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Literature summary for 2.4.1.5 extracted from

  • Ghai, K.; Batta, S.; Kocher, G.; Gupta, A.
    Production, partial purification and kinetic characterization of dextransucrase from Leuconostoc mesenteroides in relation to dextran problem in sugarcane (2015), Sugar Tech., 17, 162-172.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
barium chloride 64.29% inhibition of transferase activity at 10 mM; 64.86% inhibition of transferase activity at 10 mM; 70.27% inhibition of transferase activity at 10 mM Leuconostoc mesenteroides
EDTA 32.43% inhibition of transferase activity at 20 mM; 37.84% inhibition of transferase activity at 20 mM; 40.48% inhibition of transferase activity at 20 mM Leuconostoc mesenteroides
manganese chloride 54.05% inhibition of transferase activity at 100 mM; 59.46% inhibition of transferase activity at 100 mM; 64.29% inhibition of transferase activity at 100 mM Leuconostoc mesenteroides
additional information the compounds also inhibit the sucrase activity, overview; the compounds also inhibit the sucrase activity, overview; the compounds also inhibit the sucrase activity, overview Leuconostoc mesenteroides
potassium chloride 30.81% inhibition of transferase activity at 100 mM; 33.33% inhibition of transferase activity at 100 mM; 35.13% inhibition of transferase activity at 100 mM Leuconostoc mesenteroides
sodium lauryl sulfate 51.35% inhibition of transferase activity at 100 mM; 51.35% inhibition of transferase activity at 100 mM; 52.38% inhibition of transferase activity at 100 mM Leuconostoc mesenteroides
sodium metasilicate 29.73% inhibition of transferase activity at 100 mM; 35.05% inhibition of transferase activity at 100 mM; 37.84% inhibition of transferase activity at 100 mM Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.8
-
Dextran pH 5.5, 45°C, isozyme DS-II, low dextran substrate concentration Leuconostoc mesenteroides
18.8
-
Dextran pH 5.5, 45°C, isozyme DS-I, low dextran substrate concentration Leuconostoc mesenteroides
28.57
-
Dextran pH 5.5, 45°C, isozyme DS-III, low dextran substrate concentration Leuconostoc mesenteroides
57.14
-
Dextran pH 5.5, 45°C, isozyme DS-I, high dextran substrate concentration Leuconostoc mesenteroides
108
-
sucrose pH 5.5, 45°C, isozyme DS-III Leuconostoc mesenteroides
206.3
-
sucrose pH 5.5, 45°C, isozyme DS-I Leuconostoc mesenteroides
256.4
-
Dextran pH 5.5, 45°C, isozyme DS-II, high dextran substrate concentration Leuconostoc mesenteroides
416.7
-
Dextran pH 5.5, 45°C, isozyme DS-III, high dextran substrate concentration Leuconostoc mesenteroides
492.5
-
sucrose pH 5.5, 45°C, isozyme DS-II Leuconostoc mesenteroides

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Leuconostoc mesenteroides
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides MTCC 107
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides B2MUU6 isozyme DS-I; isozyme DS-I
-
Leuconostoc mesenteroides P85080 isozyme DS-III; isozyme DS-III
-
Leuconostoc mesenteroides P85089 isozyme DS-II; isozyme DS-II
-
Leuconostoc mesenteroides MTCC 107 B2MUU6 isozyme DS-I; isozyme DS-I
-
Leuconostoc mesenteroides MTCC 107 P85080 isozyme DS-III; isozyme DS-III
-
Leuconostoc mesenteroides MTCC 107 P85089 isozyme DS-II; isozyme DS-II
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme isozyme DS-III 8.55fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Leuconostoc mesenteroides
native extracellular isozyme DS-I 6.02fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Leuconostoc mesenteroides
native extracellular isozyme DS-II 5.95fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.83
-
30°C, purified enzyme, at start of incubation, the reaction medium has pH 7.0 Leuconostoc mesenteroides
20.83
-
30°C, purified enzyme, after 108 h of incubation, the reaction medium shows a pH of 4.0 from initially pH 7.0 Leuconostoc mesenteroides
73.31
-
30°C, purified enzyme, after 60 h of incubation, the reaction medium shows a pH of 5.5 from initially pH 7.0 Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information dextran production by dextransucrase over time at pH 7.0, 30°C, overview Leuconostoc mesenteroides ?
-
?
additional information dextran production by dextransucrase over time at pH 7.0, 30°C, overview Leuconostoc mesenteroides MTCC 107 ?
-
?
sucrose + dextran
-
Leuconostoc mesenteroides D-fructose + elongated dextran
-
?
sucrose + dextran
-
Leuconostoc mesenteroides MTCC 107 D-fructose + elongated dextran
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides MTCC 107 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Leuconostoc mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 60 purified isozyme 1, thermostable at Leuconostoc mesenteroides
10 60 purified isozyme 3, thermostable at Leuconostoc mesenteroides
10 50 purified isozyme 2, thermostable at Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Leuconostoc mesenteroides

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5 activity profile, overview Leuconostoc mesenteroides

General Information

General Information Comment Organism
additional information participation of Asp/Glu residues and His residues in both substrate binding and catalysis. The enzyme activity acidifies the reaction medium within 60 h Leuconostoc mesenteroides