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Literature summary for 3.4.14.2 extracted from

  • Eisenhauer, D.A.; McDonald, J.K.
    A novel dipeptidyl peptidase II from the porcine ovary. Purification and characterization of a lysosomal serine protease showing enhanced specificity for prolyl bonds (1986), J. Biol. Chem., 261, 8859-8865.
    View publication on PubMed
Search for more literature for 3.4.14.2

Inhibitors

Inhibitors Comment Organism Structure
4-Chloromercuriphenylsulfonate
-
 Sus scrofa
diisopropyl fluorophosphate
-
 Sus scrofa
Hg2+ HgCl2 Sus scrofa
iodoacetate weak Sus scrofa
Na+ 100 mM NaCl, 11% inhibition Sus scrofa
p-Nitrophenyl-p-guanidinobenzoate
-
 Sus scrofa
pepstatin 0.01 mM, 11% inhibition Sus scrofa
phenylmethylsulfonyl fluoride
-
 Sus scrofa
puromycin 1 mM, 47% inhibition Sus scrofa
Tris 50 mM, 55% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0086
-
 Arg-Pro-2-naphthylamide
-
 Sus scrofa
0.045
-
 Phe-Pro-2-naphthylamide
-
 Sus scrofa
0.099
-
 Lys-Ala-2-naphthylamide
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Sus scrofa 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 2 * 54000, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Sus scrofa
side-chain modification contains 2% carbohydrate, primarily mannose Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
ovary
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ala)3 + H2O
-
 Sus scrofa ?
-
 ?
Arg-Ala-2-naphthylamide + H2O 11% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Arg-Ala + 2-naphthylamine
-
 ?
Arg-Pro-2-naphthylamide + H2O 27% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Arg-Pro + 2-naphthylamine
-
 ?
Gly-Pro-Ala + H2O
-
 Sus scrofa Gly-Pro + Ala
-
 ?
Lys-Ala-2-naphthylamide + H2O 15% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Lys-Ala + 2-naphthylamine
-
 ?
Lys-Ala-Ala + H2O
-
 Sus scrofa Lys-Ala + Ala
-
 ?
Lys-Pro-2-naphthylamide + H2O 46% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Lys-Pro + 2-naphthylamine
-
 ?
Phe-Pro-2-naphthylamide + H2O
-
 Sus scrofa Phe-Pro + 2-naphthylamine
-
 ?
Phe-Pro-Ala + H2O
-
 Sus scrofa Phe-Pro + Ala
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 Lys-Ala-2-naphthylamide
-
 Sus scrofa
6.4
-
 Arg-Pro-2-naphthylamide
-
 Sus scrofa
54
-
 Phe-Pro-2-naphthylamide
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 25°C, 24 h, 50% loss of activity Sus scrofa
5 7 25°C, 72 h, stable Sus scrofa