Information on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.1.1.44
-
RECOMMENDED NAME
GeneOntology No.
phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
-
-
-
-
6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
reductive carboxylation of ribulose-5-phosphate by 6PGDH is investigated using kinetic isotope effects. In the absence of 6-phosphogluconate, a slow step, which precedes the chemical process, is rate-limiting for the reaction, whereas in the presence of 6-phosphogluconate, the rate-limiting step follows the isotope-sensitive step. Full activity of 6PGDH is achieved when one subunit carries out the catalysis and the other subunit carries an unreacted 6-phosphogluconate (allosteric activator)
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
redox reaction
Q6NHC5
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
pentose phosphate pathway (oxidative branch) I
-
pentose phosphate pathway (oxidative branch) II
-
Pentose phosphate pathway
-
Glutathione metabolism
-
Metabolic pathways
-
Biosynthesis of secondary metabolites
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
6-phospho-D-gluconate:NADP+ 2-oxidoreductase (decarboxylating)
The enzyme participates in the oxidative branch of the pentose phosphate pathway, whose main purpose is to produce NADPH and pentose for biosynthetic reactions. Highly specific for NADP+. cf. EC 1.1.1.343, phosphogluconate dehydrogenase (NAD+-dependent, decarboxylating).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6-P-gluconate dehydrogenase
P80859
-
6-PGDHase
-
-
6-phospho-D-gluconate dehydrogenase
-
-
-
-
6-phospho-D-gluconate-NADP+ oxidoreductase, decarboxylating
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
Azospirillum brasilense Cd
-
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
Q6NHC5
-
6-phosphogluconate dehydrogenase
B2NIW2
-
6-phosphogluconate dehydrogenase
B2NIW4
-
6-phosphogluconate dehydrogenase
B2NIW1
-
6-phosphogluconate dehydrogenase
P00350
-
6-phosphogluconate dehydrogenase
Escherichia coli K12
P00350
-
-
6-phosphogluconate dehydrogenase
B2NIV9
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
B2NIW3
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
Q762L5
-
6-phosphogluconate dehydrogenase
Klebsiella pneumoniae NTUH-K2044
Q762L5
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
B2NIW0
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase
-
-
6-phosphogluconate dehydrogenase (decarboxylating)
-
-
-
-
6-phosphogluconate dehydrogenase Gnd1
P38720
-
6-phosphogluconate dehydrogenase, decarboxylating
-
-
6-phosphogluconate-dehydrogenase
-
-
6-phosphogluconate-dehydrogenase
-
-
-
6-phosphogluconate:NADP oxidoreductase
-
-
6-phosphogluconic carboxylase
-
-
-
-
6-phosphogluconic dehydrogenase
-
-
-
-
6-phosphonogluconate dehydrogenase
-
-
6-phosphonogluconate dehydrogenase
Q7FRX8
-
6-phosphonogluconate dehydrogenase
-
-
6-phosphonogluconate dehydrogenase
Q6WAT5
-
6PGD
-
-
6PGD
A4VX60
-
6PGDH
P00350
-
6PGDH
Escherichia coli K12
P00350
-
-
6PGDH
Q762L5
-
6PGDH
Klebsiella pneumoniae NTUH-K2044
Q762L5
-
-
6PGDH
-
-
6PGDH
-
-
6PGDH
Q7FRX8
-
6PGDH
-
-
6PGDH
Q6WAT5
-
6PGDH/Gnd1
P38720
-
D-gluconate-6-phosphate dehydrogenase
-
-
GNTZII
-
-
-
-
LlPDH
P96789
-
Os6PGDH2
-
-
phosphogluconic acid dehydrogenase
-
-
-
-
YpjI
P80859
-
CAS REGISTRY NUMBER
COMMENTARY
9073-95-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
sycamore cells
-
-
Manually annotated by BRENDA team
Columbia, strain T87
-
-
Manually annotated by BRENDA team
Arthrobacter globiformis VKM B-175
VKM B-175
-
-
Manually annotated by BRENDA team
wild oat
-
-
Manually annotated by BRENDA team
gene yqjI, enzyme YpjI
SwissProt
Manually annotated by BRENDA team
silver beet
-
-
Manually annotated by BRENDA team
No. 2247 ATCC 14067
-
-
Manually annotated by BRENDA team
ATCC 32195; methanol utilizing yeast
-
-
Manually annotated by BRENDA team
methanol utilizing yeast
-
-
Manually annotated by BRENDA team
crucian carp
-
-
Manually annotated by BRENDA team
strain B/S
-
-
Manually annotated by BRENDA team
Catharanthus roseus B/S
strain B/S
-
-
Manually annotated by BRENDA team
hazel nut
-
-
Manually annotated by BRENDA team
Corynebacterium glutamicum ssp. flavum
-
-
-
Manually annotated by BRENDA team
fragment. Its is proposed that plastid-lacking excavata acquire cyanobacterial gnd genes via eukaryote-to-eukaryote lateral gene transfer or primary endosymbiotic gene transfer early in eukaryotic evolution, and then lose either their pre-existing or cyanobacterial gene
SwissProt
Manually annotated by BRENDA team
fragment. Its is proposed that plastid-lacking excavata acquire cyanobacterial gnd genes via eukaryote-to-eukaryote lateral gene transfer or primary endosymbiotic gene transfer early in eukaryotic evolution, and then lose either their pre-existing or cyanobacterial gene
SwissProt
Manually annotated by BRENDA team
type I, type II
-
-
Manually annotated by BRENDA team
cellular slime mould, strain NC-4, haploid
-
-
Manually annotated by BRENDA team
fragment. Its is proposed that plastid-lacking excavata acquire cyanobacterial gnd genes via eukaryote-to-eukaryote lateral gene transfer or primary endosymbiotic gene transfer early in eukaryotic evolution, and then lose either their pre-existing or cyanobacterial gene
SwissProt
Manually annotated by BRENDA team
ATCC 27792, formerly designated as strain MR
-
-
Manually annotated by BRENDA team
JM109, M15
-
-
Manually annotated by BRENDA team
MRE 600
-
-
Manually annotated by BRENDA team
RW226/pLC33-5
-
-
Manually annotated by BRENDA team
strain K12
UniProt
Manually annotated by BRENDA team
Escherichia coli JM109 M15
JM109, M15
-
-
Manually annotated by BRENDA team
Escherichia coli K12
strain K12
UniProt
Manually annotated by BRENDA team
Escherichia coli MRE 600
MRE 600
-
-
Manually annotated by BRENDA team
Escherichia coli RW226/pLC33-5
RW226/pLC33-5
-
-
Manually annotated by BRENDA team
fragment. Its is proposed that plastid-lacking excavata acquire cyanobacterial gnd genes via eukaryote-to-eukaryote lateral gene transfer or primary endosymbiotic gene transfer early in eukaryotic evolution, and then lose either their pre-existing or cyanobacterial gene
SWissProt
Manually annotated by BRENDA team
chicken
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus NCA 1503
NCA 1503
-
-
Manually annotated by BRENDA team
fragment. Its is proposed that plastid-lacking excavata acquire cyanobacterial gnd genes via eukaryote-to-eukaryote lateral gene transfer or primary endosymbiotic gene transfer early in eukaryotic evolution, and then lose either their pre-existing or cyanobacterial gene
SwissProt
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
human; Krebs ascites cells
-
-
Manually annotated by BRENDA team
strain NTUH-K2044
UniProt
Manually annotated by BRENDA team
Klebsiella pneumoniae NTUH-K2044
strain NTUH-K2044
UniProt
Manually annotated by BRENDA team
mouse
-
-
Manually annotated by BRENDA team
cv. Samsun, healthy plants and plants infected with potato virus Y
-
-
Manually annotated by BRENDA team
Nicotiana tabacum W-38
W-38
-
-
Manually annotated by BRENDA team
rabbit, Primiparous New Zealand white
-
-
Manually annotated by BRENDA team
cytosolic isozyme
Q7FRX8
SwissProt
Manually annotated by BRENDA team
lamb; sheep
-
-
Manually annotated by BRENDA team
fragment. Its is proposed that plastid-lacking excavata acquire cyanobacterial gnd genes via eukaryote-to-eukaryote lateral gene transfer or primary endosymbiotic gene transfer early in eukaryotic evolution, and then lose either their pre-existing or cyanobacterial gene
SwissProt
Manually annotated by BRENDA team
filamentous cyanobacterium
-
-
Manually annotated by BRENDA team
L. cv. Lincoln
-
-
Manually annotated by BRENDA team
P. fluorescens
-
-
Manually annotated by BRENDA team
Charles River albino; rat
-
-
Manually annotated by BRENDA team
male and femal Wistar rats, age 1-20 months
-
-
Manually annotated by BRENDA team
rat; Sprague-Dawley
-
-
Manually annotated by BRENDA team
rat; Wistar strain
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar strain
-
-
Manually annotated by BRENDA team
castor bean, Baker 296 'Dwarf'
-
-
Manually annotated by BRENDA team
baker's yeast
-
-
Manually annotated by BRENDA team
fission yeast, NCYC 132S2-2
-
-
Manually annotated by BRENDA team
spinach, var. Long Standing Bloomsdale
-
-
Manually annotated by BRENDA team
serotype 2
SwissProt
Manually annotated by BRENDA team
serotype 2
-
-
Manually annotated by BRENDA team
mesophilic cyanobacterium, formerly called Coccochloris peniocystis; thermophilic cyanobacterium, formerly called Synechococcus lividus
-
-
Manually annotated by BRENDA team
Synechococcus sp. PCC 6307
-
-
-
Manually annotated by BRENDA team
Synechococcus sp. PCC 6307
mesophilic cyanobacterium, formerly called Coccochloris peniocystis
-
-
Manually annotated by BRENDA team
thermophilic cyanobacterium, formerly called Synechococcus lividus
-
-
Manually annotated by BRENDA team
heterotrophic strain, formerly called Aphanocapsa sp.
-
-
Manually annotated by BRENDA team
parasitic protozoan
Uniprot
Manually annotated by BRENDA team
CL Brener clone
SwissProt
Manually annotated by BRENDA team
black gram, mung bean
-
-
Manually annotated by BRENDA team
black gram, mung bean
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
Q6NHC5
pentose phosphate pathway
metabolism
-
phosphogluconate pathway, bacterial 6-phosphogluconate dehydrogenase is a constitutive enzyme, bacterial enzyme is active in young, non-inoculated mesquite seedlings growing under hydroponic conditions, external gluconate has no effect on the activity of 6-phosphogluconate dehydrogenase
metabolism
Azospirillum brasilense Cd
-
phosphogluconate pathway, bacterial 6-phosphogluconate dehydrogenase is a constitutive enzyme, bacterial enzyme is active in young, non-inoculated mesquite seedlings growing under hydroponic conditions, external gluconate has no effect on the activity of 6-phosphogluconate dehydrogenase
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-3-oxo 6-phosphogluconate + ?
?
show the reaction diagram
-
-
-
-
?
2-deoxy-3-oxo 6-phosphogluconate + ?
?
show the reaction diagram
-
no decarboxylation by the liver enzyme
-
-
-
2-deoxy-6-phospho-D-gluconate + NADP+
3-oxo-2-deoxy-6-phosphogluconate + NADPH
show the reaction diagram
-
-
-
-
?
2-deoxy-6-phosphogluconate + NADP+
3-oxo-2-deoxy-6-phosphogluconate + NADPH
show the reaction diagram
-
-
-
-
r
2-deoxy-6-phosphogluconate + NADP+
3-oxo-2-deoxy-6-phosphogluconate + NADPH
show the reaction diagram
-
oxidation, 1.5% activity of that of 6-phosphogluconate
-
-
r
2-deoxy-6-phosphogluconate + NADP+
2-deoxy-3-oxo 6-phosphogluconate + NADPH
show the reaction diagram
-
-
-
-
r
2-deoxy-6-phosphogluconate + NADP+
2-deoxy-3-oxo 6-phosphogluconate + NADPH
show the reaction diagram
-
-
-
-
-
2-deoxy-6-phosphogluconate + NADP+
2-deoxy-3-oxo 6-phosphogluconate + NADPH
show the reaction diagram
-
-
-
r
2-deoxy-6-phosphogluconate + NADP+
2-deoxy-3-oxo 6-phosphogluconate + NADPH
show the reaction diagram
-
-
-
r
3-keto-6-phosphono-D-gluconate + ?
D-ribulose 5-phosphate + CO2
show the reaction diagram
P00349
via 1,2-enediol intermediate
-
-
-
3-keto-6-phosphono-D-gluconate + ?
D-ribulose 5-phosphate + CO2
show the reaction diagram
-
via 1,2-enediol intermediate
-
-
-
3-keto-6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2
show the reaction diagram
-
via 1,2-enediol intermediate
-
-
?
3-keto-6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2
show the reaction diagram
P31072
via 1,2-enediol intermediate
-
-
?
3-keto-6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2
show the reaction diagram
-
via 1,2-enediol intermediate
-
-
?
3-oxo-2-deoxy-6-phosphogluconate + NADP+
1-deoxyribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
reaction requires presence of NADPH, but the reduced coenzyme does not play a redox role
-
-
-
3-oxo-2-deoxy-6-phosphogluconate + NADP+
1-deoxyribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
decarboxylation
-
-
r
3-oxo-2-deoxy-6-phosphogluconate + NADPH
2-deoxy-6-phosphogluconate + NADP+
show the reaction diagram
-
-
-
-
r
3-oxo-2-deoxy-6-phosphogluconate + NADPH
2-deoxy-6-phosphogluconate + NADP+
show the reaction diagram
-
reduction
-
-
r
6,7-dideoxy-7-phosphono-D-glucoheptonate + NADP+
?
show the reaction diagram
P00349
-
-
-
?
6,7-dideoxy-7-phosphono-D-glucoheptonate + NADP+
?
show the reaction diagram
P31072
-
-
-
?
6-phospho-D-gluconate + NAD+
D-ribulose 5-phosphate + CO2 + NADH
show the reaction diagram
-
-
-
-
-
6-phospho-D-gluconate + NAD+
D-ribulose 5-phosphate + CO2 + NADH
show the reaction diagram
-
the enzyme prefers NADP+ to NAD+ as an electron acceptor
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
P31072
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
[Brevibacterium] flavum
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
ir
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Corynebacterium glutamicum ssp. flavum
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
P38720
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
P00350
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Q762L5
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
endosperm contains both cytosolic and plastic glycolytic and pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
[Brevibacterium] flavum
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
mammary gland enzyme involved in NADPH production which is essential for lipogenesis
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
pivotal enzyme links the gluconate route and the oxidative phase of the pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
oxidative enzyme of pentose phosphate pathway
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
the enzyme prefers NADP+ to NAD+ as an electron acceptor
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Escherichia coli JM109 M15
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Nicotiana tabacum W-38
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Nicotiana tabacum W-38
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Azospirillum brasilense Cd
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Escherichia coli RW226/pLC33-5
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Synechococcus sp. PCC 6307
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Synechococcus sp. PCC 6307
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Escherichia coli MRE 600
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Catharanthus roseus B/S
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Rattus norvegicus Wistar
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Geobacillus stearothermophilus NCA 1503
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Geobacillus stearothermophilus NCA 1503
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Geobacillus stearothermophilus NCA 1503
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-, enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Escherichia coli K12
P00350
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Klebsiella pneumoniae NTUH-K2044
Q762L5
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Arthrobacter globiformis VKM B-175
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
Q6NHC5
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
Azospirillum brasilense Cd
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + NADPH + CO2 + H+
show the reaction diagram
-
-
-
-
ir
6-phosphomannoate + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphomannoate + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Q7FRX8
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Q6WAT5
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-, P80859
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Q7FRX8
one of the key enzymes in the pentose phosphate pathway, may be important for cell division and salt response
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-, P80859
part of oxidative pentosephosphate pathway, predominant enzyme in glucose and cluconate catabolism, pathway regulation, overview
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
the enzyme expression and activity is independently regulated in different tissues
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
the glucose-6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase ratio in plants infected with potato virus Y is lower than in healthy plants, with glucose 6-phosphate dehydrogenase being the regulating enzyme of the oxidative pentosephosphate pathway
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
third step in pentose phosphate metabolic pathway
-
-
?
6-phosphono-D-gluconate + NADP+
3-keto-6-phosphono-D-gluconate + NADPH
show the reaction diagram
P00349
-
-
-
-
6-phosphono-D-gluconate + NADP+
3-keto-6-phosphono-D-gluconate + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphono-D-gluconate + NADP+
3-keto-6-phosphono-D-gluconate + NADPH
show the reaction diagram
-
-
-
-
-
6-phosphono-D-gluconate + NADP+
3-keto-6-phosphono-D-gluconate + NADPH
show the reaction diagram
P31072
-
-
-
?
6-phosphono-D-gluconate + NADP+
3-keto-6-phosphono-D-gluconate + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + NADPH
show the reaction diagram
P00349
overall reaction
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + NADPH
show the reaction diagram
-
overall reaction
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + NADPH
show the reaction diagram
P31072
overall reaction
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + NADPH
show the reaction diagram
-
overall reaction
-
-
?
beta-3-acetylpyridine adenine dinucleotide 2'-phosphate + 6-phosphogluconate
?
show the reaction diagram
-
-
-
-
?
beta-nicotinamide 1-N6-ethenoadenine dinucleotide 2'-phosphate + 6-phosphogluconate
?
show the reaction diagram
-
-
-
-
?
beta-nicotinamide adenine dinucleotide 3'-phosphate + 6-phosphogluconate
?
show the reaction diagram
-
-
-
-
?
beta-nicotinamide hypoxanthine dinucleotide 2'-phosphate + 6-phosphogluconate
?
show the reaction diagram
-
-
-
-
?
D-ribulose 5-phosphate + CO2 + NADPH
6-phospho-D-gluconate + NADP+
show the reaction diagram
-
-
-
-
r
ribulose 5-phosphate + H2O + NADPH
?
show the reaction diagram
-
-
-
-
?
ribulose 5-phosphate + H2O + NADPH
?
show the reaction diagram
-
tritium exchange reaction between tritiated ribulose 5-phosphate and water, reaction requires presence of NADPH, but the reduced coenzyme does not play a redox role
-
-
?
D-ribulose 5-phosphate + CO2 + NADPH
6-phospho-D-gluconate + NADP+
show the reaction diagram
-
reverse reaction only to a very limited extent
-
-
r
additional information
?
-
-
-
-
-
-
additional information
?
-
-
6-phosphogalactonate is no substrate
-
-
-
additional information
?
-
-
neither gluconic acid nor glucose-6-phosphate can replace 6-phosphogluconate as substrate
-
-
-
additional information
?
-
-
glucose-6-phosphate is no substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
P31072
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
ir
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Corynebacterium glutamicum ssp. flavum
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
endosperm contains both cytosolic and plastic glycolytic and pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
[Brevibacterium] flavum
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
mammary gland enzyme involved in NADPH production which is essential for lipogenesis
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
pivotal enzyme links the gluconate route and the oxidative phase of the pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
oxidative enzyme of pentose phosphate pathway
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
Q6NHC5
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Escherichia coli JM109 M15
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Nicotiana tabacum W-38
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH + H+
show the reaction diagram
Azospirillum brasilense Cd
-
-
-
-
?
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Escherichia coli RW226/pLC33-5
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Synechococcus sp. PCC 6307
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Escherichia coli MRE 600
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Catharanthus roseus B/S
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Rattus norvegicus Wistar
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Geobacillus stearothermophilus NCA 1503
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Geobacillus stearothermophilus NCA 1503
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Geobacillus stearothermophilus NCA 1503
-
-
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
enzyme specific for the oxidative pentose phosphate pathway
-
-
r
6-phospho-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Arthrobacter globiformis VKM B-175
-
-
-
-
r
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
Q7FRX8
one of the key enzymes in the pentose phosphate pathway, may be important for cell division and salt response
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-, P80859
part of oxidative pentosephosphate pathway, predominant enzyme in glucose and cluconate catabolism, pathway regulation, overview
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
the enzyme expression and activity is independently regulated in different tissues
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
the glucose-6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase ratio in plants infected with potato virus Y is lower than in healthy plants, with glucose 6-phosphate dehydrogenase being the regulating enzyme of the oxidative pentosephosphate pathway
-
-
?
6-phosphono-D-gluconate + NADP+
D-ribulose 5-phosphate + CO2 + NADPH
show the reaction diagram
-
third step in pentose phosphate metabolic pathway
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3-acetylpyridine adenine dinucleotide phosphate
-
APADP
3-acetylpyridine-NADP+
-
-
deamino-NADP+
-
-
NAD+
-
reacts also with NADP+
NAD+
-
some activity with NAD+
NAD+
-
NAD+ specific
NAD+
-
uses NAD+ very poorly as a substrate
NAD+
-
the enzyme prefers NADP+ to NAD+ as an electron acceptor
NADP+
-
no reaction with NAD+
NADP+
-
reacts also with NAD+
NADP+
-
does not utilize NAD+
NADP+
-
NAD+ is completely ineffective as hydrogen acceptor
NADP+
-
specific for NADP+, no activity observed when NAD+ is substituted for NADP+
NADP+
-
strictly specific for NADP+, no activity observed with NAD+
NADP+
-
specific for NADP+, unable to utilize NAD+
NADP+
-
NAD+ being totally inactive
NADP+
-
no activity observed when NAD+ is substituted for NADP+
NADP+
-
specific to NADP+
NADP+
-
absolutely specific with respect to NADP+, no reaction can be detected in the presence of NAD+
NADP+
-
uses NAD+ very poorly as a substrate
NADP+
-
requires NADP+ obligatorily for activity
NADP+
-, P80859
dependent on
NADP+
Q7FRX8
-
NADP+
-
the enzyme prefers NADP+ to NAD+ as an electron acceptor
nicotinamide 1,N6-ethenoadenine dinucleotide phosphate
-
epsilon-NADP+
nicotinamide adenine dinucleotide 3'-phosphate
-
3'-NADP+
nicotinamide hypoxanthine dinucleotide phosphate
-
HX-NADP+
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activated by 1 mM CaCl2, relative activity 146.6%
Co2+
-
activated by divalent metal ions
KCl
-
activates the enzyme at high concentrations
Mg2+
-
activates the enzyme at low concentrations 0.01-0.04 M
Mg2+
-
-
Mg2+
-
MgCl2 stimulation for the oxidative decarboxylation of 6-phosphogluconate
Mg2+
-
requires Mg2+ for full activity
Mg2+
-
activated by MgCl2
MgCl2
-
10 mM are included in assay medium
Mn2+
-
activated by 1 mM MnCl2, relative activity 142.1%
Mn2+
-
activated by divalent metal ions
NaCl
-
activates the enzyme at high concentrations
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(-)-catechin gallate
-
NADP+-competitive inhibitor of glucose-6-phosphate dehydrogenase
(-)-epicatechin gallate
-
-
(-)-epigallocatechin
-
NADP+-competitive inhibitor of glucose-6-phosphate dehydrogenase
(-)-epigallocatechin gallate
-
NADP+-competitive inhibitor of glucose-6-phosphate dehydrogenase
(-)-gallocatechin gallate
-
NADP+-competitive inhibitor of glucose-6-phosphate dehydrogenase
(2E)-2-cyano-3-(5-nitrofuran-2-yl)prop-2-enoic acid
-
-
(2R)-(2-hydroxy-3-phosphonooxypropane-1-sulfinyl)acetic acid
-
compound shows in vitro antiparasitic activity
(2R)-(2-hydroxy-3-phosphonooxypropane-1-sulfinyl)acetic acid
-
-
(2R)-(2-hydroxy-3-phosphonooxypropane-1-sulfinyl)acetic acid
-
compound shows in vitro antiparasitic activity
(2R)-[3-(bis(benzyloxy)phosphoryloxy)-2-(tetrahydropyran-2-yloxy)propane-1-sulfinyl]acetic acid
-
compound shows in vitro antiparasitic activity
(2R,3R)-2,3,4,N-tetrahydroxybutyramide
-
compound shows in vitro antiparasitic activity
(3aR,6aR)-2,2-dimethyldihydrofuro[3,4-d][1,3]dioxol-4-one
-
compound shows in vitro antiparasitic activity
(3R,4R)-3,4-di(tetrahydro-2H-pyranyloxy)tetrahydro-2-furanone
-
compound shows in vitro antiparasitic activity
(4R,5R)-5-hydroxymethyl-2,2-dimethyl-[1,3]dioxolane-4-carboxylic acid benzyloxyamide
-
compound shows in vitro antiparasitic activity
(4S)-trans-4-ethylammonio-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
i.e. dorzolamide, competitive
(5-(dimethylamine)naphthalin-1-sulfonylchloride)
-
dansyl chloride, DNSCl, rapid inactivation at pH 7.5, 95% inhibition at pH 6.2
1,5-diphospho-D-ribulose
-
-
1,5-diphospho-D-ribulose
P31072
-
1,5-diphospho-ribitol
-
-
1,5-diphospho-ribitol
P31072
-
2-deoxy-6-phosphono-D-gluconate
P31072
-
2-deoxy-6-phosphono-D-gluconate
-
compound shows in vitro antiparasitic activity
2-deoxy-6-phosphono-D-gluconate
-
-
2-deoxy-6-phosphono-D-gluconate
-
compound shows in vitro antiparasitic activity
2-methyl-5-nitrofuran-3-carboxylic acid
-
-
3,3',4,4'-tetramethyl-1H,1'H-2,2'-bipyrrole-5,5'-dicarboxylic acid
-
-
3-methyl-4-oxo-4,7-dihydro-5H-thiopyrano[3,4-b]furan-2-carboxylic acid
-
-
3-Oxo-2-deoxy-6-phosphogluconate
-
competitive inhibitor
4,5-dichloro-1H-pyrrole-2-carboxylic acid
-
-
4,N-dihydroxy(2R,3R)-2,3-bis(tetrahydropyran-2-yloxy)butyramide
-
compound shows in vitro antiparasitic activity
4-chloro-1H-pyrrole-2-carboxylic acid
-
-
4-phospho-D-erythronamide
-
compound shows in vitro antiparasitic activity
4-phospho-D-erythronamide
-
-
4-phospho-D-erythronamide
-
compound shows in vitro antiparasitic activity
4-phospho-D-erythronate
-
bio-isosteric analogue
4-phospho-D-erythronate
P31072
bio-isosteric analogue
4-phospho-D-erythronate
-
compound shows in vitro antiparasitic activity
4-phospho-D-erythronate
-
-
4-phospho-D-erythronate
-
compound shows in vitro antiparasitic activity
4-phospho-D-erythronate
-
mimics the transition state of dehydrogenation. The inhibitor forces the catalytic lysine 185 into the protonated state
4-phospho-D-erythronohydroxamic acid
-
compound shows low in vitro antiparasitic activity
4-phospho-D-erythronohydroxamic acid
-
-
4-phospho-D-erythronohydroxamic acid
-
compound shows low in vitro antiparasitic activity
4-phospho-D-erythronohydroxamic acid
-
conversion of the 6-PGDH inhibitor into prodrugs to increase its activity against the Trypanosoma brucei by enhancing uptake by passive permeation across the plasma membrane. Production of five different phosphate-masking groups (phosphoramidate, bis-S-acyl thioethyl esters, bis-pivaloxymethyl, cycloSaligenyl and phenyl, S-acyl thioethyl mixed phosphate esters). Prodrugs are studied for stability and activity against the intact parasites. Most prodrugs cause inhibition of the growth of the parasites. The activity of the prodrugs against the parasites appears to be related to their stability in aqueous buffer
4-phospho-D-erythronohydroxamic acid
-, P96789
-
4-phospho-D-erythrose
-
bio-isosteric analogue
4-phospho-D-erythrose
P31072
bio-isosteric analogue
4H-thieno[3,2-b]pyrrole-5-carboxylic acid
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
DTNB
5,5'-dithiobis(2-nitrobenzoic acid)
-
Ellman's reagent
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5,6-dideoxy-6-phosphono-D-arabino-hexonate
-
-
5,6-dideoxy-6-phosphono-D-arabino-hexonate
P31072
-
5-(3,7,12-trioxo-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
weak inhibition
5-(3alpha,7alpha,12alpha-triacetoxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
competitive
5-(3alpha,7alpha,12alpha-trihydroxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
competitive
5-(ethoxycarbonyl)-3,4-dimethyl-1H-pyrrole-2-carboxylic acid
-
-
5-deoxy-5-phosphonoD-arabinonate
-
-
5-deoxy-5-phosphonoD-arabinonate
P31072
-
5-phospho-D-arabinonamide
-
-
5-phospho-D-arabinonamide
P31072
-
5-phospho-D-arabinonate
-
-
5-phospho-D-arabinonate
P31072
-
5-phospho-D-arabinonhydrazide
-
-
5-phospho-D-arabinonhydrazide
P31072
-
5-phospho-D-arabinono-1,4-lactone
-
-
5-phospho-D-arabinono-1,4-lactone
P31072
-
5-phospho-D-arabinonohydroxamic acid
-
-
5-phospho-D-arabinonohydroxamic acid
P31072
-
5-phospho-D-arabinose
-
-
5-phospho-D-arabinose
P31072
-
5-phospho-D-ribonate
-
-
5-phospho-D-ribonate
P31072
-
5-phospho-D-ribonate
-
substrate analogue
6-deoxy-6-phosphono-D-gluconate
-
-
6-deoxy-6-phosphono-D-gluconate
P31072
-
6-methoxy-7-hydroxycoumarin
-
scopolin
6-phospho-D-allonate
-
-
6-phospho-D-galactonate
-
-
6-phospho-D-galactonate
P31072
-
6-phosphono-D-mannonate
-
competitive
6-phosphono-D-mannonate
P31072
competitive
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
NBD-Cl
acetic acid (1R,2R)-2-acetoxy-1-(acetylbenzyloxyaminocarbonyl)-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
compound shows in vitro antiparasitic activity
acetic acid (1R,2R)-2-acetoxy-1-(acetylbenzyloxyaminocarbonyl)-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
-
acetic acid (1R,2R)-2-acetoxy-1-(acetylbenzyloxyaminocarbonyl)-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
compound shows in vitro antiparasitic activity
acetic acid (1R,2R)-2-acetoxy-1-benzyloxycarbamoyl-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
compound shows in vitro antiparasitic activity
acetic acid (2R,1R)-2-acetoxy-1-hydroxycarbamoyl-3-phosphonooxypropyl ester
-
-
acetic acid (2R,1R)-2-acetoxy-1-hydroxycarbamoyl-3-phosphonooxypropyl ester
-
compound shows in vitro antiparasitic activity
adenosine
-
-
amikacin
-
in vitro inhibition
amikacin sulfate
-
in vitro and in vivo inhibition
amoxicillin
-
competitive
Ampicillin
-
in vitro inhibition
Ampicillin
-
in vitro inhibition
Butan-2,3-dione
-
-
caffeic acid
-
-
cefepime
-
in vitro inhibition
cefepime
-
in vitro and in vivo inhibition, noncompetitive
ceftazidim
-
in vitro inhibition
Chloramphenicol
-
in vitro inhibition
chlorogenic acid
-
-
Ciprofloxacin
-
in vitro inhibition
clindamycin
-
competitive
D-2-deoxyglucose
-
-
D-5-phosphoarabonate
-
-
D-5-phosphoribonate
-
-
D-5-phosphoribosyl-1-diphosphate
-
-
D-5-phosphoribosyl-1-diphosphate
-
-
D-6-phosphogluconate
-
competitive substrate inhibition at pH 7
D-6-sulfogluconate
-
-
D-6-sulfogluconate
-
-
D-erythrose 4-phosphate
-
-
D-erythrose 4-phosphate
-
-
D-erythrose 4-phosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-fructose 1,6-diphosphate
-
-
D-Fructose 1-phosphate
-
-
D-fructose 2,6-bisphosphate
-
-
D-fructose 6-phosphate
-
-
D-fructose 6-phosphate
-
-
D-Glucose 1,6-bisphosphate
-
-
D-Glucose 1,6-bisphosphate
-
-
D-Glucose 1,6-bisphosphate
-
-
D-glucose 6-phosphate
-
-
D-glyceraldehyde 3-phosphate
-
-
D-glyceraldehyde 3-phosphate
-
-
D-phosphate
-
competitive inhibitor of the substrates
-
D-phosphate
-
-
-
D-ribulose 5-phosphate
-
-
D-ribulose 5-phosphate
-
-
D-ribulose 5-phosphate
-
-
D-ribulose 5-phosphate
-
-
D-ribulose 5-phosphate
-
-
D-ribulose 5-phosphate
-
-
D-ribulose 5-phosphate
-
-
D-xylulose 5-phosphate
-
-
diethyldicarbonate
-
-
ferulic acid
-
-
gentamicin sulfate
-
in vitro inhibition
hexachloroplatinate
-
-
Imipenem
-
noncompetitive
iodoacetamide
-
-
isepamycin
-
in vitro inhibition
levofloxacin
-
in vitro inhibition
lornoxicam
-
noncompetitive
metronidazole
-
noncompetitive
Mg2+
-
acts as an inhibitor above 20 mM
N-ethylmaleimide
-
-
NADPH
-
product inhibition
NADPH
-
noncompetitive inhibition versus 6-phosphogluconate
NADPH
-
competitive inhibition
NADPH
-
competitive inhibition
NADPH
-
competitive inhibition
NADPH
-
competitive inhibition
NADPH
-
competitive inhibition versus NADP+ with 6-phospho-D-gluconate equal to its Km and at a saturating concentration
NADPH
-
saturating concentration at pH 7.5
NADPH
-
complete inhibition
NADPH
-
6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step
netilmicin sulfate
-
in vitro inhibition
netilmicin sulfate
-
in vitro and in vivo inhibition, competitive
netilmicin sulfate
-
in vitro and in vivo inhibition
Nicotinamide
-
-
ofloxacin
-
in vitro inhibition
ornidazole
-
noncompetitive
oxalate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-coumaric acid
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
penicillin G
-
in vitro inhibition
phosphoenolpyruvate
-
-
phosphoric acid dibenzyl ester (2R,3R)-3-benzyloxycarbamoyl-2,3-dihydroxypropyl ester
-
compound shows in vitro antiparasitic activity
phosphoric acid dibenzyl ester (4R,5R)-5-benzyloxycarbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl ester
-
compound shows in vitro antiparasitic activity
phosphoric acid mono-[(4R,5R)-5-carbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl] ester
-
-
phosphoric acid mono-[(4R,5R)-5-carbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl] ester
-
compound shows in vitro antiparasitic activity
phosphoric acid mono-[(5R,4R)-5-hydroxycarbamoyl-2,2-dimethyl[1,3]dioxolan-4-ylmethyl] ester
-
compound shows low in vitro antiparasitic activity
phosphoric acid mono-[(5R,4R)-5-hydroxycarbamoyl-2,2-dimethyl[1,3]dioxolan-4-ylmethyl] ester
-
-
phosphoric acid mono-[(5R,4R)-5-hydroxycarbamoyl-2,2-dimethyl[1,3]dioxolan-4-ylmethyl] ester
-
compound shows low in vitro antiparasitic activity
potassium 3,4-dioxo-3,4-dihydronaphthalene-1-sulfonate
-
-
pyridoxal 5'-phosphate
-
photooxidation
pyridoxal 5'-phosphate
-
photooxidation
pyridoxal 5'-phosphate
-
-
Rose bengal
-
photooxidation
sodium (4E)-4-(hydroxyimino)-3-oxo-1,2,3,4-tetrahydronaphthalene-1-sulfonate
-
-
sulfate
-
-
teicoplanin
-
in vitro inhibition
Tetranitromethane
-
irreversible loss of catalytic activity
Vancomycin
-
competitive
[(2R)-2,3-dihydroxypropylsulfanyl]acetic acid benzyl ester
-
compound shows in vitro antiparasitic activity
[(2R)-2-acetoxy-3-(bis(benzyloxy)phosphoryloxy)-propane-1-sulfinyl]acetic acid benzyl ester
-
compound shows in vitro antiparasitic activity
[(2R)-2-acetoxy-3-(bis(benzyloxy)phosphoryloxy)-propylsulfanyl]acetic acid
-
compound shows in vitro antiparasitic activity
[(2R)-2-acetoxy-3-phosphonooxypropane-1-sulfinyl]acetic acid
-
-
[(2R)-2-acetoxy-3-phosphonooxypropane-1-sulfinyl]acetic acid
-
compound shows in vitro antiparasitic activity
[(4R)-2,2-dimethyl-[1,3]dioxolan-4-ylmethylsulfanyl]acetic acid benzyl ester
-
compound shows low in vitro antiparasitic activity
[(5-nitrofuran-2-yl)methylidene]propanedioic acid
-
-
[3-(bis(benzyloxy)phosphoryloxy)-(2R)-2-hydroxypropane-1-sulfinyl]acetic acid
-
compound shows in vitro antiparasitic activity
[3-(bis(benzyloxy)phosphoryloxy)-(2R)-2-hydroxypropane-1-sulfinyl]acetic acid benzyl ester
-
compound shows in vitro antiparasitic activity
additional information
-
infection with potato virus Y increases the enzyme activity but decreases the 6-phosphono-D-gluconate content, maximal decrease of 30-50% at period of the highest virus replication
-
additional information
-
ovariectomized rats show a decrease in mRNA level and enzyme activity in the liver, but not in white adipose tissue, which can be restored by 17beta-estradiol administration
-
additional information
-
no inhibition by cefozin, decefin, streptomycin, combisid, and meronem
-
additional information
-
IC50 for antiparasitic activity of inhibitors, overview
-
additional information
-
no inhibition by [3-(bis(benzyloxy)phosphoryloxy)-(2R)-2-hydroxypropane-1-sulfinyl]acetic acid, [(2R)-2-acetoxy-3-(bis(benzyloxy)phosphoryloxy)-propane-1-sulfinyl]acetic acid benzyl ester, (2R)-[3-(bis(benzyloxy)phosphoryloxy)-2-(tetrahydropyran-2-yloxy)propane-1-sulfinyl]acetic acid, and acetic acid (1R,2R)-2-acetoxy-1-benzyloxycarbamoyl-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
additional information
-
IC50 for antiparasitic activity of inhibitors, overview
-
additional information
-
IC50 values for antiparasitic activity of inhibitors, overview
-
additional information
-
not inhibited by ungallated catechins like (-)-epigallocatechin, (-)-gallocatechin, (-)-epicatechin, (-)-catechin, and dehydroepiandrosterone
-
additional information
-
the activityand transcript level of 6PGDH decreases to 67% of its initial activity after 7 days of phosphate starvation
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
17beta-estradiol
-
administration causes an increase in liver enzyme, but not in white adipose tissue enzyme, activity and mRNA level in female and male rats
2,3-diphosphoglycerate
-
activates both isoenzymes up to 200% of their original activity
5-(3alpha-acetoxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
activates
5-(3alpha-hydroxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
activates
6-phosphogluconate
-
bona fide allosteric activator
AMP
-
1 mM, relative activity 105%
D-Fructose 1-phosphate
-
-
D-fructose 6-phosphate
-
slight activation
D-gluconate
-
induced by
gluconate
Q6NHC5
6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3-fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions
metimazole
-
in vitro activation
additional information
Q7FRX8
the enzyme is upregulated during salt stress
-
additional information
-
external gluconate has no effect on the activity of 6-phosphogluconate dehydrogenase
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0096
-
2-deoxy-3-oxo-6-phosphogluconate
-
-
0.082
-
2-deoxy-3-oxo-6-phosphogluconate
-
-
1.42
-
2-deoxy-3-oxo-6-phosphogluconate
-
-
0.55
-
2-deoxy-6-phosphogluconate
-
-
1.4
-
2-deoxy-6-phosphogluconate
-
-
1.42
-
2-deoxy-6-phosphogluconate
-
-
0.0048
-
6,7-dideoxy-7-phosphono-D-glucoheptonate
P31072
pH 8.0, 20C
0.011
-
6,7-dideoxy-7-phosphono-D-glucoheptonate
-
pH 8.0, 20C
0.00013
-
6-phospho-D-gluconate
-
-
0.0102
-
6-phospho-D-gluconate
-
pH 7.6, 25C
0.011
-
6-phospho-D-gluconate
-
in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80C
0.0121
-
6-phospho-D-gluconate
-
mutant enzyme H186A
0.024
-
6-phospho-D-gluconate
-
mutant enzyme T262A
0.028
-
6-phospho-D-gluconate
-
wild type enzyme
0.0284
-
6-phospho-D-gluconate
-
wild type enzyme
0.04
-
6-phospho-D-gluconate
-
dimeric enzyme with two 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication
0.0509
-
6-phospho-D-gluconate
P38720
pH 7.5
0.07
-
6-phospho-D-gluconate
-
dimeric enzyme with one 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication
0.093
-
6-phospho-D-gluconate
P00350
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25C
0.095
-
6-phospho-D-gluconate
-
mutant enzyme S128A
0.107
-
6-phospho-D-gluconate
Q762L5
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25C
0.15
-
6-phospho-D-gluconate
-
mutant enzyme K260A
0.53
-
6-phospho-D-gluconate
-
mutant enzyme N187A
0.595
-
6-phospho-D-gluconate
-
-
1.04
-
6-phospho-D-gluconate
-
mutant enzyme Y191A
2
3
6-phospho-D-gluconate
-
mutant enzyme R287A
3.4
-
6-phospho-D-gluconate
-
mutant enzyme R446A
0.006
-
6-phosphogluconate
-
-
0.0068
-
6-phosphogluconate
-
pH 10
0.0069
-
6-phosphogluconate
-
-
0.007
-
6-phosphogluconate
-
-
0.0096
-
6-phosphogluconate
-
proplastid isoenzyme
0.01
-
6-phosphogluconate
-
-
0.011
-
6-phosphogluconate
-
-
0.012
-
6-phosphogluconate
-
cytosolic isoenzyme
0.0135
-
6-phosphogluconate
-
-
0.014
-
6-phosphogluconate
-
60C or 65C
0.0149
-
6-phosphogluconate
-
50 mM Tris-acetate, 5-10 mM MgCl2, pH 7.7
0.015
-
6-phosphogluconate
-
-
0.015
-
6-phosphogluconate
-
cytosolic isoenzyme
0.016
-
6-phosphogluconate
-
-
0.016
-
6-phosphogluconate
-
30C
0.016
-
6-phosphogluconate
-
-
0.0161
-
6-phosphogluconate
-
50 mM Tris-acetate, pH 7.7
0.0167
-
6-phosphogluconate
-
10 mM Tris-acetate, 80 mM KCl, pH 7.7
0.018
-
6-phosphogluconate
-
plastid isoenzyme
0.02
-
6-phosphogluconate
-
43C
0.02
-
6-phosphogluconate
-
-
0.022
-
6-phosphogluconate
-
45C
0.0227
-
6-phosphogluconate
-
-
0.023
-
6-phosphogluconate
-
-
0.024
-
6-phosphogluconate
-
-
0.02666
-
6-phosphogluconate
-
-
0.031
-
6-phosphogluconate
-
type II
0.034
-
6-phosphogluconate
-
-
0.0343
-
6-phosphogluconate
-
-
0.035
-
6-phosphogluconate
-
-
0.036
-
6-phosphogluconate
-
-
0.04
0.05
6-phosphogluconate
-
heterocaryon col-3-wild-type
0.04
-
6-phosphogluconate
-
-
0.04
-
6-phosphogluconate
-
-
0.0413
-
6-phosphogluconate
-
10 mM Tris-acetate, pH 7.7
0.043
-
6-phosphogluconate
-
-
0.045
-
6-phosphogluconate
-
-
0.05
-
6-phosphogluconate
-
50 mM Tris-acetate, 20 mM MgCl2, KCl, pH 7.7
0.05
-
6-phosphogluconate
-
heterocaryon col-10-wild-type
0.05
-
6-phosphogluconate
-
-
0.052
-
6-phosphogluconate
-
type I
0.054
-
6-phosphogluconate
-
-
0.054
-
6-phosphogluconate
-
-
0.06
-
6-phosphogluconate
-
col-3-col-10
0.065
-
6-phosphogluconate
-
-
0.068
-
6-phosphogluconate
-
pH 5.93
0.071
-
6-phosphogluconate
-
-
0.071
-
6-phosphogluconate
-
source embryo
0.071
-
6-phosphogluconate
-
pH 7
0.08
-
6-phosphogluconate
-
isoenzyme 2
0.085
-
6-phosphogluconate
-
pH 8
0.09
-
6-phosphogluconate
-
mutant col 3
0.09
-
6-phosphogluconate
-
-
0.098
-
6-phosphogluconate
-
-
0.1
-
6-phosphogluconate
-
isoenzyme 1
0.109
-
6-phosphogluconate
-
plastid isoenzyme
0.11
-
6-phosphogluconate
-
mutant col 10
0.11
-
6-phosphogluconate
-
-
0.11
-
6-phosphogluconate
-
-
0.153
-
6-phosphogluconate
-
pH 8.0
0.157
-
6-phosphogluconate
-
-
0.204
-
6-phosphogluconate
-
pH 9
0.22
-
6-phosphogluconate
-
-
0.29
-
6-phosphogluconate
-
-
0.313
-
6-phosphogluconate
-
23.5C
0.402
-
6-phosphogluconate
-
source endosperm
0.64
-
6-phosphogluconate
-
pH 9.5
3.13
-
6-phosphogluconate
-
pH 9.03
0.0035
-
6-phosphono-D-gluconate
-
pH 7.5, 20C
0.0053
-
6-phosphono-D-gluconate
P31072
pH 8.0, 20C
0.016
-
6-phosphono-D-gluconate
-
pH 7.5, 20C
0.022
-
6-phosphono-D-gluconate
Q6WAT5
pH 7.5, 30C, purified native wild-type enzyme
0.0222
-
6-phosphono-D-gluconate
Q6WAT5
pH 7.5, 30C, purified recombinant mutant enzyme
0.03
-
6-phosphono-D-gluconate
-
pH 8.0, 20C
34
-
CO2
-
-
1.7
-
MgCl2
-
cytosolic isoenzyme
2.4
-
MgCl2
-
proplastid isoenzyme
0.38
-
NAD+
-
in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80C
0.00088
-
NADP+
-
-
0.002
-
NADP+
-
wild type enzyme
0.002
-
NADP+
-
mutant enzyme H186A
0.0041
-
NADP+
-
cytosolic isoenzyme
0.0042
-
NADP+
-
mutant enzyme T262A
0.0046
-
NADP+
Q6WAT5
pH 7.5, 30C, purified native wild-type enzyme
0.005
-
NADP+
-
wild type enzyme
0.0054
-
NADP+
-
proplastid isoenzyme
0.0054
-
NADP+
-
mutant enzyme S128A
0.00571
-
NADP+
-
10 mM Tris-acetate, 10 mM MgCl2, pH 7.7
0.0059
-
NADP+
Q6WAT5
pH 7.5, 30C, purified recombinant mutant enzyme
0.006
-
NADP+
-
source endosperm
0.00631
-
NADP+
-
10 mM Tris-acetate, 80 mM KCl, pH 7.7
0.0064
-
NADP+
-
mutant enzyme M13C
0.00676
-
NADP+
-
50 mM Tris-acetate, pH 7.7
0.0068
-
NADP+
-
-
0.007
-
NADP+
-
-
0.007
-
NADP+
-
-
0.008
-
NADP+
-
cytosolic isoenzyme
0.00861
-
NADP+
-
50 mM Tris-acetate, 20 mM MgCl2, pH 7.7
0.00893
-
NADP+
-
10 mM Tris-acetate, pH 7.7
0.01
-
NADP+
-
-
0.01
-
NADP+
-
wild-type, mutant col 10, heterocaryon col-10-wild-type
0.01
-
NADP+
-
in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80C
0.011
-
NADP+
-
plastid isoenzyme
0.011
-
NADP+
-
-
0.013
-
NADP+
-
-
0.013
-
NADP+
-
-
0.014
-
NADP+
-
mutant enzyme Y191A
0.015
-
NADP+
-
heterocaryon col 3-wild-type
0.015
-
NADP+
-
-
0.016
-
NADP+
-
-
0.016
-
NADP+
-
-
0.016
-
NADP+
-
mutant enzyme K260A
0.018
-
NADP+
-
heterocaryon col-3-col-10
0.019
-
NADP+
-
mutant enzyme M13F; mutant enzyme M13Q
0.02
-
NADP+
-
type I and II
0.0217
-
NADP+
-
23.5C
0.023
-
NADP+
-
plastid isoenzyme
0.025
-
NADP+
-
source of enzyme: peak I
0.025
-
NADP+
-
pH 8.0
0.025
-
NADP+
-
-
0.026
-
NADP+
-
mutant enzyme M13V
0.029
-
NADP+
-
-
0.0298
-
NADP+
-
mutant enzyme N187A
0.03
-
NADP+
-
source of enzyme: whole cells
0.03
-
NADP+
-
mutant col-3
0.03
-
NADP+
-
-
0.03
-
NADP+
-
-
0.03
-
NADP+
-
-
0.03
-
NADP+
-
-
0.031
-
NADP+
-
pH 9.03
0.032
-
NADP+
-
source embryo
0.032
-
NADP+
-
mutant enzyme M13I
0.033
-
NADP+
-
source of enzyme: peak II
0.033
-
NADP+
-
pH 5.93
0.035
-
NADP+
P38720
pH 7.5
0.049
-
NADP+
P00350
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25C
0.053
-
NADP+
-
-
0.064
-
NADP+
-
mutant enzyme R446A
0.073
-
NADP+
-
mutant enzyme R287A
0.118
-
NADP+
Q762L5
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25C
0.258
-
NADP+
-
-
0.6211
-
NADP+
-
-
0.00022
-
NADPH
-
-
0.02
-
ribulose-5-phosphate
-
-
1
-
ribulose-5-phosphate
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.53
-
6,7-dideoxy-7-phosphono-D-glucoheptonate
-
pH 8.0, 20C
1.4
-
6,7-dideoxy-7-phosphono-D-glucoheptonate
P31072
pH 8.0, 20C
0.217
-
6-phosphogluconate
-
-
9
-
6-phosphono-D-gluconate
-
pH 8.0, 20C
31.8
-
6-phosphono-D-gluconate
P31072
pH 8.0, 20C
98
-
6-phosphono-D-gluconate
Q6WAT5
pH 7.5, 30C, purified native wild-type enzyme
98
-
NADP+
Q6WAT5
pH 7.5, 30C, purified native wild-type enzyme
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.198
-
(2R)-(2-hydroxy-3-phosphonooxypropane-1-sulfinyl)acetic acid
-
pH 7.5, 20C
0.784
-
(2R)-(2-hydroxy-3-phosphonooxypropane-1-sulfinyl)acetic acid
-
pH 7.5, 20C
3.14
-
(4S)-trans-4-ethylammonio-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
-
0.274
-
1,5-diphospho-D-ribulose
P31072
pH 8.0, 20C
1
-
1,5-diphospho-D-ribulose
-
pH 8.0, 20C
0.122
-
1,5-diphospho-ribitol
P31072
pH 8.0, 20C
0.5
-
1,5-diphospho-ribitol
-
pH 8.0, 20C
0.0044
-
2-deoxy-6-phosphono-D-gluconate
P31072
pH 8.0, 20C
0.0044
-
2-deoxy-6-phosphono-D-gluconate
-
pH 7.5, 20C
0.77
-
2-deoxy-6-phosphono-D-gluconate
-
pH 8.0, 20C
0.77
-
2-deoxy-6-phosphono-D-gluconate
-
pH 7.5, 20C
0.0015
-
4-phopho-D-erythronamide
-
pH 7.5, 20C
0.039
-
4-phospho-D-erythronamide
-
pH 7.5, 20C
0.00013
-
4-phospho-D-erythronate
P31072
pH 8.0, 20C
0.00013
-
4-phospho-D-erythronate
-
pH 7.5, 20C
0.00013
-
4-phospho-D-erythronate
-
-
0.011
-
4-phospho-D-erythronate
-
pH 8.0, 20C
0.011
-
4-phospho-D-erythronate
-
pH 7.5, 20C
0.00001
-
4-phospho-D-erythronohydroxamic acid
-
pH 7.5, 20C
0.0025
-
4-phospho-D-erythronohydroxamic acid
-
pH 7.5, 20C
0.175
-
4-phospho-D-erythrose
P31072
pH 8.0, 20C
1.59
-
4-phospho-D-erythrose
-
pH 8.0, 20C
0.0212
-
5,6-dideoxy-6-phosphono-D-arabino-hexonate
P31072
pH 8.0, 20C
1
-
5,6-dideoxy-6-phosphono-D-arabino-hexonate
-
pH 8.0, 20C
0.0042
-
5-(3alpha,7alpha,12alpha-triacetoxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
-
0.0878
-
5-(3alpha,7alpha,12alpha-trihydroxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
-
0.007
-
5-deoxy-5-phosphonoD-arabinonate
P31072
pH 8.0, 20C
0.433
-
5-deoxy-5-phosphonoD-arabinonate
-
pH 8.0, 20C
0.0056
-
5-phospho-D-arabinonamide
P31072
pH 8.0, 20C
0.0803
-
5-phospho-D-arabinonamide
-
pH 8.0, 20C
0.0034
-
5-phospho-D-arabinonate
P31072
pH 8.0, 20C
0.109
-
5-phospho-D-arabinonate
-
pH 8.0, 20C
0.0267
-
5-phospho-D-arabinonhydrazide
P31072
pH 8.0, 20C
0.128
-
5-phospho-D-arabinonhydrazide
-
pH 8.0, 20C
0.011
-
5-phospho-D-arabinono-1,4-lactone
P31072
pH 8.0, 20C
0.147
-
5-phospho-D-arabinono-1,4-lactone
-
pH 8.0, 20C
0.0058
-
5-phospho-D-arabinonohydroxamic acid
P31072
pH 8.0, 20C
0.307
-
5-phospho-D-arabinonohydroxamic acid
-
pH 8.0, 20C
0.025
-
5-phospho-D-arabinose
P31072
pH 8.0, 20C
0.71
-
5-phospho-D-arabinose
-
pH 8.0, 20C
0.00095
-
5-phospho-D-ribonate
P31072
pH 8.0, 20C
0.00095
-
5-phospho-D-ribonate
-
-
0.067
-
5-phospho-D-ribonate
-
pH 8.0, 20C
0.0013
-
6-deoxy-6-phosphono-D-gluconate
P31072
pH 8.0, 20C
0.0358
-
6-deoxy-6-phosphono-D-gluconate
-
pH 8.0, 20C
0.19
-
6-phospho-D-galactonate
P31072
pH 8.0, 20C
0.565
-
6-phospho-D-galactonate
-
pH 8.0, 20C
0.135
-
6-phosphono-D-mannonate
-
pH 8.0, 20C
0.8
-
6-phosphono-D-mannonate
P31072
pH 8.0, 20C
0.109
-
acetic acid (1R,2R)-2-acetoxy-1-(acetylbenzyloxyaminocarbonyl)-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
pH 7.5, 20C
0.192
-
acetic acid (1R,2R)-2-acetoxy-1-(acetylbenzyloxyaminocarbonyl)-3-(bis(benzyloxy)phosphoryloxy)propyl ester
-
pH 7.5, 20C
0.00008
-
acetic acid (2R,1R)-2-acetoxy-1-hydroxycarbamoyl-3-phosphonooxypropyl ester
-
pH 7.5, 20C
0.00036
-
acetic acid (2R,1R)-2-acetoxy-1-hydroxycarbamoyl-3-phosphonooxypropyl ester
-
pH 7.5, 20C
287.6
-
amoxicillin
-
25C, pH 8.0
2.579
-
cefepime
-
pH 8.0, 25C
130.2
-
clindamycin
-
25C, pH 8.0
0.77
-
Imipenem
-
25C, pH 8.0
0.4
-
lornoxicam
-
25C, pH 8.0
0.57
-
metronidazole
-
25C, pH 8.0
0.00065
-
NADPH
-
mutant enzyme M13C, at a saturating concentration
0.001
-
NADPH
-
mutant enzyme M13I, at a saturating concentration
0.0011
-
NADPH
-
wild type enzyme, at a saturating concentration
0.0015
-
NADPH
-
mutant enzyme M13F, at a saturating concentration
0.0021
-
NADPH
-
mutant enzyme M13Q, at a saturating concentration
0.006
-
NADPH
-
mutant enzyme M13V, at a saturating concentration
0.008
-
NADPH
-
wild type enzyme, at a saturating concentration of NADPH at pH 7.5
0.0093
-
NADPH
-
mutant enzyme N187A, at a saturating concentration of NADPH at pH 7.5
0.019
-
NADPH
-
mutant enzyme S128A, at a saturating concentration of NADPH at pH 7.5
0.027
-
NADPH
-
mutant enzyme H186A, at a saturating concentration of NADPH at pH 7.5
0.03191
-
NADPH
-
competitive inhibitor
0.939
-
netilmicin
-
pH 8.0, 25C
42.4
-
ornidazole
-
25C, pH 8.0
0.588
-
phosphoric acid mono-[(4R,5R)-5-carbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl] ester
-
pH 7.5, 20C
0.714
-
phosphoric acid mono-[(4R,5R)-5-carbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl] ester
-
pH 7.5, 20C
0.000035
-
phosphoric acid mono-[(5R,4R)-5-hydroxycarbamoyl-2,2-dimethyl[1,3]dioxolan-4-ylmethyl] ester
-
pH 7.5, 20C
0.0011
-
phosphoric acid mono-[(5R,4R)-5-hydroxycarbamoyl-2,2-dimethyl[1,3]dioxolan-4-ylmethyl] ester
-
pH 7.5, 20C
65.6
-
Vancomycin
-
25C, pH 8.0
0.04
-
[(2R)-2-acetoxy-3-phosphonooxypropane-1-sulfinyl]acetic acid
-
pH 7.5, 20C
0.625
-
[(2R)-2-acetoxy-3-phosphonooxypropane-1-sulfinyl]acetic acid
-
pH 7.5, 20C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00128
-
(-)-catechin gallate
-
in 0.1 M Tris buffer (pH 7.5), 150 mM NaCl, 6 mM MgCl2, at 30C
0.00121
-
(-)-epicatechin gallate
-
in 0.1 M Tris buffer (pH 7.5), 150 mM NaCl, 6 mM MgCl2, at 30C
0.00072
-
(-)-epigallocatechin gallate
-
in 0.1 M Tris buffer (pH 7.5), 150 mM NaCl, 6 mM MgCl2, at 30C
0.00145
-
(-)-gallocatechin gallate
-
in 0.1 M Tris buffer (pH 7.5), 150 mM NaCl, 6 mM MgCl2, at 30C
0.0058
-
(2E)-2-cyano-3-(5-nitrofuran-2-yl)prop-2-enoic acid
-
pH 7.0, temperature not specified in the publication
1.41
-
(4S)-trans-4-ethylammonio-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
-
0.028
-
2-methyl-5-nitrofuran-3-carboxylic acid
-
pH 7.0, temperature not specified in the publication
0.114
-
3,3',4,4'-tetramethyl-1H,1'H-2,2'-bipyrrole-5,5'-dicarboxylic acid
-
pH 7.0, temperature not specified in the publication
0.051
-
3-methyl-4-oxo-4,7-dihydro-5H-thiopyrano[3,4-b]furan-2-carboxylic acid
-
pH 7.0, temperature not specified in the publication
0.042
-
4,5-dichloro-1H-pyrrole-2-carboxylic acid
-
pH 7.0, temperature not specified in the publication
0.043
-
4-chloro-1H-pyrrole-2-carboxylic acid
-
pH 7.0, temperature not specified in the publication
0.042
-
4H-thieno[3,2-b]pyrrole-5-carboxylic acid
-
pH 7.0, temperature not specified in the publication
0.00253
-
5-(3alpha,7alpha,12alpha-triacetoxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
-
0.0601
-
5-(3alpha,7alpha,12alpha-trihydroxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
-
0.045
-
5-(ethoxycarbonyl)-3,4-dimethyl-1H-pyrrole-2-carboxylic acid
-
pH 7.0, temperature not specified in the publication
287.4
-
amoxicillin
-
25C, pH 8.0
117.4
-
clindamycin
-
25C, pH 8.0
0.43
-
Imipenem
-
25C, pH 8.0
0.17
-
lornoxicam
-
25C, pH 8.0
0.23
-
metronidazole
-
25C, pH 8.0
21.79
-
ornidazole
-
25C, pH 8.0
0.053
-
potassium 3,4-dioxo-3,4-dihydronaphthalene-1-sulfonate
-
pH 7.0, temperature not specified in the publication
0.073
-
sodium (4E)-4-(hydroxyimino)-3-oxo-1,2,3,4-tetrahydronaphthalene-1-sulfonate
-
pH 7.0, temperature not specified in the publication
46.39
-
Vancomycin
-
25C, pH 8.0
0.056
-
[(5-nitrofuran-2-yl)methylidene]propanedioic acid
-
pH 7.0, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.025
-
Q6NHC5
activity of 6-phosphogluconate dhydrogenase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37C, supplemented with glucoronate
0.032
-
-
supernantant
0.094
-
Q6NHC5
activity of 6-phosphogluconate dhydrogenase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37C, supplemented with 0.5% fructose
0.1207
-
-
kidney enzyme, optimum conditions, pH 8.0, 37C
0.152
-
Q6NHC5
activity of 6-phosphogluconate dhydrogenase expressed in transformed Escherichia coli mutants, supplemented with 0.5% fructose as carbon source
0.177
-
-
homogenate
0.291
-
Q6NHC5
activity of 6-phosphogluconate dhydrogenase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37C, supplemented with 0.5% gluconate
0.41
-
-
-
0.4242
-
-
liver enzyme, optimum conditions, pH 8.0, 37C
1.33
-
-
-
1.89
-
-
supernatant fraction
1.89
-
-
purified enzyme
4.7
-
-
recombinant enzyme
12
-
-
total enzyme after gel filtration in presence of 6-phosphogluconate
15
-
-
-
15.12
-
-
after 473fold purification
17.4
-
-
-
18.7
-
-
ultrafiltration
19
-
-
pure enzyme
21
-
-
-
21.2
-
-
-
22
-
-
150fold purification
25.75
-
-
-
32
-
Q6WAT5
purified recombinant enzyme
33.5
-
-
total enzyme after gel filtration in absence of 6-phosphogluconate
38
-
-
cytoplasmic isoenzyme
61
-
-
after 344fold purification
80
-
-
pH 7.5, 25C
92.3
-
-
-
175
-
-
cell-free extract
additional information
-
-
plastid isoenzyme 4.8 nkat mg-1 protein, cytosolic isoenzyme 2.5 nkat mg-1 protein
additional information
-
-
activity in healthy and infected leaves during 9 days
additional information
-
-
6-phosphogluconate dehydrogenase activity in not enhanced in inoculated mesquite plants incubated for 15 or 23 days
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.8
7.2
-
decarboxylation in absence of activators
6.9
-
-
assay at
7
9
-
both isoenzymes
7
-
-
assay at
7.5
-
-
in 50 mM Tris-HCl
7.6
-
-
assay at
7.7
-
-
low substrate concentrations
7.8
8
-
cytosolic and proplastid isoenzymes
8
-
-
liver and kidney cortex enzyme
8
-
-
6-phosphogluconate oxidation
8
-
-
assay at
8
-
P31072
assay at
8
-
-
assay at
8.3
-
-
Tris-Cl orbicine-acetate buffers
8.4
-
Q6WAT5
-
8.5
-
-
-
8.6
8.8
-
both Tris and tricine buffers, more active in tricine buffers
9
-
-
isoenzyme 1, chloroplasts
9
-
-
6-phosphogluconate-activated decarboxylation
9.1
-
-
-
9.1
-
-
reduction
9.3
-
-
isoenzyme 2, cytoplasm
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
9
-
-
5.5
9.1
-
-
6
9
-
about 70% of 6PGDH activities remain at pH 6.0 and 9.0
7.5
10
-
-
7.5
8.5
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
-
-
assay at
20
-
P31072
assay at
24
-
-
assay at
25
-
-
maximum activity at pH 8 also at 43C
25
-
-
assay at
30
-
Q6WAT5
assay at
37
-
Q6NHC5
assay at
43
-
-
maximum activity at pH 8 also at 25C
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
9
-
with increasing phosphate buffer concentration from 25 to 150 mM
20
95
-
the enzyme has an approximately 90% of its maximum activity at 80C, and retains about 2 and about 20% of its maximum activity at 30 and 60C, respectively
25
70
-
retains full activity at 60C
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.1
-
-
isoelectric focusing, pH-gradient: 3.5-7.0
6.6
-
-
calculated from sequence
6.9
-
Q6WAT5
electric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
a 6PGD gene expression pattern in ovines differing in weight is not followed by analogous changes of its enzymatic activity suggesting that other post transcription or post translation regulation is involved
Manually annotated by BRENDA team
-
low activity in female and male rats, constant at age 2 months to age 9 months
Manually annotated by BRENDA team
-
high activity in female and male rats, decreasing from age 2 months to age 9 months
Manually annotated by BRENDA team
Q7FRX8
low enzyme expression level
Manually annotated by BRENDA team
-
low activity in female and male rats, constant at age 2 months to age 9 months
Manually annotated by BRENDA team
-
nicotine has no effect on enzyme activity
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
Q7FRX8
high enzyme expression level
Manually annotated by BRENDA team
-
cortex, low activity in female and male rats, decreasing from age 2 months to age 9 months
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
cortex
-
Manually annotated by BRENDA team
Q7FRX8
very low enzyme expression level
Manually annotated by BRENDA team
Nicotiana tabacum W-38
-
-
-
Manually annotated by BRENDA team
-
moderate activity in female and male rats, decreasing from age 2 months to age 9 months
Manually annotated by BRENDA team
-
nicotine has no effect on enzyme activity. Vitamin E may favourably increase 6PGD activity
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley, Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
-
nicotine activates enzyme activity, increase in activity is eliminated by vitamin E
Manually annotated by BRENDA team
-
leg and skeletal
Manually annotated by BRENDA team
-
nicotine activates enzyme activity, when administered with vitamin E the increase in enzyme activity is lower
Manually annotated by BRENDA team
Q7FRX8
low enzyme expression level
Manually annotated by BRENDA team
Q7FRX8
3-leaf stage
Manually annotated by BRENDA team
-
transcript levels of both Os6PGDH1 and Os6PGDH2 are up-regulated in rice seedlings under drought, cold, high salinity and abscisic acid treatments
Manually annotated by BRENDA team
Q7FRX8
the enzyme is upregulated during salt stress
Manually annotated by BRENDA team
-
low activity in female and male rats, constant at age 2 months to age 9 months
Manually annotated by BRENDA team
-
antibodies to 6-phosphogluconate dehydrogenase, decarboxylating are present in serum samples of ASA-positive (antisperm antibodies) infertile men
Manually annotated by BRENDA team
-
nicotine activates enzyme activity, when administered with vitamin E the increase in enzyme activity is lower
Manually annotated by BRENDA team
-
moderate activity in female and male rats, decreasing from age 2 months to age 9 months
Manually annotated by BRENDA team
additional information
-
enzyme mRNA concentration show age- and sex-related differences, especially in the liver, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Catharanthus roseus B/S
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley, Synechococcus sp. PCC 6307, Synechococcus sp. PCC 6716
-
-
-
Manually annotated by BRENDA team
Q7FRX8
cytosolic isozyme
Manually annotated by BRENDA team
-
dual localization to cytosol (95%) and peroxisomes
Manually annotated by BRENDA team
Catharanthus roseus B/S, Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
-
dual localization to cytosol and peroxisomes
Manually annotated by BRENDA team
Catharanthus roseus B/S
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
53000
-
-
SDS-PAGE
53400
-
-
SDS-PAGE, HAT-tagged r6PGD fusion protein
60000
-
-
gel filtration
69000
72000
-
2 isoenzymes, gel filtration
79000
-
-
gel filtration of haemolysates
83000
-
-
gel filtration, method of Ornstein and Davis
90000
-
-
gel filtration, Bio-Gel 200
92000
-
-
gel filtration, PAGE
93000
-
Q6WAT5
recombinant enzyme, gel filtration
94000
-
-
gel filtration, in presence of MgCl2
94000
-
-
gel filtration, sedimentation equlibrium
94000
-
-
-
95000
-
-
gel filtration
98000
-
-
sedimentation equilibrium
100000
-
-
sedimentation equilibrium
100000
-
-
gel filtration
100000
-
-
sucrose density gradient centrifugation
100000
-
-
gel filtration
100000
-
-
sedimentation equilibrium; sucrose density gradient centrifugation
101000
-
-
gel filtration
101000
-
-
sucrose density gradient centrifugation
101000
-
-
type I, centrifugation in sucrose density gradient
102000
-
-
gel filtration
102000
-
-
-
104000
-
-
-
104000
-
-
-
104000
-
-
sedimentation equilibrium, gel filtration
104000
-
-
-
104000
-
-
sedimentation equilibrium, gel filtration
104000
-
-
-
104000
-
-
gel filtration
104000
-
-
-
104000
-
-
gel filtration
104000
-
-
-
104000
-
-
gel filtration
104000
-
-
SDS-PAGE
106000
115000
-
gel filtration
106000
115000
-
-
108000
-
-
sedimentation equilibrium
110000
120000
-
gel filtration
110000
120000
-
-
110000
-
-
both isoenzymes
110000
-
-
-
110000
-
-
gel filtration
111000
-
-
sucrose density gradient centrifugation
111000
-
-
type II
112000
-
-
-
129000
-
-
-
129000
-
-
sedimentation equilibrium, short column method
129000
-
-
-
129000
-
-
presence of impurities
158000
-
-
gel filtration
229000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
A4VX60
x * 52400, calculated from sequence
?
-
x * 60000, spliced Gnd1-3xHA isoform, SDS-PAGE; x * 63000, alternatively spliced Gnd1-3xHA isoform, SDS-PAGE
dimer
-
heteromultimeric molecule composed of 2 nonidentical subunits
dimer
-
2 * 51000, SDS-PAGE
dimer
-
2 * 50000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE; 2 * 54000, maleic anhydride electrophoresis
dimer
-
2 * 50000, SDS-PAGE
dimer
-
SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
dimer
-
2 * 57000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
dimer
-
2 * 47000, SDS-PAGE
dimer
-
2 * 50000, type I, SDS-PAGE
dimer
-
2 * 54000, cytosolic isoenzyme, SDS-PAGE; 2 * 55000, SDS-PAGE
dimer
-
2 * 50000
dimer
-
1 * 49000 + 1 * 45000, SDS-PAGE
dimer
-
2 * 50000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
dimer
-
2 * 50000; 2 * 52000
dimer
-
2 * 51000, SDS-PAGE
dimer
-
2 * 58000, SDS-PAGE
dimer
Q6WAT5
2 * 51980, sequence determination, 2 * 51400, recombinant enzyme, SDS-PAGE
dimer
-
2 * 30000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE
dimer
Escherichia coli JM109 M15, Escherichia coli MRE 600
-
-
-
dimer
Geobacillus stearothermophilus NCA 1503
-
; 2 * 50000, SDS-PAGE; 2 * 50000, SDS-PAGE; 2 * 51000, SDS-PAGE
-
dimer
Rattus norvegicus Sprague-Dawley
-
2 * 52000, SDS-PAGE
-
dimer
Rattus norvegicus Wistar
-
-
-
homodimer
-
x-ray crystallography
homodimer
P00350
x-ray crystallography
homodimer
Q762L5
x-ray crystallography
tetramer
-
4 * 57000, SDS-PAGE
tetramer
-
4 * 38000, SDS-PAGE
tetramer
Arthrobacter globiformis VKM B-175
-
4 * 57000, SDS-PAGE
-
homodimer
Escherichia coli K12, Klebsiella pneumoniae NTUH-K2044
-
x-ray crystallography
-
additional information
Q6WAT5
three-dimensional structure modelling, low stability due to absence of inter-subunit salt bridges as compared to the enzyme from Trypanosoma brucei, the monomeric enzyme is inactive
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, using 0.1 M sodium citrate dihydrate buffer (pH5.4), 0.5 M ammonium acetate, 6-7% (w/v) PEG 3350, and 17-18% (w/v) PEG 4000 as a reservoir, at 18C
P00350
6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate, hanging drop vapour diffusion method, using 0.2 M lithium sulfate, 2.2 M ammonium sulfate or 0.2 M ammonium acetate, 2.2 M ammonium sulfate
-
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, at 4C, with 0.12 M diammonium hydrogen citrate buffer (pH 5.0) and 20% (w/v) polyethylene glycol (PEG) 3350
Q762L5
crystal structures of recombinant Lactococcus lactis 6-phosphogluconate dehydrogenase in complex with substrate, cofactor, product and inhibitors
-, P96789
hanging drop vapour diffusion method at 15C, crystal structure of 6-phosphogluconate dehydrogenase determined at 2.37 resolution by molecular replacement
P38720
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
9.5
-
mutant Y191A measured by varying 6PG saturation level of NADP+
8.4
9.8
Q6WAT5
purified native wild-type enzyme, loss of 30% activity within 10 min
8.5
-
-
the enzyme is stable at pH 8.5
9.1
-
-
unstable at higher pH values
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45
-
-
stable below
50
-
-
stable for at least 120 min
50
-
-
6-phosphogluconate increases heat stability
50
-
-
stable below, retains 50% original activity at 57C
60
-
-
stable at 60C
60
-
-
retains more than 90% of its structure
60
-
-
retains full activity after heating for 15 min
60
-
-
stable at optimal growth temperature
70
-
-
stable at relatively high temperatures
70
-
-
denaturation half-time of 80 min
80
90
-
the enzyme is highly thermostable in a 50 mM HEPES buffer (pH 6.8) containing 500 mM NaCl, 1 mM EDTA, and 5 mM beta-mercaptoethanol, it retains more than 90% activity for 48 h at 60 and 80C and retains about 50% enzymatic activity after 48 h at 90C, the enzyme has half-life times of 48 and 140 h at 90 and 80C, respectively
80
-
-
denaturation half-time of 3 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
stabilized by KCl
-
denaturation by urea
-
noteworthy stability to heat and protein denaturants
-
remarkably stable to the action of trypsin, chymotrypsin and elastase
-
stable in 8 M urea, nearly unaffected, only 10% loss of activity after exposure for 60 min
-
stable in 1 mM urea
-
both isoenzymes stabilized by the H2PO4-
-
enzyme loses no activity on dialysis overnight against 10 mM EDTA
-
a single step of freezing and thawing reduces the enzyme activity by 90%
Q6WAT5
low stability due to absence of inter-subunit salt bridges as compared to the enzyme from Trypanosoma brucei
Q6WAT5
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acetone
-
enzyme shows 15-20% enhanced activity
Acetone
Geobacillus stearothermophilus NCA 1503
-
enzyme shows 15-20% enhanced activity
-
dimethylformamide
-
enzyme shows 15-20% enhanced activity
dimethylformamide
Geobacillus stearothermophilus NCA 1503
-
enzyme shows 15-20% enhanced activity
-
dioxane
-
enzyme shows 15-20% enhanced activity
dioxane
Geobacillus stearothermophilus NCA 1503
-
enzyme shows 15-20% enhanced activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, purified enzyme in 50% glycerol buffer remains active for at least 3 months
-
4C, stable for several days, as an (NH4)2SO4 suspension stable for several months without any loss of enzyme activity
-
0C, stable to freezing and thawing, incubated for 24 h retains at least 70% of its original activity
-
5C, purified enzyme is stable to storage in 50 mM Tris HCl buffer, pH 7.5, 10 mM dithiothreitol and 20% glycerol, no significant decrease in activity occurs over a 2 week period
-
20C, in phosphate buffer pH 6.8 in presence of the reducing agent beta-mercaptoethanol enzyme remains fully active for 24 h
-
4C, retains full activity for several months when stored as an ammonium sulfate suspension or in acetate buffer, pH 6 in presence of a crystal of thymol to prevent bacterial growth
-
4C, stable for 5 h, loses 20% of its activity after 24%, no activity after 3 days
-
4C, the isoenzyme with an isolectric point of 6.5 retains virtually all its activity after storing for 30 days, the isoenzyme with isoelectric point 6.3 loses about 90% activity after storage for 3 days
-
5C, purified enzyme is stable up to 3 months if care is taken to exclude molecular oxygen from the enzyme solution
-
4C, 50 mM triethanolamine and 2 mM beta-mercaptoethanol at pH 7.5, 1 year, no loss of activity
-
4C, purified enzyme, stored in 20% glycerol is stable for months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni2+-NTA column chromatography
P00350
ammonium sulfate precipitation, 2',5'-ADP Sepharose 4B affinity chromatography and Sephadex G200 gel filtration
-
HisTrap column chromatography and Superdex 200 gel filtration
-
725.4fold from erythrocytes by ammonium sulfate precipitation, 2',5'-ADP-resin affinity chromatography, and gel filtration
-
Ni2+-NTA column chromatography
Q762L5
wild-type, mutant col-3, mutant col-10
-
partially, fractionation
-
Ni-NTA column chromatography and ADP-agarose column chromatography
-
Ni-NTA resin chromatography and ADP-agarose affinity column chromatography
-
Ni-NTA resin column chromatography and 2?5?ADP agarose chromatography
-
recombinant enzyme
-
78.5fold, partial from erythrocytes by ammonium sulfate precipitation, fraction 60-80% saturation
-
Sephadex G-25 column chromatography, DEAE-Sepharose Fast Flow ion exchange chromatography and 2',5'-ADP Sepharose 4B column chromatography
-
Ni2+-NTA affinity chromatography
-
purified from an overexpressing Escherichia coli strain
-
recombinant from Escherichia coli
P31072
native enzyme by partially 27fold ion exchange, affinity and size exclusion chromatography, His-tagged recombinant wild-type and mutant enzymes by nickel affinity chromatography
Q6WAT5
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
-
in Escherichia coli
Q6NHC5
the Y378C mutant is expressed in Escherichia coli BL21 (DE3) cells
P00350
expressed in Escherichia coli BL21 (DE3) pLysS cells
-
6PGDH cDNA encodes a protein with 94% sequence identity with the sheep liver enzyme
-
the A134T mutant is expressed in Escherichia coli BL21 (DE3) cells
Q762L5
DNA and amino acid sequence determination and analysis
Q7FRX8
amino acid sequence of the sheep liver enzyme deduced from cDNA clones
-
expressed in Escherichia coli M15 cells
-
expressed in Escherichia coli strain M15
-
expressed in Escherichia coli strain M15[pREP4]
-
mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase from sheep liver reverse transcribed and amplified resulting cDNA subcloned into pQE-30 and overproduced in Escherichia coli M15
-
expressed in Escherichia coli
-
expression in Escherichia coli
A4VX60
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli
-
overexpression in Escherichia coli
P31072
DNA and amino acid sequence determination and analysis, functional overexpression of soluble His-tagged wild-type and mutant enzymes in Escherichia coli
Q6WAT5
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E131A
-
the mutant shows a 2.3fold decrease in turnover number compared to the wild type enzyme
H186A
-
reduced activity compared to the wild type enzyme
H187A
-
reduced activity compared to the wild type enzyme
K260A
-
reduced activity compared to the wild type enzyme
M13C
-
mutant showing decrease in affinity for NADP+, but not NADPH
M13C
-
the mutant shows a decrease in turnover number compared to the wild type enzyme
M13F
-
mutant showing decrease in affinity for NADP+, but not NADPH
M13I
-
mutant showing decrease in affinity for NADP+, but not NADPH
M13Q
-
mutant showing decrease in affinity for NADP+, but not NADPH
M13Q
-
the mutant shows a 8.1fold decrease in turnover number compared to the wild type enzyme
M13V
-
mutant showing decrease in affinity for NADP+, but not NADPH
M13V
-
the mutant shows a decrease in turnover number compared to the wild type enzyme
R287A
-
reduced activity compared to the wild type enzyme
R446A
-
reduced activity compared to the wild type enzyme
S128A
-
reduced activity compared to the wild type enzyme
T262A
-
activity similar to the wild type enzyme
V244D/C257R
Q6WAT5
site-directed mutagenesis, unaltered reaction kinetics and increased stability, due introduction of 2 salt bridges by the double-mutation, compared to the wild-type enzyme
additional information
-, P80859
construction of a knockout mutant
Y191A
-
reduced activity compared to the wild type enzyme
additional information
P38720
truncated mutants without the C-terminal 35, 39 or 53 residues of Gnd1 completely lose their 6PGDH activity
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
thermostability may lead to some practical applications
synthesis
Geobacillus stearothermophilus NCA 1503
-
thermostability may lead to some practical applications
-
medicine
-
with a deficiency of 6-phosphogluconate dehydrogenase some reticulocytosis occurs
medicine
-
-
medicine
-
6PGDH might be a target for chemotherapy against parasitic infections as trypanosomiasis
medicine
-
6-phosphogluconate dehydrogenase is a potential target for new drugs against African trypanosomiasis
pharmacology
Q6WAT5
the enzyme is a target for inhibitor development for usage as drugs against African Trypanosomiasis