Application | Comment | Organism |
---|---|---|
food industry | the dextransucrase is responsible for production of dextran with predominant alpha-(1->6) linkages that might find applications as food hydrocolloids | Weissella confusa |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Weissella cibaria |
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged DSRC39-2 in Escherichia coli strain BL21(DE3) | Weissella confusa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | rDSRC39-2 shows Michaelis-Menten-type kinetics | Weissella confusa | |
0.0086 | - |
sucrose | pH 5.4, 30°C, recombinant enzyme | Weissella confusa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Weissella cibaria | - |
- |
soluble | - |
Weissella confusa | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | Weissella cibaria | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Weissella confusa | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Weissella confusa LBAE C39-2 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Weissella cibaria LBAE K39 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella cibaria | - |
isolated from wheat sourdough, single copy gene | - |
Weissella cibaria LBAE K39 | - |
isolated from wheat sourdough, single copy gene | - |
Weissella confusa | A0A5E8F621 | isolated from wheat sourdough, single copy gene | - |
Weissella confusa LBAE C39-2 | A0A5E8F621 | isolated from wheat sourdough, single copy gene | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged DSRC39-2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatogaphy | Weissella confusa |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, about 10% activity loss after 6 days, and about 35% activity loss after 20 days | Weissella confusa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Weissella cibaria | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Weissella confusa | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Weissella confusa LBAE C39-2 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Weissella cibaria LBAE K39 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DSR | - |
Weissella cibaria |
DSR | - |
Weissella confusa |
DSRC39-2 | - |
Weissella confusa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Weissella confusa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, 30 min, stable, rapid loss of activity above 30°C | Weissella confusa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
sucrose | pH 5.4, 30°C, recombinant enzyme | Weissella confusa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Weissella confusa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Weissella confusa | sequence calculation | - |
4.6 |
General Information | Comment | Organism |
---|---|---|
evolution | dextransucrases are glucansucrases belonging to the glycoside hydrolase family GH70 | Weissella cibaria |
evolution | dextransucrases are glucansucrases belonging to the glycoside hydrolase family GH70 | Weissella confusa |
additional information | the amino acids involved in catalysis, E592, D554, D665 are conserved in the catalytic core | Weissella confusa |
physiological function | the dextransucrase is responsible for production of dextran with predominant alpha-(1->) linkages | Weissella confusa |