Cloned (Comment) | Organism |
---|---|
gene exg2, recombinant overexpression in Lentinula edodes strain SR-1 | Lentinula edodes |
Protein Variants | Comment | Organism |
---|---|---|
additional information | downregulation of the expression of an exo-beta-1,3-glucanase, exg2, using RNA interference, phenotype, overview | Lentinula edodes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lentinan + H2O | Lentinula edodes | lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity | ? | - |
? | |
lentinan + H2O | Lentinula edodes SR-1 | lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinula edodes | - |
gene exg2 | - |
Lentinula edodes SR-1 | - |
gene exg2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Lentinula edodes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Lentinula edodes | 4-nitrophenol + N-acetyl-beta-D-glucosaminide | - |
? | |
curdlan + H2O | - |
Lentinula edodes | ? | - |
? | |
curdlan + H2O | - |
Lentinula edodes SR-1 | ? | - |
? | |
lentinan + H2O | - |
Lentinula edodes | ? | - |
? | |
lentinan + H2O | lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity | Lentinula edodes | ? | - |
? | |
lentinan + H2O | - |
Lentinula edodes SR-1 | ? | - |
? | |
lentinan + H2O | lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity | Lentinula edodes SR-1 | ? | - |
? | |
additional information | enzyme activity with different substrates in wild-type and mutant strains, overview | Lentinula edodes | ? | - |
? | |
additional information | enzyme activity with different substrates in wild-type and mutant strains, overview | Lentinula edodes SR-1 | ? | - |
? | |
pustulan + H2O | - |
Lentinula edodes | ? | - |
? | |
pustulan + H2O | - |
Lentinula edodes SR-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EXG2 | - |
Lentinula edodes |
exo-beta-1,3-glucanase | - |
Lentinula edodes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lentinula edodes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
assay at | Lentinula edodes |
General Information | Comment | Organism |
---|---|---|
malfunction | the enzyme-downregulated strain shows significantly lower lentinan degrading activity (60-70% of the wild-type strain) in the fruiting bodies 2-4 days after harvesting. The lentinan content of fresh fruiting bodies is similar in the wild-type and EXG2 downregulated strains, but in the downregulated strain, only 25.4% of the lentinan is lost after 4 days, indicating that downregulation of EXG2 enables keeping the lentinan content high longer | Lentinula edodes |