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Literature summary for 2.4.1.5 extracted from
- Construction of a fusion enzyme of dextransucrase and dextranase: application for one-step synthesis of isomalto-oligosaccharides (2009), Enzyme Microb. Technol., 44, 159-164.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | one-step synthesis of isomalto-oligosaccharides by a fusion enzyme of dextransucrase and dextranase | Leuconostoc mesenteroides |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type enzyme DSRBCB4 and of mutant fusion enzyme of dextransucrase and dextranase, DXSR, in Escherichia coli | Leuconostoc mesenteroides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a fusion enzyme DXSR of dextransucrase, encoded by gene dsrBCB4, and dextranase, encoded by gene dex2, for one-step synthesis of isomalto-oligosaccharides. DXSR shows 150% increased endo-dextranase activity and 98% decreased dextransucrase activity. The engineered recombinant mutant enzyme DXSR, a fusion of dextransucrase and dextranase, produces linear isomalto-oligosaccharides with DP2-DP10 using sucrose as a sole substrate. DXSR gives 30fold higher production of isomalto-oligosaccharides than that of an equal activity mixture of the two enzymes such as dextranase and dextransucrase | Leuconostoc mesenteroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 99% inhibition at 2 mM | Leuconostoc mesenteroides |  |
| Ba2+ | 10% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Cd2+ | 23% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Co2+ | 14% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Cu2+ | 65.5% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Hg2+ | complete inhibition at 2 mM | Leuconostoc mesenteroides | |
| Mg2+ | 5.7% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Mn2+ | 41% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Rb+ | 56% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Sn2+ | 34% inhibition at 2 mM | Leuconostoc mesenteroides | |
| Zn2+ | 47% inhibition at 2 mM | Leuconostoc mesenteroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | activates by 20.7% at 2 mM | Leuconostoc mesenteroides | |
| K+ | activates by 13.6% at 2 mM | Leuconostoc mesenteroides | |
| Li+ | activates by 15.8% at 2 mM | Leuconostoc mesenteroides | |
| Ni2+ | activates by 7% at 2 mM | Leuconostoc mesenteroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | - |
x * 240000, recombinant fusion protein DXSR, SDS-PAGE | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | O52224 | gene dsrBCB4 | - |
| Leuconostoc mesenteroides B-1299CB4 | O52224 | gene dsrBCB4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type DSRBCB4 and mutant fusion enzyme DXSR, partially from Escherichia coli by anion exchange chromatography and ultrafiltration | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | engineered recombinant mutant enzyme DXSR, a fusion of dextransucrase and dextranase, produces linear isomalto-oligosaccharides with DP2-DP10 using sucrose as a sole substrate. DXSR gives 30fold higher production of isomalto-oligosaccharides than that of an equal activity mixture of the two enzymes such as dextranase and dextransucrase | Leuconostoc mesenteroides | ? | - |
? | |
| additional information | engineered recombinant mutant enzyme DXSR, a fusion of dextransucrase and dextranase, produces linear isomalto-oligosaccharides with DP2-DP10 using sucrose as a sole substrate. DXSR gives 30fold higher production of isomalto-oligosaccharides than that of an equal activity mixture of the two enzymes such as dextranase and dextransucrase | Leuconostoc mesenteroides B-1299CB4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 240000, recombinant fusion protein DXSR, SDS-PAGE | Leuconostoc mesenteroides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DSRBCB4 | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
partially purified recombinant fusion enzyme DXSR, stable | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
recombinant wild-type and mutant fusion enzymes | Leuconostoc mesenteroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | partially purified recombinant wild-type DSRBCB4, stable with above 80% of maximal activity | Leuconostoc mesenteroides |
| 5 | 6.4 | partially purified recombinant fusion enzyme DXSR, stable with above 80% of maximal activity | Leuconostoc mesenteroides |
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