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6.2.1.30: phenylacetate-CoA ligase

This is an abbreviated version!
For detailed information about phenylacetate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.30

Reaction

ATP
+
phenylacetate
+
CoA
=
AMP
+
diphosphate
+
phenylacetyl-CoA

Synonyms

EC 6.2.1.21, More, PA-CoA ligase, PAA-CoA ligase, PaaF2, PaaK1, PaaK2, PCL, phenyl/phenoxyacetic acid activating enzyme, phenyl/phenoxyacetyl-CoA-ligase, phenylacetyl CoA ligase, phenylacetyl-CoA ligase, phenylacetyl-CoA ligase (AMP-forming), phenylacetyl-coenzyme A ligase, Phl protein, phlB, PhlC, styrene-specific PA-CoA ligase, synthetase, phenacyl coenzyme A

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.30 phenylacetate-CoA ligase

General Stability

General Stability on EC 6.2.1.30 - phenylacetate-CoA ligase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes
-
enzyme is rather labile and requires the presence of glycerol and/or phenylacetate for stabilization
-
presence of 200 mM NaCl is essential for the stability of the enzyme and storage or conversions in buffer lacking NaCl resulted in rapid loss of activity
purified enzyme is sensitive to repeated freezing
repeated freezing and thawing inactivates
-
sucrose stabilizes
-