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Literature summary for 3.2.1.58 extracted from
- Purification and partial characterization of an exo-beta-glucanase from the yeast Kluyveromyces aestaurii (1977), Can. J. Biochem., 55, 1001-1006.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
Laminarin | or 1.7 mg/ml | Kluyveromyces aestuarii |  |
| 0.37 | - |
pustulan | or 4.5 mg/ml | Kluyveromyces aestuarii | |
| 5 | - |
p-nitrophenyl-beta-D-glucoside | - |
Kluyveromyces aestuarii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Kluyveromyces aestuarii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces aestuarii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 8333fold | Kluyveromyces aestuarii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 250 | - |
- |
Kluyveromyces aestuarii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Kluyveromyces aestuarii | D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose | different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose | ? | |
| p-nitrophenyl-beta-D-glucoside | - |
Kluyveromyces aestuarii | D-glucose + nitrophenol + glucan | - |
? | |
| pustulan + H2O | - |
Kluyveromyces aestuarii | D-glucose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
stable at 52°C for 20 min | Kluyveromyces aestuarii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Kluyveromyces aestuarii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | - |
Kluyveromyces aestuarii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Kluyveromyces aestuarii |
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Release 2023.1 (January 2023)
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