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Literature summary for 3.4.17.1 extracted from

  • Folk, J.E.; Schirmer, E.W.
    The porcine pancreatic carboxypeptidase A system (1963), J. Biol. Chem., 238, 3884-3894.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Sus scrofa

General Stability

General Stability Organism
little effect of urea on enzyme activity Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Sus scrofa
0.11
-
hippuryl-L-phenyllactate mixture of isoenzyme 2 and 3 Sus scrofa
14.3
-
carbobenzoxy-Gly-L-Trp mixture of isoenzyme 2 and 3 Sus scrofa
16.1
-
carbobenzoxy-Gly-L-Phe mixture of isoenzyme 2 and 3 Sus scrofa
33
-
carbobenzoxy-Gly-L-Leu mixture of isoenzyme 2 and 3 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloenzyme Sus scrofa
Zn2+ 1 mol Zn per mol of enzyme Sus scrofa
Zn2+ 0.91-1.07 mol Zn per mol enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34800
-
sedimentation equilibrium Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
3 electrophoretically distinct forms
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
4°C, ammonium sulfate, pH 6.0, precipitated procarboxypeptidase Sus scrofa
5°C, Tris-HCl, pH 7.5, crystals under toluene atmosphere, indefinite Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbobenzoxy-Gly-L-Leu + H2O
-
Sus scrofa carbobenzoxy-Gly + L-Leu
-
ir
carbobenzoxy-Gly-L-Phe + H2O
-
Sus scrofa carbobenzoxy-Gly + L-Phe
-
ir
carbobenzoxy-Gly-L-Trp + H2O
-
Sus scrofa carbobenzoxy-Gly + L-Trp
-
ir
hippuryl-DL-phenyllactate + H2O
-
Sus scrofa hippuric acid + DL-phenyllactate
-
ir
hippuryl-L-phenyllactate + H2O
-
Sus scrofa hippuric acid + L-phenyllactate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
48% inactivation after 5 min Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29
-
carbobenzoxy-Gly-L-Leu mixture of isoenzyme 2 and 3 Sus scrofa
29.8
-
carbobenzoxy-Gly-L-Trp mixture of isoenzyme 2 and 3 Sus scrofa
139
-
carbobenzoxy-Gly-L-Phe mixture of isoenzyme 2 and 3 Sus scrofa
1000
-
hippuryl-L-phenyllactate mixture of isoenzyme 2 and 3 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11.6 35% activity remaining at pH 4 after 30 min, 87% at pH 11.6 Sus scrofa