6.2.1.30: phenylacetate-CoA ligase

This is an abbreviated version!
For detailed information about phenylacetate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.30

6.2.1.30

phenylacetic

penicillin

chrysogenum

putida

ligases

isopenicillin

cenocepacia

phenylglyoxylate

benzoyl-coa

denitrifying

synthesis

3-hydroxyphenylacetic

phenylacetaldehyde

analysis

Reaction

Synonyms

EC 6.2.1.21, More, PA-CoA ligase, PAA-CoA ligase, PaaF2, PaaK1, PaaK2, PCL, phenyl/phenoxyacetic acid activating enzyme, phenyl/phenoxyacetyl-CoA-ligase, phenylacetyl CoA ligase, phenylacetyl-CoA ligase, phenylacetyl-CoA ligase (AMP-forming), phenylacetyl-coenzyme A ligase, Phl protein, phlB, PhlC, styrene-specific PA-CoA ligase, synthetase, phenacyl coenzyme A

ECTree

6 Ligases

6.2 Forming carbon-sulfur bonds

6.2.1 Acid-thiol ligases

6.2.1.30 phenylacetate-CoA ligase

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Results	in table
4	Activating Compound
13416	AA Sequence
3	Application
1	CAS Registry Number
14	Cloned(Commentary)
6	Cofactor
2	Crystallization (Commentary)
8	Expression
27	General Information
7	General Stability
30	Inhibitors
7	kcat/KM [mM/s]
1	Ki Value [mM]
41	KM Value [mM]
13	Localization
17	Metals/Ions
12	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
108	Organism
1	Oxidation Stability
11	Pathway
6	PDB ID
8	pH Optimum
3	pH Range
4	pI Value
12	Protein Variants
10	Purification (Commentary)
1	Reaction
2	Reaction Type
50	Reference
16	Source Tissue
15	Specific Activity [micromol/min/mg]
8	Storage Stability
113	Substrates and Products (Substrate)
13	Subunits
32	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
29	Turnover Number [1/s]

Purification

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Purification on EC 6.2.1.30 - phenylacetate-CoA ligase

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4 entries

recombinant C-terminally His6-tagged maltose binding protein fusion from Escherichia coli by nickel affinity chromatography, the MBP is cleaved off by factor Xa, imidazole is eliminated by gel filtration, recombinant His-tagged mutants

Penicillium chrysogenum

O74725

690837

recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 (DE3)-RP by nickel affinity chromatography

Penicillium chrysogenum

D3GE78

727565

recombinant His-tagged isozyme PaaK1 from Escherichia coli strain BL21Star(DE3) and selenomethionine-labeled His-tagged PaaK1 from Escherichia coli strain 834(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography

Burkholderia cenocepacia

B4E7B5, B4EL89

727874

recombinant His-tagged isozyme PaaK2 from Escherichia coli strain BL21Star(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography

Burkholderia cenocepacia

B4E7B5, B4EL89

727874

recombinant His6-tagged enzyme from Escherichia coli

strain KB 740