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Literature summary for 3.4.17.1 extracted from
- Rat peritoneal mast cell carboxypeptidase: localization, purification, and enzymatic properties (1980), FEBS Lett., 110, 292-296.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Rattus norvegicus |  |
| potato carboxypeptidase inhibitor | - |
Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus | |
| 0.062 | - |
hippuryl-L-phenyllactate | - |
Rattus norvegicus | |
| 0.14 | - |
Benzyloxycarbonyl-Ala-Phe | - |
Rattus norvegicus | |
| 0.23 | - |
Benzyloxycarbonyl-Phe-Leu | - |
Rattus norvegicus | |
| 0.31 | - |
Benzyloxycarbonyl-Gly-Phe | - |
Rattus norvegicus | |
| 0.45 | - |
benzoyl-Gly-L-Phe | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| secretory granule | - |
Rattus norvegicus | 30141 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
in presence of urea and 2-mercaptoethanol, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mast cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.54 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Gly-L-Phe + H2O | - |
Rattus norvegicus | benzoyl-Gly + L-Phe | - |
ir | |
| Benzyloxycarbonyl-Ala-Phe + H2O | - |
Rattus norvegicus | Benzyloxycarbonyl-Ala + Phe | - |
ir | |
| Benzyloxycarbonyl-Gly-Phe + H2O | - |
Rattus norvegicus | Benzyloxycarbonyl-Gly + Phe | - |
ir | |
| benzyloxycarbonyl-Phe-Leu + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Phe + Leu | - |
ir | |
| hippuryl-DL-phenyllactate + H2O | - |
Rattus norvegicus | hippuric acid + DL-phenyllactate | - |
ir | |
| hippuryl-L-phenyllactate + H2O | - |
Rattus norvegicus | hippuric acid + L-phenyllactate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | amino acid composition described | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.2 | - |
Benzyloxycarbonyl-Gly-Phe | - |
Rattus norvegicus | |
| 16 | - |
benzoyl-Gly-L-Phe | - |
Rattus norvegicus | |
| 66 | - |
Benzyloxycarbonyl-Phe-Leu | - |
Rattus norvegicus | |
| 89 | - |
Benzyloxycarbonyl-Ala-Phe | - |
Rattus norvegicus | |
| 454 | - |
hippuryl-L-phenyllactate | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | esterase activity, broad optimum | Rattus norvegicus |
| 8 | - |
peptidase activity, sharp optimum | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | pH 5.5: about 35% of maximum activity, pH 10: about 40% of maximum activity | Rattus norvegicus |
| 6 | 9.5 | pH 6.0, pH 9.5: about 25% of maximum peptidase activity | Rattus norvegicus |
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