Literature summary for 3.2.1.58 extracted from

Peng, Y.; Liu, G.L.; Yu, X.J.; Wang, X.H.; Jing, L.; Chi, Z.M.
Cloning of exo-beta-1,3-glucanase gene from a marine yeast Williopsis saturnus and its overexpression in Yarrowia lipolytica (2011), Mar. Biotechnol., 13, 193-204.

Search for more literature for 3.2.1.58

Cloned(Commentary)

Cloned (Comment)	Organism
gene WsEXG1, cloned from from both the genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, subcloning of a His6-tagged enzyme in Escherichia coli strain DH5alpha, functional overexpression in Yarrowia lipolytica strain Po1h	Cyberlindnera saturnus

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	40.5% inhibition at 5 mM	Cyberlindnera saturnus
Ag+	over 97% inhibition at 5 mM	Cyberlindnera saturnus
Co2+	76.5% inhibition at 5 mM	Cyberlindnera saturnus
Cu2+	87.7% inhibition at 5 mM	Cyberlindnera saturnus
EDTA	99% inhibition at 5 mM	Cyberlindnera saturnus
EGTA	94.5% inhibition at 5 mM	Cyberlindnera saturnus
Fe2+	18.8% inhibition at 5 mM	Cyberlindnera saturnus
Fe3+	over 97% inhibition at 5 mM	Cyberlindnera saturnus
Hg2+	over 97% inhibition at 5 mM	Cyberlindnera saturnus
iodoacetic acid	74.4% inhibition at 5 mM	Cyberlindnera saturnus
Mn2+	87.5% inhibition at 5 mM	Cyberlindnera saturnus
Ni2+	83.5% inhibition at 5 mM	Cyberlindnera saturnus
PMSF	24% inhibition at 5 mM	Cyberlindnera saturnus
SDS	34% inhibition at 5 mM	Cyberlindnera saturnus
Zn2+	76% inhibition at 5 mM	Cyberlindnera saturnus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	activates 12% at 5 mM	Cyberlindnera saturnus
additional information	no effect by 5 mM of Mg2+, Ca2+, Na+, K+, or Li+	Cyberlindnera saturnus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE	Cyberlindnera saturnus
46200	-	x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE	Cyberlindnera saturnus
47500	-	x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE	Cyberlindnera saturnus

Organism

Organism	UniProt	Comment	Textmining
Cyberlindnera saturnus	C3VMY4	i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1	-
no activity in Yarrowia lipolytica	-	-	-
no activity in Yarrowia lipolytica Po1h	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme 2.1fold from Yarrowia lipolytica strain Po1h to homogeneity	Cyberlindnera saturnus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
62	-	crude enzyme from wild-type strain WC91-2	Cyberlindnera saturnus
249	-	crude recombinant enzyme from overexpressing Yarrowia lipolytica strain Po1h	Cyberlindnera saturnus
511	-	purified recombinant enzyme	Cyberlindnera saturnus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
laminarin + H2O	active site glutamate residue	Cyberlindnera saturnus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE	Cyberlindnera saturnus
More	enzyme model with active site glutamate residue, overview	Cyberlindnera saturnus

Synonyms

Synonyms	Comment	Organism
exo-beta-1,3-glucanase	-	Cyberlindnera saturnus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Cyberlindnera saturnus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	55	activity range	Cyberlindnera saturnus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	purified recombinant enzyme, stable up to	Cyberlindnera saturnus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Cyberlindnera saturnus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	9.5	recombinant enzyme, 4°C, 2 h, stable	Cyberlindnera saturnus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Cyberlindnera saturnus	sequence calculation	-	4.5

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)