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Literature summary for 3.4.14.2 extracted from
- Purification, molecular cloning, and immunohistochemical localization of dipeptidyl peptidase II from the rat kidney and its identity with quiescent cell proline dipeptidase (2001), J. Biochem., 129, 279-288.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, sequence contains a leucine zipper motif | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is stable during several repeated cycles of freezing and thawing | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-(2-aminoethyl)benzenesulfonyl fluoride | 60% inhibition at 1 mM, preincubation at room temperature for 15 min | Rattus norvegicus |  |
| benzamidine | 10% inhibition at 1 mM, preincubation at room temperature for 15 min | Rattus norvegicus | |
| Cu2+ | 40% inhibition at 1 mM, preincubation at room temperature for 15 min | Rattus norvegicus | |
| diisopropylfluorophosphate | complete inhibition at 1 mM, 25% inhibition at 0.1 mM, preincubation at room temperature for 15 min | Rattus norvegicus | |
| Hg2+ | complete inhibition at 1 mM, preincubation at room temperature for 15 min | Rattus norvegicus | |
| additional information | poor or no inhibition by EDTA, NEM, E-64, diprotin A, and actinonin | Rattus norvegicus | |
| PCMBS | 77% inhibition at 1 mM, preincubation at room temperature for 15 min | Rattus norvegicus | |
| PMSF | 45% inhibition at 1 mM, preincubation at room temperature for 15 min | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Rattus norvegicus | 5764 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54054 | - |
2 * 54054, mass spectrometry | Rattus norvegicus |
| 105000 | - |
gel filtration and native PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 131.3fold to homogeneity, from kidney | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aorta | - |
Rattus norvegicus | - |
| brain | - |
Rattus norvegicus | - |
| cerebellum | - |
Rattus norvegicus | - |
| cerebrum | - |
Rattus norvegicus | - |
| epididymis | - |
Rattus norvegicus | - |
| esophagus | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| large intestine | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| additional information | tissue distribution, overview | Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
| pancreas | - |
Rattus norvegicus | - |
| prostate | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| small intestine | - |
Rattus norvegicus | - |
| spermatozoon | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| stomach | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
| thymus | - |
Rattus norvegicus | - |
| trachea | - |
Rattus norvegicus | - |
| urinary bladder | - |
Rattus norvegicus | - |
| uterus | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
purified enzyme, substrate Ala-Ala-2-nitroanilide | Rattus norvegicus |
| 1.05 | - |
purified enzyme, substrate Lys-Ala-7-amido-4-methylcoumarin | Rattus norvegicus |
| 2.11 | - |
purified enzyme, substrate Gly-Pro-7-amido-4-methylcoumarin | Rattus norvegicus |
| 2.85 | - |
purified enzyme, substrate Ala-Pro-amino-4-trifluoromethylcoumarin | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, 20 mM sodium phosphate-buffered saline, pH 8.0, stable for at least several months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-2-nitroanilide + H2O | low activity | Rattus norvegicus | Ala-Ala + 2-nitroaniline | - |
? | |
| Ala-Pro-amino-4-trifluoromethylcoumarin + H2O | - |
Rattus norvegicus | Ala-Pro + amino-4-trifluoromethylcoumarin | - |
? | |
| Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| Lys-Ala-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | Lys-Ala + 7-amino-4-methylcoumarin | - |
? | |
| additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 54054, mass spectrometry | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme belongs to the Pro-X carboxypeptidase family S28 | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, stable up to | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
with substrate Lys-Ala-7-amido-4-methylcoumarin | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 11.5 | - |
Rattus norvegicus |
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