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Literature summary for 2.4.1.5 extracted from

  • Purama, R.K.; Goyal, A.
    Identification, effective purification and functional characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-640 (2008), Biores. Technol., 99, 3635-3642.
    View publication on PubMed
Search for more literature for 2.4.1.5

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Leuconostoc mesenteroides
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 x * 180000, SDS-PAGE Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-
Leuconostoc mesenteroides NRRL B-640
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 61fold from strain NRRL B-640 by repeated polyethylene glycol fractionation and gel filtration Leuconostoc mesenteroides
native enzyme from strain nRRL-B 640 by polyethylene glycol fractionation and gel filtration, with 23-40fold purification by 10% w/v PEG 1500 and 35-60fold of total purification, method optimization, overview Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.3
-
 purified native enzyme Leuconostoc mesenteroides

Subunits

Subunits Comment Organism
? x * 180000, SDS-PAGE Leuconostoc mesenteroides
More the purified native enzyme shows multiple molecular forms on SDS-PAGE, however the same sample shows a single band on non-denaturing native-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 assay at Leuconostoc mesenteroides