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Literature summary for 3.4.17.1 extracted from

  • Bayes, A.; Fernandez, D.; Sola, M.; Marrero, A.; Garcia-Pique, S.; Aviles, F.X.; Vendrell, J.; Gomis-Rueth, F.X.
    Caught after the act: a human A-type metallocarboxypeptidase in a product complex with a cleaved hexapeptide (2007), Biochemistry, 46, 6921-6930.
    View publication on PubMed
Search for more literature for 3.4.17.1

Activating Compound

Activating Compound Comment Organism Structure
Trypsin activates zymogen Homo sapiens

Application

Application Comment Organism
medicine engaged in prostate cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
hCPA4 is produced as a zymogen through recombinant heterologous overexpression using vector pPIC9 and the methylotrophic yeast Pichia pastoris Homo sapiens
produced as a zymogen through recombinant heterologous overexpression using vector pPIC9 and the methylotrophic yeast Pichia pastoris as expression host Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
CPA4-hexapeptide complex crystallized by sitting drop vapor diffusion method, to 1.6 A resolution Homo sapiens
structural details of a true cleaved double-product complex with a hexapeptide of human ocarboxypeptidase A4 employing diffraction data to 1.6 A resolution are provided Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Gly-Asn-Arg-Pro-Thr
-
 Homo sapiens
Gly-Asn-Arg-Pro-Val-Thr
-
 Homo sapiens
Phe-Asn-Arg-Ala-Val-Asp
-
 Homo sapiens
Phe-Asn-Arg-Ala-Val-Val
-
 Homo sapiens
Phe-Asn-Arg-Pro-Val
-
 Homo sapiens
Phe-Asn-Arg-Pro-Val-Asp
-
 Homo sapiens
Phe-Asn-Arg-Pro-Val-Val
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UI42
-
-

Purification (Commentary)

Purification (Comment) Organism
by anion-exchange chromatography Homo sapiens
the active protein is obtained through tryptic activation, the resulting protein-product complex is subsequently purified by anion-exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(4-methoxyphenyl-azoformyl)-L-Phe + H2O
-
 Homo sapiens ?
-
 ?
Phe-Asn-Arg-Pro-Val + H2O used as substrate in the activity assay Homo sapiens ?
-
 ?
Phe-Asn-Arg-Pro-Val-Asp + H2O used as substrate in the activity assay Homo sapiens ?
-
 ?
Phe-Asn-Arg-Pro-Val-Val + H2O used as substrate in the activity assay Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
A-type metallocarboxypeptidase
-
 Homo sapiens
carboxypeptidase a
-
 Homo sapiens
carboxypeptidase A4
-
 Homo sapiens
CPA4
-
 Homo sapiens
hCPA4
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.63
-
-
 Homo sapiens Phe-Asn-Arg-Ala-Val-Val
0.71
-
-
 Homo sapiens Phe-Asn-Arg-Pro-Val-Val
0.72
-
-
 Homo sapiens Phe-Asn-Arg-Ala-Val-Asp
0.91
-
-
 Homo sapiens Phe-Asn-Arg-Pro-Val-Asp
2.5
-
-
 Homo sapiens Gly-Asn-Arg-Pro-Thr
4
-
-
 Homo sapiens Phe-Asn-Arg-Pro-Val
5
-
 IC50 4-6 mM Homo sapiens Phe-Asn-Arg-Pro-Val