6.2.1.30: phenylacetate-CoA ligase

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For detailed information about phenylacetate-CoA ligase, go to the full flat file.

Reaction

Synonyms

EC 6.2.1.21, More, PA-CoA ligase, PAA-CoA ligase, PaaF2, PaaK1, PaaK2, PCL, phenyl/phenoxyacetic acid activating enzyme, phenyl/phenoxyacetyl-CoA-ligase, phenylacetyl CoA ligase, phenylacetyl-CoA ligase, phenylacetyl-CoA ligase (AMP-forming), phenylacetyl-coenzyme A ligase, Phl protein, phlB, PhlC, styrene-specific PA-CoA ligase, synthetase, phenacyl coenzyme A

ECTree

     6 Ligases

         6.2 Forming carbon-sulfur bonds

             6.2.1 Acid-thiol ligases

                6.2.1.30 phenylacetate-CoA ligase

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Results	in table
4	Activating Compound
13416	AA Sequence
3	Application
1	CAS Registry Number
14	Cloned(Commentary)
6	Cofactor
2	Crystallization (Commentary)
8	Expression
27	General Information
7	General Stability
30	Inhibitors
7	kcat/KM [mM/s]
1	Ki Value [mM]
41	KM Value [mM]
13	Localization
17	Metals/Ions
12	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
108	Organism
1	Oxidation Stability
11	Pathway
6	PDB ID
8	pH Optimum
3	pH Range
4	pI Value
12	Protein Variants
10	Purification (Commentary)
1	Reaction
2	Reaction Type
50	Reference
16	Source Tissue
15	Specific Activity [micromol/min/mg]
8	Storage Stability
113	Substrates and Products (Substrate)
13	Subunits
32	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
29	Turnover Number [1/s]

Crystallization

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Crystallization on EC 6.2.1.30 - phenylacetate-CoA ligase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

adenylated phenylacetate intermediate complex of purified recombinant His-tagged PaaK2, sitting drop vapor diffusion method, 12 mg/ml protein in 20 mM HEPES, pH 8.3, 10 mM NaCl, 3 mM 2-mercaptoethanol, 2% glycerol, 3 mM MgCl2, and 3 mM ATP, is mixed with 17% w/v PEG 6000, 0.1 M HEPES, pH 7.5, 0.1 M KCl, and 2.5% glycerol, X-ray diffraction structure determination and analysis at 1.90 A resolution

Burkholderia cenocepacia

B4E7B5, B4EL89

727874

purified recombinant His-tagged selenomethionine derivative of PaaK1 in complex with ATP and adenylated phenylacetate intermediate complex of purified recombinant His-tagged PaaK1, sitting drop vapor diffusion method, 12 mg/ml protein in 20 mM HEPES, pH 8.3, 10 mM NaCl, 3 mM 2-mercaptoethanol, 2% glycerol, 3 mM MgCl2, and 3 mM ATP, is mixed with 20-25% PEG 3350, 200 mM potassium thiocyanate, and 5% glycerol. Crystals of PaaK1 are grown from 10% w/v PEG 8000, 0.1 M HEPES, pH 7.5, and 8.0% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 1.6 A and 1.92 A resolution, respectively

Burkholderia cenocepacia

B4E7B5, B4EL89

727874