Cloned (Comment) | Organism |
---|---|
- |
Komagataella pastoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | apparent Km-value for laminarin is 3.5 mg/ml | Komagataella pastoris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Komagataella pastoris | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45376 | - |
1 * 45376, calculated from sequence | Komagataella pastoris |
47000 | - |
1 * 47000, SDS-PAGE | Komagataella pastoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Komagataella pastoris X-33 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the native enzyme is unglycosylated | Komagataella pastoris |
proteolytic modification | the preproprotein is processed sequentially by signal peptidase and a Kex2-like endoprotease to yield a mature protein of 392 amino acids | Komagataella pastoris |
Purification (Comment) | Organism |
---|---|
- |
Komagataella pastoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.77 | - |
substrate: 4-nitrophenyl-beta-D-glucopyranoside | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | 31% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Komagataella pastoris | 4-nitrophenol + D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 31% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Komagataella pastoris X-33 | 4-nitrophenol + D-xylose | - |
? | |
amygdalin + H2O | 82.2% of the activity with laminarin | Komagataella pastoris | ? | - |
? | |
amygdalin + H2O | 82.2% of the activity with laminarin | Komagataella pastoris X-33 | ? | - |
? | |
esculin + H2O | 24.2% of the activity with laminarin | Komagataella pastoris | ? | - |
? | |
esculin + H2O | 24.2% of the activity with laminarin | Komagataella pastoris X-33 | ? | - |
? | |
laminarin + H2O | - |
Komagataella pastoris | D-glucose + ? | - |
? | |
laminarin + H2O | - |
Komagataella pastoris X-33 | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Komagataella pastoris |
monomer | 1 * 45376, calculated from sequence | Komagataella pastoris |
Synonyms | Comment | Organism |
---|---|---|
Exg1 | - |
Komagataella pastoris |
exo-beta-(1,3)-glucanase | - |
Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Komagataella pastoris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Komagataella pastoris | calculated from sequence | - |
4.5 |