Information on EC 3.4.14.2 - dipeptidyl-peptidase II

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.4.14.2
-
RECOMMENDED NAME
GeneOntology No.
dipeptidyl-peptidase II
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal dipeptide, Xaa-Yaa-/-, preferentially when Yaa is Ala or Pro. Substrates are oligopeptides, preferentially tripeptides
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
exopeptidase, N-terminus dipeptide
-
CAS REGISTRY NUMBER
COMMENTARY hide
76199-23-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
WM-23
-
-
Manually annotated by BRENDA team
WM-23
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Ala)3 + H2O
?
show the reaction diagram
(Ala)4 + H2O
?
show the reaction diagram
-
-
-
-
?
(Gly-Pro-Ala)n + H2O
Gly-Pro + Ala
show the reaction diagram
-
-
-
?
Ala-Ala-2-naphthylamide + H2O
Ala-Ala + 2-naphthylamine
show the reaction diagram
Ala-Ala-2-nitroanilide + H2O
Ala-Ala + 2-nitroaniline
show the reaction diagram
-
low activity
-
?
Ala-Ala-4-nitroanilide + H2O
Ala-Ala + 4-nitroanilide
show the reaction diagram
Ala-Ala-Ala + H2O
Ala-Ala + Ala
show the reaction diagram
-
54% activity compared to Met-Ala-Ser
-
-
?
Ala-Ala-Ala-Ala + H2O
Ala-Ala + Ala-Ala
show the reaction diagram
-
39% activity compared to Met-Ala-Ser
-
-
?
Ala-Ala-p-nitroanilide + H2O
Ala-Ala + p-nitroaniline
show the reaction diagram
-
481% activity compared to Gly-Pro-7-amido-4-methylcoumarin
-
-
?
Ala-Ala-Phe-OH + H2O
Ala-Ala + Phe-OH
show the reaction diagram
Ala-Phe-4-nitroanailide + H2O
Ala-Phe + 4-nitroaniline
show the reaction diagram
Ala-Pro-2-(4-methoxy)-naphthylamine + H2O
?
show the reaction diagram
Ala-Pro-4-nitroanilide + H2O
Ala-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Ala-Pro-amino-4-trifluoromethylcoumarin + H2O
Ala-Pro + amino-4-trifluoromethylcoumarin
show the reaction diagram
Ala-Pro-p-nitroanilide + H2O
Ala-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Arg-Ala-2-naphthylamide + H2O
Arg-Ala + 2-naphthylamine
show the reaction diagram
Arg-Pro-2-naphthylamide + H2O
Arg-Pro + 2-naphthylamine
show the reaction diagram
-
27% of the activity with Phe-Pro-2-naphthylamide
-
-
?
Arg-Pro-4-nitroanilide + H2O
Arg-Pro + 4-nitroanilide
show the reaction diagram
Arg-Pro-Lys-Pro + H2O
Arg-Pro + Lys-Pro
show the reaction diagram
Arg-Pro-p-nitroanilide + H2O
Arg-Pro + p-nitroaniline
show the reaction diagram
Asn-Pro-p-nitroanilide + H2O
Asn-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Asp-Pro-4-nitroanilide + H2O
Asp-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
bradykinin fragment 1-3 + H2O
?
show the reaction diagram
-
-
-
-
?
bradykinin fragment 1-5 + H2O
?
show the reaction diagram
-
-
-
-
?
casomorphin + H2O
?
show the reaction diagram
Glu-Pro-4-nitroanilide + H2O
Glu-Pro + 4-nitroaniline
show the reaction diagram
Gly-Ala-7-amido-4-methylcoumarin + H2O
Gly-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Gly-Ala-Ala + H2O
Gly-Ala + Ala
show the reaction diagram
-
-
-
-
?
Gly-L-Pro-4-nitroanilide + H2O
Gly-L-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Gly-Pro-2-(4-methoxy)-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Pro-2-naphthylamide + H2O
Gly-Pro + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Gly-Pro-4-methylcoumarin 7-amide + H2O
Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
Gly-Pro-7-amido-4-methylcoumarin + H2O
Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
Gly-Pro-Ala + H2O
Gly-Pro + Ala
show the reaction diagram
Gly-Pro-Gly-Gly + H2O
Gly-Pro + Gly-Gly
show the reaction diagram
-
16% activity compared to Met-Ala-Ser
-
-
?
Gly-Pro-Leu-Gly + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Pro-p-nitroanilide + H2O
Gly-Pro + p-nitroaniline
show the reaction diagram
His-Ala-p-nitroanilide + H2O
His-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
His-Pro-p-nitroanilide + H2O
His-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
His-Pro-Val + H2O
His-Pro + Val
show the reaction diagram
-
-
-
-
?
L-Lys-L-Ala-5-chloro-1-anthraquinonylhydrazide-2HBr + H2O
L-Lys-L-Ala + 5-Cl-1-anthraquinonylhydrazine + HBr
show the reaction diagram
-
i.e. Lys-Ala-CAH, synthetic substrate for colorimetric assay
i.e. CAH, forms a highly insoluble, deeply colored hydrazone when coupled to an aromatic aldehyde, e.g. 4-nitrobenzaldehyde or piperonal, useful for histochemical detection of the enzyme
?
Leu-Ala-2-naphthylamide + H2O
Leu-Ala + 2-naphthylamine
show the reaction diagram
Lys-Ala-2-(4-methoxy)-naphthylamide Lys-Ala-4-methoxy-2-naphthylamide + H2O
?
show the reaction diagram
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
Lys-Ala-4-methylcoumaryl-7-amide + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
Lys-Ala-7-amido-4-methylcoumarin + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
Lys-Ala-Ala + H2O
Lys-Ala + Ala
show the reaction diagram
Lys-Ala-OCH3 + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Ala-p-nitroanilide + H2O
Lys-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Lys-Ala-Pro + H2O
Lys-Ala + Pro
show the reaction diagram
-
-
-
-
?
Lys-Arg-2-naphthylamide + H2O
Lys-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Lys-Pro-2-(4-methoxy)-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Pro-2-(4-methoxy)-naphthylamine + H2O
?
show the reaction diagram
Lys-Pro-2-naphthylamide + H2O
Lys-Pro + 2-naphthylamine
show the reaction diagram
Lys-Pro-4-nitroanilide + H2O
Lys-Pro + 4-nitroaniline
show the reaction diagram
Lys-Pro-7-amido-4-methylcoumarin + H2O
Lys-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Lys-Pro-p-nitroanilide + H2O
Lys-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Met-Ala-Ser + H2O
Met-Ala + Ser
show the reaction diagram
-
100% activity
-
-
?
Met-Met-Ala + H2O
Met-Met + Ala
show the reaction diagram
-
-
-
-
?
Met-Met-Met + H2O
Met-Met + Met
show the reaction diagram
-
-
-
-
?
Nle-Nle-p-nitroanilide + H2O
Nle-Nle + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Nle-Nle-rhodamine + H2O
Nle-Nle + rhodamine
show the reaction diagram
-
-
?
Nle-Pro-7-amido-4-methylcoumarin + H2O
Nle-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Nle-Pro-rhodamine + H2O
Nle-Pro + rhodamine
show the reaction diagram
-
-
?
Phe-Pro-2-naphthylamide + H2O
Phe-Pro + 2-naphthylamine
show the reaction diagram
Phe-Pro-4-methylcoumarin 7-amide + H2O
Phe-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Phe-Pro-Ala + H2O
Phe-Pro + Ala
show the reaction diagram
-
-
-
-
?
Phe-Pro-p-nitroanilide + H2O
Phe-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Pro-Ala-p-nitroanilide + H2O
Pro-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Ser-Met-Gln + H2O
Ser-Met + Gln
show the reaction diagram
-
-
-
-
?
Ser-Met-Glu + H2O
Ser-Met + Glu
show the reaction diagram
-
-
-
-
?
Ser-Met-OCH3 + H2O
?
show the reaction diagram
-
-
-
-
?
Ser-Pro-p-nitroanilide + H2O
Ser-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
Tyr-Ala-p-nitroanilide + H2O
Tyr-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Val-Ala-4-nitroanilide + H2O
Val-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Val-Ala-Phe-OH + H2O
Val-Ala + Phe-OH
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Arg-Pro-Lys-Pro + H2O
Arg-Pro + Lys-Pro
show the reaction diagram
Bradykinin + H2O
?
show the reaction diagram
casomorphin + H2O
?
show the reaction diagram
Substance P + H2O
?
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,S)-(2E)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0361 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2E)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0404 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2Z)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0329 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2Z)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0873 mM, low inhibitory potential against EC 3.4.14.5
(3S)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
-
-
(3S)-4-[(3aR,6aR)-hexahydrocyclopenta[b]pyrrol-1(2H)-yl]-4-oxobutane-1,3-diamine
-
AX8819, potent and selective inhibitor of DPP II
(3S)-N1-(4-chlorobenzyl)-4-(2-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 2.3 mM; IC50: 2.3 nM
(3S)-N1-(4-chlorobenzyl)-4-(3-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
-
0.62 nM; IC50: 0.62 nM
(3S)-N1-(4-chlorobenzyl)-4-(3-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 1.13 nM
(3S)-N1-(4-chlorobenzyl)-4-(4-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 0.39 nM
(3S)-N1-(4-chlorobenzyl)-4-(4-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 0.55 nM
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
(D-Ala)3
-
5 mM, 30% inhibition of hydrolysis of (L-Ala)3, noncompetitive
1-((2S)-2-amino-4-((4-chlorobenzyl)amino)butanoyl)piperidin-3-ol
-
IC50: 54.7 nM
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidin-4-ol
-
IC50: 113 nM
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidine-2-carbonitrile
-
IC50: 6.5 nM
1-([(2-phenylethyl)amino]acetyl)-2-pyrrolidinecarbonitrile
-
0.0163 mM
1-([(4-fluorobenzyl)amino]acetyl)-2-pyrrolidinecarbonitrile
-
IC50: 0.0255 mM
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.00104 mM, low inhibitory potential against EC 3.4.14.5
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)piperidin-4-amine
-
-
1-benzyl-N-(2-cyclopentylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.0013 mM, low inhibitory potential against EC 3.4.14.5
1-benzyl-N-(2-oxo-2-piperidin-1-ylethyl)piperidin-4-amine
-
-
1-benzyl-N-[2-oxo-2-(1-piperidinyl)ethyl]-4-piperidinamine
-
IC50: 0.0031 mM, low inhibitory potential against EC 3.4.14.5
1-[(1-adamantylamino)acetyl]-2-pyrrolidinecarbonitrile
-
IC50: 0.1335 mM
1-[(2S)-2-amino-4-(benzylamino)butanoyl]piperidine-2-carbonitrile
-
IC50: 4.0 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-3-ol
-
IC50: 54.7 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-4-ol
-
IC50: 113 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidine-2-carbonitrile
-
IC50: 6.5 nM
3-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)aminomethyl)-benzonitrile
-
IC50: 2.7 nM
4-(2-aminoethyl)benzenesulfonyl fluoride
4-Chloromercuriphenylsulfonate
4-oxo-4-(1-piperidinyl)-N1 -(4-piperidinyl)-1,3-(S)-butanedi-amine
-
IC50: 85 nM
4-oxo-4-(1-piperidinyl)-N1-(2-thienylmethyl)-1,3-(S)-butanediamine
-
IC50: 0.8 nM
4-oxo-4-(1-piperidinyl)-N1-(4-pyridinylmethyl)-1,3-(S)-butanediamine
-
IC50: 0.75 nM
4-oxo-4-(1-piperidinyl)-N3-(4-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.017 mM
4-oxo-N1-(2-phenylethyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.9 nM
4-oxo-N1-(3-phenylpropyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.91 nM
4-oxo-N1-phenyl-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.0015 mM
6-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)amino)nicotinonitrile
-
IC50: 0.0046 mM
Ala-PSI[CS-N]-pyrrolidine
Ala-PSI[CS-N]-thiazolidine
Ala-pyrrolidine
Ala-thiazolidine
ammonium sulfate
-
-
benzamidine
-
10% inhibition at 1 mM, preincubation at room temperature for 15 min
benzethonium chloride
-
-
benzyl 4-[(2-cyclohexylidene-2-fluoroethyl)amino]-1-piperidinecarboxylate
-
IC50: 0.0128 mM, low inhibitory potential against EC 3.4.14.5
bestatin
Cetyltrimethylammonium bromide
-
-
diaminobutyric acid-piperidide
-
-
diisopropyl fluorophosphate
diisopropylfluorophosphate
-
complete inhibition at 1 mM, 25% inhibition at 0.1 mM, preincubation at room temperature for 15 min
Diprotin A
-
not selective for the enzyme
EDTA
-
-
epostatin
-
-
epsilon-benzoyloxycarbonyl-Lys-Pro
-
1 mM, 31% inhibition of DPP II-S, 41% inhibition of DPP II-M
-
epsilon-Z-Lys-Pro
-
competitive inhibitor
Fe2+
-
significant inhibition
histamine
hyamine 10-X
-
-
Ile cyanothiazolidide
-
not selective for the enzyme
Ile-PSI[CS-N]-pyrrolidine
Ile-PSI[CS-N]-thiazolidine
Ile-pyrrolidine
Ile-thiazolidine
iodoacetate
Lys piperidide
-
not selective for the enzyme
Lys thiazolidide
-
not selective for the enzyme
Lys-Ala chloromethyl ketone
-
0.1 mM, complete inhibition, active-site-directed inhibitor
Lys-Ala-CH2Cl
-
inhibitory at 0.1 mM
lysyl-piperidide
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclohexylidene-2-fluoroethanamine
-
IC50: 0.0129 mM, low inhibitory potential against EC 3.4.14.5
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclopentylideneethanamine
-
0.0075 mM; IC50: 0.0075 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-1-adamantanamine
-
IC50: 0.0913 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.0042 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(2-phenylethyl)-amine
-
IC50: 0.011 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-fluorobenzyl)-amine
-
IC50: 0.0219 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-methoxybenzyl)amine
-
IC50: 0.0134 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)aniline
-
IC50: 0.1 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)cyclohexanamine
-
IC50: 0.5 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-phenylethyl)amine
-
IC50: 0.0272 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-pyridinylethyl)amine
-
IC50: 0.0096 mM, low inhibitory potential against EC 3.4.14.5; IC50: 0.0157 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-fluorobenzyl)amine
-
IC50: 0.0108 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-methoxy-benzyl)amine
-
IC50: 0.0155 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)cyclohexanamine
-
IC50: 0.09 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylideneethyl)cyclohexanamine
-
IC50: 0.062 mM; IC50: 0.062 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
-
N-4(chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrochloride
-
-
N-benzyl-2-cyclohexylidene-2-fluoroethanamine
-
IC50: 0.034 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-cyclopentylidene-2-fluoroethanamine
-
IC50: 0.0378 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-cyclopentylideneethanamine
-
IC50: 0.026 mM; IC50: 0.026 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.397 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.5 mM
N-benzyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.0484 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.177 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.276 mM
N-cyclohexyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.069 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.092 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.052 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.063 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.042 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.067 mM, low inhibitory potential against EC 3.4.14.5
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]acetamide
-
IC50: 0.0015 mM
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]guanidine bis-(trifluoroacetate)
-
IC50: 0.0018 mM
N-[3-amino-1-(S)-(1-piperidinylcarbonyl)propyl]guanidine bis-(trifluoroacetate)
-
IC50: 0.0074 mM
N-[3-amino-4-oxo-4-(1-piperidinyl)butyl]benzamide
-
IC50: 0.0074 mM
N1 -(2-cyclopentylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
-
IC50: 0.0537 mM, low inhibitory potential against EC 3.4.14.5
N1 -(4-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.66 nM
N1,N1-di(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 4.2 nM
N1,N1-dibenzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.6 nM
N1-(1-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.31 nM
N1-(2,4-dimethoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 2.7 nM
N1-(2-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.23 nM
N1-(2-cyclohexylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
-
IC50: 0.0546 mM, low inhibitory potential against EC 3.4.14.5
N1-(2-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.85 nM
N1-(2-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.1 nM
N1-(3-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.34 nM
N1-(3-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.77 nM
N1-(3-nitro-2-pyridinyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.0235 mM
N1-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.48 nM
N1-(4-chlorobenzyl)-N1-methyl-4-oxo-4-(1-piperidinyl)-1,3-butanediamine
-
IC50: 0.22 nm
N1-(cyclohexylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.1 nM
N1-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 2.03 nM
N1-cyclohexyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 18.6 nM
N1-[2-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.39 nM
N1-[4-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.41 nM
N2-cyclopentyl-N-[(diphenoxyphosphoryl)(phenyl)methyl]glycinamide
-
-
N3-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrobromide
-
IC50: 0.0125 mM
p-hydroxymercuribenzoate
-
-
p-Nitrophenyl-p-guanidinobenzoate
PCMBS
-
77% inhibition at 1 mM, preincubation at room temperature for 15 min
Pepstatin
-
0.01 mM, 11% inhibition
Phe-PSI[CS-N]-pyrrolidine
Phe-PSI[CS-N]-thiazolidine
Phe-pyrrolidine
Phe-thiazolidine
phenylmethylsulfonyl fluoride
Phenylmethylsulfonylfluoride
-
-
PMSF
-
45% inhibition at 1 mM, preincubation at room temperature for 15 min
puromycin
puromycin aminonucleoside
-
inhibition by cations increases with the size of the ion
Sodium deoxycholate
threo-Ile thiazolidide
-
not selective for the enzyme
Val pyrrolidide
-
not selective for the enzyme
Val thiazolidide
-
not selective for the enzyme
Val-PSI[CS-N]-pyrrolidine
Val-PSI[CS-N]-thiazolidine
Val-pyrrolidine
Val-thiazolidine
[(1R)-1-[[(2S)-2,4-diaminobutanoyl]amino]pentyl]boronic acid
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the Dpp2 gene is transcriptionally activated by Kruppel-like factor 2 and TOB1
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.487 - 1.22
Ala-Ala-4-nitroanilide
1.67
Ala-Ala-Ala
-
-
0.0457
Ala-Pro-4-nitroanilide
-
-
0.032
Ala-Pro-amino-4-trifluoromethylcoumarin
-
pH 7.5, 37C
0.009 - 0.041
Ala-Pro-p-nitroanilide
0.0086
Arg-Pro-2-naphthylamide
-
-
0.068 - 0.086
Arg-Pro-4-nitroanilide
0.04
Arg-Pro-p-nitroanilide
-
37C, pH 5.5
0.101
Asn-Pro-p-nitroanilide
-
37C, pH 5.5
1.16
Asp-Pro-4-nitroanilide
-
-
0.311
Glu-Pro-4-nitroanilide
-
-
5.55
Gly-Ala-Ala
-
-
0.36 - 1.1
Gly-L-Pro-4-nitroanilide
1.33
Gly-Pro-4-methylcoumarin 7-amide
-
-
0.018 - 0.59
Gly-Pro-4-nitroanilide
0.149
Gly-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37C
1.09
Gly-Pro-Ala
-
-
0.07 - 0.227
Gly-Pro-p-nitroanilide
0.0223
His-Ala-p-nitroanilide
-
37C, pH 5.5
0.083
His-Pro-p-nitroanilide
-
37C, pH 5.5
0.502
His-Pro-Val
-
enzyme form DPP II-S
0.087 - 0.555
Lys-Ala-2-naphthylamide
0.36 - 0.93
Lys-Ala-4-methylcoumarin 7-amide
0.215
Lys-Ala-7-amido-4-methylcoumarin
-
pH 7.5, 37C
0.04 - 0.57
Lys-Ala-p-nitroanilide
0.0457
Lys-Pro-4-nitroanilide
-
-
0.011
Lys-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37C
0.019
Lys-Pro-p-nitroanilide
0.02
Nle-Nle-p-nitroanilide
-
37C, pH 5.5
0.006
Nle-Nle-rhodamine
-
pH 7.5, 37C
0.003
Nle-Pro-7-amido-4-methylcoumarin
0.045
Phe-Pro-2-naphthylamide
-
-
0.058
Phe-Pro-p-nitroanilide
-
37C, pH 5.5
1.376
Pro-Ala-p-nitroanilide
-
37C, pH 5.5
0.08
Ser-Pro-p-nitroanilide
-
37C, pH 5.5
0.22
Tyr-Ala-p-nitroanilide
-
37C, pH 5.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
314
Ala-Ala-4-nitroanilide
Rattus norvegicus
-
-
121
Ala-Ala-Ala
Rattus norvegicus
-
-
89 - 105.4
Ala-Pro-p-nitroanilide
6.4
Arg-Pro-2-naphthylamide
Sus scrofa
-
-
65.8
Arg-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
184.6
Asn-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
103
Gly-Ala-Ala
Rattus norvegicus
-
-
6.8 - 11.5
Gly-L-Pro-4-nitroanilide
109
Gly-Pro-4-nitroanilide
Rattus norvegicus
-
-
120
Gly-Pro-Ala
Rattus norvegicus
-
-
67.3
Gly-Pro-p-nitroanilide
12.8
His-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
28.4
His-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
6 - 536
Lys-Ala-2-naphthylamide
229.3
Lys-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
3 - 77.7
Lys-Pro-p-nitroanilide
18.4
Nle-Nle-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
54
Phe-Pro-2-naphthylamide
Sus scrofa
-
-
93
Phe-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
15.3
Pro-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
66 - 116.9
Ser-Pro-p-nitroanilide
11
Tyr-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014 - 0.0033
Ala-PSI[CS-N]-pyrrolidine
0.00028 - 0.00065
Ala-PSI[CS-N]-thiazolidine
0.0218 - 0.023
Ala-pyrrolidine
0.0044 - 0.0052
Ala-thiazolidine
0.0408
Diprotin A
-
pH 7.5, 37C
0.00056
Ile cyanothiazolidide
-
pH 7.5, 37C
0.0048 - 0.0101
Ile-PSI[CS-N]-pyrrolidine
0.00091 - 0.0011
Ile-PSI[CS-N]-thiazolidine
0.0247 - 0.0302
Ile-pyrrolidine
0.0071 - 0.0082
Ile-thiazolidine
0.00065
Lys piperidide
-
pH 7.5, 37C
0.00017
Lys thiazolidide
-
pH 7.5, 37C
0.000052 - 0.00264
lysyl-piperidide
0.000082 - 0.00145
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
0.0073 - 0.0159
Phe-PSI[CS-N]-pyrrolidine
0.0006 - 0.0035
Phe-PSI[CS-N]-thiazolidine
0.0116 - 0.0139
Phe-pyrrolidine
0.0016 - 0.0032
Phe-thiazolidine
0.0097
threo-Ile thiazolidide
-
pH 7.5, 37C
0.1
Val pyrrolidide
-
above, pH 7.5, 37C
0.0153
Val thiazolidide
-
pH 7.5, 37C
0.005 - 0.0082
Val-PSI[CS-N]-pyrrolidine
0.00066 - 0.0009
Val-PSI[CS-N]-thiazolidine
0.0259 - 0.0267
Val-pyrrolidine
0.0057 - 0.0077
Val-thiazolidine
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0361
(1R,S)-(2E)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0361 mM, low inhibitory potential against EC 3.4.14.5
0.0404
(1R,S)-(2E)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0404 mM, low inhibitory potential against EC 3.4.14.5
0.0329
(1R,S)-(2Z)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0329 mM, low inhibitory potential against EC 3.4.14.5
0.0873
(1R,S)-(2Z)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0873 mM, low inhibitory potential against EC 3.4.14.5
0.00013
(3S)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
Homo sapiens
-
-
0.00000088
(3S)-4-[(3aR,6aR)-hexahydrocyclopenta[b]pyrrol-1(2H)-yl]-4-oxobutane-1,3-diamine
Homo sapiens
-
-
0.0000023 - 2.3
(3S)-N1-(4-chlorobenzyl)-4-(2-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
0.62
(3S)-N1-(4-chlorobenzyl)-4-(3-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 0.62 nM
0.00000113
(3S)-N1-(4-chlorobenzyl)-4-(3-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 1.13 nM
0.00000039
(3S)-N1-(4-chlorobenzyl)-4-(4-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 0.39 nM
0.00000055
(3S)-N1-(4-chlorobenzyl)-4-(4-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 0.55 nM
0.00000048 - 0.0000011
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
0.0000547
1-((2S)-2-amino-4-((4-chlorobenzyl)amino)butanoyl)piperidin-3-ol
Homo sapiens
-
IC50: 54.7 nM
0.000113
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidin-4-ol
Homo sapiens
-
IC50: 113 nM
0.0000065
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidine-2-carbonitrile
Homo sapiens
-
IC50: 6.5 nM
0.0255
1-([(4-fluorobenzyl)amino]acetyl)-2-pyrrolidinecarbonitrile
Homo sapiens
-
IC50: 0.0255 mM
0.00104
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
0.0013
1-benzyl-N-(2-cyclopentylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens
-
IC50: 0.0013 mM, low inhibitory potential against EC 3.4.14.5
0.0031
1-benzyl-N-(2-oxo-2-piperidin-1-ylethyl)piperidin-4-amine
0.1335
1-[(1-adamantylamino)acetyl]-2-pyrrolidinecarbonitrile
Homo sapiens
-
IC50: 0.1335 mM
0.000004
1-[(2S)-2-amino-4-(benzylamino)butanoyl]piperidine-2-carbonitrile
Homo sapiens
-
IC50: 4.0 nM
0.0000547
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-3-ol
Homo sapiens
-
IC50: 54.7 nM
0.000113
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-4-ol
Homo sapiens
-
IC50: 113 nM
0.0000065
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidine-2-carbonitrile
Homo sapiens
-
IC50: 6.5 nM
0.0000027
3-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)aminomethyl)-benzonitrile
Homo sapiens
-
IC50: 2.7 nM
0.000085
4-oxo-4-(1-piperidinyl)-N1 -(4-piperidinyl)-1,3-(S)-butanedi-amine
Homo sapiens
-
IC50: 85 nM
0.0000008
4-oxo-4-(1-piperidinyl)-N1-(2-thienylmethyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.8 nM
0.00000075
4-oxo-4-(1-piperidinyl)-N1-(4-pyridinylmethyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.75 nM
0.017
4-oxo-4-(1-piperidinyl)-N3-(4-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.017 mM
0.0000009
4-oxo-N1-(2-phenylethyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.9 nM
0.00000191
4-oxo-N1-(3-phenylpropyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.91 nM
0.0015
4-oxo-N1-phenyl-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.0015 mM
0.0046
6-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)amino)nicotinonitrile
Homo sapiens
-
IC50: 0.0046 mM
0.0128
benzyl 4-[(2-cyclohexylidene-2-fluoroethyl)amino]-1-piperidinecarboxylate
Homo sapiens
-
IC50: 0.0128 mM, low inhibitory potential against EC 3.4.14.5
0.0129
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclohexylidene-2-fluoroethanamine
Homo sapiens
-
IC50: 0.0129 mM, low inhibitory potential against EC 3.4.14.5
0.0075
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclopentylideneethanamine
Homo sapiens
-
IC50: 0.0075 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.0913
N-(2-cyclohexylidene-2-fluoroethyl)-1-adamantanamine
Homo sapiens
-
IC50: 0.0913 mM, low inhibitory potential against EC 3.4.14.5
0.0042
N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens
-
IC50: 0.0042 mM, low inhibitory potential against EC 3.4.14.5
0.011
N-(2-cyclohexylidene-2-fluoroethyl)-N-(2-phenylethyl)-amine
Homo sapiens
-
IC50: 0.011 mM, low inhibitory potential against EC 3.4.14.5
0.0219
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-fluorobenzyl)-amine
Homo sapiens
-
IC50: 0.0219 mM, low inhibitory potential against EC 3.4.14.5
0.0134
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-methoxybenzyl)amine
Homo sapiens
-
IC50: 0.0134 mM, low inhibitory potential against EC 3.4.14.5
0.1
N-(2-cyclohexylidene-2-fluoroethyl)aniline
Homo sapiens
-
IC50: 0.1 mM, low inhibitory potential against EC 3.4.14.5
0.5
N-(2-cyclohexylidene-2-fluoroethyl)cyclohexanamine
Homo sapiens
-
IC50: 0.5 mM, low inhibitory potential against EC 3.4.14.5
0.0272
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-phenylethyl)amine
Homo sapiens
-
IC50: 0.0272 mM, low inhibitory potential against EC 3.4.14.5
0.0096 - 0.0157
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-pyridinylethyl)amine
0.0108
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-fluorobenzyl)amine
Homo sapiens
-
IC50: 0.0108 mM, low inhibitory potential against EC 3.4.14.5
0.0155
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-methoxy-benzyl)amine
Homo sapiens
-
IC50: 0.0155 mM, low inhibitory potential against EC 3.4.14.5
0.09
N-(2-cyclopentylidene-2-fluoroethyl)cyclohexanamine
Homo sapiens
-
IC50: 0.09 mM, low inhibitory potential against EC 3.4.14.5
0.062
N-(2-cyclopentylideneethyl)cyclohexanamine
Homo sapiens
-
IC50: 0.062 mM; IC50: 0.062 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.034
N-benzyl-2-cyclohexylidene-2-fluoroethanamine
Homo sapiens
-
IC50: 0.034 mM, low inhibitory potential against EC 3.4.14.5
0.0378
N-benzyl-2-cyclopentylidene-2-fluoroethanamine
Homo sapiens
-
IC50: 0.0378 mM, low inhibitory potential against EC 3.4.14.5
0.026
N-benzyl-2-cyclopentylideneethanamine
Homo sapiens
-
IC50: 0.026 mM; IC50: 0.026 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.397
N-benzyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.397 mM, low inhibitory potential against EC 3.4.14.5
0.5
N-benzyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.5 mM
0.0484
N-benzyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.0484 mM, low inhibitory potential against EC 3.4.14.5
0.177
N-cyclohexyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.177 mM, low inhibitory potential against EC 3.4.14.5
0.276
N-cyclohexyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.276 mM
0.069
N-cyclohexyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.069 mM, low inhibitory potential against EC 3.4.14.5
0.092
N-cyclohexyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.092 mM, low inhibitory potential against EC 3.4.14.5
0.052
N-piperonyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.052 mM, low inhibitory potential against EC 3.4.14.5
0.063
N-piperonyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.063 mM, low inhibitory potential against EC 3.4.14.5
0.042
N-piperonyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.042 mM, low inhibitory potential against EC 3.4.14.5
0.067
N-piperonyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.067 mM, low inhibitory potential against EC 3.4.14.5
0.0015
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]acetamide
Homo sapiens
-
IC50: 0.0015 mM
0.0018
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]guanidine bis-(trifluoroacetate)
Homo sapiens
-
IC50: 0.0018 mM
0.0074
N-[3-amino-1-(S)-(1-piperidinylcarbonyl)propyl]guanidine bis-(trifluoroacetate)
0.0537
N1 -(2-cyclopentylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
Homo sapiens
-
IC50: 0.0537 mM, low inhibitory potential against EC 3.4.14.5
0.00000066
N1 -(4-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.66 nM
0.0000042
N1,N1-di(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 4.2 nM
0.0000006
N1,N1-dibenzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.6 nM
0.00000131
N1-(1-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.31 nM
0.0000027
N1-(2,4-dimethoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 2.7 nM
0.00000023
N1-(2-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.23 nM
0.0546
N1-(2-cyclohexylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
Homo sapiens
-
IC50: 0.0546 mM, low inhibitory potential against EC 3.4.14.5
0.00000185
N1-(2-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.85 nM
0.0000011
N1-(2-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.1 nM
0.00000134
N1-(3-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.34 nM
0.00000177
N1-(3-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.77 nM
0.0235
N1-(3-nitro-2-pyridinyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.0235 mM
0.00000048
N1-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.48 nM
0.00000022
N1-(4-chlorobenzyl)-N1-methyl-4-oxo-4-(1-piperidinyl)-1,3-butanediamine
Homo sapiens
-
IC50: 0.22 nm
0.0000011
N1-(cyclohexylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.1 nM
0.00000203
N1-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 2.03 nM
0.0000186
N1-cyclohexyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 18.6 nM
0.00000039
N1-[2-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.39 nM
0.00000041
N1-[4-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.41 nM
0.0038
N2-cyclopentyl-N-[(diphenoxyphosphoryl)(phenyl)methyl]glycinamide
Homo sapiens
-
-
0.0125
N3-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrobromide
Homo sapiens
-
IC50: 0.0125 mM
0.00000048
[(1R)-1-[[(2S)-2,4-diaminobutanoyl]amino]pentyl]boronic acid
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3
-
purified enzyme, substrate Ala-Ala-2-nitroanilide
0.803
-
-
1.05
-
purified enzyme, substrate Lys-Ala-7-amido-4-methylcoumarin
2.11
-
purified enzyme, substrate Gly-Pro-7-amido-4-methylcoumarin
2.85
-
purified enzyme, substrate Ala-Pro-amino-4-trifluoromethylcoumarin
28
-
-
70
-
substrate: Lys-Ala-p-nitroanilide
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 5.3
-
hydrolysis of tripeptides
5 - 5.7
-
hydrolysis of 2-naphthylamide derivatives
5 - 5.5
-
enzyme from seminal fluid
5
-
substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide
5.5 - 6
-
-
7 - 7.5
-
hydrolysis of Ala-2-naphthylamide
7.5 - 8.2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 11.5
-
-
3.5 - 8.5
-
-
3.7 - 5.8
-
pH 3.5: about 85% of maximal activity, pH 5.8: about 45% of maximal activity
4 - 6
-
pH 4.0: about 50% of maximal activity, pH 6.0: about 40% of maximal activity, substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide
4 - 7
-
50% of maximal activity at pH 4 and 7
5.5 - 7
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 4.8
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
mRNA expression in ipsilateral and contralateral cortices. Dipeptidyl peptidase II and aminopeptidase N are up-regulated ipsilaterally from 6 h to 7 days post ischemia
Manually annotated by BRENDA team
-
of renal convoluted tubules, of uterine glands
Manually annotated by BRENDA team
-
cytoplasm
Manually annotated by BRENDA team
-
of patients with Sjorgren's syndrome and healthy subjects
Manually annotated by BRENDA team
-
peritoneal, 10-20fold higher activity as compared with macrophages
Manually annotated by BRENDA team
-
smooth muscle cells
Manually annotated by BRENDA team
-
increased activity in serum of cancer patients
Manually annotated by BRENDA team
-
uterine myometrium
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
hepatocytes
Manually annotated by BRENDA team
-
enzyme form DPP II-S
Manually annotated by BRENDA team
-
enzyme form DPP II-M
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
105000
-
gel filtration and native PAGE
110000
116000
-
SDS-PAGE
120000
130000
135000
-
gel filtration
150000
-
enzyme from kidney cortex, in complex with adenosine deaminase, gel filtration
160000
-
gel filtration
185000
-
gel filtration
200000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
trimer
-
3 * 58000, SDS-PAGE in absence of 2-mercaptoethanol; 3 * 61000, SDS-PAGE in presence of 2-mercaptoethanol
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
side-chain modification
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
4C, 24 h, loss of activity of DPP II-M
95229
3.5 - 10
-
4C, 24 h, stable
95244
4 - 8
-
4C, 24 h, DPP II-M is stable
95229
4
-
25C, 24 h, 50% loss of activity
95233
5 - 7.5
-
stable
95246
5 - 7
-
25C, 72 h, stable
95233
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
2 h, 30% loss of activity without a stabilizer. 10% loss of activity after 4 h in presence of 1 mg/ml bovine serum albumin or 0.1% Tween 20
55
-
30 min, stable up to
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is stable during several repeated cycles of freezing and thawing
-
in presence of stabilizing agents, the enzyme tolerates at least five subsequent freeze/thaw cycles
-
resistant against several repeated cycles of freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, pH 6.0, 20 mM BisTris-HCl buffer, stable
-
-20C, stable for at least 3 months
-
-30C, 20 mM sodium phosphate-buffered saline, pH 8.0, stable for at least several months
-
-30C, stable for at least several months
-
4C, -20C, or -80C, purified enzyme is stable for at least 4 months in presence of 1 mg/ml bovine serum albumin
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
131.3fold to homogeneity, from kidney
-
ammonium sulfate precipitation and anion exchange chromatography
-
complexed with adenosine deaminase or dipeptidyl peptidase IV, DEAE cellulose column chromatography, DEAE-Sephadex A-50 gel filtration, Sephadex G-25 gel filtration, and Sephadex G-200 gel filtration
-
enzyme form DPP II-S and DPP II-M
-
from placenta
-
HisBind Resin column chromatography
-
partial, multiple isoforms
-
purification scheme includes an inhibitor-based affinity chromatographic step
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, sequence contains a leucine zipper motif
-
expressed in 293-EBNA cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
the enzyme is a potential urinary marker for the biochemical detection of kidney damage
medicine
-
mRNA expression in ipsilateral and contralateral cortices. Dipeptidyl peptidase II and aminopeptidase N are up-regulated ipsilaterally from 6 h to 7 days post ischemia. IPC1755, a non-selective protease inhibitor, reveals a significant reduction of cortical lesions after transient cerebral ischemia