Literature summary for 3.4.17.1 extracted from

Arolas, J.L.; Popowicz, G.M.; Bronsoms, S.; Aviles, F.X.; Huber, R.; Holak, T.A.; Ventura, S.
Study of a major intermediate in the oxidative folding of leech carboxypeptidase inhibitor: contribution of the fourth disulfide bond (2005), J. Mol. Biol., 352, 961-975.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with leech carboxypeptidase inhibitor, complexing in 50 mM Tris-HCl, pH 7.5, and 100 mM NaCl, at 20°C, complex purification by gel filtration, crystallization by mixing of equal volumes of protein, containing 10-12 mg/ml protein, and reservoir solutions, the latter containing 1.5 M lithium sulfate monohydrate and 100 mM Tris, pH 8.5, sitting drop vapour diffusion method, X-ray diffraction structure determination and analysis at 2.2 A resolution	Bos taurus

Crystallization (Comment)

Organism

enzyme in complex with leech carboxypeptidase inhibitor, complexing in 50 mM Tris-HCl, pH 7.5, and 100 mM NaCl, at 20°C, complex purification by gel filtration, crystallization by mixing of equal volumes of protein, containing 10-12 mg/ml protein, and reservoir solutions, the latter containing 1.5 M lithium sulfate monohydrate and 100 mM Tris, pH 8.5, sitting drop vapour diffusion method, X-ray diffraction structure determination and analysis at 2.2 A resolution

Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
leech carboxypeptidase inhibitor	LCI, tight-binding, competitive inhibition, the inhibitor contains four disulfide bonds, biding structure with CPA, oxidative folding pathway and intermediate of wild-type and mutant C19A/C43A mutant, determination of thermodynamics and conformational stability of wild-type and mutant enzymes at pH 8.4/high concentration of DTT, overview	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Subunits

Subunits	Comment	Organism
More	structure analysis of enzyme in complex with leech carboxypeptidase inhibitor	Bos taurus

Synonyms

Synonyms	Comment	Organism
CPA	-	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)