Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dextrans | stimulation | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Leuconostoc mesenteroides | |
EDTA | addition of Ca2+ or Co2+ restores activity; addition of dextran partially protects | Leuconostoc mesenteroides | |
Fe2+ | - |
Leuconostoc mesenteroides | |
Fe3+ | - |
Leuconostoc mesenteroides | |
Mn2+ | - |
Leuconostoc mesenteroides | |
phenylmercuric acetate | - |
Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Leuconostoc mesenteroides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Leuconostoc mesenteroides | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, slight activation | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
enzyme I and II, disc gel electrophoresis | Leuconostoc mesenteroides |
100000 | - |
enzyme III, disc gel electrophoresis | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
NRRl B-512(F) | - |
Leuconostoc mesenteroides NRRl B-512(F) | - |
NRRl B-512(F) | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 17% carbohydrate | Leuconostoc mesenteroides |
Purification (Comment) | Organism |
---|---|
multiple forms: I, II and III | Leuconostoc mesenteroides |
NRRL B-512(F) | Leuconostoc mesenteroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Leuconostoc mesenteroides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72.1 | - |
- |
Leuconostoc mesenteroides |
Storage Stability | Organism |
---|---|
4°C, pH 6.0-9.0, 4 mg/ml dextran, 24 h stable, without dextran the stable range is narrowed to pH 7.0-9.0 | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme possesses enhanced levels of sucrose hydrolyzing activity | Leuconostoc mesenteroides | ? | - |
? | |
additional information | the enzyme possesses enhanced levels of sucrose hydrolyzing activity | Leuconostoc mesenteroides NRRl B-512(F) | ? | - |
? | |
sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides NRRl B-512(F) | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Leuconostoc mesenteroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 20°C: about 60% of maximal activity, 40°C: about 50% of maximal activity | Leuconostoc mesenteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, 80% loss of activity without dextran, 30% loss of activity with addition of dextran | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | about 40% of maximal activity at pH 5.0 and pH 7.0 | Leuconostoc mesenteroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 4°C, stable at 4°C for 24 h, 4 mg/ml dextrans | Leuconostoc mesenteroides |
7 | 9 | 4°C, stable for 24 h, without dextran in solution | Leuconostoc mesenteroides |