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6.2.1.30: phenylacetate-CoA ligase

This is an abbreviated version!
For detailed information about phenylacetate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.30

Reaction

ATP
+
phenylacetate
 +
CoA
=
AMP
 +
diphosphate
 +
phenylacetyl-CoA

Synonyms

EC 6.2.1.21, More, PA-CoA ligase, PAA-CoA ligase, PaaF2, PaaK1, PaaK2, PCL, phenyl/phenoxyacetic acid activating enzyme, phenyl/phenoxyacetyl-CoA-ligase, phenylacetyl CoA ligase, phenylacetyl-CoA ligase, phenylacetyl-CoA ligase (AMP-forming), phenylacetyl-coenzyme A ligase, Phl protein, phlB, PhlC, styrene-specific PA-CoA ligase, synthetase, phenacyl coenzyme A

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.30 phenylacetate-CoA ligase

Storage Stability

Storage Stability on EC 6.2.1.30 - phenylacetate-CoA ligase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -70°C, 70% loss of activity after 1 week
-
-20°C, 10% glycerol, activity is retained for 2 months without remarkable loss of activity
-
-20°C, crude enzyme is stable in presence of glycerol for 2-3 weeks, more than 50% loss of activity of the purified enzyme
-
-20°C, purified recombinant protein in TANG buffer, several months without significant loss of activity
0°C, 24 h, very little activity retained, sucrose and 2-mercaptoethanol stabilize
-
4°C, 10% glycerol, 65% of the activity is recovered after 72 h
-
4°C, 90% loss of activity after 1 day without glycerol, plus 10% glycerol, half-life: 2 days, plus 5 mM phenylacetate, half-life: 1 week, plus 35% glycerol, half-life: 2 weeks
-
4°C, purified recombinant enzyme, 72 h no loss of activity, in the presence of 10% glycerol stable for up to 3 weeks