Information on EC 3.2.1.58 - glucan 1,3-beta-glucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.2.1.58
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,3-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
SYSTEMATIC NAME
IUBMB Comments
3-beta-D-glucan glucohydrolase
Acts on oligosaccharides, but very slowly on laminaribiose.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,3-beta-D-glucanohydrolase
-
-
-
-
AkLam33
-
-
beta-1,3-exoglucanase
-
-
beta-1,3-exoglucanase
-
-
beta-1,3-glucan exo-hydrolase
-
-
-
-
beta-1,3-glucanase
-
-
beta-1,3-glucanase
Paenibacillus ehimensis IB-X-b
-
-
-
BGN3.2
-
beta-1,3-glucanase BGN3.2 and beta-1,3-glucanase BGN3.4 are encoded by different genes
BGN3.2
Acremonium blochii C59
-
beta-1,3-glucanase BGN3.2 and beta-1,3-glucanase BGN3.4 are encoded by different genes
-
BGN3.4
-
beta-1,3-glucanase BGN3.2 and beta-1,3-glucanase BGN3.4 are encoded by different genes
BGN3.4
Acremonium blochii C59
-
beta-1,3-glucanase BGN3.2 and beta-1,3-glucanase BGN3.4 are encoded by different genes
-
Exg
P29717
-
Exg1
Komagataella pastoris X-33
-
-
-
exo (13)-beta-glucanase
-
-
-
-
exo-1,3-beta-D-glucanase
-
-
-
-
exo-1,3-beta-glucanase
-
-
-
-
Exo-1,3-beta-glucanase I/II
-
-
-
-
exo-1,3-beta-glucosidase
-
-
-
-
Exo-beta 1,3 glucanase
-
-
-
-
exo-beta-(1,3)-glucanase
-
-
exo-beta-(1,3)-glucanase
Komagataella pastoris X-33
-
-
-
exo-beta-(13)-D-glucanase
-
-
-
-
exo-beta-(13)-glucanohydrolase
-
-
-
-
exo-beta-1,3-D-glucanase
-
-
-
-
exo-beta-1,3-glucanase
-
-
-
-
exo-beta-1,3-glucanase
-
-
exo-beta-1,3-glucanase
Chaetomium thermophilum CT2
-
-
-
exo-beta-1,3-glucanase
C3VMY4
-
exo-beta-1,3-glucanase
-
-
exo-beta-1,3-glucanase
-
-
exo-beta-1,3-glucanase
A9YZW0
-
exo-beta-1,3-glucanase
-
-
exo-beta-1,3-glucanase
Wickerhamomyces anomalus K
-
-
-
exo-beta-1,3-glucanase
Wickerhamomyces anomalus NCYC 434
-
;
-
exo-beta-glucanase
-
-
Exo1
D9DBT4
-
glucan (1-->3)-beta-glucosidase
-
-
GP29
-
-
-
-
panomycocin
Wickerhamomyces anomalus NCYC 434
-
;
-
Xog1
P29717
-
additional information
-
the enzyme belongs to the glycosyl hydrolase family 5, GH5
additional information
-
the enzyme probably belongs to glycosyl hydrolase family 55, GH55
CAS REGISTRY NUMBER
COMMENTARY
9073-49-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain C59
-
-
Manually annotated by BRENDA team
Acremonium blochii C59
strain C59
-
-
Manually annotated by BRENDA team
strain RIB40
Uniprot
Manually annotated by BRENDA team
Basidiomycota QM806
QM806
-
-
Manually annotated by BRENDA team
Chaetomium sp.
-
-
-
Manually annotated by BRENDA team
Chaetomium thermophilum CT2
strain CT2
-
-
Manually annotated by BRENDA team
i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1
UniProt
Manually annotated by BRENDA team
Fungi imperfecti
-
-
-
Manually annotated by BRENDA team
Geotrichum lactis
-
-
-
Manually annotated by BRENDA team
Kluyveromyces phaseolosporus
-
-
-
Manually annotated by BRENDA team
strain X-33
-
-
Manually annotated by BRENDA team
Komagataella pastoris X-33
strain X-33
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica
strain Po1h
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica Po1h
strain Po1h
-
-
Manually annotated by BRENDA team
strain IB-X-b
-
-
Manually annotated by BRENDA team
Paenibacillus ehimensis IB-X-b
strain IB-X-b
-
-
Manually annotated by BRENDA team
isozyme PiEXO1
SwissProt
Manually annotated by BRENDA team
isozyme PiEXO2
SwissProt
Manually annotated by BRENDA team
isolated from a commercial source Kitarase-M
-
-
Manually annotated by BRENDA team
Strains X14, ts-136Me10, S288C
-
-
Manually annotated by BRENDA team
member of glycosyl hydrolase family 5
-
-
Manually annotated by BRENDA team
; mycoparasite, gene taglu
TrEMBL
Manually annotated by BRENDA team
growth in presence of cell wals from Rhizoctonia solani
-
-
Manually annotated by BRENDA team
Valerianella olitoria
-
-
-
Manually annotated by BRENDA team
Wickerhamomyces anomalus K
strain K
-
-
Manually annotated by BRENDA team
Wickerhamomyces anomalus NCYC 434
NCYC 434
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
A9YZW0, -
a cell wall degrading enzyme
physiological function
-
deletion of XOG1 leads to decrease in cellular exoglucanase activity, cell adhesion and binding of antimicrobial peptide LL-37. Antibodies against Xog1p also decrease cell adhesion. Xog1 has a role in Candida albicans adhesion to polystyrene and, by inference, attachment to host cells via direct or indirect manners
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
show the reaction diagram
-
-
-
?
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
show the reaction diagram
-
-
-
?
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
show the reaction diagram
-
-
-
-
-
4-methyl-umbelliferyl-beta-D-glucoside
4-methylumbelliferone + D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
Komagataella pastoris, Komagataella pastoris X-33
-
31% of the activity with 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
amygdalin + H2O
?
show the reaction diagram
Komagataella pastoris, Komagataella pastoris X-33
-
82.2% of the activity with laminarin
-
-
?
beta-1,3-glucan + H2O
?
show the reaction diagram
P29717
-
-
-
?
beta-1,3-glucan + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-glucose-1,3-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylpachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
cell wall of Rhizoctania solani + H2O
?
show the reaction diagram
A9YZW0, -
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
?
show the reaction diagram
-
3% of activity compared to laminaran
-
-
?
cellodextrin + H2O
D-glucose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
?
show the reaction diagram
-
-
-
-
?
CM-cellulose + H2O
D-glucose
show the reaction diagram
-
very weak hydrolysis
-
?
CM-pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
CM-pachyman + H2O
D-glucose
show the reaction diagram
-
very weak hydrolysis
-
?
curdlan + H2O
?
show the reaction diagram
-
-
-
-
?
curdlan + H2O
?
show the reaction diagram
-
-
-
-
?
curdlan + H2O
?
show the reaction diagram
-
-
-
-
?
curdlan + H2O
?
show the reaction diagram
-
-
-
-
?
curdlan + H2O
D-glucose + ?
show the reaction diagram
-, Q2Z1W1
14% of the activity with laminarin from Laminaria digitata
-
-
?
esculin + H2O
?
show the reaction diagram
Komagataella pastoris, Komagataella pastoris X-33
-
24.2% of the activity with laminarin
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
-
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
?
show the reaction diagram
-
21% of activity compared to laminaran
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
barley beta-glucan
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
barley beta-glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
barley beta-glucan
D-glucose only
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
Achlya neutral glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
Avena caryopsis glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
Acremonium persicinum extracellular glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
Claviceps glucan
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
Claviceps glucan
and laminaribiose
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
Sclerotium rolfsii glucan
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
extracted and commerical barley beta-glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
yeast glucan
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
Fungi imperfecti
-
yeast glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
yeast glucan
-
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
yeast glucan
D-glucose only
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
yeast glucan
and laminaribiose
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
Ganoderma applanatum glucan
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
oat beta-glucan
-
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
oat beta-glucan
D-glucose only
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
Basidiomycota QM806
-
Claviceps glucan, yeast glucan
and laminaribiose
?
glucan + H2O
D-glucose + gentiobiose
show the reaction diagram
Basidiomycota QM806
-
Sclerotium rolfsii glucan, Ganoderma applanatum glucan
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
-
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
almost all D-glucose
?
laminaran + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
Basidiomycota, Basidiomycota QM806
-
from Eisenia bicyclis
-
?
laminaran + H2O
?
show the reaction diagram
-
-
-
-
?
laminaran + H2O
D-glucose
show the reaction diagram
-
at pH 5.0, strictly specific for laminaran and laminarioligosaccharides, with a strict exo mode of action, with glucose being the sole reaction product even at the initial stages of hydrolysis
-
-
?
laminaribiose
6-O-glucosyl-laminaribiose
show the reaction diagram
-
transglycosylation
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
-
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
-
376% of activity compared to laminaran
-
-
?
laminariheptaose + H2O
?
show the reaction diagram
-
-
-
-
?
laminariheptaose + H2O
?
show the reaction diagram
-
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
-
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
Fungi imperfecti
-
-
-
-
?
laminarihexaose + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
-
-
-
-
?
laminarihexaose + H2O
?
show the reaction diagram
P29717
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
Q42835
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
-
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
Valerianella olitoria
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
ir
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
Fungi imperfecti
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
-
oxidized laminarin, reduced laminarin
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
Basidiomycota QM806
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
Basidiomycota QM806
-
-
different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose
show the reaction diagram
Basidiomycota QM806
-
-
-
-
?
laminarin + H2O
glycerol + glycoaldehyde + D-glucose
show the reaction diagram
-
periodate-oxidized and reduced
-
?
laminarin + H2O
glycerol + glycoaldehyde + D-glucose
show the reaction diagram
-
periodate-oxidized and reduced
-
-
-
laminarin + H2O
glycerol + glycoaldehyde + D-glucose
show the reaction diagram
Basidiomycota QM806
-
periodate-oxidized and reduced
-
?
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
Chaetomium sp.
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
A9YZW0, -
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
AkLam33 exhibits exolytic beta-1,3-hydrolytic activity releasing D-glucose, it is highly active with high activity toward smaller substrates such as laminaritetraose and laminaritriose, and hydrolyzes highly branched beta-1,3-glucans such as Eisenia bicyclis laminarin, but it is also active toward beta-1,3-glucans with a few beta-1,6-linked glucose branches such as Laminaria digitata laminarin
-
-
?
laminarin + H2O
?
show the reaction diagram
C3VMY4
exo-beta-1,3-glucanases hydrolyze laminarin by sequentially cleaving glucose residues from the non-reducing end of polymers or oligomers
-
-
?
laminarin + H2O
?
show the reaction diagram
-
high activity in strain KH10
-
-
?
laminarin + H2O
?
show the reaction diagram
-
active site glutamate residue
-
-
?
laminarin + H2O
?
show the reaction diagram
-
from Laminaria digitata or Eisenia bicyclis
-
-
?
laminarin + H2O
?
show the reaction diagram
-
from Laminarium digitatum
-
-
?
laminarin + H2O
?
show the reaction diagram
C3VMY4
the purified native enzyme can convert laminarin into monosaccharides and disaccharides, but has no killer toxin activity
-
-
?
laminarin + H2O
?
show the reaction diagram
Acremonium blochii C59
-
-
-
-
?
laminarin + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
beta-D-glucose + laminaribiose
show the reaction diagram
-
61% beta-D-glucose + 39% laminaribiose
-
-
?
laminarin + H2O
D-glucose
show the reaction diagram
Chaetomium thermophilum, Chaetomium thermophilum CT2
-
-
-
-
?
laminarin + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
D-glucose + ?
show the reaction diagram
-, Q2Z1W1
activity with laminaran from Eisnia bicyclic is 4% of the activity with laminarin from Laminaria digitata
-
-
?
laminarin + H2O
D-glucose + ?
show the reaction diagram
Paenibacillus ehimensis IB-X-b
-
-
-
-
?
laminarin + H2O
D-glucose + ?
show the reaction diagram
Komagataella pastoris X-33
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
Fungi imperfecti
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
-
different combinations and amount of these products, depending on organism
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
-
different combinations and amount of these products, depending on organism
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
-
different combinations and amount of these products, depending on organism
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
-
118% of the activity with laminaran
-
-
?
laminaripentaose + H2O
?
show the reaction diagram
P29717
-
-
-
?
laminaripentaose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaripentaose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
Fungi imperfecti
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
-
different combinations and amount of these products, depending on organism
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
-
different combinations and amount of these products, depending on organism
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
-
different combinations and amount of these products, depending on organism
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
-
121% of the activity with laminaran
-
-
?
laminaritetraose + H2O
?
show the reaction diagram
P29717
-
-
-
?
laminaritetraose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
?
show the reaction diagram
-
preferred substrate
-
-
?
laminaritetraose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
-
-
D-glucose only
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
-
115% of the activity with laminaran
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenin + H2O
D-glucose
show the reaction diagram
-
-
-
?
lichenin + H2O
D-glucose
show the reaction diagram
-
-
-
?
luteose + H2O
D-glucose
show the reaction diagram
-
-
-
?
N,N'-diacetyl-chitobiose + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-galactosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-glucosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
neutral glucan + H2O
D-glucose + laminaribiose
show the reaction diagram
-
from Achlya
-
?
nigeran + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-glucopyranoside + H2O
o-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
oligoglucosides from pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
Q42835
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside
p-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-glucoside
D-glucose + nitrophenol + glucan
show the reaction diagram
-
-
incompletely degraded
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
22% of activity compared to laminaran
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
Fungi imperfecti
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
D-glucose
show the reaction diagram
Basidiomycota QM806
-
-
-
-
?
paramylon + H2O
D-glucose
show the reaction diagram
-
-
-
?
pendulan + H2O
D-glucose
show the reaction diagram
-
-
-
?
phosphoglucan + H2O
?
show the reaction diagram
-
-
-
-
?
pullulan + H2O
?
show the reaction diagram
-
-
-
-
?
purified cell wall from Rhizoctonia solani + H2O
?
show the reaction diagram
-
25% of activity compared to laminarin
-
-
?
pustulan + H2O
?
show the reaction diagram
-
41% of activity compared to laminaran
-
-
?
pustulan + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
D-glucose
show the reaction diagram
-
-
-
?
pustulan + H2O
D-glucose
show the reaction diagram
-
-
-
?
pustulan + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
salicin + H2O
?
show the reaction diagram
-
-
-
-
?
salicin + H2O
?
show the reaction diagram
-
-
-
-
?
schizophyllan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
-
-
-
?
schizophyllan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
-
-
?
scleroglucan + H2O
?
show the reaction diagram
-
-
-
-
?
sclerotan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
glucan from Sclerotinia sclerotiorum cell wall
-
?
sclerotan + H2O
D-glucose + gentiobiose
show the reaction diagram
-
glucan from Sclerotinia sclerotiorum cell wall
-
-
?
sophorose + H2O
2 beta-D-glucose
show the reaction diagram
-
-
-
-
?
starch + H2O
?
show the reaction diagram
A9YZW0, -
-
-
-
?
maltose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme also catalyzes transglycoslation reactions
-
-
-
additional information
?
-
-
enzyme prefers small substrates, has broad specificity for beta-1,3-linkages and beta-1,6-linkages
-
-
-
additional information
?
-
-
hydrolysis with inversion of the anomeric configuration, substrate subsite structure and model of enzyme binding site
-
-
-
additional information
?
-
-
no substrate: starch, chitin, chitosan
-
-
-
additional information
?
-
-
the enzyme is activated by stress stimulus
-
-
-
additional information
?
-
Chaetomium sp.
-
the enzyme is involved in mycoparasitism, enzyme can degrade cell walls of plant pathogens including Rhizoctonia solani, Gibberella zeae, Fusarium sp., Colletotrichum gloeosporioides and Phoma sp.
-
-
-
additional information
?
-
-, Q7Z9L3
exgA expression is induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression
-
-
-
additional information
?
-
-
PAEXG1 and PAEXG2 are separately and sequentially disrupted. The resulting mutant strains show a significantly reduced efficiency of biocontrol of Botrytis cinerea. When applied to wounded apple fruit, the calculated protection level dropping from 71% (parental strain) to 8% (mutated strain). Exo-beta-1,3-glucanases play a role in the biological control of Botrytis cinerea on apple
-
-
-
additional information
?
-
A9YZW0, -
no activity with chitin, chitosan, and cellulose
-
-
-
additional information
?
-
-
no direct degradation activity with laminaribiose as a sole substrate, but the enzyme is capable of degrading it via transglycosylation reaction with laminaritriose
-
-
-
additional information
?
-
-
ExgP is active only toward glucans containing beta-1,3-linkages, such as laminarin and laminaran, no or poor activity with curdran, carboxymethyl-curdlan, lentinan, beta-glucan from Hordeum vulgare, pusturan and carboxymethylcellulose
-
-
-
additional information
?
-
-
sAkLam33 substrate specificity amd mode of action, overview. The enzyme is inactive toward laminaribiose, starch, carboxymethylcellulose, agar, beta-1,4-mannan, beta-1,4-xylan, and alginic acid, and it is specific to beta-1,3-glucosyl linkages
-
-
-
additional information
?
-
-
the enzyme can hydrolyze laminaripentaose and laminaritetraose completely in 5 min to glucose and laminaribiose or laminaritriose, whereas overnight incubation is required for complete hydrolysis of laminaritriose, substrate specificity of the purified beta-1,3-glucanase, overview
-
-
-
additional information
?
-
P29717
two surface loops, amino acids 36-47 and 101-106, might play a functional role after substrate binding. The loops could bind the enzyme to a glucan chain in the cell wall. Molecular dynamics and modelling of enzyme-substrate complexes, overview
-
-
-
additional information
?
-
-
no substrate: beta-1,4-glucans, beta-1,3/1,4-glucans or 4-nitrophenyl- and methylumbelliferyl-beta-D-glucopyranosides, carboxymethyl cellulose, avicel. Trace activity with pustulan
-
-
-
additional information
?
-
-
requirement for double-sided CH/pi interactions at the +1 subsite, and necessity for phenylalanine rather than tyrosine or tryptophan. Carbohydrate-protein interactions at the +1 subsite of Exg provide the main contribution to the transition state interaction energy
-
-
-
additional information
?
-
Wickerhamomyces anomalus K
-
PAEXG1 and PAEXG2 are separately and sequentially disrupted. The resulting mutant strains show a significantly reduced efficiency of biocontrol of Botrytis cinerea. When applied to wounded apple fruit, the calculated protection level dropping from 71% (parental strain) to 8% (mutated strain). Exo-beta-1,3-glucanases play a role in the biological control of Botrytis cinerea on apple
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-1,3-glucan + H2O
?
show the reaction diagram
P29717
-
-
-
?
cell wall of Rhizoctania solani + H2O
?
show the reaction diagram
A9YZW0, -
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
AkLam33 exhibits exolytic beta-1,3-hydrolytic activity releasing D-glucose, it is highly active with high activity toward smaller substrates such as laminaritetraose and laminaritriose, and hydrolyzes highly branched beta-1,3-glucans such as Eisenia bicyclis laminarin, but it is also active toward beta-1,3-glucans with a few beta-1,6-linked glucose branches such as Laminaria digitata laminarin
-
-
?
laminarin + H2O
?
show the reaction diagram
C3VMY4
exo-beta-1,3-glucanases hydrolyze laminarin by sequentially cleaving glucose residues from the non-reducing end of polymers or oligomers
-
-
?
laminarin + H2O
?
show the reaction diagram
-
high activity in strain KH10
-
-
?
starch + H2O
?
show the reaction diagram
A9YZW0, -
-
-
-
?
laminarin + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme is activated by stress stimulus
-
-
-
additional information
?
-
Chaetomium sp.
-
the enzyme is involved in mycoparasitism
-
-
-
additional information
?
-
-, Q7Z9L3
exgA expression is induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression
-
-
-
additional information
?
-
-
PAEXG1 and PAEXG2 are separately and sequentially disrupted. The resulting mutant strains show a significantly reduced efficiency of biocontrol of Botrytis cinerea. When applied to wounded apple fruit, the calculated protection level dropping from 71% (parental strain) to 8% (mutated strain). Exo-beta-1,3-glucanases play a role in the biological control of Botrytis cinerea on apple
-
-
-
additional information
?
-
A9YZW0, -
no activity with chitin, chitosan, and cellulose
-
-
-
additional information
?
-
-
no direct degradation activity with laminaribiose as a sole substrate, but the enzyme is capable of degrading it via transglycosylation reaction with laminaritriose
-
-
-
additional information
?
-
Wickerhamomyces anomalus K
-
PAEXG1 and PAEXG2 are separately and sequentially disrupted. The resulting mutant strains show a significantly reduced efficiency of biocontrol of Botrytis cinerea. When applied to wounded apple fruit, the calculated protection level dropping from 71% (parental strain) to 8% (mutated strain). Exo-beta-1,3-glucanases play a role in the biological control of Botrytis cinerea on apple
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AgNO3
Fungi imperfecti
-
activity enhanced by 15%
Ba2+
-
activates
Ba2+
-
activates 12% at 5 mM
Ca2+
-
enhanced activity
Ca2+
-
enhanced activity
Co2+
-
enhanced activity
Cu2+
-
10 mM, 82% residual activity
Li+
-
activates
Mg2+
-
enhanced activity
Mn2+
-
1 mM, 136% of activity
Ni2+
-
activates
Zn2+
-
enhanced activity
Mn2+
Chaetomium sp.
-
5 mM, 80% activation
additional information
-
no effect by 5 mM of Mg2+, Ca2+, Na+, K+, or Li+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
-
1,10-phenanthroline
-
40.5% inhibition at 5 mM
1-deoxynojirimycin
-
-
2-deoxy-D-glucose
-
slight
2-fluoroglucosylpyranoside
-
mechanism-based
5-Amino-1H-tetrazole
-
diazotized
Ag+
-
over 97% inhibition at 5 mM
Ammonium molybdate
-
-
Co2+
-
76.5% inhibition at 5 mM
CoSO4
Fungi imperfecti
-
-
Cu2+
-
87.7% inhibition at 5 mM
CuSO4
Fungi imperfecti
-
-
D-glucono-delta-lactone
-
-
D-glucono-delta-lactone
-
-
D-glucono-delta-lactone
-
Ki: 0.9 mM
D-glucono-delta-lactone
-
-
D-glucono-delta-lactone
-
-
D-glucono-delta-lactone
-
-
D-glucono-delta-lactone
Fungi imperfecti
-
-
D-glucono-delta-lactone
-
-
D-glucono-delta-lactone
Q42835
-
EDTA
-
1 mM, 81% of activity
EDTA
-
99% inhibition at 5 mM
EGTA
-
94.5% inhibition at 5 mM
ethanol
Fungi imperfecti
-
inhibition reversible when up to 80% ethanol concentration used
Fe2+
-
18.8% inhibition at 5 mM
-
Fe3+
Chaetomium sp.
-
pH 6.0, 5 mM, 50% activation
-
Fe3+
-
over 97% inhibition at 5 mM
-
glucono-delta-lactone
-
-
Hg2+
Fungi imperfecti
-
-
Hg2+
-
1 mM, 86% of activity
Hg2+
-
over 97% inhibition at 5 mM
HgCl2
Fungi imperfecti
-
-
indole-3-acetic acid
-
slight
iodoacetic acid
-
-
iodoacetic acid
-
-
iodoacetic acid
-
74.4% inhibition at 5 mM
KMnO4
-
10 mM, 60% residual activity
L-cysteine
-
10 mM, 68% residual activity
Laminaran
-
strong at high concentrations
Li2SO4
Fungi imperfecti
-
-
LL-37
-
antimicrobial peptide derived from human cathelicidin, by proteinase-3 cleavage. LL-37 significantly reduces Candida albicans adhesion to plastic, oral epidermoid OECM-1 cells, and urinary bladders of female BALB/c mice. LL-37 reduces Xog1 activity and thus interrupts cell-wall remodeling
-
Mn2+
-
87.5% inhibition at 5 mM
MnCl2
Fungi imperfecti
-
-
N-bromosuccinimide
-
-
N-bromosuccinimide
Fungi imperfecti
-
-
N-bromosuccinimide
-
-
N-bromosuccinimide
-
10 mM, 0.1% residual activity
Na2HAsO4
Fungi imperfecti
-
-
Ni2+
-
83.5% inhibition at 5 mM
p-chloromercuribenzoate
Fungi imperfecti
-
-
p-hydroxymercuribenzoate
-
-
periodate-oxidized and borohydride reduced laminarin
-
substrate inhibition
-
Phenyl mercuric nitrate
-
-
phenylglyoxal hydrate
-
10 mM, 86% residual activity
PMSF
-
24% inhibition at 5 mM
SDS
-
34% inhibition at 5 mM
Tetranitromethane
-
-
Zn2+
-
1 mM, 83% of activity
Zn2+
-
76% inhibition at 5 mM
ZnSO4
Fungi imperfecti
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.4
-
4-nitrophenyl beta-D-glucoside
Q42835
-
2
-
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
1.9
-
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
5
-
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
16.5
-
beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
2.1
-
beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside
-
pH 4.5, 37C
0.095
-
cellobiose
-
-
1.03
-
Laminaran
-
-
13
-
Laminaran
-
pH 5.5, 30C
0.255
-
laminaribiose
-
-
1.08
-
laminaribiose
-
30C, pH 4.5, hydrolysis
9.8
-
laminaribiose
-
pH 5.5, 30C
34.1
-
laminaribiose
-
30C, pH 4.5, transglycosylation
0.36
-
Laminarihexaose
-
-
0.0018
-
Laminarin
-
enzyme I, based on molecular mass for laminarin of 5300
0.0041
-
Laminarin
-
enzyme IIII, based on molecular mass for laminarin of 5300
0.0043
-
Laminarin
-
enzyme II, based on molecular mass for laminarin of 5300
0.066
-
Laminarin
Fungi imperfecti
-
-
0.18
-
Laminarin
-
molecular weight of 4000 assumed
0.23
-
Laminarin
-
or 1.7 mg/ml
0.85
-
Laminarin
-
molecular weight postulated to be 2448
0.0119
-
Laminaripentaose
-
30C, pH 4.5, hydrolysis
0.111
-
Laminaripentaose
-
-
0.28
-
Laminaripentaose
-
or 2.08 mg/ml
0.31
-
Laminaripentaose
-
-
2.3
-
Laminaripentaose
Fungi imperfecti
-
-
32
-
Laminaripentaose
-
30C, pH 4.5, transglycosylation
0.06
-
laminaritetraose
-
-
0.205
-
laminaritetraose
-
30C, pH 4.5, hydrolysis
0.44
-
laminaritetraose
-
-
18.6
-
laminaritetraose
Fungi imperfecti
-
-
20.9
-
laminaritetraose
-
30C, pH 4.5, trynsglycosylation
0.223
-
laminaritriose
-
30C, pH 4.5, hydrolysis
1.49
-
laminaritriose
-
-
28.3
-
laminaritriose
-
30C, pH 4.5, transglycosylation
1.9
-
methylumbelliferyl-beta-glucoside
-
mutant Q330E
0.41
-
p-nitrophenyl-beta-D-glucopyranoside
-
-
4.07
-
p-nitrophenyl-beta-D-glucopyranoside
-
under glycosylated form
6.6
-
p-nitrophenyl-beta-D-glucopyranoside
-
-
2.82
-
p-nitrophenyl-beta-D-glucoside
-
pH 5.5, 30C
5
-
p-nitrophenyl-beta-D-glucoside
-
-
0.37
-
pustulan
-
or 4.5 mg/ml
-
24.8
-
pustulan
-
pH 5.5, 30C
-
11.1
-
Gentiobiose
-
pH 5.5, 30C
additional information
-
Laminaran
-
KM-value is 1.66 mg/ml
0.83
-
Laminarihexaose
Fungi imperfecti
-
-
additional information
-
Laminarin
-
KM value of wild-type is 4.8 mg/ml, pH 5.6, 37C
2
-
methylumbelliferyl-beta-glucoside
-
-
additional information
-
additional information
-
laminarin 0.09 g/l
-
additional information
-
additional information
-
laminarin 0.008% (w/v)
-
additional information
-
additional information
-
laminarin 1.22 mg/ml; lichenin 2.22 mg/ml
-
additional information
-
additional information
-
laminarin 6.25 mg/ml; pustulan 166 mg/ml
-
additional information
-
additional information
-
laminarin 1.7 mg/ml
-
additional information
-
additional information
-
laminaran 0.080 mg/ml, laminaribiose 4.06 mg/ml , laminarihexaose 0.086 mg/ml, laminaripentaose 0.107 mg/ml, laminaritetraose 0.125 mg/ml, laminaritriose 0.172 mg/ml
-
additional information
-
additional information
-
laminarin sigmoidal kinetics, Hill coefficient 1.6, half-maximum velocity at 58 g/ml
-
additional information
-
additional information
-
laminarin 0.28 mg/ml
-
additional information
-
additional information
-
yeast cell wall 1,3-beta-glucan 2.5 mg/ml
-
additional information
-
additional information
-
laminarin 0.67 mg/ml, neutral glucan from Achlya 1.11 mg/ml, phosphoglucan 2.1 mg/ml
-
additional information
-
additional information
-
laminarin 2.5 mg/ml
-
additional information
-
additional information
-
laminarin 3.8 mg/ml, mutant E230Q 2.6 mM, mutnat E330Q 2.9 mM, mutant Q330E 2.9 mM
-
additional information
-
additional information
-
apparent Km-value for laminarin is 3.5 mg/ml
-
additional information
-
additional information
-
Michaelis-Menten kinetics, overview
-
additional information
-
additional information
-
kinetics, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
29550
-
Laminaran
-
pH 5.0, 50C
33.3
-
laminaribiose
-
30C, pH 4.5, transglycosylation
45.6
-
laminaribiose
-
30C, pH 4.5, hydrolysis
0.05
-
Laminarin
-
pH 6.0, 30C
7.7
-
Laminarin
-
mutant F144A, pH 5.6, 37C
8
-
Laminarin
-
mutant F144A/F258Y, pH 5.6, 37C
9
-
Laminarin
-
mutant F258I, pH 5.6, 37C
25
-
Laminarin
-
mutant F229A, pH 5.6, 37C
88.3
-
Laminarin
-
mutant F258W, pH 5.6, 37C
157
-
Laminarin
-
wild-type, pH 5.6, 37C
1.53
-
Laminaripentaose
-
-
33.7
-
Laminaripentaose
-
30C, pH 4.5, hydrolysis
2.3
-
laminaritetraose
-
-
4.79
-
laminaritetraose
-
pH 6.0, 30C
39
-
laminaritetraose
-
30C, pH 4.5, hydrolysis
41.8
-
laminaritriose
-
30C, pH 4.5, hydrolysis
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
Laminarin
-
kcat/KM value of wild-type is 32.7 mg per ml and sec, pH 5.6, 37C
12492
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.9
-
D-glucono-delta-lactone
-
-
1.25
-
glucono-delta-lactone
-
pH 5.0, 50C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.004
-
A9YZW0, -
growth on chitin
0.005
-
A9YZW0, -
growth on cellulose
0.006
-
A9YZW0, -
growth on chitosan
0.008
-
A9YZW0, -
growth on glucose
0.02
-
A9YZW0, -
extracellular enzyme, substrate starch; growth on starch
0.265
-
-
purified native enzyme
0.696
-
-
-
0.7
-
-
isoenzyme I
2.5
-
-
isoenzyme II
3
8
-
purified enzyme
4.7
-
Q42835
isoenzyme I
7.75
-
-
pH 5.0, 40C
11.7
-
-
purified mature enzyme
12.62
-
-
-
12.8
-
Q42835
isoenzyme II
13.77
-
-
substrate: 4-nitrophenyl-beta-D-glucopyranoside
24.2
-
-
-
62
-
-
crude enzyme from wild-type strain WC91-2
63
-
-
pH 4.5, 37C
169.4
-
Fungi imperfecti
-
-
249
-
-
crude recombinant enzyme from overexpressing Yarrowia lipolytica strain Po1h
261
-
-
enzyme I
292
-
-
enzyme II
335
-
-
enzyme III
400
-
-
-
511
-
-
purified recombinant enzyme
592
-
-
pH 5.0, 50C
1966
-
-
purified native enzyme
additional information
-
-
44 U/mg. One unit of beta-1,3-glucanase activity is defined as the amount of the enzyme that releases reducing saccharides equivalent to 0.001 mg glucose per minute
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.4
4.5
-
-
4.5
5
-
-
4.5
-
-
-
4.7
5.2
-
-
5
-
A9YZW0, -
assay at
6
-
Fungi imperfecti
-
-
6
-
Chaetomium sp.
-
-
6.5
-
-
enzyme I
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.8
8
-
more than 50% of maximum activity within
3
6.5
-
-
3
7
-
activity range, profile, overview
3
7
-
activity range
4
7
-
pH 4.0: about 85% of maximal activity, pH 7.0: about 75% of maximal activity
4.5
5.5
-
-
4.5
7
-
enzyme II and III
5
7
Chaetomium sp.
-
50% of maximal activity at pH 5.0 and pH 7.0
5
7
-
pH 5: about 40% of maximal activity, pH 7: about 25% of maximal activity
5
7.5
-
enzyme I
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
Chaetomium sp.
-
-
40
-
A9YZW0, -
assay at
50
-
-
for short incubation times, 15 min
50
-
Fungi imperfecti
-
-
55
-
-
enzyme II
60
-
-
enzyme III
65
-
-
enzyme I
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
60
-
activity range
30
55
-
activity range
30
60
-
activity range, profile, overview
30
80
-
30C: about 40% of maximal activity, 80C: about 65% of maximal activity
35
67
-
35C: about 70% of maximal activity, 67C: about 85% of maximal activity
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.3
-
-
isoelectric focusing
4.5
-
-
calculated from sequence
4.5
-
-
sequence calculation
6
-
-, Q8S313, Q8S314, Q8S315
calculated from amino acid sequence
7.1
-
-, Q8S313, Q8S314, Q8S315
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
growth on laminarin from Laminaria digitata or beta-glucan from the fruiting bodies of Pleurotus cornucopiae
Manually annotated by BRENDA team
-
expression of a fiber-specific beta-1,3-glucanase gene correlates with callose degradation and plasmodesmata opening
Manually annotated by BRENDA team
Chaetomium sp.
-
-
Manually annotated by BRENDA team
Chaetomium thermophilum CT2, Paenibacillus ehimensis IB-X-b
-
-
-
Manually annotated by BRENDA team
Valerianella olitoria
-
young
Manually annotated by BRENDA team
additional information
A9YZW0, -
the enzyme activity occurs with all carbon sources, but the highest levels are found with starch and purified cell walls from Rhizoctonia solani
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Valerianella olitoria
-
-
Manually annotated by BRENDA team
Basidiomycota QM806
-
-
-
Manually annotated by BRENDA team
Chaetomium sp.
-
-
-
Manually annotated by BRENDA team
-
the enzyme is secreted to the culture medium
-
Manually annotated by BRENDA team
Chaetomium thermophilum CT2, Komagataella pastoris X-33, Paenibacillus ehimensis IB-X-b
-
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Hordeum vulgare var. distichum
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20000
-
-
gel filtration
21000
-
-
PAGE
31500
-
-
gel filtration
40000
-
-
PAGE
43000
-
-
under glycosylated form
43000
-
-
gel filtration
43000
-
Fungi imperfecti
-
gel filtration
45600
-
-
-
59500
-
-
TSK G3000SW, gel filtration
60000
-
-
gel filtration
68000
-
-
subunit weight
70000
-
-
gel filtration
78000
-
-
SDS-PAGE and isoelectric focussing
82000
-
-
disc gel electrophoresis, varying polyacrylamide gel concentrations
104000
-
-
enzyme precursor, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 82000, SDS-PAGE
?
-
x * 85000, deglycosylated form, SDS-PAGE
?
-
x * 107000, SDS-PAGE
?
-
x * 55500, SDS-PAGE
?
Cyberlindnera jadinii, Geotrichum lactis, Kluyveromyces phaseolosporus, Saccharomyces cerevisiae
-
x * 61000, SDS-PAGE, different yeasts
?
-
x * 30000, SDS-PAGE
?
-
x * 120000, SDS-PAGE
?
-
x * 42000, SDS-PAGE
?
-
x * 81000-89000, SDS-PAGE
?
Q42835
x * 69000, isoenzyme I, SDS-PAGE; x * 71000, isoenzyme II, SDS-PAGE
?
P49426
x * 73000, SDS-PAGE
?
-
x * 66000, SDS-PAGE
?
-
x * 45000, SDS-PAGE
?
-
x * 61000, SDS-PAGE
?
-, Q8S313, Q8S314, Q8S315
x * 43200, deduced from gene sequence; x * 63500, deduced from gene sequence; x * 83000, deduced from gene sequence
?
-
x * 48600, SDS-PAGE
?
-
x * 45000, SDS-PAGE
?
Q2Z1W1
x * 78000, SDS-PAGE
?
-
x * 35000, SDS-PAGE
?
-
x * 62000
?
-
x * 83100, SDS-PAGE
?
-
x * 47500, native enzyme, SDS-PAGE
?
-
x * 33000, SDS-PAGE
?
-
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE
?
-
two fragements of 42 kDa and 47 kDa, probably generated by proteolytic cleavage
?
-
x * 40000, SDS-PAGE
?
D9DBT4
x * 74000, SDS-PAGE
dimer
-
1 * 47000 + 1 * 48000, SDS-PAGE, deglycosylated form
dimer
-
1 * 63000 + 1 * 44000, SDS-PAGE
monomer
-
1 * 49000
monomer
-
1 * 45376, calculated from sequence; 1 * 47000, SDS-PAGE
monomer
Komagataella pastoris X-33
-
1 * 45376, calculated from sequence; 1 * 47000, SDS-PAGE
-
monomer
Wickerhamomyces anomalus NCYC 434
-
1 * 49000
-
additional information
Cyberlindnera jadinii, Geotrichum lactis, Kluyveromyces phaseolosporus, Saccharomyces cerevisiae
-
exo-beta-glucanases from yeast are aggregates of many functional units
additional information
-
N-terminal sequence
additional information
-
generation of a series of enzyme-substrate complexes using molecular docking, ranging from Exg-glucose to Exg-laminarihexaose, structure optimizations followed by molecular dynamics, conducted for each complex to assess the flexibility of the substrate, of the enzyme as a whole, and of enzyme-substrate interactions, overview
additional information
-
enzyme model with active site glutamate residue, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
Komagataella pastoris X-33
-
the preproprotein is processed sequentially by signal peptidase and a Kex2-like endoprotease to yield a mature protein of 392 amino acids
-
glycoprotein
-
the processed enzyme is N-glycosylated
glycoprotein
Q2Z1W1
the recombinant enzyme is little N-glycosylated
glycoprotein
-, Q8S313, Q8S314, Q8S315
three potential N-glycosylation sites; two potential N-glycosylation sites
proteolytic modification
-, Q8S313, Q8S314, Q8S315
peroxisome-target-signal at amino acid 11; peroxisome-target-signal at amino acid 499; signal peptide cleavage site at amino acid 27
glycoprotein
-
-
glycoprotein
Wickerhamomyces anomalus NCYC 434
-
-
-
additional information
-
the native enzyme is unglycosylated
proteolytic modification
-
the preproprotein is processed sequentially by signal peptidase and a Kex2-like endoprotease to yield a mature protein of 392 amino acids
additional information
Komagataella pastoris X-33
-
the native enzyme is unglycosylated
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
mutants F258I, E292Q, E292S /F229A and F144Y/F258Y, and mutant E292S/F229A complexed with laminaritriose
-
resolution 1.85 A
-
resolution 1.9 A
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
7
-
purified native enzyme, highly stable
3
9.5
-
recombinant enzyme, 4C, 2 h, stable
3.1
-
-
44% loss of activity after 75 min, underglycosylated form 93% loss of activity after 75 min
4.5
10
-
enzyme I, II and III
7
-
-
unstable above
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
-
pH 3.5-7.5, stable for 24 h
30
40
-
purified enzyme, highly stable between for 1 h in citrate-phosphate buffer at pH 7.0
30
-
-
looses activity at higher temperatures
30
-
Chaetomium sp.
-
stable below
30
-
-
stable below 30C
35
-
Fungi imperfecti
-
stable below 35C
40
-
-
purified recombinant enzyme, stable up to
45
-
-
stable for 45 min, underglycosylated form after 45 min 35% loss of activity
45
-
-
stable below 45C
45
-
-
90 min, 85% residual activity
45
-
-
50% inactivation
50
-
-
up to 50C
50
-
-
stable below 50C
50
-
-
enzyme II and III, stable below 50C
50
-
-
stable up to, on heating for 30 min
50
-
-
90 min, 57% residual activity
50
-
-
stable at
52
-
-
stable at 52C for 20 min
55
-
-
enzyme I, stable below 55C
60
-
-
after 15 min 100% loss of activity
60
-
Chaetomium sp.
-
30 min, inactivated above
60
-
-
1 h, enzyme retains 90% of its activity
65
-
-
half-life: 55 min
65
-
-
half-life 18.3 h
70
-
-
half-life: 21.5 min
80
-
-
half-life: 5 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, loss of activity in some samples
-
crude and purified enzyme preparations, -80C, 12 months, no loss of activity
-
crude enzyme preparations, 4C, 5 weeks, 5% activity loss
-
purified enzyme 4C, 4 months, no loss of activity
-
-20C, 5 months, 25% loss of activity
-
room temperature, several hours
-
-15C, 10 days, 21% activity loss, 4C, 10 days, 15% activity loss
-
-20C, several months, no loss of activity
-
4C, 1 week, no loss of activity
-
1-4C, sodium succinate buffer pH 5.0-5.5, 3 months, no loss of activity
-
freeze drying, 50% activity loss
-
frozen, 8 months, 50% activity loss
-
4C, pH 5.0, stable for at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
enzyme I 22fold, enzyme II 25fold, enzyme III 28fold
-
native enzyme 28fold by ammonium sulfate fractionation, and several steps of cation exchange chromatography
-
2 mg pure protein per 10 liters culture
-
432.7fold
-
-
Chaetomium sp.
-
recombinant His6-tagged enzyme 2.1fold from Yarrowia lipolytica strain Po1h to homogeneity
-
soluble native enzyme 115fold from strain WC91-2 to homogeneity by ultrafiltration, gel filtration, and anion exchange chromatography
-
-
Fungi imperfecti
-
isoenzyme I: 47fold, isoenzyme II: 128fold
Q42835
22.5fold
-
recombinant ExgP from Aspergillus oryzae culture supernatant by anion exchange and hydrophobic interaction chromatography, and gel filtration
-
recombinant enzyme
Q2Z1W1
native enzyme 152fold by ammonium sulfate fractionation, ion exchange chromatography and gel filtration
-
expressed in Escherichia coli
-
partial purification
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
construction of a exgA disruptant and an exgA-overexpressing mutant. The exgA-overexpressing mutant shows higher mycelial growth rate and conidial efficiency than the exgA disruptant when beta-1,3-glucan is supplied as sole carbon source. No difference in phenotype is observed between them
-, Q7Z9L3
expression in Pichia pastoris
-
in Saccharomyces cervisiae
-
gene WsEXG1, cloned from from both the genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, subcloning of a His6-tagged enzyme in Escherichia coli strain DH5alpha, functional overexpression in Yarrowia lipolytica strain Po1h
-
gene exgP, phylogenetic analysis, subcloning in Escherichia coli strain JM109, heterologous secretory expression in Aspergillus oryzae, the recombinant enzyme is secreted to the culture medium
-
heterologous over-expression in Pichia pastoris
Q2Z1W1
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E292Q
-
inactive, crystal structure analysis
E292S/F229A
-
inactive, crystal structure analysis
F144A
-
about 1% of wild-type activity
F144A/F258A
-
inactive
F144Y/F258Y
-
about 3% of wild-type activity
F229A
-
17fold loss of hydrolytic activity
F258A
-
activity of the F258 mutants falls off steadily in the order Phe>Trp>Tyr>Ile>Ala
F258I
-
activity of the F258 mutants falls off steadily in the order Phe>Trp>Tyr>Ile>Ala
F258W
-
activity of the F258 mutants falls off steadily in the order Phe>Trp>Tyr>Ile>Ala
F258Y
-
activity of the F258 mutants falls off steadily in the order Phe>Trp>Tyr>Ile>Ala
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
the small enzyme is an ideal computational model for its family of enzymes, GH5, and can be used to create several enzyme-substrate models starting from a crystallographic glucanase inhibitor structure
agriculture
-
Paenibacillus ehimensis IB-X-b could serve as a potential wide-range biocontrol agent against pathogenic fungi
agriculture
Paenibacillus ehimensis IB-X-b
-
Paenibacillus ehimensis IB-X-b could serve as a potential wide-range biocontrol agent against pathogenic fungi
-
medicine
D9DBT4
enzyme is the major immunodominant antigen of Pythium insidiosum, causative agent of pythiosis, a life-threatening infectious disease of humans and animals in tropical and subtropical areas
food industry
-
use of enzyme as antifungal agent against green and blue mold diseases, in citrus fruits. All tested isolates of Penicillium digitatum and Penicillium italicum are susceptible to panomycocin in vitro. Effective panomycocin concentrations for 50% growth inhibition for Penicillium digitatum and Penicillium italicum are 2 and 1 microg/ml, respectively. In tests on fruit, panomycocin at concentrations equal to the concentration required for complete inhibition in vitro protects lemon fruit from decay
medicine
-
panomycocin is a natural antifungal agent against Candida infections. It is most active against Candida tropicalis, Candida pseudotropicalis and Candida glabrata with MIC90 values of 0.001 mg/ml. It displays significant activity against Candida albicans and Candida parapsilosis with MIC90 values of 0.004 and 0.002 mg/ml, respectively. For candida krisei the MIC90 value is 0.008 mg/ml
medicine
-
panomycocin is active in vitro against fungal strains that cause superficial infections. Probable use as a topical antifungal agent
medicine
Wickerhamomyces anomalus NCYC 434
-
panomycocin is active in vitro against fungal strains that cause superficial infections. Probable use as a topical antifungal agent; panomycocin is a natural antifungal agent against Candida infections. It is most active against Candida tropicalis, Candida pseudotropicalis and Candida glabrata with MIC90 values of 0.001 mg/ml. It displays significant activity against Candida albicans and Candida parapsilosis with MIC90 values of 0.004 and 0.002 mg/ml, respectively. For candida krisei the MIC90 value is 0.008 mg/ml
-