 822188 A++, 846162 A++, 867550 A++, 1399958 A++, 1401795 A++, 1401983 A++, 2106390 A++, 2147958 A++, 2147961 A++, 2148107 A++, 2148117 A++, 2148118 A++, 2148127 A++, 2148132 A++, 2526814 A++, 2527330 A++, 36337 A++, 586913 A++, 669629 A++, 81362 A++, 821490 A++, 822129 A++, 822173 A++, 822186 A++, 822189 A++, 822222 A++, 822127 A+++, 822179 A+++, 36324 A++++, 667459 A++++, 667910 A++++, 821410 A++++, 821436 A++++, 822123 A++++, 822204 A++++
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
rabbitfish
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Manually annotated by BRENDA team
snake
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
toad
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Automatic Mining of ENzyme DAta
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SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
yeasts
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Ala)4 + H2O
(Ala)3 + L-Ala
show the reaction diagram
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-
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ir
(S)-hippuryl-alpha-MePhe + H2O
?
show the reaction diagram
-
-
-
?
(S)-hippuryl-alpha-methylphenyllactic acid + H2O
?
show the reaction diagram
-
-
-
?
(S)-hippuryl-OPhe + H2O
?
show the reaction diagram
-
-
-
?
3-(2-furyl)acryloyl-L-Phe-L-Ala + H2O
3-(2-furyl)acryloyl-L-Phe + L-Ala
show the reaction diagram
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-
-
-
?
3-(2-furyl)acryloyl-L-Phe-L-Ile + H2O
3-(2-furyl)acryloyl-L-Phe + L-Ile
show the reaction diagram
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-
-
-
?
3-(2-furyl)acryloyl-L-Phe-L-Leu + H2O
3-(2-furyl)acryloyl-L-Phe + L-Leu
show the reaction diagram
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-
-
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?
3-(2-furyl)acryloyl-L-Phe-L-Met + H2O
3-(2-furyl)acryloyl-L-Phe + L-Met
show the reaction diagram
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-
-
-
?
3-(2-furyl)acryloyl-L-Phe-L-Phe + H2O
3-(2-furyl)acryloyl-L-Phe + L-Phe
show the reaction diagram
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-
-
-
?
3-(2-furyl)acryloyl-L-Phe-L-Trp + H2O
3-(2-furyl)acryloyl-L-Phe + L-Trp
show the reaction diagram
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-
-
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?
3-(2-furyl)acryloyl-L-Phe-L-Val + H2O
3-(2-furyl)acryloyl-L-Phe + L-Val
show the reaction diagram
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-
-
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?
4-chlorocinnamoyl-L-beta-phenyllactate + H2O
4-chlorocinnamic acid + L-beta-phenyllactate
show the reaction diagram
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-
-
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?
5-dimethyl-aminonaphthalene-1-sulfonyl-Ala-Ala-L-beta-phenyllactate + H2O
5-dimethyl-aminonaphthalene-1-sulfonyl-Ala-Ala + L-beta-phenyllactate
show the reaction diagram
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-
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ir
5-dimethyl-aminonaphthalene-1-sulfonyl-Ala-Ala-Phe + H2O
5-dimethyl-aminonaphthalene-1-sulfonyl-Ala-Ala + L-Phe
show the reaction diagram
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-
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ir
Ac-Phe-ThiaPhe + H2O
Phe + ThiaPhe
show the reaction diagram
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-
-
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?
alpha-Tubulin + H2O
?
show the reaction diagram
Angiotensin I + H2O
?
show the reaction diagram
angiotensin I + H2O
angiotensin II + His-Leu
show the reaction diagram
angiotensin I + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His + Leu
show the reaction diagram
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-
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ir
angiotensin I + H2O
des-Leu10 angiotensin I + Leu
show the reaction diagram
anisylazoformyl-L-Phe + H2O
anisylazoformic acid + L-Phe
show the reaction diagram
-
-
-
?
apoB-100 + H2O
?
show the reaction diagram
apolipoprotein B + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-Gly-L-alpha-hydroxy-beta-phenyllactate + H2O
benzoyl-Gly-Gly-L-alpha-hydroxy-beta-phenyllactate + ?
show the reaction diagram
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-
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ir
benzoyl-Gly-Gly-L-Phe + H2O
benzoyl-Gly-Gly + L-Phe
show the reaction diagram
benzoyl-Gly-L-Phe + H2O
benzoyl-Gly + L-Phe
show the reaction diagram
benzoyl-Gly-phenyllactate + H2O
benzoyl-Gly-phenyllactate + ?
show the reaction diagram
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-
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ir
Benzyloxycarbonyl-Ala-Phe + H2O
Benzyloxycarbonyl-Ala + Phe
show the reaction diagram
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-
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ir
Benzyloxycarbonyl-Gly-Phe + H2O
Benzyloxycarbonyl-Gly + Phe
show the reaction diagram
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-
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ir
benzyloxycarbonyl-Phe-Leu + H2O
benzyloxycarbonyl-Phe + Leu
show the reaction diagram
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-
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ir
beta-actin (169-177) + H2O
?
show the reaction diagram
-
weak substrate
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-
?
big SAAS + H2O
?
show the reaction diagram
-
good substrate
-
-
?
carbobenzoxy-Gly-Gly-L-Tyr + H2O
carbobenzoxy-Gly-Gly + L-Tyr
show the reaction diagram
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-
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ir
carbobenzoxy-Gly-Gly-Leu + H2O
carbobenzoxy-Gly-Gly + Leu
show the reaction diagram
-
-
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ir
carbobenzoxy-Gly-Gly-Phe + H2O
carbobenzoxy-Gly-Gly + Phe
show the reaction diagram
-
-
-
ir
carbobenzoxy-Gly-Gly-Val + H2O
carbobenzoxy-Gly-Gly + Val
show the reaction diagram
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-
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ir
carbobenzoxy-Gly-L-Leu + H2O
carbobenzoxy-Gly + L-Leu
show the reaction diagram
carbobenzoxy-Gly-L-Phe + H2O
carbobenzoxy-Gly + L-Phe
show the reaction diagram
carbobenzoxy-Gly-L-Trp + H2O
carbobenzoxy-Gly + L-Trp
show the reaction diagram
carbobenzyloxy-Gly-hippuryl-L-Phe + H2O
carbobenzyloxy-Gly-hippuric acid + L-Phe
show the reaction diagram
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-
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ir
carboxypeptidase A5 C-terminus + H2O
?
show the reaction diagram
-
requires an overnight incubation for a partial digestion by CPA4
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?
cathepsin D (138-155) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
chromogranin A (374-388) + H2O
?
show the reaction diagram
-
good substrate
-
-
?
chromogranin A (374-390) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
chromogranin B + H2O
?
show the reaction diagram
-
good substrate
-
-
?
cinnamoyl-L-phenyllactate + H2O
cinnamic acid + L-phenyllactate
show the reaction diagram
-
-
-
ir
clathrin light chain A C-terminus + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
dansylglycylglycyl-L-tryptophan + H2O
?
show the reaction diagram
-
assay substrate
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-
?
des-Asp1-angiotensin + H2O
?
show the reaction diagram
-
partially cleaved in 2 h
-
-
?
dynorphin A8 + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
elongation factor 1 beta 2 N-terminus + H2O
?
show the reaction diagram
-
good substrate
-
-
?
endothelin + H2O
?
show the reaction diagram
-
removal of the C-terminal tryptophan
-
-
?
endothelin-1 + H2O
?
show the reaction diagram
-
-
-
-
?
ET-1 + H2O
?
show the reaction diagram
gamma-actin + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
Gly-L-Tyr + H2O
Gly + L-Tyr
show the reaction diagram
glycyl-L-serylglycyl-L-seryl-N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparaginylglycyl-L-seryl-L-tryptophan + H2O
glycyl-L-serylglycyl-L-seryl-N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparaginylglycyl-L-serine + L-tryptophan
show the reaction diagram
-
2,3-diazabicyclo[2.2.2]oct-2-ene-labeled asparagine as a fluorescent amino acid
-
-
?
hippuryl-DL-beta-phenyllactate + H2O
?
show the reaction diagram
-
-
-
?
hippuryl-DL-beta-phenyllactic acid + H2O
hippuric acid + 3-phenyllactic acid
show the reaction diagram
-
-
-
ir
hippuryl-DL-phenylacetic acid + H2O
hippuric acid + DL-phenyllactic acid
show the reaction diagram
-
-
-
ir
hippuryl-DL-phenylalanine + H2O
hippuric acid + DL-phenylalanine
show the reaction diagram
-
-
-
-
?
hippuryl-DL-phenyllactate + H2O
hippuric acid + DL-phenyllactate
show the reaction diagram
hippuryl-DL-phenyllactic acid + H2O
hippuric acid + DL-phenyllactic acid
show the reaction diagram
-
-
-
-
?
Hippuryl-L-Arg + H2O
Hippuric acid + L-Arg
show the reaction diagram
-
-
-
ir
hippuryl-L-Phe + H2O
hippuric acid + L-Phe
show the reaction diagram
hippuryl-L-Phe + H2O
hippuric acid + L-phenylalanine
show the reaction diagram
-
the hydrolysis of the hippuryl-L-Phe molecule by carboxypeptidase A is investigated using both density functional theory and a hybrid quantum mechanical/molecular mechanical approach. The enzymatic reaction is found to proceed via a promoted water pathway with Glu270 serving as the general base and general acid. Free-energy calculations indicate that the first nucleophilic addition step is rate-limiting, with a barrier of 17.9 kcal/mol. Besides activating the zinc-bound water nucleophile, the zinc cofactor also serves as an electrophilic catalyst that stabilizes the substrate carbonyl oxygen during the formation of the tetrahedral intermediate. In the Michaelis complex, Arg127, rather than Zn(II), is responsible for the polarization of the substrate carbonyl and it also serves as the oxyanion hole
-
-
?
hippuryl-L-Phe + H2O
hippuric acid + Phe
show the reaction diagram
hippuryl-L-phenylalanine + H2O
?
show the reaction diagram
-
-
-
-
?
hippuryl-L-phenylalanine + H2O
hippuric acid + L-phenylalanine
show the reaction diagram
hippuryl-Phe + H2O
hippuric acid + Phe
show the reaction diagram
kinetensin + H2O
?
show the reaction diagram
-
-
-
-
?
L-beta-phenyllactate-alpha-((2-naphthoyl)amino)-cinnamoyl ester + H2O
L-beta-phenyllactate + alpha-((2-naphthoyl)amino) cinnamate
show the reaction diagram
-
-
-
ir
L-beta-phenyllactate-alpha-(acetylamino)-cinnamoyl ester + H2O
L-beta-phenyllactate + alpha-(acetylamino)cinnamate
show the reaction diagram
-
-
-
ir
L-beta-phenyllactate-alpha-(benzoylylamino)-cinnamoyl ester + H2O
L-beta-phenyllactate + alpha-(benzoylamino)-cinnamate
show the reaction diagram
-
-
-
ir
Leu-enkephalin + H2O
?
show the reaction diagram
-
partially cleaved in 2 h
-
-
?
Leu5-enkephalin + H2O
?
show the reaction diagram
little SAAS + H2O
?
show the reaction diagram
-
small percentage of cleavage
-
-
?
Met-enkephalin + H2O
?
show the reaction diagram
-
requires an overnight incubation for a partial digestion by CPA4
-
-
?
Met-enkephalin-L-Arg-L-Phe + H2O
Met-enkephalin-L-Arg + L-Phe
show the reaction diagram
-
-
-
-
?
methotrexate-alpha-(1-naphthyl)alanine + H2O
methotrexate + (1-naphthyl)alanine
show the reaction diagram
-
low activity
-
-
?
methotrexate-alpha-phenylalanine + H2O
methotrexate + phenylalanine
show the reaction diagram
-
high activity
-
-
?
methotrexate-phenylalanine + H2O
methotrexate + phenylalanine
show the reaction diagram
N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparaginylglycyl-L-tryptophan + H2O
N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparaginylglycine + L-tryptophan
show the reaction diagram
-
2,3-diazabicyclo[2.2.2]oct-2-ene-labeled asparagine as a fluorescent amino acid
-
-
?
N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparaginylglycylglycyl-L-tryptophan + H2O
N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparaginylglycylglycine + L-tryptophan
show the reaction diagram
-
2,3-diazabicyclo[2.2.2]oct-2-ene-labeled asparagine as a fluorescent amino acid
-
-
?
N-(2-furanacryoyl)-Phe-Phe + H2O
N-(2-furanacryoyl)-Phe + Phe
show the reaction diagram
-
-
-
ir
N-(3-[2-furyl]acryloyl)-L-Phe-L-Phe + H2O
N-(3-[2-furyl]acryloyl)-L-Phe + L-Phe
show the reaction diagram
-
-
-
-
?
N-(3-[2-furyl]acryloyl)-Phe-Phe
?
show the reaction diagram
-
-
-
?
N-(3-[2-furyl]acryloyl)-Phe-Phe + H2O
?
show the reaction diagram
substrate of carboxypeptidase activity assay
-
-
?
N-(3-[2-furyl]acryloyl)-Phe-Phe + H2O
N-(3-[2-furyl]acryloyl)-Phe + Phe
show the reaction diagram
-
-
-
-
?
N-(4-methoxyphenyl-azoformyl)-L-Phe + H2O
?
show the reaction diagram
-
-
-
?
N-(4-methoxyphenylazoformyl)-L-phenylalanine + H2O
?
show the reaction diagram
-
-
-
-
?
N-(4-methoxyphenylazoformyl)-Phe-OH + H2O
?
show the reaction diagram
-
substrate for inhibition assay
-
-
?
N-(methoxyphenyl-azoformyl)-Phe-OH + H2O
?
show the reaction diagram
-
-
-
-
?
N-(trans-3-indoleacryloyl)-L-Phe + H2O
trans-3-indoleacrylate + L-Phe
show the reaction diagram
-
-
-
ir
N-acetyl-Gly-L-phenyllactic acid + H2O
N-acetyl-Gly + L-phenyllactic acid
show the reaction diagram
-
-
-
ir
N-acetyl-L-aspartate
acetate + L-aspartate
show the reaction diagram
-
-
-
-
?
N-acetyl-phenylalanyl-L-3-thiaphenylalanine + H2O
?
show the reaction diagram
-
assay substrate
-
-
?
N-carbobenzoxy-Gly-Gly-L-Leu + H2O
N-carbobenzoxy-Gly-Gly + L-Leu
show the reaction diagram
-
-
-
ir
N-carbobenzoxy-Gly-Gly-L-Phe + H2O
N-carbobenzoxy-Gly-Gly + L-Phe
show the reaction diagram
-
-
-
ir
N-carbobenzoxy-Gly-L-Phe + H2O
N-carbobenzoxy-Gly + L-Phe
show the reaction diagram
-
-
-
ir
N-carbobenzoxy-Gly-L-Tyr + H2O
N-carbobenzoxy-Gly + L-Tyr
show the reaction diagram
-
-
-
ir
N-[3-(2-furyl)acryloyl]-Phe-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
N-[3-(2-furyl)acryloyl]-Phe-Phe + H2O
N-[3-(2-furyl)acryloyl]-Phe + Phe
show the reaction diagram
-
-
-
?
N-[3-(2-furyl)]acryloyl-L-Phe-L-Phe + H2O
N-[3-(2-furyl)]acryloyl-L-Phe + L-Phe
show the reaction diagram
-
-
-
?
N-[4-methoxyphenylazoformyl]-Phe-OH + H2O
?
show the reaction diagram
N2acetyl-N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparaginyl-L-tryptophan + H2O
N2-acetyl-N-(2,3-diazabicyclo[2.2.2]oct-2-en-1-ylmethyl)-L-asparagine + L-tryptophan
show the reaction diagram
-
2,3-diazabicyclo[2.2.2]oct-2-ene-labeled asparagine as a fluorescent amino acid
-
-
?
neuromedin N + H2O
?
show the reaction diagram
neurotensin + H2O
?
show the reaction diagram
O-(trans-4-chlorocinnamoyl)-L-beta-phenyllactate + H2O
trans-4-chlorocinnamic acid + L-phenyllactic acid
show the reaction diagram
-
-
-
-
?
O-(trans-4-chlorocinnamoyl)-L-phenyllactic acid + H2O
trans-4-chlorocinnamic acid + L-phenyllactic acid
show the reaction diagram
-
-
-
-
?
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate + H2O
?
show the reaction diagram
O-(trans-p-chlorocinnamoyl)-L-phenylacetic acid + H2O
?
show the reaction diagram
-
-
-
?
O-(trans-p-chlorocinnamoyl)-L-phenyllactic acid + H2O
?
show the reaction diagram
-
-
-
?
O-(trans-p-chlorocinnamyl)-L-beta-phenyllactate + H2O
?
show the reaction diagram
-
-
-
?
ochratoxin A + H2O
ochratoxin alpha + L-beta-phenylalanine
show the reaction diagram
p-nitrophenyl acetate + H2O
nitrophenolate + acetate
show the reaction diagram
-
-
-
-
?
peptidyl-L-amino acid + H2O
?
show the reaction diagram
-
rapid release: Tyr, Phe, Trp, Leu, Ile, Thr, Gln, His, Ala, Val, homoserine, slow release: Asn, Ser, Lys, MetSO2, very slow release: Gly, Asp, Glu, CysSO3, S-carboxymethylcysteine, not released: Pro, hydroxyproline, Arg, enzyme generally releases C-terminal amino acids, with the exception of C-terminal arginine, lysine and proline
-
-
-
peptidylprolyl isomerase A (118-129) + H2O
?
show the reaction diagram
-
good substrate
-
-
?
peptidylprolyl isomerase A (23-39) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
peptidylprolyl isomerase A (26-39) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
peptidylprolyl isomerase A (84-100) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
peptidylprolyl isomerase A (84-92) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
peroxiredoxin V N-terminus + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Phe-Asn-Arg-Pro-Val + H2O
?
show the reaction diagram
used as substrate in the activity assay
-
-
?
Phe-Asn-Arg-Pro-Val-Asp + H2O
?
show the reaction diagram
used as substrate in the activity assay
-
-
?
Phe-Asn-Arg-Pro-Val-Val + H2O
?
show the reaction diagram
used as substrate in the activity assay
-
-
?
procholecystokinin (46-62) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
procholecystokinin (46-63) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
proenkenphalin octapeptide + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
proenkephalin + H2O
?
show the reaction diagram
-
good substrate
-
-
?
propeptidyl-amidating monooxygenase + H2O
?
show the reaction diagram
-
good substrate
-
-
?
proteasome subunit beta type 6 (34-41) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
protein PEN + H2O
?
show the reaction diagram
-
good substrate
-
-
?
protein PEN-20 + H2O
?
show the reaction diagram
-
good substrate
-
-
?
provasopressin (151-end) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
ribosomal protein S21 C-terminus + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
sarafotoxin + H2O
?
show the reaction diagram
sarafotoxin 6b + H2O
?
show the reaction diagram
-
-
-
-
?
sarafoxin 6b + H2O
?
show the reaction diagram
-
-
-
-
?
secretogranin (287-316) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
secretogranin (300-316) + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
snake venomtoxin + H2O
?
show the reaction diagram
-
-
-
-
?
synaptosomal-associated protein C-terminus + H2O
?
show the reaction diagram
-
good substrate
-
-
?
thioredoxin N-terminus + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
vacuolar ATP synthase subunit 2 C-terminus + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
vasoconstrictive factor endothelin 1 + H2O
?
show the reaction diagram
-
-
-
-
?
voltage-dependent anion channel protein 1 C-terminus + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
xenopsin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Angiotensin I + H2O
?
show the reaction diagram
-
-
-
-
?
apolipoprotein B + H2O
?
show the reaction diagram
-
-
-
-
?
endothelin-1 + H2O
?
show the reaction diagram
-
-
-
-
?
hippuryl-L-phenylalanine + H2O
?
show the reaction diagram
-
-
-
-
?
kinetensin + H2O
?
show the reaction diagram
-
-
-
-
?
methotrexate-phenylalanine + H2O
methotrexate + phenylalanine
show the reaction diagram
-
prodrug activation in SW122 cells
-
-
?
neuromedin N + H2O
?
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
?
peptidyl-L-amino acid + H2O
?
show the reaction diagram
-
rapid release: Tyr, Phe, Trp, Leu, Ile, Thr, Gln, His, Ala, Val, homoserine, slow release: Asn, Ser, Lys, MetSO2, very slow release: Gly, Asp, Glu, CysSO3, S-carboxymethylcysteine, not released: Pro, hydroxyproline, Arg, enzyme generally releases C-terminal amino acids, with the exception of C-terminal arginine, lysine and proline
-
-
-
sarafoxin 6b + H2O
?
show the reaction diagram
-
-
-
-
?
snake venomtoxin + H2O
?
show the reaction diagram
-
-
-
-
?
vasoconstrictive factor endothelin 1 + H2O
?
show the reaction diagram
-
-
-
-
?
xenopsin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-