Information on EC 3.4.14.2 - dipeptidyl-peptidase II

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.4.14.2
-
RECOMMENDED NAME
GeneOntology No.
dipeptidyl-peptidase II
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal dipeptide, Xaa-Yaa-/-, preferentially when Yaa is Ala or Pro. Substrates are oligopeptides, preferentially tripeptides
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
exopeptidase, N-terminus dipeptide
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
brain dipeptidyl aminopeptidase A
-
-
carboxytripeptidase
-
-
-
-
dipeptidyl aminopeptidase II
-
-
-
-
dipeptidyl aminopeptidase II
-
-
dipeptidyl aminopeptidase II
-
-
dipeptidyl aminopeptidase II
-
-
dipeptidyl arylamidase II
-
-
-
-
dipeptidyl arylamidase II
-
-
dipeptidyl peptidase
-
-
dipeptidyl peptidase
-
-
dipeptidyl peptidase 2
-
-
dipeptidyl peptidase II
-
-
-
-
dipeptidyl peptidase II
-
-
dipeptidyl peptidase II
-
-
dipeptidyl peptidase II
-
strongly prefers proline at P1 followed by norleucine
dipeptidyl peptidase II
-
-
dipeptidyl peptidase II
-
-
dipeptidyl peptidase II
-
-
dipeptidyl-aminopeptidase II
-
-
-
-
dipeptidylpeptidase II
-
-
-
-
DPP
-
-
DPP II
-
-
DPP-II
-
-
DPP2
-
-
DPP7
-
-
fibroblast activation protein alpha
-
has dipeptidyl peptidase and a 170-kDa gelatinase activity
peptidase, dipeptidyl, II
-
-
-
-
QPP
-
-
quiescent cell proline dipeptidase
-
-
-
-
quiescent cell proline dipeptidase
-
-
quiescent cell proline dipeptidase
-
-
quiescent cell proline dipeptidase
-
-
seprase
-
has dipeptidyl peptidase and a 170-kDa gelatinase activity
additional information
-
enzyme belongs to the Pro-X carboxypeptidase family S28
additional information
-
enzyme belongs to the Pro-X carboxypeptidase family S28
additional information
-
enzyme belongs to the Pro-X carboxypeptidase family S28
CAS REGISTRY NUMBER
COMMENTARY
76199-23-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
multiple isoforms
-
-
Manually annotated by BRENDA team
patients with Sjorgren's syndrome and healthy subjects
-
-
Manually annotated by BRENDA team
female and male Wistar rats
-
-
Manually annotated by BRENDA team
two enzyme forms: DPP II-M and DPP II-S
-
-
Manually annotated by BRENDA team
Streptomyces sp. WM-23
WM-23
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Ala)3 + H2O
?
show the reaction diagram
-
-
-
-
?
(Ala)3 + H2O
?
show the reaction diagram
-
-
-
-
?
(Ala)3 + H2O
?
show the reaction diagram
-
-
-
-
?
(Ala)3 + H2O
?
show the reaction diagram
-
L-Ala3
-
-
?
(Ala)4 + H2O
?
show the reaction diagram
-
-
-
-
?
(Gly-Pro-Ala)n + H2O
Gly-Pro + Ala
show the reaction diagram
-
-
-
?
Ala-Ala-2-naphthylamide + H2O
Ala-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Ala-Ala-2-naphthylamide + H2O
Ala-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Ala-Ala-2-naphthylamide + H2O
Ala-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Ala-Ala-2-nitroanilide + H2O
Ala-Ala + 2-nitroaniline
show the reaction diagram
-
low activity
-
?
Ala-Ala-4-nitroanilide + H2O
Ala-Ala + 4-nitroanilide
show the reaction diagram
-
-
-
?
Ala-Ala-4-nitroanilide + H2O
Ala-Ala + 4-nitroanilide
show the reaction diagram
-
-
-
?
Ala-Ala-Ala + H2O
Ala-Ala + Ala
show the reaction diagram
-
54% activity compared to Met-Ala-Ser
-
?
Ala-Ala-Ala-Ala + H2O
Ala-Ala + Ala-Ala
show the reaction diagram
-
39% activity compared to Met-Ala-Ser
-
?
Ala-Ala-p-nitroanilide + H2O
Ala-Ala + p-nitroaniline
show the reaction diagram
-
481% activity compared to Gly-Pro-7-amido-4-methylcoumarin
-
?
Ala-Ala-Phe-OH + H2O
Ala-Ala + Phe-OH
show the reaction diagram
Streptomyces sp., Streptomyces sp. WM-23
-
-
-
?
Ala-Phe-4-nitroanailide + H2O
Ala-Phe + 4-nitroaniline
show the reaction diagram
Streptomyces sp., Streptomyces sp. WM-23
-
-
-
?
Ala-Pro-2-(4-methoxy)-naphthylamine + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Pro-4-nitroanilide + H2O
Ala-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Ala-Pro-amino-4-trifluoromethylcoumarin + H2O
Ala-Pro + amino-4-trifluoromethylcoumarin
show the reaction diagram
-
-
?
Ala-Pro-amino-4-trifluoromethylcoumarin + H2O
Ala-Pro + amino-4-trifluoromethylcoumarin
show the reaction diagram
-
-
-
?
Ala-Pro-p-nitroanilide + H2O
Ala-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Ala-Pro-p-nitroanilide + H2O
Ala-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Arg-Ala-2-naphthylamide + H2O
Arg-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Arg-Ala-2-naphthylamide + H2O
Arg-Ala + 2-naphthylamine
show the reaction diagram
-
11% of the activity with Phe-Pro-2-naphthylamide
-
?
Arg-Pro-2-naphthylamide + H2O
Arg-Pro + 2-naphthylamine
show the reaction diagram
-
27% of the activity with Phe-Pro-2-naphthylamide
-
?
Arg-Pro-4-nitroanilide + H2O
Arg-Pro + 4-nitroanilide
show the reaction diagram
-
-
-
?
Arg-Pro-4-nitroanilide + H2O
Arg-Pro + 4-nitroanilide
show the reaction diagram
-
-
-
?
Arg-Pro-Lys-Pro + H2O
Arg-Pro + Lys-Pro
show the reaction diagram
-
fragments of substance P
-
?
Arg-Pro-p-nitroanilide + H2O
Arg-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Arg-Pro-p-nitroanilide + H2O
Arg-Pro + p-nitroaniline
show the reaction diagram
-
591% activity compared to Gly-Pro-7-amido-4-methylcoumarin
-
?
Asn-Pro-p-nitroanilide + H2O
Asn-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Asp-Pro-4-nitroanilide + H2O
Asp-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
bradykinin fragment 1-3 + H2O
?
show the reaction diagram
-
-
-
-
?
bradykinin fragment 1-5 + H2O
?
show the reaction diagram
-
-
-
-
?
casomorphin + H2O
?
show the reaction diagram
-
-
-
-
?
Glu-Pro-4-nitroanilide + H2O
Glu-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Glu-Pro-4-nitroanilide + H2O
Glu-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Ala-7-amido-4-methylcoumarin + H2O
Gly-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Gly-Ala-Ala + H2O
Gly-Ala + Ala
show the reaction diagram
-
-
-
?
Gly-L-Pro-4-nitroanilide + H2O
Gly-L-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Pro-2-(4-methoxy)-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Pro-2-naphthylamide + H2O
Gly-Pro + 2-naphthylamine
show the reaction diagram
-
-
-
?
Gly-Pro-4-methylcoumarin 7-amide + H2O
Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
-
specific for proline
-
?
Gly-Pro-7-amido-4-methylcoumarin + H2O
Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Gly-Pro-7-amido-4-methylcoumarin + H2O
Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Gly-Pro-7-amido-4-methylcoumarin + H2O
Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
100% activity
-
?
Gly-Pro-Ala + H2O
Gly-Pro + Ala
show the reaction diagram
-
-
-
?
Gly-Pro-Ala + H2O
Gly-Pro + Ala
show the reaction diagram
-
-
-
?
Gly-Pro-Ala + H2O
Gly-Pro + Ala
show the reaction diagram
-
96% activity compared to Met-Ala-Ser
-
?
Gly-Pro-Gly-Gly + H2O
Gly-Pro + Gly-Gly
show the reaction diagram
-
16% activity compared to Met-Ala-Ser
-
?
Gly-Pro-Leu-Gly + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Pro-p-nitroanilide + H2O
Gly-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Pro-p-nitroanilide + H2O
Gly-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Gly-Pro-p-nitroanilide + H2O
Gly-Pro + p-nitroaniline
show the reaction diagram
-
562% activity compared to Gly-Pro-7-amido-4-methylcoumarin
-
?
His-Ala-p-nitroanilide + H2O
His-Ala + p-nitroaniline
show the reaction diagram
-
-
-
?
His-Pro-p-nitroanilide + H2O
His-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
His-Pro-Val + H2O
His-Pro + Val
show the reaction diagram
-
-
-
?
L-Lys-L-Ala-5-chloro-1-anthraquinonylhydrazide-2HBr + H2O
L-Lys-L-Ala + 5-Cl-1-anthraquinonylhydrazine + HBr
show the reaction diagram
-
i.e. Lys-Ala-CAH, synthetic substrate for colorimetric assay
i.e. CAH, forms a highly insoluble, deeply colored hydrazone when coupled to an aromatic aldehyde, e.g. 4-nitrobenzaldehyde or piperonal, useful for histochemical detection of the enzyme
?
Leu-Ala-2-naphthylamide + H2O
Leu-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Leu-Ala-2-naphthylamide + H2O
Leu-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-(4-methoxy)-naphthylamide Lys-Ala-4-methoxy-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Ala-2-(4-methoxy)-naphthylamide Lys-Ala-4-methoxy-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Ala-2-naphthylamide + H2O
Lys-Ala + 2-naphthylamine
show the reaction diagram
-
15% of the activity with Phe-Pro-2-naphthylamide
-
?
Lys-Ala-4-methylcoumaryl-7-amide + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Lys-Ala-4-methylcoumaryl-7-amide + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Lys-Ala-4-methylcoumaryl-7-amide + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Lys-Ala-4-methylcoumaryl-7-amide + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Lys-Ala-7-amido-4-methylcoumarin + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Lys-Ala-7-amido-4-methylcoumarin + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Lys-Ala-7-amido-4-methylcoumarin + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
38% activity compared to Gly-Pro-7-amido-4-methylcoumarin
-
?
Lys-Ala-Ala + H2O
Lys-Ala + Ala
show the reaction diagram
-
-
-
?
Lys-Ala-Ala + H2O
Lys-Ala + Ala
show the reaction diagram
-
-
-
?
Lys-Ala-OCH3 + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Ala-p-nitroanilide + H2O
Lys-Ala + p-nitroaniline
show the reaction diagram
-
-
-
?
Lys-Ala-Pro + H2O
Lys-Ala + Pro
show the reaction diagram
-
-
-
?
Lys-Arg-2-naphthylamide + H2O
Lys-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Pro-2-(4-methoxy)-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Pro-2-(4-methoxy)-naphthylamine + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Pro-2-(4-methoxy)-naphthylamine + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Pro-2-naphthylamide + H2O
Lys-Pro + 2-naphthylamine
show the reaction diagram
-
-
-
?
Lys-Pro-2-naphthylamide + H2O
Lys-Pro + 2-naphthylamine
show the reaction diagram
-
46% of the activity with Phe-Pro-2-naphthylamide
-
?
Lys-Pro-4-nitroanilide + H2O
Lys-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Lys-Pro-4-nitroanilide + H2O
Lys-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
Lys-Pro-7-amido-4-methylcoumarin + H2O
Lys-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Lys-Pro-p-nitroanilide + H2O
Lys-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Lys-Pro-p-nitroanilide + H2O
Lys-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Met-Ala-Ser + H2O
Met-Ala + Ser
show the reaction diagram
-
100% activity
-
?
Met-Met-Ala + H2O
Met-Met + Ala
show the reaction diagram
-
-
-
?
Nle-Nle-p-nitroanilide + H2O
Nle-Nle + p-nitroaniline
show the reaction diagram
-
-
-
?
Nle-Nle-rhodamine + H2O
Nle-Nle + rhodamine
show the reaction diagram
-
-
?
Nle-Pro-7-amido-4-methylcoumarin + H2O
Nle-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Nle-Pro-rhodamine + H2O
Nle-Pro + rhodamine
show the reaction diagram
-
-
?
Phe-Pro-2-naphthylamide + H2O
Phe-Pro + 2-naphthylamine
show the reaction diagram
-
-
-
?
Phe-Pro-2-naphthylamide + H2O
Phe-Pro + 2-naphthylamine
show the reaction diagram
-
-
-
?
Phe-Pro-4-methylcoumarin 7-amide + H2O
Phe-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Phe-Pro-Ala + H2O
Phe-Pro + Ala
show the reaction diagram
-
-
-
?
Phe-Pro-p-nitroanilide + H2O
Phe-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Pro-Ala-p-nitroanilide + H2O
Pro-Ala + p-nitroaniline
show the reaction diagram
-
-
-
?
Ser-Met-Gln + H2O
Ser-Met + Gln
show the reaction diagram
-
-
-
?
Ser-Met-Glu + H2O
Ser-Met + Glu
show the reaction diagram
-
-
-
?
Ser-Met-OCH3 + H2O
?
show the reaction diagram
-
-
-
-
?
Ser-Pro-p-nitroanilide + H2O
Ser-Pro + p-nitroaniline
show the reaction diagram
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
-
-
-
?
Tyr-Ala-p-nitroanilide + H2O
Tyr-Ala + p-nitroaniline
show the reaction diagram
-
-
-
?
Val-Ala-4-nitroanilide + H2O
Val-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
?
Val-Ala-Phe-OH + H2O
Val-Ala + Phe-OH
show the reaction diagram
Streptomyces sp., Streptomyces sp. WM-23
-
-
-
?
Met-Met-Met + H2O
Met-Met + Met
show the reaction diagram
-
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
Pro and Arg are the preferred penultimate residues
-
-
-
additional information
?
-
-
no hydrolysis occurs if either the carboxyl group or the amino group of otherwise susceptible tripeptides is blocked. Tetrapeptides, tripeptide esters and dipeptides are not attacked
-
-
-
additional information
?
-
-
no activity with bradykinin
-
-
-
additional information
?
-
specific for Pro at position P1
-
-
?
additional information
?
-
-
the enzyme contributes to the alveolar macrophage's proteolytic capabilities
-
-
-
additional information
?
-
-
hydrolyzes specifically N-terminal X-alanine or X-proline from their respective arylamides and from tripeptides or tetrapeptides, but not from pentapeptides
-
-
-
additional information
?
-
-
possible role of the mast cell enzyme in remodelling of connective tissue
-
-
-
additional information
?
-
-
the enzyme may play a role in the segregation and degradation of specific regions of normal lens fiber cells
-
-
-
additional information
?
-
-
Ala and Pro are the preferred residues in the P1 position
-
-
-
additional information
?
-
-
no activity with Ala-Ala-Ala-Ala-Ala, Arg-Pro-Lys-Pro (substance P 1-4), and Arg-Pro-Pro-Gly-Phe (bradikinin1-5)
-
-
-
additional information
?
-
-
no activity with Gly-Arg-7-amido-4-methylcoumarin, Arg-Arg-7-amido-4-methylcoumarin, and Ala-Arg-7-amido-4-methylcoumarin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Arg-Pro-Lys-Pro + H2O
Arg-Pro + Lys-Pro
show the reaction diagram
-
fragments of substance P
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
-
-
-
?
casomorphin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme contributes to the alveolar macrophage's proteolytic capabilities
-
-
-
additional information
?
-
-
hydrolyzes specifically N-terminal X-alanine or X-proline from their respective arylamides and from tripeptides or tetrapeptides, but not from pentapeptides
-
-
-
additional information
?
-
-
possible role of the mast cell enzyme in remodelling of connective tissue
-
-
-
additional information
?
-
-
the enzyme may play a role in the segregation and degradation of specific regions of normal lens fiber cells
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(1R,S)-(2E)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0361 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2E)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0404 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2Z)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0329 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2Z)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0873 mM, low inhibitory potential against EC 3.4.14.5
(3S)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
-
-
(3S)-4-[(3aR,6aR)-hexahydrocyclopenta[b]pyrrol-1(2H)-yl]-4-oxobutane-1,3-diamine
-
AX8819, potent and selective inhibitor of DPP II
(3S)-N1-(4-chlorobenzyl)-4-(2-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 2.3 mM; IC50: 2.3 nM
(3S)-N1-(4-chlorobenzyl)-4-(3-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
-
0.62 nM; IC50: 0.62 nM
(3S)-N1-(4-chlorobenzyl)-4-(3-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 1.13 nM
(3S)-N1-(4-chlorobenzyl)-4-(4-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 0.39 nM
(3S)-N1-(4-chlorobenzyl)-4-(4-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 0.55 nM
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
-
IC50: 1.1 nM
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
-
UAMC00039, potent and specific inhibitor
(D-Ala)3
-
5 mM, 30% inhibition of hydrolysis of (L-Ala)3, noncompetitive
1-((2S)-2-amino-4-((4-chlorobenzyl)amino)butanoyl)piperidin-3-ol
-
IC50: 54.7 nM
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidin-4-ol
-
IC50: 113 nM
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidine-2-carbonitrile
-
IC50: 6.5 nM
1-([(2-phenylethyl)amino]acetyl)-2-pyrrolidinecarbonitrile
-
0.0163 mM
1-([(4-fluorobenzyl)amino]acetyl)-2-pyrrolidinecarbonitrile
-
IC50: 0.0255 mM
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.00104 mM, low inhibitory potential against EC 3.4.14.5
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)piperidin-4-amine
-
-
1-benzyl-N-(2-cyclopentylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.0013 mM, low inhibitory potential against EC 3.4.14.5
1-benzyl-N-(2-oxo-2-piperidin-1-ylethyl)piperidin-4-amine
-
-
1-benzyl-N-[2-oxo-2-(1-piperidinyl)ethyl]-4-piperidinamine
-
IC50: 0.0031 mM, low inhibitory potential against EC 3.4.14.5
1-[(1-adamantylamino)acetyl]-2-pyrrolidinecarbonitrile
-
IC50: 0.1335 mM
1-[(2S)-2-amino-4-(benzylamino)butanoyl]piperidine-2-carbonitrile
-
IC50: 4.0 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-3-ol
-
IC50: 54.7 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-4-ol
-
IC50: 113 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidine-2-carbonitrile
-
IC50: 6.5 nM
3-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)aminomethyl)-benzonitrile
-
IC50: 2.7 nM
4-(2-aminoethyl)benzenesulfonyl fluoride
-
60% inhibition at 1 mM, preincubation at room temperature for 15 min
4-(2-aminoethyl)benzenesulfonyl fluoride
-
-
4-Chloromercuriphenylsulfonate
-
-
4-Chloromercuriphenylsulfonate
-
-
4-oxo-4-(1-piperidinyl)-N1 -(4-piperidinyl)-1,3-(S)-butanedi-amine
-
IC50: 85 nM
4-oxo-4-(1-piperidinyl)-N1-(2-thienylmethyl)-1,3-(S)-butanediamine
-
IC50: 0.8 nM
4-oxo-4-(1-piperidinyl)-N1-(4-pyridinylmethyl)-1,3-(S)-butanediamine
-
IC50: 0.75 nM
4-oxo-4-(1-piperidinyl)-N3-(4-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.017 mM
4-oxo-N1-(2-phenylethyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.9 nM
4-oxo-N1-(3-phenylpropyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.91 nM
4-oxo-N1-phenyl-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.0015 mM
6-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)amino)nicotinonitrile
-
IC50: 0.0046 mM
Ala-PSI[CS-N]-pyrrolidine
-
competitive
Ala-PSI[CS-N]-thiazolidine
-
competitive
Ala-pyrrolidine
-
competitive
Ala-thiazolidine
-
competitive
ammonium sulfate
-
-
benzamidine
-
10% inhibition at 1 mM, preincubation at room temperature for 15 min
benzethonium chloride
-
-
benzyl 4-[(2-cyclohexylidene-2-fluoroethyl)amino]-1-piperidinecarboxylate
-
IC50: 0.0128 mM, low inhibitory potential against EC 3.4.14.5
bestatin
-
1 mM, 12% inhibition of DPP II-S, 21% inhibition of DPP II-M; no inhibition
bestatin
-
-
Cd2+
-
-
Cd2+
-
moderate inhibition
Cetyltrimethylammonium bromide
-
-
Co2+
-
-
Co2+
-
moderate inhibition
Cu2+
-
-
Cu2+
-
40% inhibition at 1 mM, preincubation at room temperature for 15 min
Cu2+
-
significant inhibition
Cu2+
-
10 mM CuSO4, 23% inhibition
Cu2+
-
-
diaminobutyric acid-piperidide
-
-
diisopropyl fluorophosphate
-
-
diisopropyl fluorophosphate
-
-
diisopropyl fluorophosphate
-
-
diisopropyl fluorophosphate
-
-
diisopropylfluorophosphate
-
complete inhibition at 1 mM, 25% inhibition at 0.1 mM, preincubation at room temperature for 15 min
Diprotin A
-
not selective for the enzyme
EDTA
-
-
epostatin
-
-
epsilon-benzoyloxycarbonyl-Lys-Pro
-
1 mM, 31% inhibition of DPP II-S, 41% inhibition of DPP II-M
-
epsilon-Z-Lys-Pro
-
competitive inhibitor
Fe2+
-
significant inhibition
Hg2+
-
-
Hg2+
-
-
Hg2+
-
complete inhibition at 1 mM, preincubation at room temperature for 15 min
Hg2+
-
significant inhibition
Hg2+
-
HgCl2
Hg2+
-
HgCl2
histamine
-
-
histamine
-
0.1 M 55% inhibition
hyamine 10-X
-
-
Ile cyanothiazolidide
-
not selective for the enzyme
Ile-PSI[CS-N]-pyrrolidine
-
competitive
Ile-PSI[CS-N]-thiazolidine
-
competitive
Ile-pyrrolidine
-
competitive
Ile-thiazolidine
-
competitive
iodoacetate
-
weak
K+
-
50 mM KCl, 42% inhibition
K+
-
inhibition by cations increases with the size of the ion
Li+
-
-
Li+
-
inhibition by cations increases with the size of the ion
Lys piperidide
-
not selective for the enzyme
Lys thiazolidide
-
not selective for the enzyme
Lys-Ala chloromethyl ketone
-
0.1 mM, complete inhibition, active-site-directed inhibitor
Lys-Ala-CH2Cl
-
inhibitory at 0.1 mM
lysyl-piperidide
-
-
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclohexylidene-2-fluoroethanamine
-
IC50: 0.0129 mM, low inhibitory potential against EC 3.4.14.5
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclopentylideneethanamine
-
0.0075 mM; IC50: 0.0075 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-1-adamantanamine
-
IC50: 0.0913 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.0042 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(2-phenylethyl)-amine
-
IC50: 0.011 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-fluorobenzyl)-amine
-
IC50: 0.0219 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-methoxybenzyl)amine
-
IC50: 0.0134 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)aniline
-
IC50: 0.1 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)cyclohexanamine
-
IC50: 0.5 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-phenylethyl)amine
-
IC50: 0.0272 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-pyridinylethyl)amine
-
IC50: 0.0096 mM, low inhibitory potential against EC 3.4.14.5; IC50: 0.0157 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-fluorobenzyl)amine
-
IC50: 0.0108 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-methoxy-benzyl)amine
-
IC50: 0.0155 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)cyclohexanamine
-
IC50: 0.09 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylideneethyl)cyclohexanamine
-
IC50: 0.062 mM; IC50: 0.062 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
-
N-4(chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrochloride
-
-
N-benzyl-2-cyclohexylidene-2-fluoroethanamine
-
IC50: 0.034 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-cyclopentylidene-2-fluoroethanamine
-
IC50: 0.0378 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-cyclopentylideneethanamine
-
IC50: 0.026 mM; IC50: 0.026 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.397 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.5 mM
N-benzyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.0484 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.177 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.276 mM
N-cyclohexyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.069 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.092 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.052 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.063 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.042 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.067 mM, low inhibitory potential against EC 3.4.14.5
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]acetamide
-
IC50: 0.0015 mM
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]guanidine bis-(trifluoroacetate)
-
IC50: 0.0018 mM
N-[3-amino-1-(S)-(1-piperidinylcarbonyl)propyl]guanidine bis-(trifluoroacetate)
-
IC50: 0.0074 mM
N-[3-amino-4-oxo-4-(1-piperidinyl)butyl]benzamide
-
IC50: 0.0074 mM
N1 -(2-cyclopentylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
-
IC50: 0.0537 mM, low inhibitory potential against EC 3.4.14.5
N1 -(4-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.66 nM
N1,N1-di(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 4.2 nM
N1,N1-dibenzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.6 nM
N1-(1-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.31 nM
N1-(2,4-dimethoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 2.7 nM
N1-(2-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.23 nM
N1-(2-cyclohexylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
-
IC50: 0.0546 mM, low inhibitory potential against EC 3.4.14.5
N1-(2-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.85 nM
N1-(2-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.1 nM
N1-(3-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.34 nM
N1-(3-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.77 nM
N1-(3-nitro-2-pyridinyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.0235 mM
N1-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.48 nM
N1-(4-chlorobenzyl)-N1-methyl-4-oxo-4-(1-piperidinyl)-1,3-butanediamine
-
IC50: 0.22 nm
N1-(cyclohexylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.1 nM
N1-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 2.03 nM
N1-cyclohexyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 18.6 nM
N1-[2-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.39 nM
N1-[4-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.41 nM
N2-cyclopentyl-N-[(diphenoxyphosphoryl)(phenyl)methyl]glycinamide
-
-
N3-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrobromide
-
IC50: 0.0125 mM
Na+
-
400 mM NaCl, 56% inhibition of DPP II-M, 31% inhibition of DPP II-S
Na+
-
10 mM NaCl, 38% inhibition
Na+
-
100 mM NaCl, 11% inhibition
Na+
-
inhibition by cations increases with the size of the ion
p-hydroxymercuribenzoate
-
-
p-Nitrophenyl-p-guanidinobenzoate
-
1 mM, 43% inhibition
p-Nitrophenyl-p-guanidinobenzoate
-
-
p-Nitrophenyl-p-guanidinobenzoate
-
-
Pb2+
-
moderate inhibition
PCMBS
-
77% inhibition at 1 mM, preincubation at room temperature for 15 min
Pepstatin
-
0.01 mM, 11% inhibition
Phe-PSI[CS-N]-pyrrolidine
-
competitive
Phe-PSI[CS-N]-thiazolidine
-
competitive
Phe-pyrrolidine
-
competitive
Phe-thiazolidine
-
competitive
phenylmethylsulfonyl fluoride
-
5 mM, about 80% inhibition
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
1 mM, 15% inhibition
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
Phenylmethylsulfonylfluoride
-
-
PMSF
-
45% inhibition at 1 mM, preincubation at room temperature for 15 min
puromycin
-
1 mM, 47% inhibition
puromycin
-
1 mM, 76% inhibition; inhibition by cations increases with the size of the ion
puromycin
-
-
puromycin aminonucleoside
-
inhibition by cations increases with the size of the ion
Sodium deoxycholate
-
-
Sodium deoxycholate
-
-
threo-Ile thiazolidide
-
not selective for the enzyme
Tris
-
50 mM, 97% inhibition
Tris
-
50 mM, 55% inhibition
Tris
-
50 mM, 96% inhibition; inhibition by cations increases with the size of the ion
Val pyrrolidide
-
not selective for the enzyme
Val thiazolidide
-
not selective for the enzyme
Val-PSI[CS-N]-pyrrolidine
-
competitive
Val-PSI[CS-N]-thiazolidine
-
competitive
Val-pyrrolidine
-
competitive
Val-thiazolidine
-
competitive
Zn2+
-
moderate inhibition
Zn2+
-
10 mM, 83% inhibition
[(1R)-1-[[(2S)-2,4-diaminobutanoyl]amino]pentyl]boronic acid
-
-
lysyl-piperidide
-
-
additional information
-
isomerization of the thiazolidine derivatives
-
additional information
-
poor or no inhibition by EDTA, NEM, E-64, diprotin A, and actinonin
-
additional information
-
not inhibited by N-ethylmaleimide, p-chloromercuribenzoate, iodoacetate, iodoacetamide, beta-mercaptoethanol, cysteine, EDTA, EGTA, o-phenanthroline, or dithiothreitol
-
additional information
-
not inhibited by diprotin A
-
additional information
-
no inhibition by bacitracin
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
the Dpp2 gene is transcriptionally activated by Kruppel-like factor 2 and TOB1
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.487
Ala-Ala-4-nitroanilide
-
-
1.22
Ala-Ala-4-nitroanilide
-
-
1.67
Ala-Ala-Ala
-
-
0.0457
Ala-Pro-4-nitroanilide
-
-
0.032
Ala-Pro-amino-4-trifluoromethylcoumarin
-
pH 7.5, 37C
0.009
Ala-Pro-p-nitroanilide
-
37C, pH 7.0
0.04
Ala-Pro-p-nitroanilide
-
37C, pH 5.5
0.041
Ala-Pro-p-nitroanilide
-
-
0.0086
Arg-Pro-2-naphthylamide
-
-
0.068
Arg-Pro-4-nitroanilide
-
enzyme form DPP II-M
0.086
Arg-Pro-4-nitroanilide
-
-
0.04
Arg-Pro-p-nitroanilide
-
37C, pH 5.5
0.101
Asn-Pro-p-nitroanilide
-
37C, pH 5.5
1.16
Asp-Pro-4-nitroanilide
-
-
0.311
Glu-Pro-4-nitroanilide
-
-
5.55
Gly-Ala-Ala
-
-
0.36
Gly-L-Pro-4-nitroanilide
-
enzyme from renal cortex, in acetic buffer (pH 5.5), at 37C
1.1
Gly-L-Pro-4-nitroanilide
-
enzyme from lung, in acetic buffer (pH 5.5), at 37C
1.33
Gly-Pro-4-methylcoumarin 7-amide
-
-
0.018
Gly-Pro-4-nitroanilide
-
enzyme form DPP II-S
0.032
Gly-Pro-4-nitroanilide
-
enzyme form DPP II-M
0.25
Gly-Pro-4-nitroanilide
-
pH 5.5, 30C
0.282
Gly-Pro-4-nitroanilide
-
-
0.59
Gly-Pro-4-nitroanilide
-
pH 5.5, 30C
0.149
Gly-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37C
1.09
Gly-Pro-Ala
-
-
0.07
Gly-Pro-p-nitroanilide
-
37C, pH 7-0
0.21
Gly-Pro-p-nitroanilide
-
37C, pH 5.5
0.227
Gly-Pro-p-nitroanilide
-
-
0.0223
His-Ala-p-nitroanilide
-
37C, pH 5.5
0.083
His-Pro-p-nitroanilide
-
37C, pH 5.5
0.502
His-Pro-Val
-
enzyme form DPP II-S
0.087
Lys-Ala-2-naphthylamide
-
enzyme form DPP II-S
0.099
Lys-Ala-2-naphthylamide
-
-
0.21
Lys-Ala-2-naphthylamide
-
enzyme form DPP II-M
0.555
Lys-Ala-2-naphthylamide
-
-
0.36
Lys-Ala-4-methylcoumarin 7-amide
-
-
0.93
Lys-Ala-4-methylcoumarin 7-amide
-
-
0.215
Lys-Ala-7-amido-4-methylcoumarin
-
pH 7.5, 37C
0.04
Lys-Ala-p-nitroanilide
-
37C, pH 7.0
0.57
Lys-Ala-p-nitroanilide
-
37C, pH 5.5
0.0457
Lys-Pro-4-nitroanilide
-
-
0.011
Lys-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37C
0.019
Lys-Pro-p-nitroanilide
-
37C, pH 5.5
0.019
Lys-Pro-p-nitroanilide
-
-
0.02
Nle-Nle-p-nitroanilide
-
37C, pH 5.5
0.006
Nle-Nle-rhodamine
-
pH 7.5, 37C
0.003
Nle-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37C
0.003
Nle-Pro-rhodamine
-
pH 7.5, 37C
0.045
Phe-Pro-2-naphthylamide
-
-
0.058
Phe-Pro-p-nitroanilide
-
37C, pH 5.5
1.376
Pro-Ala-p-nitroanilide
-
37C, pH 5.5
0.08
Ser-Pro-p-nitroanilide
-
37C, pH 5.5
0.22
Tyr-Ala-p-nitroanilide
-
37C, pH 5.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
314
Ala-Ala-4-nitroanilide
Rattus norvegicus
-
-
121
Ala-Ala-Ala
Rattus norvegicus
-
-
89
Ala-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
89
Ala-Pro-p-nitroanilide
Homo sapiens
-
-
105.4
Ala-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
105.4
Ala-Pro-p-nitroanilide
Homo sapiens
-
-
6.4
Arg-Pro-2-naphthylamide
Sus scrofa
-
-
65.8
Arg-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
184.6
Asn-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
103
Gly-Ala-Ala
Rattus norvegicus
-
-
6.8
Gly-L-Pro-4-nitroanilide
Bos taurus
-
enzyme from lung, in acetic buffer (pH 5.5), at 37C
11.5
Gly-L-Pro-4-nitroanilide
Bos taurus
-
enzyme from renal cortex, in acetic buffer (pH 5.5), at 37C
109
Gly-Pro-4-nitroanilide
Rattus norvegicus
-
-
120
Gly-Pro-Ala
Rattus norvegicus
-
-
67.3
Gly-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
67.3
Gly-Pro-p-nitroanilide
Homo sapiens
-
-
12.8
His-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
28.4
His-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
6
Lys-Ala-2-naphthylamide
Sus scrofa
-
-
536
Lys-Ala-2-naphthylamide
Rattus norvegicus
-
-
229.3
Lys-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
3
Lys-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
3
Lys-Pro-p-nitroanilide
Homo sapiens
-
-
77.7
Lys-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
77.7
Lys-Pro-p-nitroanilide
Homo sapiens
-
-
18.4
Nle-Nle-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
54
Phe-Pro-2-naphthylamide
Sus scrofa
-
-
93
Phe-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
15.3
Pro-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
66
Ser-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
116.9
Ser-Pro-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
11
Tyr-Ala-p-nitroanilide
Homo sapiens
-
37C, pH 5.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0014
Ala-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.0033
Ala-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.00028
Ala-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.00065
Ala-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.0218
Ala-pyrrolidine
-
pH 5.5, 30C
0.023
Ala-pyrrolidine
-
pH 5.5, 30C
0.0044
Ala-thiazolidine
-
pH 5.5, 30C
0.0052
Ala-thiazolidine
-
pH 5.5, 30C
0.0408
Diprotin A
-
pH 7.5, 37C
0.00056
Ile cyanothiazolidide
-
pH 7.5, 37C
0.0048
Ile-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.0101
Ile-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.00091
Ile-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.0011
Ile-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.0247
Ile-pyrrolidine
-
pH 5.5, 30C
0.0302
Ile-pyrrolidine
-
pH 5.5, 30C
0.0071
Ile-thiazolidine
-
pH 5.5, 30C
0.0082
Ile-thiazolidine
-
pH 5.5, 30C
0.00065
Lys piperidide
-
pH 7.5, 37C
0.00017
Lys thiazolidide
-
pH 7.5, 37C
0.000052
lysyl-piperidide
-
substrate: Lys-Ala-p-nitroanilide, pH 7.0, 37C
0.000063
lysyl-piperidide
-
substrate: Ala-Pro-p-nitroanilide, pH 7.0, 37C
0.00172
lysyl-piperidide
-
substrate: Lys-Ala-p-nitroanilide, pH 5.5, 37C
0.00264
lysyl-piperidide
-
substrate: Ala-Pro-p-nitroanilide, pH 5., 37C
0.000082
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
-
0.00095
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
substrate: Lys-Ala-p-nitroanilide, pH 5.5, 37C
0.001
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
substrate: Lys-Ala-p-nitroanilide, pH 7.0, 37C
0.00105
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
substrate: Ala-Pro-p-nitroanilide, pH 7.0, 37C
0.00145
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
substrate: Ala-Pro-p-nitroanilide, pH 5.5, 37C
0.0073
Phe-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.0159
Phe-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.0006
Phe-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.0035
Phe-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.0116
Phe-pyrrolidine
-
pH 5.5, 30C
0.0139
Phe-pyrrolidine
-
pH 5.5, 30C
0.0016
Phe-thiazolidine
-
pH 5.5, 30C
0.0032
Phe-thiazolidine
-
pH 5.5, 30C
0.0097
threo-Ile thiazolidide
-
pH 7.5, 37C
0.1
Val pyrrolidide
-
above, pH 7.5, 37C
0.0153
Val thiazolidide
-
pH 7.5, 37C
0.005
Val-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.0082
Val-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30C
0.00066
Val-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.0009
Val-PSI[CS-N]-thiazolidine
-
pH 5.5, 30C
0.0259
Val-pyrrolidine
-
pH 5.5, 30C
0.0267
Val-pyrrolidine
-
pH 5.5, 30C
0.0057
Val-thiazolidine
-
pH 5.5, 30C
0.0077
Val-thiazolidine
-
pH 5.5, 30C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0361
(1R,S)-(2E)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0361 mM, low inhibitory potential against EC 3.4.14.5
0.0404
(1R,S)-(2E)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0404 mM, low inhibitory potential against EC 3.4.14.5
0.0329
(1R,S)-(2Z)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0329 mM, low inhibitory potential against EC 3.4.14.5
0.0873
(1R,S)-(2Z)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens
-
IC50: 0.0873 mM, low inhibitory potential against EC 3.4.14.5
0.00013
(3S)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
Homo sapiens
-
-
0.00000088
(3S)-4-[(3aR,6aR)-hexahydrocyclopenta[b]pyrrol-1(2H)-yl]-4-oxobutane-1,3-diamine
Homo sapiens
-
-
0.0000023
(3S)-N1-(4-chlorobenzyl)-4-(2-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 2.3 nM
2.3
(3S)-N1-(4-chlorobenzyl)-4-(2-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 2.3 mM
0.62
(3S)-N1-(4-chlorobenzyl)-4-(3-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 0.62 nM
0.00000113
(3S)-N1-(4-chlorobenzyl)-4-(3-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 1.13 nM
0.00000039
(3S)-N1-(4-chlorobenzyl)-4-(4-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 0.39 nM
0.00000055
(3S)-N1-(4-chlorobenzyl)-4-(4-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens
-
IC50: 0.55 nM
0.00000048
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
Homo sapiens
-
-
0.0000011
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
Homo sapiens
-
IC50: 1.1 nM
0.0000547
1-((2S)-2-amino-4-((4-chlorobenzyl)amino)butanoyl)piperidin-3-ol
Homo sapiens
-
IC50: 54.7 nM
0.000113
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidin-4-ol
Homo sapiens
-
IC50: 113 nM
0.0000065
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidine-2-carbonitrile
Homo sapiens
-
IC50: 6.5 nM
0.0255
1-([(4-fluorobenzyl)amino]acetyl)-2-pyrrolidinecarbonitrile
Homo sapiens
-
IC50: 0.0255 mM
0.00104
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens
-
IC50: 0.00104 mM, low inhibitory potential against EC 3.4.14.5
0.00104
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)piperidin-4-amine
Homo sapiens
-
-
0.0013
1-benzyl-N-(2-cyclopentylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens
-
IC50: 0.0013 mM, low inhibitory potential against EC 3.4.14.5
0.0031
1-benzyl-N-(2-oxo-2-piperidin-1-ylethyl)piperidin-4-amine
Homo sapiens
-
-
0.0031
1-benzyl-N-[2-oxo-2-(1-piperidinyl)ethyl]-4-piperidinamine
Homo sapiens
-
IC50: 0.0031 mM, low inhibitory potential against EC 3.4.14.5
0.1335
1-[(1-adamantylamino)acetyl]-2-pyrrolidinecarbonitrile
Homo sapiens
-
IC50: 0.1335 mM
0.000004
1-[(2S)-2-amino-4-(benzylamino)butanoyl]piperidine-2-carbonitrile
Homo sapiens
-
IC50: 4.0 nM
0.0000547
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-3-ol
Homo sapiens
-
IC50: 54.7 nM
0.000113
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-4-ol
Homo sapiens
-
IC50: 113 nM
0.0000065
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidine-2-carbonitrile
Homo sapiens
-
IC50: 6.5 nM
0.0000027
3-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)aminomethyl)-benzonitrile
Homo sapiens
-
IC50: 2.7 nM
0.000085
4-oxo-4-(1-piperidinyl)-N1 -(4-piperidinyl)-1,3-(S)-butanedi-amine
Homo sapiens
-
IC50: 85 nM
0.0000008
4-oxo-4-(1-piperidinyl)-N1-(2-thienylmethyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.8 nM
0.00000075
4-oxo-4-(1-piperidinyl)-N1-(4-pyridinylmethyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.75 nM
0.017
4-oxo-4-(1-piperidinyl)-N3-(4-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.017 mM
0.0000009
4-oxo-N1-(2-phenylethyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.9 nM
0.00000191
4-oxo-N1-(3-phenylpropyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.91 nM
0.0015
4-oxo-N1-phenyl-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.0015 mM
0.0046
6-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)amino)nicotinonitrile
Homo sapiens
-
IC50: 0.0046 mM
0.0128
benzyl 4-[(2-cyclohexylidene-2-fluoroethyl)amino]-1-piperidinecarboxylate
Homo sapiens
-
IC50: 0.0128 mM, low inhibitory potential against EC 3.4.14.5
0.0129
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclohexylidene-2-fluoroethanamine
Homo sapiens
-
IC50: 0.0129 mM, low inhibitory potential against EC 3.4.14.5
0.0075
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclopentylideneethanamine
Homo sapiens
-
IC50: 0.0075 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.0913
N-(2-cyclohexylidene-2-fluoroethyl)-1-adamantanamine
Homo sapiens
-
IC50: 0.0913 mM, low inhibitory potential against EC 3.4.14.5
0.0042
N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens
-
IC50: 0.0042 mM, low inhibitory potential against EC 3.4.14.5
0.011
N-(2-cyclohexylidene-2-fluoroethyl)-N-(2-phenylethyl)-amine
Homo sapiens
-
IC50: 0.011 mM, low inhibitory potential against EC 3.4.14.5
0.0219
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-fluorobenzyl)-amine
Homo sapiens
-
IC50: 0.0219 mM, low inhibitory potential against EC 3.4.14.5
0.0134
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-methoxybenzyl)amine
Homo sapiens
-
IC50: 0.0134 mM, low inhibitory potential against EC 3.4.14.5
0.1
N-(2-cyclohexylidene-2-fluoroethyl)aniline
Homo sapiens
-
IC50: 0.1 mM, low inhibitory potential against EC 3.4.14.5
0.5
N-(2-cyclohexylidene-2-fluoroethyl)cyclohexanamine
Homo sapiens
-
IC50: 0.5 mM, low inhibitory potential against EC 3.4.14.5
0.0272
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-phenylethyl)amine
Homo sapiens
-
IC50: 0.0272 mM, low inhibitory potential against EC 3.4.14.5
0.0096
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-pyridinylethyl)amine
Homo sapiens
-
IC50: 0.0096 mM, low inhibitory potential against EC 3.4.14.5
0.0157
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-pyridinylethyl)amine
Homo sapiens
-
IC50: 0.0157 mM, low inhibitory potential against EC 3.4.14.5
0.0108
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-fluorobenzyl)amine
Homo sapiens
-
IC50: 0.0108 mM, low inhibitory potential against EC 3.4.14.5
0.0155
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-methoxy-benzyl)amine
Homo sapiens
-
IC50: 0.0155 mM, low inhibitory potential against EC 3.4.14.5
0.09
N-(2-cyclopentylidene-2-fluoroethyl)cyclohexanamine
Homo sapiens
-
IC50: 0.09 mM, low inhibitory potential against EC 3.4.14.5
0.062
N-(2-cyclopentylideneethyl)cyclohexanamine
Homo sapiens
-
IC50: 0.062 mM; IC50: 0.062 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.034
N-benzyl-2-cyclohexylidene-2-fluoroethanamine
Homo sapiens
-
IC50: 0.034 mM, low inhibitory potential against EC 3.4.14.5
0.0378
N-benzyl-2-cyclopentylidene-2-fluoroethanamine
Homo sapiens
-
IC50: 0.0378 mM, low inhibitory potential against EC 3.4.14.5
0.026
N-benzyl-2-cyclopentylideneethanamine
Homo sapiens
-
IC50: 0.026 mM; IC50: 0.026 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.397
N-benzyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.397 mM, low inhibitory potential against EC 3.4.14.5
0.5
N-benzyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.5 mM
0.0484
N-benzyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.0484 mM, low inhibitory potential against EC 3.4.14.5
0.177
N-cyclohexyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.177 mM, low inhibitory potential against EC 3.4.14.5
0.276
N-cyclohexyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.276 mM
0.069
N-cyclohexyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.069 mM, low inhibitory potential against EC 3.4.14.5
0.092
N-cyclohexyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.092 mM, low inhibitory potential against EC 3.4.14.5
0.052
N-piperonyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.052 mM, low inhibitory potential against EC 3.4.14.5
0.063
N-piperonyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.063 mM, low inhibitory potential against EC 3.4.14.5
0.042
N-piperonyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens
-
IC50: 0.042 mM, low inhibitory potential against EC 3.4.14.5
0.067
N-piperonyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens
-
IC50: 0.067 mM, low inhibitory potential against EC 3.4.14.5
0.0015
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]acetamide
Homo sapiens
-
IC50: 0.0015 mM
0.0018
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]guanidine bis-(trifluoroacetate)
Homo sapiens
-
IC50: 0.0018 mM
0.0074
N-[3-amino-1-(S)-(1-piperidinylcarbonyl)propyl]guanidine bis-(trifluoroacetate)
Homo sapiens
-
IC50: 0.0074 mM
0.0074
N-[3-amino-4-oxo-4-(1-piperidinyl)butyl]benzamide
Homo sapiens
-
IC50: 0.0074 mM
0.0537
N1 -(2-cyclopentylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
Homo sapiens
-
IC50: 0.0537 mM, low inhibitory potential against EC 3.4.14.5
0.00000066
N1 -(4-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.66 nM
0.0000042
N1,N1-di(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 4.2 nM
0.0000006
N1,N1-dibenzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.6 nM
0.00000131
N1-(1-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.31 nM
0.0000027
N1-(2,4-dimethoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 2.7 nM
0.00000023
N1-(2-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.23 nM
0.0546
N1-(2-cyclohexylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
Homo sapiens
-
IC50: 0.0546 mM, low inhibitory potential against EC 3.4.14.5
0.00000185
N1-(2-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.85 nM
0.0000011
N1-(2-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.1 nM
0.00000134
N1-(3-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.34 nM
0.00000177
N1-(3-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.77 nM
0.0235
N1-(3-nitro-2-pyridinyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.0235 mM
0.00000048
N1-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.48 nM
0.00000022
N1-(4-chlorobenzyl)-N1-methyl-4-oxo-4-(1-piperidinyl)-1,3-butanediamine
Homo sapiens
-
IC50: 0.22 nm
0.0000011
N1-(cyclohexylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 1.1 nM
0.00000203
N1-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 2.03 nM
0.0000186
N1-cyclohexyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 18.6 nM
0.00000039
N1-[2-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.39 nM
0.00000041
N1-[4-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens
-
IC50: 0.41 nM
0.0038
N2-cyclopentyl-N-[(diphenoxyphosphoryl)(phenyl)methyl]glycinamide
Homo sapiens
-
-
0.0125
N3-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrobromide
Homo sapiens
-
IC50: 0.0125 mM
0.00000048
[(1R)-1-[[(2S)-2,4-diaminobutanoyl]amino]pentyl]boronic acid
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.3
-
purified enzyme, substrate Ala-Ala-2-nitroanilide
0.803
-
-
1.05
-
purified enzyme, substrate Lys-Ala-7-amido-4-methylcoumarin
2.11
-
purified enzyme, substrate Gly-Pro-7-amido-4-methylcoumarin
2.85
-
purified enzyme, substrate Ala-Pro-amino-4-trifluoromethylcoumarin
28
-
-
70
-
substrate: Lys-Ala-p-nitroanilide
additional information
-
-
additional information
-
sensitive and specific assay
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.2 - 5.3
-
hydrolysis of tripeptides
4.5
-
hydrolysis of (Ala)3
4.5
-
hydrolysis of tripeptides
4.5
-
hydrolysis of tripeptides
5
-
substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide
5 - 5.5
-
enzyme from seminal fluid
5 - 5.7
-
hydrolysis of 2-naphthylamide derivatives
5 - 6
-
enzyme from ovary
5 - 6
-
-
5.3
-
enzyme from kidney
5.3
-
hydrolysis of Lys-Ala-4-methylcoumarinamide
5.5
-
-
5.5
-
assay at
5.5
-
assay at
5.5
-
with substrate Lys-Ala-7-amido-4-methylcoumarin
5.5
-
enzyme from placenta
5.5
-
enzyme from spleen and skeletal muscle
5.5
-
hydrolysis of Gly-Pro-4-nitroanilide or Arg-Pro-4-nitroanilide
5.5
-
hydrolysis of arylamides
5.5
-
hydrolysis of Lys-Ala-4-methyoxy-2-naphthylamine or Lys-Ala-2-naphthylamide
5.5
-
hydrolysis of Lys-Ala-4-methyoxy-2-naphthylamine or Lys-Ala-2-naphthylamide
5.5
-
hydrolysis of Lys-Ala-2-naphthylamide
5.5
-
hydrolysis of dipeptide arylamides or dipeptide esters
5.5 - 6
-
-
6
-
enzyme from seminal plasma
7 - 7.5
-
hydrolysis of Ala-2-naphthylamide
7.5 - 8.2
-
hydrolysis of Gly-Pro-2-naphthylamide or Gly-Pro-2-(4-methoxy)-naphthylamide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.5 - 11.5
-
-
3.5 - 8.5
-
-
3.7 - 5.8
-
pH 3.5: about 85% of maximal activity, pH 5.8: about 45% of maximal activity
4 - 6
-
pH 4.0: about 50% of maximal activity, pH 6.0: about 40% of maximal activity, substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide
4 - 7
-
50% of maximal activity at pH 4 and 7
5.5 - 7
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5 - 4.8
-
isoelectric focusing
4.8
-
isoelectric focusing, enzyme from spleen and ovary
6.1
-
isoelectric focusing, enzyme from seminal plasma
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
highest activity in the hypothalamus
Manually annotated by BRENDA team
-
mRNA expression in ipsilateral and contralateral cortices. Dipeptidyl peptidase II and aminopeptidase N are up-regulated ipsilaterally from 6 h to 7 days post ischemia
Manually annotated by BRENDA team
Streptomyces sp. WM-23
-
-
-
Manually annotated by BRENDA team
-
of renal convoluted tubules, of uterine glands
Manually annotated by BRENDA team
-
in epithelial cells of convoluted tubuls, no activity in the glomeruli
Manually annotated by BRENDA team
-
of patients with Sjorgren's syndrome and healthy subjects
Manually annotated by BRENDA team
-
low activity, also alveolar macrophages
Manually annotated by BRENDA team
-
low activity, alveolar, myometrical and of the red pulp
Manually annotated by BRENDA team
-
smooth muscle cells
Manually annotated by BRENDA team
-
enzyme activity of prepuberal gilts rises with the onset of estrus, again just prior to ovulation on day 21, and during pregnancy. The activity is markedly elevated during the luteal and proestrous phases compared to the postovulatory phase. The highest specific activities occur at the proestrus when the follicle wall is undergoing breakdown
Manually annotated by BRENDA team
-
increased activity in serum of cancer patients
Manually annotated by BRENDA team
-
uterine myometrium
Manually annotated by BRENDA team
-
low activity, myometrium, myometrial macrophages, epithelium
Manually annotated by BRENDA team
-
peritoneal, 10-20fold higher activity as compared with macrophages
Manually annotated by BRENDA team
additional information
-
tissue distribution, overview
Manually annotated by BRENDA team
additional information
-
the enzyme is largely cell-associated during exponential growth in batch culture, but is progressively released by the bacteria before the cells enter stationary phase. The enzyme is completely cell-bound during growth in continuous culture
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Streptomyces sp. WM-23
-
-
-
-
Manually annotated by BRENDA team
-
compartmentalization of DPP II activity within lysosomal dense bodies that are concentrated primarily in the equatorial and sutural regions
Manually annotated by BRENDA team
-
enzyme form DPP II-S
Manually annotated by BRENDA team
-
enzyme form DPP II-M
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
58000
-
SDS-PAGE
678684
58000
-
SDS-PAGE, subunit enzyme from skeletal muscle and seminal plasma
679288
98000
-
gel filtration
36193
98000
-
gel filtration, enzyme from spleen
679288
105000
-
gel filtration and native PAGE
651779
110000
-
gel filtration, enzyme from kidney
679288
110000
-
gel filtration, enzyme from ovary
679288
110000
-
gel filtration
95229
116000
-
SDS-PAGE
679412
120000
-
gel filtration
667493
120000
-
gel filtration, enzyme from seminal fluid
679288
120000
-
gel filtration
679288
130000
-
gel filtration
679288
130000
-
gel filtration
95238
130000
-
gel filtration
95242
135000
-
gel filtration
95231
150000
-
enzyme from kidney cortex, in complex with adenosine deaminase, gel filtration
696370
160000
-
gel filtration
95228
185000
-
gel filtration
95244
200000
-
enzyme from lung, in complex with adenosine deaminase, gel filtration
696370
200000
-
non-denaturing PAGE
95244
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 37000, SDS-PAGE
?
Streptomyces sp. WM-23
-
x * 37000, SDS-PAGE
-
dimer
-
2 * 50000, SDS-PAGE
dimer
-
2 * 54054, mass spectrometry
dimer
-
2 * 60000, SDS-PAGE in presence of 2-mercaptoethanol
dimer
-
-
dimer
-
2 * 80000, SDS-PAGE
dimer
-
2 * 52500, SDS-PAGE
dimer
-
2 * 55000, SDS-PAGE
dimer
-
2 * 64000-66000, SDS-PAGE
dimer
-
2 * 54000, SDS-PAGE
dimer
-
2 * 64000, SDS-PAGE
homodimer
-
2 * 55000, gel filtration, enzyme from kidney; 2 * 60000, gel filtration, enzyme from seminal fluid
homodimer
-
2 * 60000, gel filtration
homodimer
-
2 * 58000, SDS-PAGE
homodimer
-
2 * 54000, enzyme from kidney cortex, SDS-PAGE; 2 * 60000, enzyme from lung, SDS-PAGE
trimer
-
3 * 58000, SDS-PAGE in absence of 2-mercaptoethanol; 3 * 61000, SDS-PAGE in presence of 2-mercaptoethanol
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
glycosylation is necessary for enzyme activity
glycoprotein
-
-
glycoprotein
-
glycosylation is necessary for enzyme activity
side-chain modification
-
contains glucosamine but not galactosamine; glycoprotein
side-chain modification
-
contains 2% carbohydrate, primarily mannose; glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3
-
4C, 24 h, loss of activity of DPP II-M
95229
3.5 - 10
-
4C, 24 h, stable
95244
4
-
25C, 24 h, 50% loss of activity
95233
4 - 8
-
4C, 24 h, DPP II-M is stable
95229
5 - 7
-
25C, 72 h, stable
95233
5 - 7.5
-
stable
95246
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
2 h, 30% loss of activity without a stabilizer. 10% loss of activity after 4 h in presence of 1 mg/ml bovine serum albumin or 0.1% Tween 20
667493
50
-
30 min, stable up to
651779
50
-
stable up to 50C
679288
50
-
stable
95246
55
-
30 min, stable up to
95244
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
in presence of stabilizing agents, the enzyme tolerates at least five subsequent freeze/thaw cycles
-
enzyme is stable during several repeated cycles of freezing and thawing
-
resistant against several repeated cycles of freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, -20C, or -80C, purified enzyme is stable for at least 4 months in presence of 1 mg/ml bovine serum albumin
-
-20C, pH 6.0, 20 mM BisTris-HCl buffer, stable
-
-20C, stable for at least 3 months
-
-30C, 20 mM sodium phosphate-buffered saline, pH 8.0, stable for at least several months
-
-30C, stable for at least several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complexed with adenosine deaminase or dipeptidyl peptidase IV, DEAE cellulose column chromatography, DEAE-Sephadex A-50 gel filtration, Sephadex G-25 gel filtration, and Sephadex G-200 gel filtration
-
partial, multiple isoforms
-
from placenta
-
HisBind Resin column chromatography
-
purification scheme includes an inhibitor-based affinity chromatographic step
-
131.3fold to homogeneity, from kidney
-
enzyme form DPP II-S and DPP II-M
-
ammonium sulfate precipitation and anion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in 293-EBNA cells
-
DNA sequence determination and analysis, sequence contains a leucine zipper motif
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
the enzyme is a potential urinary marker for the biochemical detection of kidney damage
medicine
-
mRNA expression in ipsilateral and contralateral cortices. Dipeptidyl peptidase II and aminopeptidase N are up-regulated ipsilaterally from 6 h to 7 days post ischemia. IPC1755, a non-selective protease inhibitor, reveals a significant reduction of cortical lesions after transient cerebral ischemia