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Literature summary for 3.2.1.58 extracted from
- Purification and characterization of an exo beta-1,3-glucanase from a Fungi imperfecti (1977), Agric. Biol. Chem., 41, 493-502.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoSO4 | - |
Fungi imperfecti |  |
| CuSO4 | - |
Fungi imperfecti | |
| D-glucono-delta-lactone | - |
Fungi imperfecti | |
| ethanol | inhibition reversible when up to 80% ethanol concentration used | Fungi imperfecti | |
| Hg2+ | - |
Fungi imperfecti | |
| HgCl2 | - |
Fungi imperfecti | |
| Li2SO4 | - |
Fungi imperfecti | |
| MnCl2 | - |
Fungi imperfecti | |
| N-bromosuccinimide | - |
Fungi imperfecti | |
| Na2HAsO4 | - |
Fungi imperfecti | |
| p-chloromercuribenzoate | - |
Fungi imperfecti | |
| ZnSO4 | - |
Fungi imperfecti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
Laminarin | - |
Fungi imperfecti | |
| 0.83 | - |
Laminarihexaose | - |
Fungi imperfecti | |
| 2.3 | - |
Laminaripentaose | - |
Fungi imperfecti | |
| 18.6 | - |
laminaritetraose | - |
Fungi imperfecti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | activity enhanced by 15% | Fungi imperfecti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Fungi imperfecti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fungi imperfecti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fungi imperfecti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 169.4 | - |
- |
Fungi imperfecti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glucan + H2O | yeast glucan | Fungi imperfecti | D-glucose + gentiobiose | - |
? | |
| laminarihexaose + H2O | - |
Fungi imperfecti | D-glucose + laminarioligosaccharides | - |
? | |
| laminarin + H2O | - |
Fungi imperfecti | D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose | different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose | ? | |
| laminaripentaose + H2O | - |
Fungi imperfecti | D-glucose + laminaribiose + laminaritriose + laminaritetraose | - |
? | |
| laminaritetraose + H2O | - |
Fungi imperfecti | D-glucose + laminaribiose + laminaritriose | - |
? | |
| pachyman + H2O | - |
Fungi imperfecti | D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Fungi imperfecti |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
stable below 35°C | Fungi imperfecti |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Fungi imperfecti |
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