Inhibitors | Comment | Organism | Structure |
---|---|---|---|
barium chloride | 64.29% inhibition of transferase activity at 10 mM; 64.86% inhibition of transferase activity at 10 mM; 70.27% inhibition of transferase activity at 10 mM | Leuconostoc mesenteroides | |
EDTA | 32.43% inhibition of transferase activity at 20 mM; 37.84% inhibition of transferase activity at 20 mM; 40.48% inhibition of transferase activity at 20 mM | Leuconostoc mesenteroides | |
manganese chloride | 54.05% inhibition of transferase activity at 100 mM; 59.46% inhibition of transferase activity at 100 mM; 64.29% inhibition of transferase activity at 100 mM | Leuconostoc mesenteroides | |
additional information | the compounds also inhibit the sucrase activity, overview; the compounds also inhibit the sucrase activity, overview; the compounds also inhibit the sucrase activity, overview | Leuconostoc mesenteroides | |
potassium chloride | 30.81% inhibition of transferase activity at 100 mM; 33.33% inhibition of transferase activity at 100 mM; 35.13% inhibition of transferase activity at 100 mM | Leuconostoc mesenteroides | |
sodium lauryl sulfate | 51.35% inhibition of transferase activity at 100 mM; 51.35% inhibition of transferase activity at 100 mM; 52.38% inhibition of transferase activity at 100 mM | Leuconostoc mesenteroides | |
sodium metasilicate | 29.73% inhibition of transferase activity at 100 mM; 35.05% inhibition of transferase activity at 100 mM; 37.84% inhibition of transferase activity at 100 mM | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.8 | - |
Dextran | pH 5.5, 45°C, isozyme DS-II, low dextran substrate concentration | Leuconostoc mesenteroides | |
18.8 | - |
Dextran | pH 5.5, 45°C, isozyme DS-I, low dextran substrate concentration | Leuconostoc mesenteroides | |
28.57 | - |
Dextran | pH 5.5, 45°C, isozyme DS-III, low dextran substrate concentration | Leuconostoc mesenteroides | |
57.14 | - |
Dextran | pH 5.5, 45°C, isozyme DS-I, high dextran substrate concentration | Leuconostoc mesenteroides | |
108 | - |
sucrose | pH 5.5, 45°C, isozyme DS-III | Leuconostoc mesenteroides | |
206.3 | - |
sucrose | pH 5.5, 45°C, isozyme DS-I | Leuconostoc mesenteroides | |
256.4 | - |
Dextran | pH 5.5, 45°C, isozyme DS-II, high dextran substrate concentration | Leuconostoc mesenteroides | |
416.7 | - |
Dextran | pH 5.5, 45°C, isozyme DS-III, high dextran substrate concentration | Leuconostoc mesenteroides | |
492.5 | - |
sucrose | pH 5.5, 45°C, isozyme DS-II | Leuconostoc mesenteroides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Leuconostoc mesenteroides | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | Leuconostoc mesenteroides | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Leuconostoc mesenteroides MTCC 107 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | B2MUU6 | isozyme DS-I; isozyme DS-I | - |
Leuconostoc mesenteroides | P85080 | isozyme DS-III; isozyme DS-III | - |
Leuconostoc mesenteroides | P85089 | isozyme DS-II; isozyme DS-II | - |
Leuconostoc mesenteroides MTCC 107 | B2MUU6 | isozyme DS-I; isozyme DS-I | - |
Leuconostoc mesenteroides MTCC 107 | P85080 | isozyme DS-III; isozyme DS-III | - |
Leuconostoc mesenteroides MTCC 107 | P85089 | isozyme DS-II; isozyme DS-II | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme isozyme DS-III 8.55fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Leuconostoc mesenteroides |
native extracellular isozyme DS-I 6.02fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Leuconostoc mesenteroides |
native extracellular isozyme DS-II 5.95fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.83 | - |
30°C, purified enzyme, at start of incubation, the reaction medium has pH 7.0 | Leuconostoc mesenteroides |
20.83 | - |
30°C, purified enzyme, after 108 h of incubation, the reaction medium shows a pH of 4.0 from initially pH 7.0 | Leuconostoc mesenteroides |
73.31 | - |
30°C, purified enzyme, after 60 h of incubation, the reaction medium shows a pH of 5.5 from initially pH 7.0 | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | dextran production by dextransucrase over time at pH 7.0, 30°C, overview | Leuconostoc mesenteroides | ? | - |
? | |
additional information | dextran production by dextransucrase over time at pH 7.0, 30°C, overview | Leuconostoc mesenteroides MTCC 107 | ? | - |
? | |
sucrose + dextran | - |
Leuconostoc mesenteroides | D-fructose + elongated dextran | - |
? | |
sucrose + dextran | - |
Leuconostoc mesenteroides MTCC 107 | D-fructose + elongated dextran | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Leuconostoc mesenteroides | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Leuconostoc mesenteroides MTCC 107 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Leuconostoc mesenteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | purified isozyme 1, thermostable at | Leuconostoc mesenteroides |
10 | 60 | purified isozyme 3, thermostable at | Leuconostoc mesenteroides |
10 | 50 | purified isozyme 2, thermostable at | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | activity profile, overview | Leuconostoc mesenteroides |
General Information | Comment | Organism |
---|---|---|
additional information | participation of Asp/Glu residues and His residues in both substrate binding and catalysis. The enzyme activity acidifies the reaction medium within 60 h | Leuconostoc mesenteroides |