Information on EC 2.4.1.5 - dextransucrase

Word Map on EC 2.4.1.5
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.5
-
RECOMMENDED NAME
GeneOntology No.
dextransucrase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
sucrose:(1->6)-alpha-D-glucan 6-alpha-D-glucosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9032-14-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene dexYG
-
-
Manually annotated by BRENDA team
gene gtf
-
-
Manually annotated by BRENDA team
gene gtf
-
-
Manually annotated by BRENDA team
strain 121
-
-
Manually annotated by BRENDA team
strain HJ-P4, gene LcDS
UniProt
Manually annotated by BRENDA team
strain KACC 91035, isolated from dongchimi-kimchi, a watery radish kimchi
-
-
Manually annotated by BRENDA team
formerly Leuconostoc mesenteroides B-742
-
-
Manually annotated by BRENDA team
strain EG001, isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food
UniProt
Manually annotated by BRENDA team
strain EG001, isolated from lactic acid bacteria in Kimchi, a traditional Korean fermented food
UniProt
Manually annotated by BRENDA team
strain B-1299
-
-
Manually annotated by BRENDA team
strain B-512 FMC
-
-
Manually annotated by BRENDA team
Birmingham strain
-
-
Manually annotated by BRENDA team
strain CGMCC 1.544, gene dsrX
-
-
Manually annotated by BRENDA team
strain FT 045 B
-
-
Manually annotated by BRENDA team
a constitutive mutant of Leuconostoc mesenteroides FT045B
-
-
Manually annotated by BRENDA team
isolated from fermented cabbage
-
-
Manually annotated by BRENDA team
strain Lm 28
-
-
Manually annotated by BRENDA team
strain LM-0326, gene dexYG
-
-
Manually annotated by BRENDA team
strain NRRL B512-F
-
-
Manually annotated by BRENDA team
isolated from fermented cucumber
JX679020
GenBank
Manually annotated by BRENDA team
strain ATCC 10409
-
-
Manually annotated by BRENDA team
isolated from apple, gene dsrWC
UniProt
Manually annotated by BRENDA team
isolated from wheat sourdough, single copy gene
-
-
Manually annotated by BRENDA team
isolated from wheat sourdough, single copy gene
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1'-beta-D-fructofuranosyl alpha-acarbose
D-fructose + acarbose
show the reaction diagram
alpha-D-glucopyranosyl fluoride + ?
?
show the reaction diagram
luteolin + sucrose
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
show the reaction diagram
myricetin + sucrose
?
show the reaction diagram
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
show the reaction diagram
sucrose + 1,5-anhydro-D-fructose
alpha-D-glucopyranosyl-(1,6)-1,5-anhydro-D-fructose + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-O-1,5-anhydro-D-fructose + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-O-1,5-anhydro-D-fructose
show the reaction diagram
sucrose + 2-chloroethanol
2-chloroethyl alpha-D-glucopyranoside + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + 3-methyl-1-butanol
D-fructose + 3-methylbutyl alpha-D-glucoside
show the reaction diagram
sucrose + 4-chlorobutanol
4-chlorobutyl alpha-D-glucopyranoside + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + 6-chlorohexanol
6-chlorohexyl alpha-D-glucopyranoside + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + acceptor
?
show the reaction diagram
-
-
-
-
?
sucrose + alpha-butylglucopyranoside
alpha-D-glucopyranosyl-(1,6)-O-butyl-alpha-D-glucopyranoside + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-O-butyl-alpha-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
sucrose + alpha-D-glucopyranoside
?
show the reaction diagram
sucrose + butan-1-ol
butyl alpha-D-glucopyranoside + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + D-glucose
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
show the reaction diagram
sucrose + dextran
?
show the reaction diagram
sucrose + dextran
D-fructose + ?
show the reaction diagram
sucrose + dextran
D-fructose + elongated dextran
show the reaction diagram
sucrose + ethanol
D-fructose + ethyl alpha-D-glucoside
show the reaction diagram
sucrose + ethanol
ethyl alpha-D-glucopyranoside + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + gentobiose
?
show the reaction diagram
-
glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview
-
-
?
sucrose + hydroquinone
D-fructose + 4-hydroxyphenyl-alpha-D-glucopyranoside
show the reaction diagram
sucrose + isomaltohexaose
?
show the reaction diagram
sucrose + isomaltose
D-fructose + isomaltotriose
show the reaction diagram
sucrose + isomaltose
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
show the reaction diagram
sucrose + L-ascorbic acid
D-fructose + L-ascorbic acid 2-glucoside
show the reaction diagram
sucrose + lactulose
D-fructose + lactulosucrose
show the reaction diagram
sucrose + maltose
?
show the reaction diagram
-
glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview
-
-
?
sucrose + maltose
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
show the reaction diagram
sucrose + methanol
D-fructose + methyl alpha-D-glucoside
show the reaction diagram
sucrose + methanol
methyl alpha-D-glucopyranoside + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + N-(tert-butoxycarbonyl)-L-serine methyl ester
N-tert-butoxycarbonyl-3-O-alpha-D-glucopyranosyl-L-serine methyl ester + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + n-propanol
D-fructose + propyl alpha-D-glucoside
show the reaction diagram
sucrose + N-tert-butoxycarbonyl-D-serine methyl ester
N-tert-butoxycarbonyl-3-O-alpha-D-glycopyranosyl-D-serine methyl ester + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + propan-1-ol
propyl alpha-D-glucopyranoside + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + salicin
?
show the reaction diagram
-
glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview
-
-
?
sucrose + tert-butanol
D-fructose + tert-butyl alpha-D-glucoside
show the reaction diagram
-
-
-
-
?
sucrose + [(1,6)-alpha-D-glucosyl]n
D-fructose + [(1,6)-alpha-D-glucosyl]n+1
show the reaction diagram
sucrose + [(1->6)-alpha-D-glucosyl]n
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
show the reaction diagram
sucrose + dextran
D-fructose + ?
show the reaction diagram
sucrose + [(1,6)-alpha-D-glucosyl]n
D-fructose + [(1,6)-alpha-D-glucosyl]n+1
show the reaction diagram
sucrose + [(1->6)-alpha-D-glucosyl]n
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CoCl2
-
activates 22% at 4 mM
FeSO4
-
inhibits 26.5% at 0.1 mM, but activates 39.5% at 0.05 mM
Li+
activates by 15.8% at 2 mM
MgSO4
-
activates 115.6% at 0.1 mM, 20% at 0.05 mM
MnSO4
-
activates 68% at 0.1 mM, but inhibits at 0.05 mM by 26%
Na+
-
activates at 1 mM
NaCl
-
activates 11% at 0.1 mM
Ni2+
activates by 7% at 2 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1'-beta-D-fructofuranosyl alpha-acarbose
-
strong inhibition
2,4,6-trinitrobenzene-sulphonic acid
-
inactivation
2,4,6-Trinitrobenzenesulfonic acid
-
98.7% inhibition at 25 mM
2-deoxy-2-fluoro-alpha-D-glucopyranosyl fluoride
-
competitive
2-mercaptoethanol
-
120 mM, 17% inhibition
3-Deoxy-3-fluoro-alpha-D-glucopyranosyl fluoride
-
competitive
3-deoxy-3-thio-alpha-D-glucopyranosyl fluoride
-
competitive
6'-Amino-6'-deoxysucrose
-
competitive
6-Deoxy-6-fluoro-alpha-D-glucopyranoside
-
very weak, noncompetitive
6-Deoxysucrose
-
-
acarbose
-
strong inhibition
Al3+
-
strongly inhibits the native and the recombinant enzyme
alpha-D-glucopyranosyl fluoride
-
-
barium chloride
64.29% inhibition of transferase activity at 10 mM; 64.86% inhibition of transferase activity at 10 mM; 70.27% inhibition of transferase activity at 10 mM
-
beta-Glucan
-
slight inhibition
CaCl2
-
inhibits at 0.1 mM by 64%
Cd2+
23% inhibition at 2 mM
chitosan
-
various sizes of chitosans (CTSN, CTSN-P, CTSN-B, and CTSN-S) prepared as low molecular weight chitosan show significant inhibitory effect on enzyme DSase activity, antimicrobial activity of CTSNs toward Streptococcus mutans, overview
D-fructose
-
competitive
D-galactosamine
-
complete inhibition at 1% versus 1% sucrose
D-gluconic acid
-
-
D-glucono-1,5-lactone
-
competitive
D-glucosamine
-
complete inhibition at 1% versus 1% sucrose
D-glucuronic acid
-
reduced, noncompetitive
D-mannosamine
-
complete inhibition at 1% versus 1% sucrose
D-xylose
-
about 40% inhibition at 1% versus 1% sucrose
dextran derivatives
-
in which 5-50% of the D-glucose units are oxidized, acts as potent and specific inhibitor. 10%-oxidized derivatives of dextran fraction ranging in MW from 10000 Da to 2000000 Da
-
diethyldicarbonate
-
-
dithiothreitol
-
100 mM, 41% inhibition
DTNB
-
98.7% inhibition at 10 mM
FeSO4
-
inhibits 26.5% at 0.1 mM, but activates 39.5% at 0.05 mM
fructan
-
the fructan of Lactobacillus sanfranciscensis has either no effect or an inhibitory effect
-
gallic acid
-
80-90% inhibition at 4-5 mM, mixed-type inhibition in vitro
Glutaraldehyde
-
does not support enzyme stability, but inhibits the enzyme activity
Guanidine-HCl
hyaluronic acid
-
molecular weight of about 8.9 kDa
iodoacetic acid
-
98.9% inhibition at 25 mM
manganese chloride
54.05% inhibition of transferase activity at 100 mM; 59.46% inhibition of transferase activity at 100 mM; 64.29% inhibition of transferase activity at 100 mM
-
methyl 6-amino-6-deoxyglucoside
-
competitive
methyl 6-deoxy-6-fluoro-alpha-D-glucopyranoside
-
very weak, noncompetitive
methyl 6-deoxy-alpha-D-glucopyranoside
-
weak competitive
methyl alpha-D-glucopyranoside
-
-
methyl-alpha-D-glucoside
Methyl-deoxy-alpha-D-glucopyranoside
-
weak, competitive
MnSO4
-
activates 68% at 0.1 mM, but inhibits at 0.05 mM by 26%
N-Methyl-D-glucamine
-
competitive
Na+
-
slightly inhibits the native, but not the recombinant enzyme
Nojirimycin
-
noncompetitive
o-phthalaldehyde
Periodate-oxidized dextrans
-
-
-
phenylmercuric acetate
potassium chloride
30.81% inhibition of transferase activity at 100 mM; 33.33% inhibition of transferase activity at 100 mM; 35.13% inhibition of transferase activity at 100 mM
pyridoxal 5'-phosphate
-
98.5% inhibition at 25 mM, sucrose protects against the inhibition
pyridoxal-5'-phosphate
-
inactivation
Rb+
56% inhibition at 2 mM
Ribocitrin
-
-
Sn2+
34% inhibition at 2 mM
sodium lauryl sulfate
51.35% inhibition of transferase activity at 100 mM; 51.35% inhibition of transferase activity at 100 mM; 52.38% inhibition of transferase activity at 100 mM
sodium metasilicate
29.73% inhibition of transferase activity at 100 mM; 35.05% inhibition of transferase activity at 100 mM; 37.84% inhibition of transferase activity at 100 mM
Sucrose
-
the 155000 Da enzyme form is more sensitive to substrate inhibition than the 170000 Da precursor form
sulfated chitin
-
-
-
syringic acid
-
48% inhibition at 5 mM, mixed-type inhibition in vitro
Tannic acid
-
80% inhibition at 0.2 mM, mixed-type inhibition in vitro
Tris
-
competitive with sucrose
ZnSO4
-
inhibits 27% at 0.05 mM and 24.5% at 0.1 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alginate
-
80% activation
D-galactose
-
30% activation at 1%, with concentration of 1% sucrose
D-mannose
-
40% activation at 1%, with concentration of 1% sucrose
Dextrans
exopolysaccharides
-
fructan
-
activates the enzyme
-
fucoidan
-
20% activation
-
Glucan
lactose
-
50% activation at 1%, with concentration of 1% sucrose
methyl-alpha-D-glucoside
-
50 mM, activates release of D-fructose, sucrase activity, inhibits synthesis of dextran, transferase activity
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
189
1'-beta-D-fructofuranosyl alpha-acarbose
-
-
26
alpha-D-glucopyranosyl fluoride
-
-
17.8 - 416.7
Dextran
0.0086 - 492.5
Sucrose
6.2 - 13
[(1->6)-alpha-D-glucosyl]n
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.085 - 3.2
Dextran
0.4 - 1070
Sucrose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5 - 88.4
Sucrose
55
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.35
1'-beta-D-fructofuranosyl alpha-acarbose
-
-
63
2-deoxy-2-fluoro-alpha-D-glucopyranosyl fluoride
-
-
93
3-Deoxy-3-fluoro-alpha-D-glucopyranosyl fluoride
-
-
53
3-deoxy-3-thio-alpha-D-glucopyranosyl fluoride
-
-
6.6 - 18
6'-Amino-6'-deoxysucrose
1.6
6-Deoxysucrose
-
-
36
alpha-D-glucopyranosyl fluoride
-
-
6.3 - 7.5
D-gluconic acid
0.2 - 1.1
EDTA
27
fructose
-
-
1.6
gallic acid
-
pH 6.8, 37C
9.4
glucono-delta-lactone
-
sucrose concentration 12.2-97.4 mM
9.5 - 13.9
glucuronic acid
400
methyl 6-deoxy-6-fluoro-alpha-D-glucopyranoside
-
-
267
methyl 6-deoxy-alpha-D-glucopyranoside
-
-
97
methyl alpha-D-glucopyranoside
-
-
27.3
Methyl-6-amino-6-deoxyglucoside
-
sucrose concentration 1.2-4.9 mM
12
methyl-alpha-D-glucoside
-
sucrose concentration 12.2-97.4 mM
30 - 68.3
N-Methyl-D-glucamine
1.5
Nojirimycin
-
sucrose concentration 12.2-97.4 mM
1.94
syringic acid
-
pH 6.8, 37C
0.35
Tannic acid
-
pH 6.8, 37C
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.012
-
purified recombinant mutant DRN4
0.027
-
purified recombinant mutant DRN2
0.037
-
purified recombinant wild-type DSRB742
0.185
-
purified recombinant mutant DRN3
0.287
-
purified recombinant mutant DRN1
0.4
-
commercial preparation, pH 5.2, 30C
0.6
-
crude enzyme, culture supernatant, pH 5.4, 30C
1
-
partially purrified recombinant enzyme, pH 5.4, 35C
1.2
purified recombinant chimeric DSXR, dextransucrase activity, pH 5.8, 28C
1.5