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Literature summary for 3.4.14.2 extracted from

  • Maes, M.B.; Lambeir, A.M.; Gilany, K.; Senten, K.; Van der Veken, P.; Leiting, B.; Augustyns, K.; Scharpe, S.; De Meester, I.
    Kinetic investigation of human dipeptidyl peptidase II (DPPII)-mediated hydrolysis of dipeptide derivatives and its identification as quiescent cell proline dipeptidase (QPP)/dipeptidyl peptidase 7 (DPP7) (2005), Biochem. J., 386, 315-324.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.2

General Stability

General Stability Organism
in presence of stabilizing agents, the enzyme tolerates at least five subsequent freeze/thaw cycles Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
lysyl-piperidide
-
 Homo sapiens
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 Ala-Pro-p-nitroanilide 37°C, pH 7.0 Homo sapiens
0.019
-
 Lys-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.02
-
 Nle-Nle-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.0223
-
 His-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.04
-
 Lys-Ala-p-nitroanilide 37°C, pH 7.0 Homo sapiens
0.04
-
 Ala-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.04
-
 Arg-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.058
-
 Phe-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.07
-
 Gly-Pro-p-nitroanilide 37°C, pH 7-0 Homo sapiens
0.08
-
 Ser-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.083
-
 His-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.101
-
 Asn-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.21
-
 Gly-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.22
-
 Tyr-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens
0.57
-
 Lys-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens
1.376
-
 Pro-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
120000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UHL4
-
-

Purification (Commentary)

Purification (Comment) Organism
purification scheme includes an inhibitor-based affinity chromatographic step Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
seminal plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70
-
 substrate: Lys-Ala-p-nitroanilide Homo sapiens

Storage Stability

Storage Stability Organism
4°C, -20°C, or -80°C, purified enzyme is stable for at least 4 months in presence of 1 mg/ml bovine serum albumin Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ala-Pro + p-nitroaniline
-
 ?
Arg-Pro-p-nitroanilide + H2O
-
 Homo sapiens Arg-Pro + p-nitroaniline
-
 ?
Asn-Pro-p-nitroanilide + H2O
-
 Homo sapiens Asn-Pro + p-nitroaniline
-
 ?
Gly-Pro-p-nitroanilide + H2O
-
 Homo sapiens Gly-Pro + p-nitroaniline
-
 ?
His-Ala-p-nitroanilide + H2O
-
 Homo sapiens His-Ala + p-nitroaniline
-
 ?
His-Pro-p-nitroanilide + H2O
-
 Homo sapiens His-Pro + p-nitroaniline
-
 ?
Lys-Ala-p-nitroanilide + H2O
-
 Homo sapiens Lys-Ala + p-nitroaniline
-
 ?
Lys-Pro-p-nitroanilide + H2O
-
 Homo sapiens Lys-Pro + p-nitroaniline
-
 ?
Nle-Nle-p-nitroanilide + H2O
-
 Homo sapiens Nle-Nle + p-nitroaniline
-
 ?
Phe-Pro-p-nitroanilide + H2O
-
 Homo sapiens Phe-Pro + p-nitroaniline
-
 ?
Pro-Ala-p-nitroanilide + H2O
-
 Homo sapiens Pro-Ala + p-nitroaniline
-
 ?
Ser-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ser-Pro + p-nitroaniline
-
 ?
Tyr-Ala-p-nitroanilide + H2O
-
 Homo sapiens Tyr-Ala + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens

Synonyms

Synonyms Comment Organism
DPPII
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 2 h, 30% loss of activity without a stabilizer. 10% loss of activity after 4 h in presence of 1 mg/ml bovine serum albumin or 0.1% Tween 20 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 Lys-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
11
-
 Tyr-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens
12.8
-
 His-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens
15.3
-
 Pro-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens
18.4
-
 Nle-Nle-p-nitroanilide 37°C, pH 5.5 Homo sapiens
28.4
-
 His-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
65.8
-
 Arg-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
66
-
 Ser-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
67.3
-
 Gly-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
77.7
-
 Lys-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
89
-
 Ala-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
93
-
 Phe-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
105.4
-
 Ala-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
116.9
-
 Ser-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
184.6
-
 Asn-Pro-p-nitroanilide 37°C, pH 5.5 Homo sapiens
229.3
-
 Lys-Ala-p-nitroanilide 37°C, pH 5.5 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 4.0: about 50% of maximal activity, pH 6.0: about 40% of maximal activity, substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000052
-
 lysyl-piperidide substrate: Lys-Ala-p-nitroanilide, pH 7.0, 37°C Homo sapiens
0.000063
-
 lysyl-piperidide substrate: Ala-Pro-p-nitroanilide, pH 7.0, 37°C Homo sapiens
0.00095
-
 N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride substrate: Lys-Ala-p-nitroanilide, pH 5.5, 37°C Homo sapiens
0.001
-
 N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride substrate: Lys-Ala-p-nitroanilide, pH 7.0, 37°C Homo sapiens
0.00105
-
 N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride substrate: Ala-Pro-p-nitroanilide, pH 7.0, 37°C Homo sapiens
0.00145
-
 N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride substrate: Ala-Pro-p-nitroanilide, pH 5.5, 37°C Homo sapiens
0.00172
-
 lysyl-piperidide substrate: Lys-Ala-p-nitroanilide, pH 5.5, 37°C Homo sapiens
0.00264
-
 lysyl-piperidide substrate: Ala-Pro-p-nitroanilide, pH 5., 37°C Homo sapiens