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6.3.2.4: D-Alanine-D-alanine ligase

This is an abbreviated version!
For detailed information about D-Alanine-D-alanine ligase, go to the full flat file.

Word Map on EC 6.3.2.4

Reaction

ATP
+ 2 D-alanine =
ADP
 +
phosphate
 +
D-alanyl-D-alanine

Synonyms

AbDDL, ATP-dependent D-Ala:D-Ala ligase, BBR47_20440, BMD_0213, BMQ_0219, BMWSH_5013, D-Ala-D-Ala ligase, D-Ala-D-Ala synthetase, D-Ala:D-Ala ligase, D-alanine:D-alanine (D-lactate) ligase (ADP), D-Alanine:D-alanine ligase, D-alanyl-D-alanine ligase B, D-Alanyl-D-alanine synthetase, D-Alanylalanine synthetase, DD1, DDI, Ddl, DdlA, DdlB, DdlB ligase, dll, dllB, EcDdlB, More, Rv2981c, SsDdl, Synthetase, D-alanylalanine, Tb-DdlA, TmDdl, TtDdL, TTHA1587, VanA, VanB ligase, YpDDL

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.4 D-Alanine-D-alanine ligase

Cloned

Cloned on EC 6.3.2.4 - D-Alanine-D-alanine ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ddl is amplified from Streptococcus mutans genomic DNA and cloned into the expression vector pET28a. The protein is expressed in soluble form in Escherichia coli strain BL21 (DE3)
-
expressed in Escherichia coli ATM697 and ATM718 as a fusion protein MurC-Ddl
-
expressed in Escherichia coli BL21
-
expression in Escherichia coli
expression in Escherichia coli BL21
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
-
expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene ddl, recombinant expression
gene ddlA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ddlB, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(lambdaDE3)StarpRosetta
gene dll, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene dll, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS
gene dll, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
gene dll, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene dll, the ddlR gene is located just upstream of the D-alanyl-D-alanine ligase gene (ddl) in the Brevibacillus brevis genome and the two genes dll and dllR form an operon. Enzyme DllR acts as a transcription factor, and purified DdlR binds specifically to the DNA region that includes putative -35 and -10 regions of the ddlR promoter, a 6-bp inverted repeat that overlaps the -10 region of the ddlR promoter is important for the binding. Phylogenetic tree based on the amino acid sequences of D-alanyl-D-alanine ligases (Ddls) in the proteomes of Escherichia coli and ddlR-positive bacteria
overexpression of Ddl in Mycobacterium smegmatis
overproduction in Escherichia coli, the vancomycin resistant enzyme has Phe261 instead of Tyr. The Tyr to Phe substitution on the active site omega-loop in the D-Ala-D-Ala ligases is a molecular indicator of both the ability to make D-Ala-D-Lac and intrinsic resistance to the vancomycin class of glycopeptide antibiotics
placed under the control of the lac and lambdaPl promoter
-
subcloned behind the tac promoter in the plasmid pKK223-3, expression in Escherichia coli JM105
-
Van B ligase is overproduced in Escherichia coli HB101 harboring recombinant plasmid pAT204
-