6.3.2.4: D-Alanine-D-alanine ligase

This is an abbreviated version!
For detailed information about D-Alanine-D-alanine ligase, go to the full flat file.

Word Map on EC 6.3.2.4

6.3.2.4

peptidoglycan

vancomycin

enterococci

d-cycloserine

vancomycin-resistant

faecium

d-alanyl-d-lactate

teicoplanin

depsipeptide

d-ala-d-lac

drug development

vanrs

atp-grasp

vancomycin-dependent

vand-type

d-ala-d-lactate

d-ala2

analysis

synthesis

Reaction

+ 2 D-alanine =

Synonyms

AbDDL, ATP-dependent D-Ala:D-Ala ligase, BBR47_20440, BMD_0213, BMQ_0219, BMWSH_5013, D-Ala-D-Ala ligase, D-Ala-D-Ala synthetase, D-Ala:D-Ala ligase, D-alanine:D-alanine (D-lactate) ligase (ADP), D-Alanine:D-alanine ligase, D-alanyl-D-alanine ligase B, D-Alanyl-D-alanine synthetase, D-Alanylalanine synthetase, DD1, DDI, Ddl, DdlA, DdlB, DdlB ligase, dll, dllB, EcDdlB, More, Rv2981c, SsDdl, Synthetase, D-alanylalanine, Tb-DdlA, TmDdl, TtDdL, TTHA1587, VanA, VanB ligase, YpDDL

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.2 Acid—amino-acid ligases (peptide synthases)

6.3.2.4 D-Alanine-D-alanine ligase

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Results	in table
2	Activating Compound
46866	AA Sequence
10	Application
1	CAS Registry Number
31	Cloned(Commentary)
14	Cofactor
15	Crystallization (Commentary)
3	Expression
25	General Information
48	IC50 Value
247	Inhibitors
17	kcat/KM [mM/s]
21	Ki Value [mM]
99	KM Value [mM]
13	Metals/Ions
3	Molecular Weight [Da]
45	Natural Substrates/ Products (Substrates)
399	Organism
8	Pathway
148	PDB ID
11	pH Optimum
2	pH Range
51	Protein Variants
20	Purification (Commentary)
4	Reaction
2	Reaction Type
92	Reference
4	Source Tissue
21	Specific Activity [micromol/min/mg]
167	Substrates and Products (Substrate)
15	Subunits
91	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
32	Turnover Number [1/s]

Purification

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Purification on EC 6.3.2.4 - D-Alanine-D-alanine ligase

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8 entries

overproducing strain

Salmonella enterica subsp. enterica serovar Typhimurium

1226, 1239

recombinant Ddl

Escherichia coli

650819

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

2 entries

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through TEV protease, and another step of nickel affinity chromatography, followed by desalting gel filtration, dialysis, and ultrafiltration

Yersinia pestis

Q8ZIE7

743944

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)

Helicobacter pylori

Q2N4T5

695037

recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(lambdaDE3)StarpRosetta by nickel affinity chromatography and gel filtration

Escherichia coli

P07862

745865

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by dialysis

Van A