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Literature summary for 3.4.17.1 extracted from

  • Harris, J.; Schwinn, N.; Mahoney, J.A.; Lin, H.; Shaw, M.; Howard, C.J.; da Silva, R.P.; Gordon, S.
    A vitellogenic-like carboxypeptidase expressed by human macrophages is localized in endoplasmic reticulum and membrane ruffles (2006), Int. J. Exp. Pathol., 87, 29-39.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information lipopolysaccharide has no effect on expression in monocyte-derived dendritic cells Homo sapiens
phorbol myristate acetate
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET-28a(+) and expressed in Escherichia coli BL21-DE3 cells. BALB/c mice inoculated subcutaneously with recombinant CPVL. CHO cells transiently transfected with CPVL-EGFP expression vector Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
endoglycosidase H lowers CPVL expression in monocyte-derived macrophages Homo sapiens
additional information O-glycosidase has no effect in monocyte-derived macrophages. CPVL in THP-1 and Jurkat cell lines are insensitive to treatment with a cocktail of O-glycosidase, N-glycosidase and endoglycosidase H Homo sapiens
N-glycosidase lowers CPVL expression in monocyte-derived macrophages Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
endoplasmic reticulum
-
Homo sapiens 5783
-
lamellipodium
-
Homo sapiens 30027
-
membrane present in ruffles of macrophages, absent from outer plasma membrane Homo sapiens 16020
-
phagosome CPVL present in early (5 min incubation) latex bead phagosomes, it is not retained in the mature, cathepsin D-positive phagolysosome (60 min incubation) Candida albicans
-
-

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to more than 80% purity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
macrophage in primary macrophages, CPVL is glycosylated with high mannose residues Homo sapiens
-
monocyte CPVL is up-regulated during the maturation of monocytes to macrophages. Expression in monocyte-derived dendritic cells Homo sapiens
-
additional information present in Jurkat T-cell line, is not expressed by monocyte depleted peripheral blood mononuclear cells Homo sapiens
-
THP-1 cell expressed in phorbol myristate acetate-stimulated cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CPVL colocalizes with both macrophage-inhibitory protein-1alpha and tumour necrosis factor-alpha Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
carboxypeptidase vitellogenic-like
-
Homo sapiens
CPVL
-
Homo sapiens
CPVL
-
Candida albicans
vitellogenic-like carboxypeptidase
-
Candida albicans