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Literature summary for 3.2.1.58 extracted from
- Characterization of recombinant yeast exo-beta-1,3-glucanase (Exg 1p) expressed in Escherichia coli cells (2001), Biosci. Biotechnol. Biochem., 65, 1310-1314.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Laminaran | strong at high concentrations | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.82 | - |
p-nitrophenyl-beta-D-glucoside | pH 5.5, 30°C | Saccharomyces cerevisiae | |
| 9.8 | - |
laminaribiose | pH 5.5, 30°C | Saccharomyces cerevisiae | |
| 11.1 | - |
gentiobiose | pH 5.5, 30°C | Saccharomyces cerevisiae | |
| 13 | - |
Laminaran | pH 5.5, 30°C | Saccharomyces cerevisiae | |
| 24.8 | - |
pustulan | pH 5.5, 30°C | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | 3% of activity compared to laminaran | Saccharomyces cerevisiae | ? | - |
? | |
| gentiobiose + H2O | 21% of activity compared to laminaran | Saccharomyces cerevisiae | ? | - |
? | |
| laminaran + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| laminaribiose + H2O | 376% of activity compared to laminaran | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme prefers small substrates, has broad specificity for beta-1,3-linkages and beta-1,6-linkages | Saccharomyces cerevisiae | ? | - |
? | |
| p-nitrophenyl-beta-D-glucoside + H2O | 22% of activity compared to laminaran | Saccharomyces cerevisiae | p-nitrophenol + beta-D-glucose | - |
? | |
| pustulan + H2O | 41% of activity compared to laminaran | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Exg1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to, on heating for 30 min | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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