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Literature summary for 1.1.1.44 extracted from

  • Veronese, F.M.; Boccu, E.; Fontana, A.
    Isolation and properties of 6-phosphogluconate dehydrogenase from Escherichia coli. Some Comparisons with the thermophilic enzyme from Bacillus stearothermophilus (1976), Biochemistry, 15, 4026-4033.
    View publication on PubMed

General Stability

General Stability Organism
denaturation by urea Escherichia coli
stable in 8 M urea, nearly unaffected, only 10% loss of activity after exposure for 60 min Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Cyberlindnera jadinii
5,5'-dithiobis(2-nitrobenzoic acid)
-
Enterococcus faecalis
5,5'-dithiobis(2-nitrobenzoic acid)
-
Escherichia coli
5,5'-dithiobis(2-nitrobenzoic acid)
-
Geobacillus stearothermophilus
D-fructose 1,6-diphosphate
-
Enterococcus faecalis
D-fructose 1,6-diphosphate
-
Escherichia coli
D-fructose 1,6-diphosphate
-
Neurospora crassa
NADPH
-
Escherichia coli
NADPH
-
Geobacillus stearothermophilus
NADPH
-
Neurospora crassa
p-chloromercuribenzoate
-
Enterococcus faecalis
p-chloromercuribenzoate
-
Escherichia coli
p-chloromercuribenzoate
-
Geobacillus stearothermophilus
Rose bengal photooxidation Escherichia coli
Rose bengal photooxidation Geobacillus stearothermophilus
Rose bengal photooxidation Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
NADP+
-
Geobacillus stearothermophilus
0.05
-
6-phosphogluconate
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Cyberlindnera jadinii
100000
-
gel filtration Escherichia coli
100000
-
sucrose density gradient centrifugation Geobacillus stearothermophilus
106000 115000
-
Enterococcus faecalis
110000 120000
-
Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Enterococcus faecalis
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Escherichia coli enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Geobacillus stearothermophilus enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Neurospora crassa enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ Escherichia coli MRE 600 enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
MRE 600
-
Escherichia coli MRE 600
-
MRE 600
-
Geobacillus stearothermophilus
-
-
-
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
20°C, in phosphate buffer pH 6.8 in presence of the reducing agent beta-mercaptoethanol enzyme remains fully active for 24 h Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Escherichia coli D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Geobacillus stearothermophilus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Enterococcus faecalis D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Escherichia coli D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Geobacillus stearothermophilus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+
-
Escherichia coli MRE 600 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Escherichia coli MRE 600 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer
-
Cyberlindnera jadinii
dimer
-
Escherichia coli
dimer
-
Geobacillus stearothermophilus
dimer
-
Neurospora crassa
dimer
-
Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
Escherichia coli
75 80
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable below Escherichia coli
60
-
retains more than 90% of its structure Geobacillus stearothermophilus
67
-
stable below Geobacillus stearothermophilus
70
-
stable up to Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Cyberlindnera jadinii
NADP+
-
Escherichia coli
NADP+
-
Neurospora crassa
NADP+
-
Enterococcus faecalis
NADP+ strictly specific for NADP+, no activity observed with NAD+ Geobacillus stearothermophilus