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Literature summary for 3.2.1.58 extracted from
- II. Purification and properties of the beta-1,3-glucan exo-hydrolase (1969), Biochim. Biophys. Acta, 191, 342-353.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | laminarin 0.008% (w/v) | Euglena gracilis | |
| 0.111 | - |
Laminaripentaose | - |
Euglena gracilis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 53fold | Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 5 months, 25% loss of activity | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CM-pachyman + H2O | very weak hydrolysis | Euglena gracilis | D-glucose | - |
? | |
| glucan + H2O | barley beta-glucan | Euglena gracilis | D-glucose + gentiobiose | - |
? | |
| glucan + H2O | Claviceps glucan | Euglena gracilis | D-glucose + gentiobiose | - |
? | |
| glucan + H2O | yeast glucan | Euglena gracilis | D-glucose + gentiobiose | - |
? | |
| glucan + H2O | oat beta-glucan | Euglena gracilis | D-glucose + gentiobiose | - |
? | |
| laminarin + H2O | - |
Euglena gracilis | D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose | different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose | ? | |
| laminaripentaose + H2O | - |
Euglena gracilis | D-glucose + laminaribiose + laminaritriose + laminaritetraose | different combinations and amount of these products, depending on organism | ? | |
| laminaritetraose + H2O | - |
Euglena gracilis | D-glucose + laminaribiose + laminaritriose | different combinations and amount of these products, depending on organism | ? | |
| laminaritriose + H2O | - |
Euglena gracilis | D-glucose + laminaribiose | - |
? | |
| lichenin + H2O | - |
Euglena gracilis | D-glucose | - |
? | |
| oligoglucosides from pachyman + H2O | - |
Euglena gracilis | D-glucose | - |
? | |
| pachyman + H2O | - |
Euglena gracilis | D-glucose | - |
? | |
| paramylon + H2O | - |
Euglena gracilis | D-glucose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | 5.2 | - |
Euglena gracilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | - |
Euglena gracilis |
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Release 2023.1 (January 2023)
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