Information on EC 3.2.1.58 - glucan 1,3-beta-glucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.58
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,3-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
3-beta-D-glucan glucohydrolase
Acts on oligosaccharides, but very slowly on laminaribiose.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-49-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain C59
-
-
Manually annotated by BRENDA team
strain C59
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain RIB40
Uniprot
Manually annotated by BRENDA team
Basidiomycota QM806
QM806
-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain CT2
-
-
Manually annotated by BRENDA team
strain CT2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1
UniProt
Manually annotated by BRENDA team
Fungi imperfecti
-
-
-
Manually annotated by BRENDA team
Geotrichum lactis
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Kluyveromyces phaseolosporus
-
-
-
Manually annotated by BRENDA team
strain X-33
-
-
Manually annotated by BRENDA team
strain X-33
-
-
Manually annotated by BRENDA team
gene exg2
-
-
Manually annotated by BRENDA team
gene exg2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica
strain Po1h
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica Po1h
strain Po1h
-
-
Manually annotated by BRENDA team
strain IB-X-b
-
-
Manually annotated by BRENDA team
strain IB-X-b
-
-
Manually annotated by BRENDA team
gene exgP
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pyrenophora tritici-repentis race 5
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
member of glycosyl hydrolase family 5
-
-
Manually annotated by BRENDA team
isolated from a commercial source Kitarase-M
-
-
Manually annotated by BRENDA team
var. versatilis
-
-
Manually annotated by BRENDA team
sea urchin
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain K
-
-
Manually annotated by BRENDA team
NCYC 434
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
show the reaction diagram
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosaminide
show the reaction diagram
-
-
-
-
?
amygdalin + H2O
?
show the reaction diagram
barley beta-(1->3)-glucan + H2O
?
show the reaction diagram
beta-1,3-glucan + H2O
?
show the reaction diagram
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylpachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
cell wall of Rhizoctania solani + H2O
?
show the reaction diagram
-
-
-
?
cellobiose + H2O
?
show the reaction diagram
-
3% of activity compared to laminaran
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
cellodextrin + H2O
D-glucose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
D-glucose + ?
show the reaction diagram
cellotriose + H2O
?
show the reaction diagram
-
-
-
-
?
CM-cellulose + H2O
D-glucose
show the reaction diagram
-
very weak hydrolysis
-
?
CM-pachyman + H2O
D-glucose
show the reaction diagram
curdlan + H2O
?
show the reaction diagram
curdlan + H2O
D-glucose + ?
show the reaction diagram
14% of the activity with laminarin from Laminaria digitata
-
-
?
esculin + H2O
?
show the reaction diagram
gentiobiose + H2O
?
show the reaction diagram
-
21% of activity compared to laminaran
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
laminaran + H2O
?
show the reaction diagram
-
-
-
-
?
laminaran + H2O
D-glucose
show the reaction diagram
-
at pH 5.0, strictly specific for laminaran and laminarioligosaccharides, with a strict exo mode of action, with glucose being the sole reaction product even at the initial stages of hydrolysis
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
laminaribiose
6-O-glucosyl-laminaribiose
show the reaction diagram
-
transglycosylation
-
-
?
laminaribiose + H2O
?
show the reaction diagram
laminaribiose + H2O
D-glucose
show the reaction diagram
laminariheptaose + H2O
?
show the reaction diagram
laminarihexaose + H2O
?
show the reaction diagram
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
laminarin + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
beta-D-glucose + laminaribiose
show the reaction diagram
-
61% beta-D-glucose + 39% laminaribiose
-
-
?
laminarin + H2O
D-glucose
show the reaction diagram
laminarin + H2O
D-glucose + ?
show the reaction diagram
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
laminarin + H2O
glycerol + glycoaldehyde + D-glucose
show the reaction diagram
laminaripentaose + H2O
?
show the reaction diagram
laminaripentaose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
laminaritetraose + H2O
?
show the reaction diagram
laminaritetraose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
laminaritriose + H2O
?
show the reaction diagram
laminaritriose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
lentinan + H2O
?
show the reaction diagram
lichenan + H2O
?
show the reaction diagram
lichenin + H2O
D-glucose
show the reaction diagram
luteose + H2O
D-glucose
show the reaction diagram
-
-
-
?
maltose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
N,N'-diacetyl-chitobiose + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-galactosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-glucosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
neutral glucan + H2O
D-glucose + laminaribiose
show the reaction diagram
-
from Achlya
-
?
nigeran + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-glucopyranoside + H2O
o-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
oligoglucosides from pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
22% of activity compared to laminaran
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
paramylon + H2O
D-glucose
show the reaction diagram
-
-
-
?
pendulan + H2O
D-glucose
show the reaction diagram
-
-
-
?
phosphoglucan + H2O
?
show the reaction diagram
-
-
-
-
?
pullulan + H2O
?
show the reaction diagram
-
-
-
-
?
purified cell wall from Rhizoctonia solani + H2O
?
show the reaction diagram
-
25% of activity compared to laminarin
-
-
?
pustulan + H2O
?
show the reaction diagram
pustulan + H2O
D-glucose
show the reaction diagram
salicin + H2O
?
show the reaction diagram
schizophyllan + H2O
D-glucose + gentiobiose
show the reaction diagram
scleroglucan + H2O
?
show the reaction diagram
-
-
-
-
?
sclerotan + H2O
D-glucose + gentiobiose
show the reaction diagram
sophorose + H2O
2 beta-D-glucose
show the reaction diagram
-
-
-
-
?
starch + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-1,3-glucan + H2O
?
show the reaction diagram
P29717
-
-
-
?
cell wall of Rhizoctania solani + H2O
?
show the reaction diagram
A9YZW0
-
-
-
?
laminarin + H2O
?
show the reaction diagram
laminarin + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
-
?
lentinan + H2O
?
show the reaction diagram
starch + H2O
?
show the reaction diagram
A9YZW0
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AgNO3
Fungi imperfecti
-
activity enhanced by 15%
Co2+
-
enhanced activity
Cu2+
-
10 mM, 82% residual activity
Li+
-
activates
Mg2+
-
enhanced activity
Ni2+
-
activates
Zn2+
-
enhanced activity
additional information
-
no effect by 5 mM of Mg2+, Ca2+, Na+, K+, or Li+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2MoO4
-
-
1,10-phenanthroline
1-deoxynojirimycin
-
-
2-deoxy-D-glucose
-
slight
2-fluoroglucosylpyranoside
-
mechanism-based
5-Amino-1H-tetrazole
-
diazotized
AgNO3
-
-
Ammonium molybdate
-
-
CoSO4
Fungi imperfecti
-
-
D-glucono-delta-lactone
ethanol
Fungi imperfecti
-
inhibition reversible when up to 80% ethanol concentration used
FeCl2
-
-
glucono-delta-lactone
-
-
indole-3-acetic acid
-
slight
iodoacetic acid
L-cysteine
-
10 mM, 68% residual activity
Laminaran
-
strong at high concentrations
Li2SO4
Fungi imperfecti
-
-
LL-37
-
antimicrobial peptide derived from human cathelicidin, by proteinase-3 cleavage. LL-37 significantly reduces Candida albicans adhesion to plastic, oral epidermoid OECM-1 cells, and urinary bladders of female BALB/c mice. LL-37 reduces Xog1 activity and thus interrupts cell-wall remodeling
-
N-bromosuccinimide
Na2HAsO4
Fungi imperfecti
-
-
Ni2+
-
83.5% inhibition at 5 mM
Nojirimycin
-
-
p-chloromercuribenzoate
Fungi imperfecti
-
-
p-hydroxymercuribenzoate
-
-
periodate-oxidized and borohydride reduced laminarin
-
substrate inhibition
-
Phenyl mercuric nitrate
-
-
phenylglyoxal hydrate
-
10 mM, 86% residual activity
SDS
-
34% inhibition at 5 mM
Tetranitromethane
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
4-nitrophenyl beta-D-glucoside
-
2
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
1.9
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
5
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
16.5
beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
2.1
beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
0.095
cellobiose
-
-
11.1
gentiobiose
-
pH 5.5, 30C
1.03 - 13
Laminaran
0.255 - 34.1
laminaribiose
0.36 - 0.83
Laminarihexaose
0.0018 - 0.85
Laminarin
0.0119 - 32
Laminaripentaose
0.06 - 20.9
laminaritetraose
0.223 - 28.3
laminaritriose
1.9 - 2
methylumbelliferyl-beta-glucoside
0.41 - 6.6
p-nitrophenyl-beta-D-glucopyranoside
2.82 - 5
p-nitrophenyl-beta-D-glucoside
0.37 - 24.8
pustulan
additional information
Laminaran
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
29550
Laminaran
Rasamsonia emersonii
-
pH 5.0, 50C
33.3 - 45.6
laminaribiose
0.05 - 157
Laminarin
1.53 - 33.7
Laminaripentaose
2.3 - 39
laminaritetraose
41.8
laminaritriose
Phanerochaete chrysosporium
-
30C, pH 4.5, hydrolysis
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
Laminarin
Candida albicans
-
kcat/KM value of wild-type is 32.7 mg per ml and sec, pH 5.6, 37C
461
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
D-glucono-delta-lactone
-
-
1.25
glucono-delta-lactone
-
pH 5.0, 50C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.004
growth on chitin
0.005
growth on cellulose
0.006
growth on chitosan
0.008
growth on glucose
0.02
extracellular enzyme, substrate starch; growth on starch
0.265
-
purified native enzyme
0.7
-
isoenzyme I
2.5
-
isoenzyme II
3 - 8
-
purified enzyme
4.7
isoenzyme I
7.75
-
pH 5.0, 40C
11.7
-
purified mature enzyme
12.8
isoenzyme II
13.77
-
substrate: 4-nitrophenyl-beta-D-glucopyranoside
62
-
crude enzyme from wild-type strain WC91-2
63
-
pH 4.5, 37C
169.4
Fungi imperfecti
-
-
249
-
crude recombinant enzyme from overexpressing Yarrowia lipolytica strain Po1h
261
-
enzyme I
292
-
enzyme II
335
-
enzyme III
511
-
purified recombinant enzyme
592
-
pH 5.0, 50C
1966
-
purified native enzyme
additional information
-
44 U/mg. One unit of beta-1,3-glucanase activity is defined as the amount of the enzyme that releases reducing saccharides equivalent to 0.001 mg glucose per minute
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2
-
assay at
4.4 - 4.5
-
-
4.5 - 5
-
-
4.7 - 5.2
-
-
5.5 - 6
-
-
6.5
-
enzyme I
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.8 - 8
-
more than 50% of maximum activity within
3 - 6.5
3 - 7
-
activity range, profile, overview
4.5 - 7
-
enzyme II and III
5 - 7.5
-
enzyme I
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
activity range
30 - 55
-
activity range
30 - 80
-
30C: about 40% of maximal activity, 80C: about 65% of maximal activity
30 - 60
-
activity range, profile, overview
35 - 67
-
35C: about 70% of maximal activity, 67C: about 85% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3
-
isoelectric focusing
6
calculated from amino acid sequence
7.1
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
growth on laminarin from Laminaria digitata or beta-glucan from the fruiting bodies of Pleurotus cornucopiae
Manually annotated by BRENDA team
-
from Aspergillus saitoi
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
Streptomyces sp. (strain SirexAA-E / ActE)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
gel filtration
31500
-
gel filtration
59500
-
TSK G3000SW, gel filtration
60000
-
gel filtration
68000
-
subunit weight
70000
-
gel filtration
78000
-
SDS-PAGE and isoelectric focussing
82000
-
disc gel electrophoresis, varying polyacrylamide gel concentrations
104000
-
enzyme precursor, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
mutants F258I, E292Q, E292S /F229A and F144Y/F258Y, and mutant E292S/F229A complexed with laminaritriose
-
purified recombinant mutant E282S alone or in complex with alpha-D-glucopyranosyl fluoride and 4-nitrophenyl-beta-D-glucopyranoside, or with 4-nitrophenyl-gentiobiose, X-ray diffraction structure determination and analysis at 1.59-1.96 A resolution
-
resolution 1.85 A
-
resolution 1.9 A
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 9.5
-
recombinant enzyme, 4C, 2 h, stable
709784
3 - 8
-
-
136671
3 - 7
-
purified native enzyme, highly stable
698319
3 - 10
-
-
654998
3.1
-
44% loss of activity after 75 min, underglycosylated form 93% loss of activity after 75 min
136664
4 - 7
-
-
136658
4 - 6
-
-
136670
4.5 - 10
-
enzyme I, II and III
136681
5.5 - 10.5
-
-
136661
7
-
unstable above
136657
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
pH 3.5-7.5, stable for 24 h
30 - 40
-
purified enzyme, highly stable between for 1 h in citrate-phosphate buffer at pH 7.0
30
-
looses activity at higher temperatures
35
Fungi imperfecti
-
stable below 35C
40
-
purified recombinant enzyme, stable up to
52
-
stable at 52C for 20 min
55
-
enzyme I, stable below 55C
70
-
half-life: 21.5 min
80
-
half-life: 5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, 10 days, 21% activity loss, 4C, 10 days, 15% activity loss
-
-20C, 5 months, 25% loss of activity
-
-20C, loss of activity in some samples
-
-20C, several months, no loss of activity
-
1-4C, sodium succinate buffer pH 5.0-5.5, 3 months, no loss of activity
-
4C, 1 week, no loss of activity
-
4C, pH 5.0, stable for at least 6 months
-
crude and purified enzyme preparations, -80C, 12 months, no loss of activity
-
crude enzyme preparations, 4C, 5 weeks, 5% activity loss
-
freeze drying, 50% activity loss
-
frozen, 8 months, 50% activity loss
-
purified enzyme 4C, 4 months, no loss of activity
-
room temperature, several hours
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 mg pure protein per 10 liters culture
-
432.7fold
-
enzyme I 22fold, enzyme II 25fold, enzyme III 28fold
-
expressed in Escherichia coli
-
isoenzyme I: 47fold, isoenzyme II: 128fold
native enzyme 152fold by ammonium sulfate fractionation, ion exchange chromatography and gel filtration
-
native enzyme 28fold by ammonium sulfate fractionation, and several steps of cation exchange chromatography
-
partial purification
-
recombinant ExgP from Aspergillus oryzae culture supernatant by anion exchange and hydrophobic interaction chromatography, and gel filtration
-
recombinant His6-tagged enzyme 2.1fold from Yarrowia lipolytica strain Po1h to homogeneity
-
soluble native enzyme 115fold from strain WC91-2 to homogeneity by ultrafiltration, gel filtration, and anion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
construction of a exgA disruptant and an exgA-overexpressing mutant. The exgA-overexpressing mutant shows higher mycelial growth rate and conidial efficiency than the exgA disruptant when beta-1,3-glucan is supplied as sole carbon source. No difference in phenotype is observed between them
expression in Pichia pastoris
-
gene exg2, recombinant overexpression in Lentinula edodes strain SR-1
-
gene exgP, phylogenetic analysis, subcloning in Escherichia coli strain JM109, heterologous secretory expression in Aspergillus oryzae, the recombinant enzyme is secreted to the culture medium
-
gene GLU1 , DNa and amino acid sequence determination and analysis, expression analysis
gene WsEXG1, cloned from from both the genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, subcloning of a His6-tagged enzyme in Escherichia coli strain DH5alpha, functional overexpression in Yarrowia lipolytica strain Po1h
-
heterologous over-expression in Pichia pastoris
in Saccharomyces cervisiae
-