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Literature summary for 2.4.1.5 extracted from

  • Nihira, T.; Mori, T.; Asakura, M.; Okahata, Y.
    Kinetic studies of dextransucrase enzyme reactions on a substrate- or enzyme-immobilized 27 MHz quartz crystal microbalance (2011), Langmuir, 27, 2107-2111.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information immobilisation of the enzyme directly on a 27 MHz quartz crystal microbalance, QCM, plate, or on a dextran-acceptor-QCM plate, method evaluation and binding kinetics,overview. The enzymatic activity of DSase is not affected by immobilization, possibly due to the use of a long PEG spacer group. Typical frequency changes of the dextran-immobilized QCM as a function of time in response to the addition of DSase and sucrose substrate in 50 mM acetate buffer pH 5.2, 150 mM NaCl, and 1 mM CaCl2 at 25°C Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information DSase has a high affinity for dextran as first substrate with Kd of 18 nM, kinetic analysis of 27 MHz quartz crystal microbalance, QCM, plate-immobilized enzyme, overview Leuconostoc mesenteroides
3
-
sucrose enzyme in bulk solution, pH 5.2, 25°C Leuconostoc mesenteroides
3.4
-
sucrose dextran-QCM-immobilized enzyme, pH 5.2, 25°C Leuconostoc mesenteroides
7.2
-
sucrose directly QCM-immobilized enzyme, pH 5.2, 25°C Leuconostoc mesenteroides

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enhances activity Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-

Reaction

Reaction Comment Organism Reaction ID
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1 catalytic reaction mechanism for dextran elongation, overview Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DSase can quickly proceed to elongation because the decomposition rate of the ESdex complex is very small Leuconostoc mesenteroides ?
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n DSase-catalyzed dextran elongation Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Synonyms

Synonyms Comment Organism
DSase
-
Leuconostoc mesenteroides
sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Leuconostoc mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
sucrose directly QCM-immobilized enzyme, pH 5.2, 25°C Leuconostoc mesenteroides
3.5
-
sucrose dextran-QCM-immobilized enzyme, pH 5.2, 25°C Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
assay at Leuconostoc mesenteroides