Information on EC 3.2.1.58 - glucan 1,3-beta-glucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.58
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,3-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
3-beta-D-glucan glucohydrolase
Acts on oligosaccharides, but very slowly on laminaribiose.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-49-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain C59
-
-
Manually annotated by BRENDA team
strain C59
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain RIB40
Uniprot
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain CT2
-
-
Manually annotated by BRENDA team
strain CT2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1
UniProt
Manually annotated by BRENDA team
Fungi imperfecti
-
-
-
Manually annotated by BRENDA team
Geotrichum lactis
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Kluyveromyces phaseolosporus
-
-
-
Manually annotated by BRENDA team
strain X-33
-
-
Manually annotated by BRENDA team
strain X-33
-
-
Manually annotated by BRENDA team
gene exg2
-
-
Manually annotated by BRENDA team
gene exg2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica
strain Po1h
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica Po1h
strain Po1h
-
-
Manually annotated by BRENDA team
strain IB-X-b
-
-
Manually annotated by BRENDA team
strain IB-X-b
-
-
Manually annotated by BRENDA team
gene exgP
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
member of glycosyl hydrolase family 5
-
-
Manually annotated by BRENDA team
isolated from a commercial source Kitarase-M
-
-
Manually annotated by BRENDA team
cf. EC 3.2.1.21
UniProt
Manually annotated by BRENDA team
var. versatilis
-
-
Manually annotated by BRENDA team
sea urchin
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain K
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucose
show the reaction diagram
18% of the activity with 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
show the reaction diagram
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
2.7% of the activity with 4-nitrophenyl alpha-D-glucopyranoside
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
-
3.9% of the activity with 4-nitrophenyl alpha-D-glucopyranoside
-
?
4-nitrophenyl beta-D-cellobiopyranoside + H2O
4-nitrophenol + D-cellobiose
show the reaction diagram
3% of the activity with 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
9% of the activity with 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
4-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
show the reaction diagram
4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosaminide
show the reaction diagram
-
-
-
-
?
amygdalin + H2O
?
show the reaction diagram
amygdalin + H2O
D-glucose + ?
show the reaction diagram
63% of the activity with laminarin
-
-
?
arbutin + H2O
D-glucose + ?
show the reaction diagram
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
barley beta-(1->3)-glucan + H2O
?
show the reaction diagram
barley beta-glucan + H2O
D-glucose + ?
show the reaction diagram
beta-1,3-glucan + H2O
?
show the reaction diagram
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylpachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
cell wall of Rhizoctania solani + H2O
?
show the reaction diagram
-
-
-
?
cellobiose + H2O
2 D-glucose
show the reaction diagram
cellobiose + H2O
?
show the reaction diagram
-
3% of activity compared to laminaran
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
cellodextrin + H2O
D-glucose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
D-glucose + ?
show the reaction diagram
cellotriose + H2O
?
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
D-glucose + ?
show the reaction diagram
-
67% of the activity with laminaribiose
-
?
CM-cellulose + H2O
D-glucose
show the reaction diagram
-
very weak hydrolysis
-
?
CM-pachyman + H2O
D-glucose
show the reaction diagram
curdlan + H2O
?
show the reaction diagram
curdlan + H2O
D-glucose + ?
show the reaction diagram
14% of the activity with laminarin from Laminaria digitata
-
-
?
esculin + H2O
?
show the reaction diagram
esculin + H2O
D-glucose + 4-hydroxyphenol
show the reaction diagram
gentiobiose + H2O
?
show the reaction diagram
-
21% of activity compared to laminaran
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
gentobiose + H2O
2 D-glucose
show the reaction diagram
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
laminaran + H2O
?
show the reaction diagram
-
-
-
-
?
laminaran + H2O
D-glucose
show the reaction diagram
-
at pH 5.0, strictly specific for laminaran and laminarioligosaccharides, with a strict exo mode of action, with glucose being the sole reaction product even at the initial stages of hydrolysis
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
laminaribiose
6-O-glucosyl-laminaribiose
show the reaction diagram
-
transglycosylation
-
-
?
laminaribiose + H2O
2 D-glucose
show the reaction diagram
laminaribiose + H2O
?
show the reaction diagram
laminaribiose + H2O
D-glucose
show the reaction diagram
laminariheptaose + H2O
?
show the reaction diagram
laminarihexaose + 4 H2O
4 D-glucose + laminaribiose
show the reaction diagram
laminarihexaose + 5 H2O
6 D-glucose
show the reaction diagram
laminarihexaose + H2O
?
show the reaction diagram
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
laminarin + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
beta-D-glucose + laminaribiose
show the reaction diagram
-
61% beta-D-glucose + 39% laminaribiose
-
-
?
laminarin + H2O
D-glucose
show the reaction diagram
laminarin + H2O
D-glucose + ?
show the reaction diagram
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
laminarin + H2O
glycerol + glycoaldehyde + D-glucose
show the reaction diagram
laminaripentaose + 3 H2O
3 D-glucose + laminaribiose
show the reaction diagram
laminaripentaose + 4 H2O
4 D-glucose
show the reaction diagram
-
-
-
?
laminaripentaose + H2O
?
show the reaction diagram
laminaripentaose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
laminaritetraose + 2 H2O
2 D-glucose + laminaribiose
show the reaction diagram
laminaritetraose + 3 H2O
4 D-glucose
show the reaction diagram
laminaritetraose + H2O
?
show the reaction diagram
laminaritetraose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
laminaritriose + 2 H2O
3 D-glucose
show the reaction diagram
laminaritriose + H2O
?
show the reaction diagram
laminaritriose + H2O
D-glucose + ?
show the reaction diagram
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
lentinan + H2O
?
show the reaction diagram
lichenan + H2O
?
show the reaction diagram
lichenin + H2O
D-glucose
show the reaction diagram
linear alpha-1,5-L-arabinan + H2O
?
show the reaction diagram
luteose + H2O
D-glucose
show the reaction diagram
-
-
-
?
maltose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
N,N'-diacetyl-chitobiose + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-galactosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-glucosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
neutral glucan + H2O
D-glucose + laminaribiose
show the reaction diagram
-
from Achlya
-
?
nigeran + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-glucopyranoside + H2O
o-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
oligoglucosides from pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
22% of activity compared to laminaran
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
paramylon + H2O
D-glucose
show the reaction diagram
-
-
-
?
pendulan + H2O
D-glucose
show the reaction diagram
-
-
-
?
phosphoglucan + H2O
?
show the reaction diagram
-
-
-
-
?
polydatin + H2O
D-glucose + 3,4',5-trihydroxystilbene
show the reaction diagram
-
-
-
?
pullulan + H2O
?
show the reaction diagram
-
-
-
-
?
purified cell wall from Rhizoctonia solani + H2O
?
show the reaction diagram
-
25% of activity compared to laminarin
-
-
?
pustulan + H2O
?
show the reaction diagram
pustulan + H2O
D-glucose
show the reaction diagram
salicin + H2O
?
show the reaction diagram
salicin + H2O
D-glucose + 2-hydroxymethylphenol
show the reaction diagram
-
-
-
?
schizophyllan + H2O
D-glucose + gentiobiose
show the reaction diagram
scleroglucan + H2O
?
show the reaction diagram
-
-
-
-
?
sclerotan + H2O
D-glucose + gentiobiose
show the reaction diagram
sophorose + H2O
2 beta-D-glucose
show the reaction diagram
-
-
-
-
?
starch + H2O
?
show the reaction diagram
-
-
-
?
sugar beet arabinan + H2O
?
show the reaction diagram
wheat flour arabinoxylan + H2O
?
show the reaction diagram
zymosan A + H2O
D-glucose + ?
show the reaction diagram
-
45% of the activity with laminarin
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-1,3-glucan + H2O
?
show the reaction diagram
P29717
-
-
-
?
cell wall of Rhizoctania solani + H2O
?
show the reaction diagram
A9YZW0
-
-
-
?
laminarin + H2O
?
show the reaction diagram
laminarin + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
-
?
lentinan + H2O
?
show the reaction diagram
starch + H2O
?
show the reaction diagram
A9YZW0
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AgNO3
Fungi imperfecti
-
activity enhanced by 15%
Co2+
-
enhanced activity
Cu2+
-
10 mM, 82% residual activity
Li+
-
activates
Mg2+
-
enhanced activity
Ni2+
-
activates
Zn2+
-
enhanced activity
additional information
-
no effect by 5 mM of Mg2+, Ca2+, Na+, K+, or Li+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2MoO4
-
-
1,10-phenanthroline
1-deoxynojirimycin
-
-
2-deoxy-D-glucose
-
slight
2-fluoroglucosylpyranoside
-
mechanism-based
5-Amino-1H-tetrazole
-
diazotized
AgNO3
-
-
Ammonium molybdate
-
-
CoSO4
Fungi imperfecti
-
-
CuCl2
CuSO4
D-glucono-delta-lactone
ethanol
Fungi imperfecti
-
inhibition reversible when up to 80% ethanol concentration used
FeCl2
-
-
FeCl3
glucono-delta-lactone
-
-
HgCl2
indole-3-acetic acid
-
slight
iodoacetic acid
KMnO4
L-cysteine
-
10 mM, 68% residual activity
Laminaran
-
strong at high concentrations
Li2SO4
Fungi imperfecti
-
-
LL-37
-
antimicrobial peptide derived from human cathelicidin, by proteinase-3 cleavage. LL-37 significantly reduces Candida albicans adhesion to plastic, oral epidermoid OECM-1 cells, and urinary bladders of female BALB/c mice. LL-37 reduces Xog1 activity and thus interrupts cell-wall remodeling
-
MnCl2
N-bromosuccinimide
Na2HAsO4
Fungi imperfecti
-
-
Ni2+
-
83.5% inhibition at 5 mM
Nojirimycin
-
-
p-chloromercuribenzoate
Fungi imperfecti
-
-
p-hydroxymercuribenzoate
-
-
periodate-oxidized and borohydride reduced laminarin
-
substrate inhibition
-
Phenyl mercuric nitrate
-
-
phenylglyoxal hydrate
-
10 mM, 86% residual activity
potassium metabisulfite
1 mM, 90% of initial activity
SDS
-
34% inhibition at 5 mM
Tetranitromethane
-
-
ZnSO4
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 50°C
1.4
4-nitrophenyl beta-D-glucoside
-
1.5
4-nitrophenyl-alpha-L-arabinofuranoside
pH 6.0, 40°C
2
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37°C
1.9
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37°C
5
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37°C
16.5
beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37°C
2.1
beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37°C
0.095 - 3.7
cellobiose
0.92
Cellotetraose
pH 5.0, 50°C
1.5 - 2
cellotriose
pH 5.0, 50°C
11.1
gentiobiose
-
pH 5.5, 30°C
4.15
gentobiose
pH 5.0, 50°C
1.03 - 13
Laminaran
0.255 - 34.1
laminaribiose
0.36 - 0.83
Laminarihexaose
0.0018 - 0.85
Laminarin
0.0119 - 32
Laminaripentaose
0.06 - 20.9
laminaritetraose
0.223 - 28.3
laminaritriose
1.9 - 2
methylumbelliferyl-beta-glucoside
0.41 - 6.6
p-nitrophenyl-beta-D-glucopyranoside
2.82 - 5
p-nitrophenyl-beta-D-glucoside
0.37 - 24.8
pustulan
-
additional information
Laminaran
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.071
4-nitrophenyl beta-D-glucopyranoside
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
123.6
4-nitrophenyl-alpha-L-arabinofuranoside
Cellulomonas fimi
F4H166
pH 6.0, 40°C
0.043
cellobiose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
0.059
Cellotetraose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
0.067
cellotriose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
0.056
gentobiose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
0.033 - 29550
Laminaran
0.078 - 45.6
laminaribiose
0.05 - 157
Laminarin
1.53 - 33.7
Laminaripentaose
2.3 - 39
laminaritetraose
0.077 - 41.8
laminaritriose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.42
4-nitrophenyl beta-D-glucopyranoside
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
510
0.012
cellobiose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
82
0.063
Cellotetraose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
590
0.044
cellotriose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
516
0.013
gentobiose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
32231
0.014
Laminaran
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
8128
0.062
laminaribiose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
875
0.033
laminaritriose
Rhizomucor miehei
A0A0B4RUW2
pH 5.0, 50°C
1875
additional information
Laminarin
Candida albicans
-
kcat/KM value of wild-type is 32.7 mg per ml and sec, pH 5.6, 37°C
461
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
D-glucono-delta-lactone
-
-
1.25
glucono-delta-lactone
-
pH 5.0, 50°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.004
growth on chitin
0.005
growth on cellulose
0.006
growth on chitosan
0.008
growth on glucose
0.02
extracellular enzyme, substrate starch; growth on starch
0.265
-
purified native enzyme
0.7
-
isoenzyme I
2.5
-
isoenzyme II
3 - 8
-
purified enzyme
4.7
isoenzyme I
7.75
-
pH 5.0, 40°C
11.7
-
purified mature enzyme
12.8
isoenzyme II
13.77
-
substrate: 4-nitrophenyl-beta-D-glucopyranoside
19.1
substrate laminaran, pH 5.0, 50°C
19.4
substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 6.0, 40°C
62
-
crude enzyme from wild-type strain WC91-2
63
-
pH 4.5, 37°C
169.4
Fungi imperfecti
-
-
249
-
crude recombinant enzyme from overexpressing Yarrowia lipolytica strain Po1h
261
-
enzyme I
292
-
enzyme II
335
-
enzyme III
511
-
purified recombinant enzyme
592
-
pH 5.0, 50°C
818
substrate laminaritriose, pH 6.0, 37°C
1424
substrate laminaritetraose, pH 6.0, 37°C
1640
substrate laminarihexaose, pH 6.0, 37°C
1683
substrate laminaripentaose, pH 6.0, 37°C
1966
-
purified native enzyme
2848
substrate laminarihexaose, pH 6.0, 37°C
3102
substrate laminaripentaose, pH 6.0, 37°C
3241
substrate laminaritetraose, pH 6.0, 37°C
3813
substrate laminaritriose, pH 6.0, 37°C
4481
substrate laminaritriose, pH 6.0, 37°C
additional information
-
44 U/mg. One unit of beta-1,3-glucanase activity is defined as the amount of the enzyme that releases reducing saccharides equivalent to 0.001 mg glucose per minute
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2
-
assay at
4.4 - 4.5
-
-
4.5 - 5
4.7 - 5.2
-
-
5.5 - 6
-
-
6.5
-
enzyme I
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.8 - 8
-
more than 50% of maximum activity within
3 - 6.5
3 - 7
-
activity range, profile, overview
4.5 - 7
-
enzyme II and III
5 - 7.5
-
enzyme I
5
58% of maximum activity
7
72% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
activity range
30 - 55
-
activity range
30 - 80
-
30°C: about 40% of maximal activity, 80°C: about 65% of maximal activity
30 - 60
-
activity range, profile, overview
35 - 67
-
35°C: about 70% of maximal activity, 67°C: about 85% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3
-
isoelectric focusing
6
calculated from amino acid sequence
7.1
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
growth on laminarin from Laminaria digitata or beta-glucan from the fruiting bodies of Pleurotus cornucopiae
Manually annotated by BRENDA team
-
from Aspergillus saitoi
Manually annotated by BRENDA team
additional information