Cloned (Comment) | Organism |
---|---|
the A134T mutant is expressed in Escherichia coli BL21 (DE3) cells | Klebsiella pneumoniae |
the Y378C mutant is expressed in Escherichia coli BL21 (DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, at 4°C, with 0.12 M diammonium hydrogen citrate buffer (pH 5.0) and 20% (w/v) polyethylene glycol (PEG) 3350 | Klebsiella pneumoniae |
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, using 0.1 M sodium citrate dihydrate buffer (pH5.4), 0.5 M ammonium acetate, 6-7% (w/v) PEG 3350, and 17-18% (w/v) PEG 4000 as a reservoir, at 18°C | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
NADP+ | in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C | Escherichia coli | |
0.093 | - |
6-phospho-D-gluconate | in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C | Escherichia coli | |
0.107 | - |
6-phospho-D-gluconate | in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C | Klebsiella pneumoniae | |
0.118 | - |
NADP+ | in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00350 | - |
- |
Klebsiella pneumoniae | Q762L5 | - |
- |
Klebsiella pneumoniae NTUH-K2044 | Q762L5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Escherichia coli |
Ni2+-NTA column chromatography | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | - |
Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? | |
6-phospho-D-gluconate + NADP+ | - |
Klebsiella pneumoniae | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? | |
6-phospho-D-gluconate + NADP+ | - |
Klebsiella pneumoniae NTUH-K2044 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Escherichia coli |
homodimer | x-ray crystallography | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
6-phosphogluconate dehydrogenase | - |
Escherichia coli |
6-phosphogluconate dehydrogenase | - |
Klebsiella pneumoniae |
6PGDH | - |
Escherichia coli |
6PGDH | - |
Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Klebsiella pneumoniae |