Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | citrate buffer at 0.3 M results in highest activity of wild-type and mutant strains, overview | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of diverse mutant enzymes using UV irradiation random mutagensis, mutant screeening, overview. Mutant KIBGE IB-22M20 exhibits 6.75fold increased dextransucrase activity compared to the wild-type enzyme | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
77.09 | - |
sucrose | partially purified wild-type enzyme, pH 4.5, 30°C | Leuconostoc mesenteroides | |
101.7 | - |
sucrose | partially purified mutant enzyme, pH 5.0, 35°C | Leuconostoc mesenteroides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Leuconostoc mesenteroides | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
221000 | - |
x * 221000, partially purified enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
isolated from fermented cabbage | - |
Leuconostoc mesenteroides KIBGE IB-22 | - |
isolated from fermented cabbage | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant extracellular enzymes 11.35fold and 10.27fold, respectively, by PEG 4000 fractionation | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
dextransucrae activity in different strains, i.e. KIBGE IB-06, KIBGE IB-07, KIBGE IB-08, KIBGE IB-09, KIBGE IB-10, KIBGE IB-11, KIBGE IB-12, KIBGE IB-13, KIBGE IB-19, and KIBGE IB-22, all isolated from different sources, phylogenetic tree, overview. Strain KIBGE IB-22 exhibits the highest enzyme activity | Leuconostoc mesenteroides |
31.3 | - |
partially purified wild-type enzyme, pH 4.5, 30°C | Leuconostoc mesenteroides |
173.2 | - |
partially purified mutant enzyme, pH 5.0, 35°C | Leuconostoc mesenteroides |
Subunits | Comment | Organism |
---|---|---|
? | x * 221000, partially purified enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
wild-type enzyme | Leuconostoc mesenteroides |
35 | - |
mutant enzyme | Leuconostoc mesenteroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 45 | mutant enzyme, activity range | Leuconostoc mesenteroides |
15 | 40 | wild-type enzyme, activity range | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
wild-type enzyme | Leuconostoc mesenteroides |
5 | - |
mutant enzyme | Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no enzyme activity at neutral pH by the wild-type strain | Leuconostoc mesenteroides |
4 | 7.5 | mutant enzyme, maximal activity at pH 5.0, 25% of maximal activity at pH 4.0, and 7.5% of maximal activity at pH 7.5 | Leuconostoc mesenteroides |