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Literature summary for 3.4.14.2 extracted from

  • Huang, K.; Takagaki, M.; Kani, K.; Ohkubo, I.
    Dipeptidyl peptidase II from porcine seminal plasma: purification, characterization, and its homology to granzymes, cytosolic cell proteinases (CCP 1-4) (1996), Biochim. Biophys. Acta, 1290, 149-156.
    View publication on PubMed
Search for more literature for 3.4.14.2

General Stability

General Stability Organism
resistant against several repeated cycles of freezing and thawing Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)benzenesulfonyl fluoride
-
 Sus scrofa
Cu2+
-
 Sus scrofa
diisopropyl fluorophosphate
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 L-Lys-L-Ala-7-amido-4-methylcoumarin
-
 Sus scrofa
1.33
-
 Gly-Pro-4-methylcoumarin 7-amide
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 3 * 58000, SDS-PAGE in absence of 2-mercaptoethanol Sus scrofa
61000
-
 3 * 61000, SDS-PAGE in presence of 2-mercaptoethanol Sus scrofa
185000
-
 gel filtration Sus scrofa
200000
-
 non-denaturing PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
seminal plasma
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.803
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-30°C, stable for at least several months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Pro-2-(4-methoxy)-naphthylamide + H2O
-
 Sus scrofa ?
-
 ?
Gly-Pro-4-methylcoumarin 7-amide + H2O
-
 Sus scrofa Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
L-Lys-L-Ala-7-amido-4-methylcoumarin + H2O
-
 Sus scrofa L-Lys-L-Ala + 7-amino-4-methylcoumarin
-
 ?
Lys-Ala-2-(4-methoxy)-naphthylamide Lys-Ala-4-methoxy-2-naphthylamide + H2O
-
 Sus scrofa ?
-
 ?
Lys-Ala-4-methylcoumaryl-7-amide + H2O
-
 Sus scrofa Lys-Ala + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 58000, SDS-PAGE in absence of 2-mercaptoethanol Sus scrofa
trimer 3 * 61000, SDS-PAGE in presence of 2-mercaptoethanol Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, stable up to Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 10 4°C, 24 h, stable Sus scrofa